Aerobic Training in Rats Increases Skeletal Muscle Sphingomyelinase and Serine Palmitoyltransferase Activity, While Decreasing Ceramidase Activity

Sphingolipids are important components of cell membranes that may also serve as cell signaling molecules; ceramide plays a central role in sphingolipid metabolism. The aim of this study was to examine the effect of 5 weeks of aerobic training on key enzymes and intermediates of ceramide metabolism in skeletal muscles. The experiments were carried out on rats divided into two groups: (1) sedentary and (2) trained for 5 weeks (on a treadmill). The activity of serine palmitoyltransferase (SPT), neutral and acid sphingomyelinase (nSMase and aSMase), neutral and alkaline ceramidases (nCDase and alCDase) and the content of sphingolipids was determined in three types of skeletal muscle. We also measured the fasting plasma insulin and glucose concentration for calculating HOMA-IR (homeostasis model assessment) for estimating insulin resistance. We found that the activities of aSMase and SPT increase in muscle in the trained group. These changes were followed by elevation in the content of sphinganine. The activities of both isoforms of ceramidase were reduced in muscle in the trained group. Although the activities of SPT and SMases increased and the activity of CDases decreased, the ceramide content did not change in any of the studied muscle. Although ceramide level did not change, we noticed increased insulin sensitivity in trained animals. It is concluded that training affects the activity of key enzymes of ceramide metabolism but also activates other metabolic pathways which affect ceramide metabolism in skeletal muscles

Pre- and early-postnatal nutrition modify gene and protein expressions of muscle energy metabolism markers and phospholipid fatty acid composition in a muscle type specific manner in sheep.

We previously reported that undernutrition in late fetal life reduced whole-body insulin sensitivity in adult sheep, irrespective of dietary exposure in early postnatal life. Skeletal muscle may play an important role in control of insulin action. We therefore studied a range of putative key muscle determinants of insulin signalling in two types of skeletal muscles (longissimus dorsi (LD) and biceps femoris (BF)) and in the cardiac muscle (ventriculus sinister cordis (VSC)) of sheep from the same experiment. Twin-bearing ewes were fed either 100% (NORM) or 50% (LOW) of their energy and protein requirements during the last trimester of gestation. From day-3 postpartum to 6-months of age (around puberty), twin offspring received a high-carbohydrate-high-fat (HCHF) or a moderate-conventional (CONV) diet, whereafter all males were slaughtered. Females were subsequently raised on a moderate diet and slaughtered at 2-years of age (young adults). The only long-term consequences of fetal undernutrition observed in adult offspring were lower expressions of the insulin responsive glucose transporter 4 (GLUT4) protein and peroxisome proliferator-activated receptor gamma, coactivator 1α (PGC1α) mRNA in BF, but increased PGC1α expression in VSC. Interestingly, the HCHF diet in early postnatal life was associated with somewhat paradoxically increased expressions in LD of a range of genes (but not proteins) related to glucose uptake, insulin signalling and fatty acid oxidation. Except for fatty acid oxidation genes, these changes persisted into adulthood. No persistent expression changes were observed in BF and VSC. The HCHF diet increased phospholipid ratios of n-6/n-3 polyunsaturated fatty acids in all muscles, even in adults fed identical diets for 1½ years. In conclusion, early postnatal, but not late gestation, nutrition had long-term consequences for a number of determinants of insulin action and metabolism in LD. Tissues other than muscle may account for reduced whole body insulin sensitivity in adult LOW sheep

Loneliness and social media: A qualitative investigation of young people's motivations for use and perceptions of social networking sites

The democratisation of Internet access has incrementally changed every domain of activity and has created new business and economic models. From answering work emails to learning a new language, shopping, booking medical appointments or managing one’s finances, almost everything is attainable at the click of a button. The added implications of the rapid rise of social networking websites (SNSs), such as Facebook, Twitter, Instagram or Snapchat, have further contributed to changing the way we communicate and build new friendships. Indeed most of our social relationships are now being ‘increasingly developed and maintained online’ (Nowland, Necka & Cacioppo, 2017: 1). Ostensibly, despite improved Internet access and enhanced social connectedness, modern societies are struggling to combat loneliness. It is reported to affect people of all ages, especially young adults (16-24 and 25-34 years old) who are avid Internet and social media users (see Office for National Statistics, 2018)

Wellbeing and older adults in primary health care in Poland

Introduction and objective. The Polish health service is in need of improvement because of the increasing number of geriatric patients. Identifying the resources available to patients is important for ameliorating deficits. The goal of this research was to measure the health and wellbeing of seniors and to investigate to what extent health services meet their needs. Differences in access and scope of received services between patients from different localities (i.e villages, small towns and big cities) were analysed. Materials and method. Questionnaires (Satisfaction with Life Scale; VAS Numeric Pain Distress Scale) and a structured interview assessing the quality of healthcare were carried out in 2015 on a sample of 459 seniors. Each candidate gave consent for participation in the study. Statistica 12.5 software was used for analyses. Results. The overall measure of satisfaction with life of the respondents (M=22.34 SD=5.78) was good. Inhabitants of small towns had a significantly lower sense of quality of life than seniors from big cities (Tukey HSD = 0.047, p<.05). Multimorbidity and polypharmacy were present to a limited extent. The issue of discrimination of older individuals by medical personnel affected a fifth of seniors living in villages, and one- third of those living in big cities. Geriatric patients did not have equal opportunities for access to medical services. Conclusions. State-provided geriatric care is insufficient and does not meet the real needs of patients. A holistic care approach and/or individualized care for older adults are often indicated but are only theoretical constructs in Poland. This inefficient system places the burden of caregiving on the family of the ageing patient. Anxiety among geriatric patients is growing regarding exclusion and lack of care

An evaluation of life satisfaction and health – Quality of life of senior citizens

Introduction Modern medicine is still searching for the antecedents which will lead to [i]successful aging[/i]. The article discusses the self-perception of life satisfaction and health of senior citizens. The aim of the study was to determine the relationship between self-evaluation of life satisfaction and health by senior citizens in comparison to different age groups. Material and Methods The study included 463 persons – 230 men and 233 women. The age of the participants was in the range 16 – 83 years. All participants were asked to fill the Life Satisfaction Questionnaire (Fragebogen zur Lebenszufriedenheit – FLZ). The FLZ questionnaire assesses the global life satisfaction of a person and health domain separately. Results The results show age-related differences in the evaluation of life satisfaction. Accordingly, there is a significant change in health evaluations in different age groups, but there are no significant gender differences in health self-report data. The senior citizens’ assessment of general health, although the lowest among all the age-subgroups, showed significant difference only in relation to the people below 45 years of age. The significant differences in satisfaction from mental health occurred only for the elderly and participants aged 25–34 and 35–44. Conclusions Life satisfaction is associated with subjective health evaluations. There are two domains (mental health and performance) that are positively evaluated by more than two-thirds of senior citizens. The observed differences challenge stereotypes and prejudices relating to negative aging process. Senior citizens can improve their control beliefs and develop self-regulation and coping skills

Polish adaptation of the driving and riding avoidance scale

Driving anxiety is a relatively undervalued topic of research, despite the fact that it can have a substantial detrimental impact on an individual’s life. The prevalence of driving anxiety in motor vehicle crash (MVC) survivors has been found to range from 18-77%. Although driving anxiety can develop without crash involvement, no information currently exists on the prevalence of driving anxiety in the general population. One barrier to gathering this information is that most of the instruments are designed to measure driving anxiety in MVC survivors. However, the Driving and Riding Avoidance Scale (DRAS; Stewart & St. Peter, 2004) is one instrument that shows promise as a more general measure of driving anxiety, although previous research has noted the need for some minor adaptations (Taylor & Sullman, 2009). Therefore, the present study investigated the psychometric properties of an adapted version of the DRAS and the level of driving anxiety amongst a sample of 210 Polish participants. Internal consistency for the overall DRAS was .91 and ranged from .77 to .85 for the subscales. Factor analysis of the DRAS resulted in two clear factors, with the first containing driving avoidance items and the second consisting solely of riding avoidance items. Therefore it appears that the DRAS can be a useful measure of driving avoidance in samples drawn from the general population as well as MVC survivors.falsePublishe

Chitosan–Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions

In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed. Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol–gel method (ChS1). It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m²/g, pH_PZC = 5.7 for ChS3 and 356 m²/g and pH_PZC = 6.0 for ChS2. The microporous chitosan–silica material with a specific surface area of 600 m²/g and a more negatively charged surface (pH_PZC = 4.2) was obtained by the sol–gel reaction. The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished. The generalized Langmuir equation and its special cases, that is, Langmuir–Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data. The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye]. For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations. In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min. The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime). In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)