Computational Evolutionary Embryogeny

Evolutionary and developmental processes are used to evolve the configurations of 3-D structures in silico to achieve desired performances. Natural systems utilize the combination of both evolution and development processes to produce remarkable performance and diversity. However, this approach has not yet been applied extensively to the design of continuous 3-D load-supporting structures. Beginning with a single artificial cell containing information analogous to a DNA sequence, a structure is grown according to the rules encoded in the sequence. Each artificial cell in the structure contains the same sequence of growth and development rules, and each artificial cell is an element in a finite element mesh representing the structure of the mature individual. Rule sequences are evolved over many generations through selection and survival of individuals in a population. Modularity and symmetry are visible in nearly every natural and engineered structure. An understanding of the evolution and expression of symmetry and modularity is emerging from recent biological research. Initial evidence of these attributes is present in the phenotypes that are developed from the artificial evolution, although neither characteristic is imposed nor selected-for directly. The computational evolutionary development approach presented here shows promise for synthesizing novel configurations of high-performance systems. The approach may advance the system design to a new paradigm, where current design strategies have difficulty producing useful solutions

Genetic programming of an artificial neural network for robust control of a 2-D path following robot

Genetic Programs that have phenotypes created by the application of genotypes comprising rules are robust and highly scalable. Such encodings are useful for complex applications such as controller design. This paper outlines an evolutionary algorithm capable of creating a controller for 2 DOF, path following robot. The controllers are embodied by Artificial Neural Networks capable of full functionality despite multiple failures

Engineering by fundamental elements of evolution

The method presented in this note mimics two fundamental mechanisms from nature, growth, and development, for the synthesis of new three-dimensional structures. The structures were synthesized to support a load generated by a wind. Every structure grows from a single artificial cell following a set of genes, encoded in an artificial genome shared by all cells. Genes are a set of commands that control the growth process. Genes are regulated by interaction with the environment. The environment is both external and internal to the structure. The performance each structure is measured by its ability to hold the load and other additional engineering criteria. A population of structures is evolved using a genetic algorithm, which alters the genome of two mating individuals. We will present evolved phenotypes with high degrees of modularity and symmetry which evolved according to engineering criteria. Neither one of these two characteristics has been directly imposed as the fitness evaluation, but rather spontaneously emerge as a consequence of natural selection. We will argue that the types of rules we are using in this model are not biased toward any of these characteristics, but rather basic rules for growth and development

An investigation into the structure of genomes within an evolution that uses embryogenesis

Evolutionary algorithms that use embryogenesis in the creation of individuals have several desirable qualities. Such algorithms are able to create complex, modular designs which can scale well to large problems. However, the inner workings of developmental algorithms have not been investigated as thoroughly as their direct-encoding counterparts. More precisely, it would be beneficial to look at how the rules used during embryogenesis evolve alongside the phenotypes they produced. This paper reports on such an investigation into the evolution of a rule set for the growth of an artificial neural network, and identifies several aspects that are desirable for the genomes of a developmental evolutionary algorithm

Interatomic scattering in energy dependent photoelectron spectra of Ar clusters

Soft X-ray photoelectron spectra of Ar 2p levels of atomic argon and argon clusters are recorded over an extended range of photon energies. The Ar 2p intensity ratios between atomic argon and clusters’ surface and bulk components reveal oscillations similar to photoelectron extended X-ray absorption fine structure signal (PEXAFS). We demonstrate here that this technique allows us to analyze separately the PEXAFS signals from surface and bulk sites of free-standing, neutral clusters, revealing a bond contraction at the surface

Spatial structure of the 8200 cal yr BP event in northern Europe

International audienceA synthesis of well-dated high-resolution pollen records suggests a spatial structure in the 8200 cal yr BP event in northern Europe. The temperate, thermophilous tree taxa, especially Corylus, Ulmus, and Alnus, decline abruptly between 8300 and 8000 cal yr BP at most sites located south of 61° N, whereas there is no clear change in pollen values at the sites located in the North-European tree-line region. Pollen-based quantitative temperature reconstructions and several other, independent palaeoclimate proxies, such as lacustrine oxygen-isotope records, reflect the same pattern, with no detectable cooling in the sub-arctic region. The observed patterns challenges the general view of the wide-spread occurrence of the 8200 cal yr BP event in the North Atlantic region. An alternative explanation is that the cooling during the 8200 cal yr BP event took place mostly during the winter and spring, and the ecosystems in the south responded sensitively to the cooling during the onset of the growing season. In contrast, in the sub-arctic area, where the vegetation was still dormant and lakes ice-covered, the cold event is not reflected in pollen-based or lake-sediment-based records

Bim and Bmf synergize to induce apoptosis in Neisseria gonorrhoeae infection

Abstract: Bcl-2 family proteins including the pro-apoptotic BH3-only proteins are central regulators of apoptotic cell death. Here we show by a focused siRNA miniscreen that the synergistic action of the BH3-only proteins Bim and Bmf is required for apoptosis induced by infection with Neisseria gonorrhoeae (Ngo). While Bim and Bmf were associated with the cytoskeleton of healthy cells, they both were released upon Ngo infection. Loss of Bim and Bmf from the cytoskeleton fraction required the activation of Jun-N-terminal kinase-1 (JNK-1), which in turn depended on Rac-1. Depletion and inhibition of Rac-1, JNK-1, Bim, or Bmf prevented the activation of Bak and Bax and the subsequent activation of caspases. Apoptosis could be reconstituted in Bim-depleted and Bmf-depleted cells by additional silencing of antiapoptotic Mcl-1 and Bcl-XL, respectively. Our data indicate a synergistic role for both cytoskeletal-associated BH3-only proteins, Bim, and Bmf, in an apoptotic pathway leading to the clearance of Ngo-infected cells. Author Summary: A variety of physiological death signals, as well as pathological insults, trigger apoptosis, a genetically programmed form of cell death. Pathogens often induce host cell apoptosis to establish a successful infection. Neisseria gonorrhoeae (Ngo), the etiological agent of the sexually transmitted disease gonorrhoea, is a highly adapted obligate human-specific pathogen and has been shown to induce apoptosis in infected cells. Here we unveil the molecular mechanisms leading to apoptosis of infected cells. We show that Ngo-mediated apoptosis requires a special subset of proapoptotic proteins from the group of BH3-only proteins. BH3-only proteins act as stress sensors to translate toxic environmental signals to the initiation of apoptosis. In a siRNA-based miniscreen, we found Bim and Bmf, BH3-only proteins associated with the cytoskeleton, necessary to induce host cell apoptosis upon infection. Bim and Bmf inactivated different inhibitors of apoptosis and thereby induced cell death in response to infection. Our data unveil a novel pathway of infection-induced apoptosis that enhances our understanding of the mechanism by which BH3-only proteins control apoptotic cell death

Bcl-2 protein family: Implications in vascular apoptosis and atherosclerosis

Apoptosis has been recognized as a central component in the pathogenesis of atherosclerosis, in addition to the other human pathologies such as cancer and diabetes. The pathophysiology of atherosclerosis is complex, involving both apoptosis and proliferation at different phases of its progression. Oxidative modification of lipids and inflammation differentially regulate the apoptotic and proliferative responses of vascular cells during progression of the atherosclerotic lesion. Bcl-2 proteins act as the major regulators of extrinsic and intrinsic apoptosis signalling pathways and more recently it has become evident that they mediate the apoptotic response of vascular cells in response to oxidation and inflammation either in a provocative or an inhibitory mode of action. Here we address Bcl-2 proteins as major therapeutic targets for the treatment of atherosclerosis and underscore the need for the novel preventive and therapeutic interventions against atherosclerosis, which should be designed in the light of molecular mechanisms regulating apoptosis of vascular cells in atherosclerotic lesions