Transportability of Trauma-Focused Cognitive Behavioral Therapy : A Case Study with Adolescents in a Residential Treatment Setting

Because of the increase in the numbers of adolescents presenting in residential care, the challenge and difficulty posed to therapists in treating this age-group, and the prevalence of chronic stress and complex trauma symptoms found in this population, the research conducted was a clinical case study investigating the transportability and effectiveness of using Trauma-Focused Cognitive Behavioral Therapy (TF-CBT), a manualized treatment format, with adolescent clients in a residential treatment setting. A doctoral candidate was trained to engage each of three residential clients in 12 individual sessions of TF-CBT. The Behavior Assessment System for Children (BASC), Children’s Depression Inventory (CDI), Jesness Behavior Checklist (JBC), Revised Children’s Manifest Anxiety Scale (RCMAS), Trauma Symptom Checklist for Children (TSCC), Working Alliance Inventory (WAI), were administered at pretest, mid-way through treatment, and at posttest to assess treatment outcome. A qualitative assessment of treatment outcome comparing pretest scores and posttest scores on measures were made, and a discussion of the treatment efficacy of TF-CBT with older adolescents in residential treatment was provided. Suggestions for future research of the application of TF-CBT with youth in a residential treatment setting are offered by the researcher. An empirical study proposal was devised to demonstrate the study\u27s application to a larger sample size

Assessment of the accuracy of 3D printed teeth by various 3D printers in forensic odontology

Additive manufacturing technology has benefited many sectors, and its use in forensic sciences has opened up a variety of new opportunities for analysing and exhibiting forensic materials. However, to perform analytical procedures on 3D printed bones and teeth in forensic odontology, the metric and morphological precision of the printed replicas must first be validated. To address this, the present study was undertaken using 12 extracted human teeth that were 3D printed using five different techniques. Manual measurements and a digital mesh comparison were used to evaluate the metric precision of all samples. The findings showed that the printed replicas were accurate to within 0.5 mm of the actual teeth. It was suggested that Digital Light Processing (DLP) prints be used for potential forensic odontology applications based on measurements, digital comparison, and ease of use

Demonstration of improved sensitivity of echo interferometers to gravitational acceleration

We have developed two configurations of an echo interferometer that rely on standing wave excitation of a laser-cooled sample of rubidium atoms that measures acceleration. For a two-pulse configuration, the interferometer signal is modulated at the recoil frequency and exhibits a sinusoidal frequency chirp as a function of pulse spacing. For a three-pulse stimulated echo configuration, the signal is observed without recoil modulation and exhibits a modulation at a single frequency. The three-pulse configuration is less sensitive to effects of vibrations and magnetic field curvature leading to a longer experimental timescale. For both configurations of the atom interferometer (AI), we show that a measurement of acceleration with a statistical precision of 0.5% can be realized by analyzing the shape of the echo envelope that has a temporal duration of a few microseconds. Using the two-pulse AI, we obtain measurements of acceleration that are statistically precise to 6 parts per million (ppm) on a 25 ms timescale. Using the three-pulse AI, we obtain measurements of acceleration that are statistically precise to 0.4 ppm on a timescale of 50 ms. A further statistical enhancement is achieved by analyzing the data across the echo envelope to improve the statistical precision to 75 parts per billion (ppb). We discuss methods for reducing prominent systematic effects due to a magnetized vacuum chamber and improving the signal-to-noise ratio. Simulations of both AI configurations with a timescale of 300 ms reached in a non-magnetic vacuum chamber suggest that an optimized experiment with improved vibration isolation and atoms selected in the mF = 0 state can result in measurements of g statistically precise to 0.3 pbb for the two-pulse AI and 0.6 ppb for the three-pulse AI.Comment: 17 pages, 9 figures, 3 table

Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species

INTRODUCTION: Invertebrate communities are central to many environmental monitoring programs. In freshwater ecosystems, aquatic macroinvertebrates are collected, identified and then used to infer ecosystem condition. Yet the key step of species identification is often not taken, as it requires a high level of taxonomic expertise, which is lacking in most organizations, or species cannot be identified as they are morphologically cryptic or represent little known groups. Identifying species using DNA sequences can overcome many of these issues; with the power of next generation sequencing (NGS), using DNA sequences for routine monitoring becomes feasible. RESULTS: In this study, we test if NGS can be used to identify species from field-collected samples in an important bioindicator group, the Chironomidae. We show that Cytochrome oxidase I (COI) and Cytochrome B (CytB) sequences provide accurate DNA barcodes for chironomid species. We then develop a NGS analysis pipeline to identifying species using megablast searches of high quality sequences generated using 454 pyrosequencing against comprehensive reference libraries of Sanger-sequenced voucher specimens. We find that 454 generated COI sequences successfully identified up to 96% of species in samples, but this increased up to 99% when combined with CytB sequences. Accurate identification depends on having at least five sequences for a species; below this level species not expected in samples were detected. Incorrect incorporation of some multiplex identifiers (MID’s) used to tag samples was a likely cause, and most errors could be detected when using MID tags on forward and reverse primers. We also found a strong quantitative relationship between the number of 454 sequences and individuals showing that it may be possible to estimate the abundance of species from 454 pyrosequencing data. CONCLUSIONS: Next generation sequencing using two genes was successful for identifying chironomid species. However, when detecting species from 454 pyrosequencing data sets it was critical to include known individuals for quality control and to establish thresholds for detecting species. The NGS approach developed here can lead to routine species-level diagnostic monitoring of aquatic ecosystems

Teaching and assessing systems thinking in engineering

This paper presents research on undergraduate engineering students’ perceptions of their learning about systems thinking

Estudios sobre el uso de la melaza y otros subproductos del azúcar en nutrición de pollos.

Se estudia el uso de azúcar, panela, cachaza y melaza como fuentes de energía en dietas para pollitos y pollos asaderos. En el experimento 1 se utiliza una dieta basada en 33 por ciento de maíz, 40 por ciento de torta de soya y 2.5 por ciento de harina de pescado. La mitad y el total del maíz fueron reemplazados exitosamente con azúcar sin afectar el aumento de peso a las 6 semanas. El nivel más alto causó una pequeña reducción en la eficiencia de utilización del alimento. La panela puede reemplazar hasta la mitad del maíz, con el reemplazo total hubo marcada disminución de la eficiencia de utilización de alimento. La cachaza reemplazando la mitad o el total del maíz causó disminuciones en el crecimiento y en la eficiencia de utilización del alimento. Con 3, 6 y 12 por ciento de melaza se lograron buenas tazas de crecimiento y ligeras disminuciones en eficiencia de utilización del alimento. En el experimento 2, la adición de 8 o 16 por ciento de melaza permite tasas de crecimiento excelentes en pollos asaderos a las 8 semanas y 5 días de edad, la eficiencia de utilización de alimento se redujo ligeramente. Cuando un 16 por ciento de melaza se suministró en dietas con torta de soya como fuente principal de proteína se presentaron severos problemas de diarrea y humedad de la cama. Estos problemas fueron menos notorios cuando se usaron harina de pescado y torta de ajonjolí como fuentes principales de proteín

Myosin Va Plays a Role in Nitrergic Smooth Muscle Relaxation in Gastric Fundus and Corpora Cavernosa of Penis

The intracellular motor protein myosin Va is involved in nitrergic neurotransmission possibly by trafficking of neuronal nitric oxide synthase (nNOS) within the nerve terminals. In this study, we examined the role of myosin Va in the stomach and penis, proto-typical smooth muscle organs in which nitric oxide (NO) mediated relaxation is critical for function. We used confocal microscopy and co-immunoprecipitation of tissue from the gastric fundus (GF) and penile corpus cavernosum (CCP) to localize myosin Va with nNOS and demonstrate their molecular interaction. We utilized in vitro mechanical studies to test whether smooth muscle relaxations during nitrergic neuromuscular neurotransmission is altered in DBA (dilute, brown, non-agouti) mice which lack functional myosin Va. Myosin Va was localized in nNOS-positive nerve terminals and was co-immunoprecipitated with nNOS in both GF and CCP. In comparison to C57BL/6J wild type (WT) mice, electrical field stimulation (EFS) of precontracted smooth muscles of GF and CCP from DBA animals showed significant impairment of nitrergic relaxation. An NO donor, Sodium nitroprusside (SNP), caused comparable levels of relaxation in smooth muscles of WT and DBA mice. These normal postjunctional responses to SNP in DBA tissues suggest that impairment of smooth muscle relaxation resulted from inhibition of NO synthesis in prejunctional nerve terminals. Our results suggest that normal physiological processes of relaxation of gastric and cavernosal smooth muscles that facilitate food accommodation and penile erection, respectively, may be disrupted under conditions of myosin Va deficiency, resulting in complications like gastroparesis and erectile dysfunction

Analysis of dietary patterns and cross-sectional and longitudinal associations with hypertension, high BMI and type 2 diabetes in Peru

OBJECTIVE: To determine if specific dietary patterns are associated with risk of hypertension, type 2 diabetes mellitus (T2DM) and high BMI in four sites in Peru. DESIGN: We analysed dietary patterns from a cohort of Peruvian adults in four geographical settings using latent class analysis. Associations with prevalence and incidence of hypertension, T2DM and high BMI were assessed using Poisson regression and generalised linear models, adjusted for potential confounders. SETTING: Four sites in Peru varying in degree of urbanisation. PARTICIPANTS: Adults aged ≥35 years (n 3280). RESULTS: We identified four distinct dietary patterns corresponding to different stages of the Peruvian nutrition transition, reflected by the foods frequently consumed in each pattern. Participants consuming the 'stage 3' diet, characterised by high proportional consumption of processed foods, animal products and low consumption of vegetables, mostly consumed in the semi-urban setting, showed the highest prevalence of all health outcomes (hypertension 32·1 %; T2DM 10·7 %; high BMI 75·1 %). Those with a more traditional 'stage 1' diet characterised by potato and vegetables, mostly consumed in the rural setting, had lower prevalence of hypertension (prevalence ratio; 95 CI: 0·57; 0·43, 0·75), T2DM (0·36; 0·16, 0·86) and high BMI (0·55; 0·48, 0·63) compared with the 'stage 3' diet. Incidence of hypertension was highest among individuals consuming the 'stage 3' diet (63·75 per 1000 person-years; 95 % CI 52·40, 77·55). CONCLUSIONS: The study found more traditional diets were associated with a lower prevalence of three common chronic diseases, while prevalence of these diseases was higher with a diet high in processed foods and low in vegetables