Induced spawning in common sole (Solea solea L.)

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How Different Stocking Densities Affect Growth and Stress Status of Acipenser baerii Early Stage Larvae

In the present study, a multidisciplinary approach was used in order to evaluate growth, muscle development, and stress status in Siberian sturgeon Acipenser baerii larvae at schooling (T1) and complete yolk sac absorption (T2), reared at three stocking densities (low, medium, and high). Larvae growth, morphological muscle development, and whole-body cortisol levels were assessed. The expression of genes involved in the growth process (igf1 and igf2), in the myogenesis (myog), and in the regulation of cellular stress (glut1, glut2, glut4, and hsp70) was analyzed using a quantitative PCR. Larvae reared at lower densities showed a higher Specific Growth Rate and showed a physiological muscle development. Cortisol levels were low and did not differ significantly, both in different time sampling and across densities, suggesting that either the considered densities are not stressors in this species in the early stages of development or the hypothalamus-pituitary-adrenal (HPA) axis is not yet fully mature. Gene expression of glut1, igf1, and igf2 showed an up-regulation in both developmental stages at all the rearing densities considered, while myog significantly up-regulated at T1 at the highest density. Considering all of the results, it would seem that lower densities should be used in these stages of development, as these showed a higher growth rate, even if it is not economically feasible in commercial hatcheries. Therefore, choosing an intermediate stocking density could be a good compromise between larval performance and economical feasibility

Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens

Due to their importance for the control of meat quality in broiler chickens, the present study aimed at identifying the factors associated with the occurrence of myopathies and characterizing the meat properties when affected by myopathies. To this aim, a total of 768 broiler chickens were reared until slaughter (46 d) to evaluate the effect of genotype, gender, and feeding regime (ad libitum vs. restricted rate, 80% from 13 to 21 d of age) on performance and meat quality. Standard broilers were heavier (3,270 vs. 3,139 g; P < 0.001) and showed lower feed conversion (1.56 vs. 1.61; P < 0.001) than the high-yield broilers. Males showed higher final live weight (3,492 vs. 2,845 g) and lower feed conversion (1.54 vs. 1.63) than females (P < 0.001). Feed restriction decreased final live weight (3,194 vs. 3,142 g; P < 0.01) and feed conversion (1.60 vs. 1.57; P < 0.01) compared to ad libitum feeding. At gross examination, feed restriction tended to increase white-striped breasts (69.5 vs. 79.5%; P < 0.10), whereas females showed less wooden breasts than males (8.0 vs. 16.3%; P < 0.05). White-striped fillets had higher pHu (5.87 vs. 5.83), and lower a* (-0.81 vs. -0.59) and b* color indexes (13.7 vs. 14.5) (P < 0.05), whereas wooden breast fillets exhibited higher cooking losses (25.6 vs. 22.1%) and AK-shear force (4.23 vs. 2.84 kg/g) compared with normal fillets (P < 0.001). At histological examination, 3.1% of pectoralis major were normal, 26.6% mildly degenerated, 45.3% moderately degenerated, and 25.0% severely degenerated. In conclusion, genotype had a moderate effect on growth without modifying myopathy occurrence. In contrast, gender and feed restriction affected performance, meat quality, and breast abnormalities

Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax)

The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. From day 30 of larval life, IGF-I immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in diploids. At day 30, triploids exhibited a standard length significantly higher than the one of diploids. Although IGF-II and MSTN immunoreactivity was detectable in different tissues and organs, no differences between diploids and triploids were observed. The spatial localization of IGF-I, IGF-II and MSTN proteins detected in this study is in agreement with previous findings on the distribution of these proteins in diploid larvae and fry. The highest IGF-I immunoreactivity observed in triploids suggests a possible involvement of ploidy in their growth performance

Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasmas in grapevine

In order to devise a procedure to be used as reference for detection of grapevine phytoplasmas and monitoring of Flavescence dorée, 12 combinations comprising three methods of plant DNA extraction and 4 procedures for amplification in polymerase chain reaction of phytoplasma DNA were examined in parallel using the same plant tissues infected with phytoplasmas. In a first series tissues of periwinkles (Catharanthus roseus) infected with phytoplasma isolates of the Elm yellows group (16SrV) and maintained in the greenhouse, were used. In a second series tissues of grapevines (Vitis vinifera) naturally infected with Flavescence dorée or Palatinate grapevine yellows phytoplasma were used. The DNA preparations obtained with each of the three extraction procedures were used undiluted or serially diluted, as target DNA in the 4 nested-polymerase chain reactions. The results showed differences in the efficiency among different methods of extraction as well as in the sensitivity among the DNA amplification procedures, which improved when DNA extracted from field grapevines was diluted. After additional comparative validation on numerous field-collected samples of GY-affected grapevines, the quickest extraction procedure was selected for use in routine diagnosis, with nested-PCR amplification either of ribosomal DNA or of the FD9 DNA fragment specific for Flavescence dorée and other 16SrV group phytoplasmas

Effects of dietary barley on rainbow trout exposed to an acute stress challenge

Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.[EN] The present study evaluates the effect of dietary barley, based on its potential stress-relieving properties, on rainbow trout under acute stress challenge (hypoxia and crowding) and their recovery. Diets were formulated containing increasing barley concentrations (0, 4, 8, 16, 32%). Cortisol on plasma and fin, glucose and lactate plasma levels and malondialdehyde (MDA) in muscle were determined under normoxia before the stress test (basal levels), 30 min after the acute stress challenge and also during normoxia recovery (6 and 12 h after the stress). Results showed that at basal levels the inclusion of barley had no influence on cortisol, glucose nor on lactate values. After 30 min from the stress challenge, there was a significant increase in cortisol, glucose and lactate concentration in fish of all groups. Plasma cortisol showed the lowest levels in fish fed with diets at a medium (8%) of barley concentration and returned to basal levels 6 h after the stress stimulus with no differences between diets. Glucose values showed a less clear tendency 30 min after the stress challenge with lower levels in the control group, fish fed with 8% and 32% of barley in the diets and returned to basal levels in almost all the groups only 12 h after the stress challenge. Lactate showed the same trend as with glucose after the stress challenge but it returned to basal levels in 6 h. Interestingly, there was a significant decrease of lipid oxidation (MDA) in muscle soon after the stress test of fish fed with the highest barley levels. The present results suggest a potential positive effect of dietary barley on trout stress response.This work has been co-funded with FEDER and INIA funds. Julia Pinedo has been granted with the FPI-INIA grant number 21 (call 2012, BOE-2012-13337).Pinedo-Gil, J.; Martín-Diana, AB.; Bertotto, D.; Sanz-Calvo, M.; Jover Cerda, M.; Tomas-Vidal, A. (2019). Effects of dietary barley on rainbow trout exposed to an acute stress challenge. Aquaculture. 501:32-38. https://doi.org/10.1016/j.aquaculture.2018.10.070S323850

Geochemical variations in Cenozoic back-arc basalts at the border of La Pampa and Mendoza provinces, Argentina

Back-arc volcanism was active in central-western Argentina (provinces of Mendoza and La Pampa) from the Miocene through historic times. The rocks of 39 monogenetic volcanoes located in this area were studied in order to define their geochemical characteristics. The dominant rock texture is porphyritic, with intergranular, pilotaxitic and hyalophitic groundmasses. The most frequent phenocrysts are olivine followed by olivine–plagioclase–clinopyroxene. Their SiO2 content varies between 42.3 and 51.2 wt.%, the most abundant rocks are trachybasalts, followed by basalts and basanites, all of them alkaline. The rocks display enrichment of incompatible elements that varies according to the geographic location and age. There is an increase in incompatible element concentrations from the southern and central to the northern zones. Also, in the northern part of the study area, the behavior of incompatible elements varies with time; the incompatible element ratios of the Plio–Pleistocene rocks show arc signature, while the rocks of the Miocene De la Laguna volcano show intraplate affinity. We conclude for this sector that the mantle source region was modified after the generation of Miocene magmas by subduction-related fluids. These fluids are related to a late Miocene episode of subhorizontal subduction, i.e., after the generation of the rocks of De la Laguna volcano.Centro de Investigaciones GeológicasConsejo Nacional de Investigaciones Científicas y TécnicasUniversidad Nacional del Su

Effects of dietary inclusions of red beet and betaine on the acute stress response and muscle lipid peroxidation in rainbow trout

[EN] This study evaluates the effects of red beet (RB) and betaine on rainbow trout submitted to an acute stress challenge. A control diet was compared with four experimental diets in which red beet (14 and 28%) and betaine (0.9 and 1.63%) were incorporated in different concentrations according to a factorial design. Cortisol in plasma and fin, glucose and lactate plasma levels, and malondialdehide (MDA) in muscle were all measured before the stress challenge and 30 min and 6 and 12 h after the stress challenge as parameters to determine the diet effects. RB and betaine had no effect on cortisol, glucose, and MDA basal levels. However, lactate basal levels were significantly lower on fish fed with RB and betaine. Thirty minutes after the stress challenge, there was a significant increase in plasma and fin cortisol, glucose and lactate concentrations, although fish fed with diets containing RB and betaine showed significantly higher plasma cortisol values. MDA values of fish fed with 14% RB and 0.9% betaine were significantly higher than MDA values from fish fed with 28% RB and 1.63% betaine. After 6 and 12 h, plasma and fin cortisol and lactate levels recovered in a similar trend. Glucose plasma levels recovered in almost all groups 12 h after the stress. Also, MDA values recovered basal levels after 6 and 12 h. RB and betaine did not enhance the tolerance to the stress challenge compared to the control group, although the presence of these ingredients had no negative effect on any of the stress indicators.Pinedo-Gil, J.; Martín-Diana, AB.; Bertotto, D.; Sanz-Calvo, MÁ.; Jover Cerda, M.; Tomas-Vidal, A. (2018). Effects of dietary inclusions of red beet and betaine on the acute stress response and muscle lipid peroxidation in rainbow trout. Fish Physiology and Biochemistry. 44(3):939-948. doi:10.1007/s10695-018-0483-3S939948443Aluru N, Vijayan MM (2006) Aryl hydrocarbon receptor activation impairs cortisol response to stress in rainbow trout by disrupting the rate limiting steps in steroidogenesis. Endocrinology 147:1895–1903Ashley PJ (2007) Fish welfare: current issues in aquaculture. Appl Anim Behav Sci 104:199–235Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3–26Bertotto D, Poltronieri C, Negrato E, Majolini D, Radaelli G, Simontacchi C (2010) Alternative matrices for cortisol measurement in fish. Aquac Res 41:1261–1267Bertotto D, Poltronieri C, Negrato E, Richard J, Pascoli F, Simontacchi C, Radaelli G (2011) Whole body cortisol and expression of HSP70, IGF-I and MSTN in early development of sea bass subjected to heat shok. Gen Comp Endocrinol 174:44–50Chagas EC, Val AL (2006) Ascorbic acid reduces the effects of hypoxia on the Amazon fish tambaqui. J Fish Biol 69:608–612Cui XJ, Zhou QC, Liang HO, Yang J, Zhao LM (2010) Effects of dietary carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of juvenile cobia (Rachycentron canadum Linnaeus.) Aquac Res 42:99–107Dabrowski K, Lee KJ, Guz L, Verlhac V, Gabaudan J (2004) Effects of dietary ascorbic acid on oxygen stress (hypoxia or hyperoxia), growth and tissue vitamin concentration in juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture 233:383–392Enes P, Panserat S, Kaushik S, Oliva-Teles A (2006) Rapid metabolic adaptation of European sea beass (Dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress. Comp Biochem Physiol A 145:73–81Fast MD, Hosoya S, Johnson SC, Alfonso LOB (2008) Cortisol response and immune-related effects of Atlantic salmon (Salmo salar Linnaeus) subjected to short- and long-term stress. Fish Shellfish Immunol 24:194–204Francis G, Makkar HPS, Becker K (2001) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199:197–227Ganessan B, Anandan R, Lakshmanan PT (2011) Studies on the protective effects of betaine against oxidative damage during experimentally induced restraint stress in Wistar albino rats. Cell Stress Chaperones 16:641–652Gesto M, López-Patiño MA, Hernández J, Soengas JL, Míguez JM (2013) The response of brain serotonergic and dopaminergic systems to an acute stressor in rainbow trout: a time course study. J Exp Biol 216:4435–4442Gesto M, López-Patiño MA, Hernández J, Soengas JL, Míguez JM (2015) Gradation of the stress response in rainbow trout exposed to stressors of different severity: the role of brain serotonergic and dopaminergic systems. J Neuroendocrinol 27:131–141Hemre GI, Mommsen TP, Krogdahl Å (2002) Carbohydrates in fish nutrition: effects on growth, glucose metabolism and heptic enzymes. Aquac Nutr 8:175–194Ings JS, Vijayan MM, Servos MR (2012) Tissue-specific metabolic changes in response to an acute handling disturbance in juvenile rainbow trout exposed to municipal wastewater effluent. Aquat Toxicol 108:53–59Janssens PA, Waterman J (1988) Hormonal regulation of gluconeogenesis and glycogenolysis in carp (Cyprinus carpio) liver pieces cultured in vitro. Comp Biochem Physiol 91A:451–457Jeney G, Galeotti M, Volpatti D, Anderson DP (1997) Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture 154:1–15Kaplan LA, Pesce AJ (1984) Clinical chemistry: theory, analysis and correlation. Mosby, St. Louis, pp 1032–1036Krogdahl Å, Sundby A, Olli JJ (2004) Atlantic salmon (Salmon salar) and rainbow trout (Oncorhynchus mykiss) digest and metabolize nutrients differently. Effects of water salinity and dietary starch levels. Aquaculture 229:335–360Kujala TS, Vienola MS, Klika KD, Loponen JM, Pihlaja K (2002) Betalain and phenolic composition of four beetroot (Beta vulgaris) cultivars. Eur Food Res Technol 214:505–510Kumar N, Jadhao SB, Chandan NK, Kumar K, Jha AK, Bhushan S, Kumar S, Rana RS (2012) Dietary choline, betaine and lecithin mitigate endosulfan-induced stress in Labeo rohita fingerlings. Fish Physiol Biochem 38:989–1000Leveelahti L, Rytkönen KT, Renshaw GMC, Nikinmaa M (2014) Revisiting redox-active antioxidant defences in response to hypoxic challenge in both hypoxia-tolerant and hypoxia-sensitive fish species. Fish Physiol Biochem 40:183–191Lushchak VI, Bagnyukova TV, Lushchak OV, Storey JM, Storey KB (2005) Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio) tissues. Int J Bichem Cell Biol 37:1319–1330Lushchak VI, Bagnyukova TV (2006) Temperature increase results in oxidative stress in goldfish tissues. 1. Indices of oxidative stress. Comp Biochem Physiol C 143:30–35Madaro A, Olsen RE, Kristiansen TS, Ebbeson LOE, Nilsen TO, Flik G, Gorissen M (2015) Stress in Atlantic salmon: response to unpredictable chronic stress. J Exp Biol 218:2538–2550Ming J, Xie J, Xu P, Ge X, Liu W, Ye J (2012) Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Fish Shellfish Immunol 32:651–661Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish 9:211–268Montero D, Tort L, Robaina L, Vergara JM, Izquierdo MS (2001) Low vitamin E in diet reduces stress resistance of gilthead seabream (Sparus aurta) juveniles. Fish Shellfish Immunol 11:473–490Ortuño J, Esteban MA, Meseguer J (2003) Effect of dietary intake of vitamins C and E on the stress response of gilthead seabream (Sparus aurata L.) Fish Shellfish Immunol 14:145–156Øverli Ø, Sørensen C, Kiessling A, Pottinger TG, Gjøen HM (2006) Selection for improved stress tolerance in rainbow trout (Oncorhynchus Mykiss) leads to reduced feed waste. Aquaculture 261:776–781Pérez-Jiménez A, Peres H, Rubio VC, Oliva-Teles A (2012) The effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (Sparus aurata) fed on diets supplemented with methionine and white tea. Comp Biochem Physiol C 155:506–516Pichavant K, Maxime V, Thébault MT, Ollivier H, Garnier JP, Bousquet B, Diouris M, Boeuf G, Nonnotte G (2002) Effects of hypoxia and subsequent recovery on turbot (Scophthalmus maximus): hormonal changes and anaerobic metabolism. Mar Ecol Prog Ser 225:275–285Pinedo-Gil J, Tomás-Vidal A, Larrán-García AM, Tomás-Almenar C, Jover-Cerdá M, Sanz-Calvo MA, Martín-Diana AB (2017a) Enhancement of quality of rainbow trout (Oncorhynchus mykiss) flesh incorporating barley on diet without negative effect on rearing parameters. Aquacult Int 25:1005–1023. https://doi.org/10.1007/s10499-016-0091-0Pinedo-Gil J, Tomás-Vidal A, Jover-Cerdá M, Tomás-Almenar C, Sanz-Calvo MA, Martín-Diana AB (2017b) Red beet and betaine as ingredients in diets of rainbow trout (Oncorhynchus mykiss): effects on growth performance, nutrient retention and flesh quality. Arch Anim Nutr 71:486–505. https://doi.org/10.1080/1745039X.2017.1391503Rabeh NM (2015) Effect of red beetroot (Beta vulgaris L.) and its fresh juice against carbon tetrachloride induced hepatotoxicity in rats. World Appl Sci J 33(6):931–938Rollo A, Sulpizio R, Nardi M, Silvi S, Orpianesi C, Caggiano M, Cresci A, Carnevalli O (2006) Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiol Biochem 32:167–177Sadoul B, Leguen I, Colson V, Friggens NC, Prunet P (2015) A multivariate analysis using physiology and behaviour to characterize robustness in two isogenic lines of rainbow trout exposed to a confinement stress. Physiol Behav 140:139–147Tan Q, Xie S, Zhu X, Lei W, Yang Y (2006) Effect of dietary carbohydrates sources on growth performance and utilization for gibel carp (Carassius auratus) and Chinese longsnout catfish (Leiocassis Longirostris Günther). Aquac Nutr 12:61–70Tintos A, Míguez JM, Mancera JM, Soengas JL (2006) Development of a microtitre plate indirect ELISA for measuring cortisol in teleosts, and evaluation of stress responses in rainbow trout and gilthead sea bream. J Fish Biol 68:251–263Van Anholt RD, Spanings FAT, Koven WM, Nixon O, Wendelaar Bonga SE (2004) Arachidonic acid reduces the stress response of gilthead seabream, Sparus aurata L. J Exp Biol 207:3419–3430Virtanen E (1995) Piecing together the betaine puzzle. Feed Min 3:12–17Wu XY, Liu YJ, TIan LX, Mai KS, Yang HJ (2007) Utilization of several different carbohydrate sources by juvenile yellowfin seabream (Sparus latus). J Fish China 31(4):463–471Yoshida Y, Itoh N, Hayakawa M, Piga R, Cynshi O, Jishage K, Niki E (2005) Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE. Toxicol Appl Pharmacol 208:87–97Zeng L, Wang YH, Ai CX, Zheng JL, Wu CW, Cai R (2016) Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress. Fish Physiol Biochem 42:1395–1405Zolderdo AJ, Algera DA, Lawrence MJ, Gilmour KM, Fast MD, Thuswaldner J, Willmore WG, Cooke SJ (2016) Stress, nutrition and parental care in a teleost fish: exploring mechanisms with supplemental feeding and cortisol manipulation. J Exp Biol 219:1237–124

Effect of feed restriction timing on live performance, breast myopathy occurrence, and muscle fiber degeneration in 2 broiler chicken genetic lines

During recent years, research on meat quality in poultry has aimed to evaluate the presence and consequences of breast myopathies as well as the factors which can affect their occurrence by modifying the growth rate. A total of 900 broiler chickens were reared until slaughter (48 D) to evaluate the effect of 2 genetic lines (A vs. B) and feeding plans (ad libitum [AL], early restricted [ER], from 13 to 23 D of age, and late restricted [LR], from 27 to 37 D of age; restriction rate: 80%) on performance, meat quality, and breast muscle myopathies. Calsequestrin and vascular endothelial growth factor (VEGF) expressions, and muscle fiber degeneration (MFD) were recorded at 22, 36, and 48 D. Chickens in the AL treatment had greater final live (P < 0.01) and carcass weights and proportion of pectoralis major muscle (P = 0.04) compared to chickens in the LR treatment, whereas chickens in the ER treatment had intermediate final live (3,454 g) and carcass weights, and proportion of pectoralis major muscle (25.6%). Chickens of line A were heavier than chickens of line B (P < 0.001), and had a greater feed conversion rate. Chickens of line A also had a greater dressing out percentage (P < 0.001), but a lower proportion of pectoralis major muscle (P = 0.04), as well as a greater meat pH (P < 0.001), meat cooking losses (P < 0.01), and shear force of the pectoralis major muscle (P = 0.03). Calsequestrin and VEGF mRNA were significantly lower in ER and LR chickens compared to AL chickens after feed restriction and during refeeding (P < 0.05). MFD scores increased with chicken age (P < 0.001) and differed between genetic lines (P < 0.001). Neither feeding plan nor genetic line affected the occurrence of white striping or wooden breast condition