Epilobium hirsutum alters xenobiotic metabolizing CYP1A1, CYP2E1, NQO1 and GPx activities, mRNA and protein levels in rats

Context: Natural products have attracted increasing interests due to their use in flavoring, nutrition, cosmetics, pharmacy and medicine. Epilobium hirsutum L. (Onagraceae) is known for its analgesic, antimicrobial, and antiproliferative activity. CYP1A1 and CYP2E1, xenobiotic metabolizing enzymes, serve as a metabolic activation route yielding reactive metabolites that are eliminated by the action of NQO1 and glutathione peroxidase (GPx) enzymes. Objective: This study investigated in vivo effects of Epilobium hirsutum (EH) on CYP2E1, CYP1A1, NQO1 and GPx activities, protein and mRNA expressions in liver. Materials and methods: Male Wistar Albino rats were injected with EH at a dose of 37.5mg/kg i.p. daily for 9d. CYP2E1, CYP1A1, NQO1 and GPx activities, protein and mRNA levels were determined by enzyme assays, Western blotting and qPCR, respectively. Results: CYP1A1 associated ethoxyresorufin-O-deethylase activity of control and EH-treated animals were found as 6.54±1.21 and 4.48±1.67nmol/min/mg, respectively. CYP2E1 associated aniline 4-hydroxylase of control and EH group were 0.537±0.011 and 0.109±0.01nmol/min/mg, respectively. However, EH treatment increased the GPx and NQO1 activities from 0.069±0.015 to 0.107±0.026nmol/min/mg and from 163.34±92 to 588.3±14nmol/min/mg, respectively. Furthermore, protein and mRNA expression analysis revealed that CYP1A1 and CYP2E1 levels were decreased while those of NQO1 and GPx increased after EH treatment. Discussion and conclusion: Our current data suggest that the metabolism of xenobiotics, including drugs, may be altered due to changes in the expression and activity of these proteins by EH. © 2013 Informa Healthcare USA, Inc

Record of Lessepsian marbled spinefoot Siganus rivulatus Forsskal and Niebuhr, 1775 from the Northern Aegean Sea (Izmir Bay, Turkey)

WOS: 00031622390002

Procedia Social and Behavioral Sciences

The aim of the present study was to determine mathematics teachers' opinions about the renewed fifth grade mathematics curriculum and to investigate the new mathematics curriculum and the former one comparatively. In the current study, two qualitative research methods namely, document review and semi-structured interview methods were used. With document review, the similarities and differences between the renewed fifth grade mathematics program and the former one were discussed by comparing the two curriculums. The related documents were derived from mathematics curriculum that was published in Turkish Education Board official website. With semi structured interview form, teachers' opinions about the new curriculum was determined. In the present study, in which the purposeful sampling was used, 18 mathematics teachers were interviewed that worked at schools depending on the Ministry of Education in Sakarya province Ferizli district. The obtained data was analyzed via descriptive statistics. According to findings, the new mathematics curriculum and the former one were similar in general, and teachers had both positive and negative opinions about the new curriculum. (C) 2015 The Authors. Published by Elsevier Ltd

Synthesis of novel acridine-sulfonamide hybrid compounds as acetylcholinesterase inhibitor for the treatment of alzheimer’s disease

In this study we report that amino acridine intermediates 7 and 8 were obtained from the reduction of nitro acridine derivatives 5 and 6 that were synthesized via the condensation of dimedone, p-nitrobenzaldehyde with various amine derivatives, respectively. Then acridine sulfonamide hybrid compounds (9–18) were synthesized by the reaction of amino acridine 7, 8 with sulfonyl chlorides. The new hybrid compounds were characterized by FT-IR, 1H-NMR, 13C-NMR, and HRMS analyzes. The evaluation of in vitro anticholinesterase action of the synthesized compounds against AChE showed that some of them are potent inhibitors. Among them, compound 17 showed the most potent activity against AChE with an IC50 of 0.14 µM. © 2017, Springer Science+Business Media, LLC

The Combination of Low-dose Levobupivacaine and Fentanyl for Spinal Anaesthesia in Ambulatory Inguinal Herniorrhaphy

This study investigated whether the addition of 25 mu g intrathecal fentanyl to levobupivacaine spinal anaesthesia for outpatient inguinal herniorrhaphy allows a sub-anaesthetic levobupivacaine dose to be used. Forty patients were assigned to receive 5 mg levobupivacaine 0.5% mixed with 25 mu g fentanyl (group LF) or 7.5 mg levobupivacaine 0.5% (group L). The highest sensory block levels achieved were T7 (range T5 - T9) and T6 (range T4 - T9) in groups LF and L, respectively. The times to two-segment regression, S2 regression, ambulation, urination and discharge were all significantly shorter in group LF than group L. These results indicate that, for outpatient inguinal herniorrhaphy, intrathecal fentanyl combined with low-dose levobupivacaine provides good quality spinal anaesthesia and minimizes the need for intra-operative analgesia. This protocol is well suited for the outpatient setting because it features rapid recovery of full motor power, sensory function and bladder function

Journée d'étude : L’usager, l’assistance et la question de la contrepartie

Journée en hommage à Robert Castel organisée par le LIRTES (UPEC) Le mardi 20 mai 2014 à Créteil Avec la participation de Colette Bec (« Usages politiques de l’assistance en démocratie ») et Christophe Trombert (« L’assistance aux pauvres valides : évolutions des contreparties »), membres du laboratoire LISE Bien que l’usager soit une catégorie relativement ancienne servant à désigner le rapport entre les personnes qui utilisent un service (logement, école, transport…) et les pouvoirs publics..