Investigation of the free flow electrophoretic process

The effects of gravity on the free flow electrophoretic process was demonstrated. The free flow electrophoresis chamber used to demonstrate the effects of gravity on the process was of a proprietary design. This chamber was 120 cm long, 16 cm wide, and 0.15 cm thick. Flow in this chamber was in the upward direction and exited through 197 outlets at the top of the chamber. During electrophoresis a stream of sample was injected into the flow near the bottom of the chamber and an electrical field was applied across the width of the chamber. The field caused a lateral force on particles in the sample proportional to the inherent change of the particle and the electric field strength. Particle lateral velocity was then dependent on the force due to viscous drag which was proportional to particle size and particle shape dependent

Investigation of the free flow electrophoretic process. Volume 1: Executive summary

The effect of gravity on the free flow electrophoretic process was investigated. The demonstrated effects were then compared with predictions made by mathematical models. Results show that the carrier buffer flow was affected by gravity induced thermal convection and that the movement of the separating particle streams was affected by gravity induced buoyant forces. It was determined that if gravity induced buoyant forces were included in the mathematical models, then effective predictions of electrophoresis chamber separation performance were possible

Postgraduate Symposium Positive influence of nutritional alkalinity on bone health: Conference on ‘Over- and undernutrition: challenges and approaches'

There is growing evidence that consumption of a Western diet is a risk factor for osteoporosis through excess acid supply, while fruits and vegetables balance the excess acidity, mostly by providing K-rich bicarbonate-rich foods. Western diets consumed by adults generate approximately 50-100 mEq acid/d; therefore, healthy adults consuming such a diet are at risk of chronic low-grade metabolic acidosis, which worsens with age as a result of declining kidney function. Bone buffers the excess acid by delivering cations and it is considered that with time an overstimulation of this process will lead to the dissolution of the bone mineral content and hence to reduced bone mass. Intakes of K, Mg and fruit and vegetables have been associated with a higher alkaline status and a subsequent beneficial effect on bone health. In healthy male volunteers an acid-forming diet increases urinary Ca excretion by 74% and urinary C-terminal telopeptide of type I collagen (C-telopeptide) excretion by 19% when compared with an alkali (base-forming) diet. Cross-sectional studies have shown that there is a correlation between the nutritional acid load and bone health measured by bone ultrasound or dual-energy X-ray absorptiometry. Few studies have been undertaken in very elderly women (>75 years), whose osteoporosis risk is very pertinent. The EVAluation of Nutrients Intakes and Bone Ultra Sound Study has developed and validated (n 51) an FFQ for use in a very elderly Swiss population (mean age 80·4 (sd 2·99) years), which has shown intakes of key nutrients (energy, fat, carbohydrate, Ca, Mg, vitamin C, D and E) to be low in 401 subjects. A subsequent study to assess net endogenous acid production (NEAP) and bone ultrasound results in 256 women aged ≥75 years has shown that lower NEAP (P=0·023) and higher K intake (P=0·033) are correlated with higher bone ultrasound results. High acid load may be an important additional risk factor that may be particularly relevant in very elderly patients with an already-high fracture risk. The latter study adds to knowledge by confirming a positive link between dietary alkalinity and bone health indices in the very elderly. In a further study to complement these findings it has also been shown in a group of thirty young women that in Ca sufficiency an acid Ca-rich water has no effect on bone resorption, while an alkaline bicarbonate-rich water leads to a decrease in both serum parathyroid hormone and serum C-telopeptide. Further investigations need to be undertaken to study whether these positive effects on bone loss are maintained over long-term treatment. Mineral-water consumption could be an easy and inexpensive way of helping to prevent osteoporosis and could be of major interest for long-term prevention of bone los

Maternal obesity during pregnancy and lactation influences offspring obesogenic adipogenesis but not developmental adipogenesis in mice

Obesity is an escalating health crisis of pandemic proportions and by all accounts it has yet to reach its peak. Growing evidence suggests that obesity may have its origins in utero. Recent studies have shown that maternal obesity during pregnancy may promote adipogenesis in offspring. However, these studies were largely based on cell culture models. Whether or not maternal obesity impacts on offspring adipogenesis in vivo remains to be fully established. Furthermore, in vivo adipogenic differentiation has been shown to happen at distinct time periods, one during development (developmental adipogenesis—which is complete by 4 weeks of age in mice) and another in adulthood in response to feeding a high-fat (HF) diet (obesogenic adipogenesis). We therefore set out to determine whether maternal obesity impacted on offspring adipocyte hyperplasia in vivo and whether maternal obesity impacted on developmental or obesogenic adipogenesis, or both. Our findings reveal that maternal obesity is associated with enhanced obesogenic adipogenesis in HF-fed offspring. Interestingly, in newly weaned (4-week-old) offspring, maternal obesity is associated with adipocyte hypertrophy, but there were no changes in adipocyte number. Our results suggest that maternal obesity impacts on offspring obesogenic adipogenesis but does not affect developmental adipogenesis

Very high prevalence of 25-hydroxyvitamin D deficiency in 6433 UK South Asian adults : Analysis of the UK Biobank Cohort

Acknowledgements This research has been conducted using the UK Biobank Resource under application number 15168. This work was supported by in-house funds from the University of Surrey for payment of the UK Biobank access fee. The UK Biobank was established by the Wellcome Trust medical charity, Medical Research Council, Department of Health, Scottish Government and the Northwest Regional Development Agency. It has also had funding from the Welsh Assembly Government and the British Heart Foundation. UK Biobank is hosted by the University of Manchester and supported by the National Health Service (NHS). All the above funders had no role in the design, analysis or writing of the present study. Author contributions were as follows: Formulating the research question(s) (A. L. D., D. J. B., K. R. A., S. L. N.), designing the study (A. L. D., D. J. B., K. R. A., S. A. L.-N.), data collection (not applicable), analysing the data (A. L. D., D. J. B., K. R. A., S. A. L.-N.) and writing the article (A. L. D., D. J. B., K. R. A., S. L. N.). S. A. L.-N. discloses that she is Research Director of D3-TEX limited which holds the UK and Gulf Corporation Council (GCC) patents for the use of UVB transparent clothing to prevent vitamin D deficiency. S. A. L.-N.’s husband William Lanham-New is Managing Director of D3-TEX limited. S. A. L.-N. has received grants from (1) The UK Biotechnology and Biological Sciences Research Council (BBSRC) (project: Ergocalciferol (D2) v. Cholecalciferol (D3) Food Fortification: Comparative Efficiency in Raising 25OHD Status & Mechanisms of Action (D2–D3 Study), BB/I006192/1, £516 823); (2) The UK Food Standards Agency (Project: Vitamin D, Food Intake, Nutrition and Exposure to Sunlight in Southern England (D-FINES) Study, N05064, £600 000); (3) The European Union (Project: Food Based Solutions for optimal vitamin D nutrition and health through the life cycle, Lead Work Package; (4) nutritional requirements for vitamin D during pregnancy, childhood and adolescence using RCTs, FP7-613977-ODIN, Euro 6·2 million) and (5) The UK Ministry of Defence (MoD, £2·4 million). S. L. N. is a current member of the Scientific Advisory Committee for Nutrition (SACN) and a member of the panel who was responsible for the most recent revision of vitamin D recommended nutritional intake guidelines in the UK. She is a board member for the UK Royal Osteoporosis Society and the British Nutrition Foundation. She is Secretary of the Nutrition Society as well as Editor in Chief of the Nutrition Society textbook series. All other authors have no conflict of interest.Peer reviewedPublisher PD

Pancreas deficiency modifies bone development in the ovine fetus near term.

Hormones have an important role in the regulation of fetal growth and development, especially in response to nutrient availability in utero. Using micro-CT and an electromagnetic three-point bend test, this study examined the effect of pancreas removal at 0.8 fraction of gestation on the developing bone structure and mechanical strength in fetal sheep. When fetuses were studied at 10 and 25 days after surgery, pancreatectomy caused hypoinsulinaemia, hyperglycaemia and growth retardation which was associated with low plasma concentrations of leptin and a marker of osteoclast activity and collagen degradation. In pancreatectomized fetuses compared to control fetuses, limb lengths were shorter, and trabecular (Tb) bone in the metatarsi showed greater bone volume fraction, Tb thickness, degree of anisotropy and porosity, and lower fractional bone surface area and Tb spacing. Mechanical strength testing showed that pancreas deficiency was associated with increased stiffness and a greater maximal weight load at fracture in a subset of fetuses studied near term. Overall, pancreas deficiency in utero slowed the growth of the fetal skeleton and adapted the developing bone to generate a more compact and connected structure. Maintenance of bone strength in growth-retarded limbs is especially important in a precocial species in preparation for skeletal loading and locomotion at birth