Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers

In many African countries accurate and reliable identification of poached wildlife products like carcasses or meat presents a big problem when morphological characters such as skin hair or bones are missing. We describe a molecular based approach that has a potential of serving as a forensic tool in game meat identification in Africa. A mitochondial DNA marker (mt700) and one restriction enzyme, Rsa1 were used in the PCR-RFLP species identification of game meat obtained from two National Parks in Tanzania. Species-specific reference DNA fragment patterns were obtained using fresh meat from ten wildlife and four domesticated species. All species except the zebra, produced unique monomorphic RFLP patterns. Collectively, these patterns demonstrate the potential ability of genetic techniques for discriminating between and among wildlife and domestic species. The reference PCR-RFLP fragments enabled species identification of about 79% of unknown meat samples. In addition, sex was alsoassigned to all of the samples following successful amplification of gender-specific, SRY and ZFY/X, chromosomal domains. Although the present study has been conducted on a limited range both in numbers and genetic diversity of wildlife species present in Africa, the results demonstrate thepotential usefulness of the DNA approach in wildlife forensics in the continent

Relaksasi Autogenik Terhadap Penurunan Skala Nyeri Pada Ibu Post Operasi Sectio Saecarea

Introduction: Sectio Caesarea is defined as the delivery method of a fetus through surgical incisions which made in the abdominal wall (laparotomy) and the uterine wall (hysterotomy). One of the complications of sectio caesarea is pain in the incision area. A non-pharmacological pain management strategies to overcome pain is autogenic relaxation therapy. Objective: The aim of the research was to evaluate the effect of autogenic relaxation to decrease pain scale on postoperative mother undergoing Sectio Caesarea (SC) in the V/VI ward Tk.II Dustira Cimahi Hospital. Method: Research design used was experimental research with One Group Pretest Posttest Design involving 75 post sectio caesarea mother within 1 month. Sampling technique used was Non Probability Sampling namely Purposive Sampling techniques. Result: Postoperative pain scale before the intervention 64% of respondents experienced a post-operative incision pain with range scale of 4-6 (moderate pain), while post-operative pain scale after the intervention 73.3% of respondents experienced pain with range scale of 4-6 (moderate pain). There is a significant effect of autogenic relaxation with decreased pain scale. T-test results showed 0.0001 means that there are differences between the pain scale before and after autogenic relaxation with a mean = 1,080 ie the pain scale tendentiously decreased after treatment with an average reduction in pain scale is 1,080

Dhfr and dhps mutations in Plasmodium falciparum isolates in Mlandizi, Kibaha,

Sulfadoxine-pyrimethamine (SP), the current first line antimalarial drug in Tanzania, is compromised by evolution and spread of mutations in the parasite's dhfr and dhps genes. In the present study we established the baseline frequencies of Plasmodium falciparum dihydrofolate reductase (pfdhfr) and dihydropteroate synthase (pfdhps) mutant genotypes and their potential for predicting the in vivo efficacy of SP in Mlandizi, Tanzania. The efficacy of SP treatment was by following 116 children with uncomplicated falciparum malaria for 14 days after treatment. Infected blood samples were collected on filter paper at days 0, 3, 7 and 14. Parasite genomic DNA was extracted and point mutations at positions 51, 59, 108 and 164 of the dhfr gene and at 581, 540 and 437 of the dhps gene were analysed by nested Polymerase Chain Reaction/ Restriction Fragment Length Polymorphism. Out of 116 children enrolled, 98 (86%) of eligible children demonstrated an adequate clinical response by day 14. There were 7.3 % early and 6.7% late therapeutic failures. At day 0, only 8.0% (4/50) the parasites showed no mutation at the dhfr locus; for dhps this was 73%. Triple mutant dhfr alleles (Ile 51, Arg 59, Asn 108) occurred in 47%, double mutant dhps (Gly 437, Glu 540) alleles in 7.9%. No mutation was detected at codon 164 of the dhfr gene. The presence of triple dhfr mutant alleles was related to clinical failure, but did not show significant association (Fisher exact test, P=0.166, OR 2.15 0.776.20). The higher rates of mutation on the dhfr do not spell a bright future for SP treatment in Tanzania. It is rational to think of an alternative first line antimalarial drug, while retaining SP for malaria intermittent treatment in regnancy. Keywords: Plasmodium falciparum, sulfadoxine-pyrimethamine, dhfr, dhps, TanzaniaTanzania Health Research Bulletin Vol. 8 (2) 2006: pp. 50-5

The Use of Chemistry of Garnets and Heavy Minerals Around Lalago Kimberlite Pipe in Deciphering Diamond and Non-Diamond Bearing Kimberlite Pipes in Tanzania

More than three hundred kimberlite pipes have been reported in Tanzania. Only a few are diamond–bearing. A prospecting criteria to outline the diamond and non-diamond bearing kimberlites has been proposed. Bulk rock chemical analyses and chemistry of garnets and black minerals (picroilmenite, magnetite, rutile and titanite) collected around one kimberlite pipe in Tanzania were studied using Atomic Absorption Spectrophotometer (AAS) and Electron Microprobe (EMP). Although chromite and zircons occur in kimberlite pipes, they were not used in this study because they also characterize other surrounding rocks. Electron microprobe analysis of heavy minerals indicate that the ilmenites (picroilmenite) are poor in MgO contents (0.03 – 0.6 wt.%); but are rich in MnO (9.94 – 12.27wt.%). The garnets are poor in Cr2O3 with pronounced almandine content which has led to the conclusion of having a barren kimberlite source. It is suggested that combination of the chemistry of garnet and heavy minerals may be used as an exploration tool for deciphering diamond and non-diamond bearing kimberlites.Keywords: Electron microprobe, black minerals, mineral and fluid inclusions, kimberlites, garnets

Capsular bag distension syndrome following phacoemulsification with implantation of intraocular lens

Capsular bag distension syndrome is a rare complication following phacoemulsification with posterior chamber intraocular lens implantation. We describe the case of a middle-age male, who developed early onset capsular bag distension syndrome of the right eye after phacoemulsification with implantation of plate haptic posterior chamber intraocular lens (Zeiss CT ASPHINA 509MP). There was persistent poor vision during follow up at 1 week and 5 weeks post-surgery with a myopic shift of 2.5 diopters sphere (DS). The intraocular pressure was only elevated at 2 hours post-operative and was normal during the subsequent followup. Distension of capsular bag was confirmed with the IOP Master 700 which showed abnormal separation of posterior capsule from the intraocular lens. He was diagnosed with capsular bag distension syndrome secondary to retention of viscoelastic. Right eye removal of retained viscoelastic was performed and the capsular bag distension syndrome resolved successfully with best corrected visual acuity at 6/6. Hence, complete clearance of viscoelastic during phacoemulsification with plate haptic intraocular lens is important in order to prevent capsular bag distension syndrome

Thermal Decomposition of CxF2 x+1C(O)OONO2(x = 2, 3, 4)

The atmospheric degradation of molecules containing the CxF2x+1C(O) moiety, such as perfluoroaldehydes CxF2x+1C(O)H (x = 2-4) formed in the degradation of telomeric alcohols, could lead to the formation of perfluoroacyl peroxynitrates CxF2x+1C(O)OONO2. The thermal decomposition of the CxF2x+1C(O)OONO2 family (x = 2, 3, 4) was investigated by infrared spectroscopy and computational models. Each peroxynitrate synthesis was performed through the photolysis of gas mixtures of the corresponding perfluoroaldehyde, chlorine, nitrogen dioxide, and oxygen. Kinetic analysis for the thermal decomposition of peroxynitrates were performed in the range from 297.0 to 313.7 K at a total pressure of 1000 mbar and the activation energy was experimentally determined. Experimental data were complemented with theoretical data using the Gaussian09 Program Suite. The structures of peroxynitrates were optimized using DFT methods. The activation energies were calculated and investigated taking into account the stereoelectronic effects and using theoretical calculations as well as NBO analysis. The influence of anomeric interaction over the O-N bond was evaluated for all the molecules. Analysis of the results shows that CxF2x+1C(O)OONO2 stability is independent of CxF2x+1 chain length, in contrast to the behavior for perfluoroalkyl peroxynitrates (CxF2x+1OONO2).Fil: Vila, Jesús Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Iriarte, Ana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Chiappero, Malisa Susana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Malanca, Fabio Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentin

Recurrent orbital cellulitis secondary to the ‘forgotten’ scleral buckle: a case report

Scleral buckle placement is a well-established technique for the treatment of primary rhegmatogenous retinal detachment. Complications associated with scleral buckle are uncommon and its presentations can be vary. We report a case of recurrent orbital cellulitis with anterior segment ischemia following a forgotten episode of previous scleral buckling surgery, presenting with blurring of vision, redness and swelling of the lids. The presence of scleral buckle was detected by detailed examination and confirmed by orbital imaging. Orbital infection and rubeosis iridis were successfully treated with scleral buckle removal, intravenous antibiotics and intracameral ranibizumab. However, the retinal detachment recurred and the visual acuity deteriorated to light perception. There was no further intervention as the family declined in view of her old age. In cases of recurrent orbital infection, detailed clinical examination is important to look for evidence of ocular prostheses as a source of infection. Orbital imaging is an adjunct for making the diagnosis especially in cases where history is unreliable. Anterior segment ischemia due to scleral buckle responds well to buckle removal with ranibizumab injection

Ectopic Expression Of Pericentric HSATII RNA Results In Nuclear RNA Accumulation, MeCP2 Recruitment, And Cell Division Defects

Within the pericentric regions of human chromosomes reside large arrays of tandemly repeated satellite sequences. Expression of the human pericentric satellite HSATII is prevented by extensive heterochromatin silencing in normal cells, yet in many cancer cells, HSATII RNA is aberrantly expressed and accumulates in large nuclear foci in cis. Expression and aggregation of HSATII RNA in cancer cells is concomitant with recruitment of key chromatin regulatory proteins including methyl-CpG binding protein 2 (MeCP2). While HSATII expression has been observed in a wide variety of cancer cell lines and tissues, the effect of its expression is unknown. We tested the effect of stable expression of HSATII RNA within cells that do not normally express HSATII. Ectopic HSATII expression in HeLa and primary fibroblast cells leads to focal accumulation of HSATII RNA in cis and triggers the accumulation of MeCP2 onto nuclear HSATII RNA bodies. Further, long-term expression of HSATII RNA leads to cell division defects including lagging chromosomes, chromatin bridges, and other chromatin defects. Thus, expression of HSATII RNA in normal cells phenocopies its nuclear accumulation in cancer cells and allows for the characterization of the cellular events triggered by aberrant expression of pericentric satellite RNA

T Cells Specific for a Mycobacterial Glycolipid Expand after Intravenous Bacillus Calmette-Guérin Vaccination

Intradermal vaccination with Mycobacterium bovis bacillus Calmette-Guérin (BCG) protects infants from disseminated tuberculosis, and i.v. BCG protects nonhuman primates (NHP) against pulmonary and extrapulmonary tuberculosis. In humans and NHP, protection is thought to be mediated by T cells, which typically recognize bacterial peptide Ags bound to MHC proteins. However, during vertebrate evolution, T cells acquired the capacity to recognize lipid Ags bound to CD1a, CD1b, and CD1c proteins expressed on APCs. It is unknown whether BCG induces T cell immunity to mycobacterial lipids and whether CD1-restricted T cells are resident in the lung. In this study, we developed and validated Macaca mulatta (Mamu) CD1b and CD1c tetramers to probe ex vivo phenotypes and functions of T cells specific for glucose monomycolate (GMM), an immunodominant mycobacterial lipid Ag. We discovered that CD1b and CD1c present GMM to T cells in both humans and NHP. We show that GMM-specific T cells are expanded in rhesus macaque blood 4 wk after i.v. BCG, which has been shown to protect NHP with near-sterilizing efficacy upon M. tuberculosis challenge. After vaccination, these T cells are detected at high frequency within bronchoalveolar fluid and express CD69 and CD103, markers associated with resident memory T cells. Thus, our data expand the repertoire of T cells known to be induced by whole cell mycobacterial vaccines, such as BCG, and show that lipid Ag-specific T cells are resident in the lungs, where they may contribute to protective immunity

Fitness Trade-Offs in the Evolution of Dihydrofolate Reductase and Drug Resistance in Plasmodium falciparum

Background: Patterns of emerging drug resistance reflect the underlying adaptive landscapes for specific drugs. In Plasmodium falciparum, the parasite that causes the most serious form of malaria, antifolate drugs inhibit the function of essential enzymes in the folate pathway. However, a handful of mutations in the gene coding for one such enzyme, dihydrofolate reductase, confer drug resistance. Understanding how evolution proceeds from drug susceptibility to drug resistance is critical if new antifolate treatments are to have sustained usefulness. Methodology/Principal Findings: We use a transgenic yeast expression system to build on previous studies that described the adaptive landscape for the antifolate drug pyrimethamine, and we describe the most likely evolutionary trajectories for the evolution of drug resistance to the antifolate chlorcycloguanil. We find that the adaptive landscape for chlorcycloguanil is multi-peaked, not all highly resistant alleles are equally accessible by evolution, and there are both commonalities and differences in adaptive landscapes for chlorcycloguanil and pyrimethamine. Conclusions/Significance: Our findings suggest that cross-resistance between drugs targeting the same enzyme reflect the fitness landscapes associated with each particular drug and the position of the genotype on both landscapes. The possibl