Dental Treatment under General Anesthesia in Healthy and Medically Compromised/Developmentally Disabled Children: A Comparative Study

Aim: To compare the type, number of procedures and working time of dental treatment provided under dental general anesthesia (DGA) in healthy and medically compromised/developmentally disabled children (MCDD children). Design: This cross-sectional prospective study involved 80 children divided into two groups of 40 children each. Group 1 consisted of healthy and Group 2 consisted of MCDD children. Results: Healthy children needed more working time than MCDD children, the means being 161±7.9 and 84±5.7 minutes, respectively (P= 0.0001). Operative dentistry and endodontic treatments showed a significant statistical difference (P= 0.0001). The means of procedures were 17±5.0 for healthy children and 11±4.8 for MCDD children (P= 0.0001). Conclusions: Healthy children needed more extensive dental treatment than MCDD children under DGA. The information from this sample of Mexican children could be used as reference for determining trends both within a facility as well as in comparing facilities in cross-population studies

Comparison of thermistor linearization techniques for accurate temperature measurement in phase change materials

Alternate energy technologies are developing rapidly in the recent years. A significant part of this trend is the development of different phase change materials (PCMs). Proper utilization of PCMs requires accurate thermal characterization. There are several methodologies used in this field. This paper stresses the importance of accurate temperature measurements during the implementation of T-history method. Since the temperature sensor size is also important thermistors have been selected as the sensing modality. Two thermistor linearization techniques, one based on Wheatstone bridge and the other based on simple serial-parallel resistor connection, are compared in terms of achievable temperature accuracy through consideration of both, nonlinearity and self-heating errors. Proper calibration was performed before T-history measurement of RT21 (RUBITHERM® GmbH) PCM. Measurement results suggest that the utilization of serial-parallel resistor connection gives better accuracy (less than ±0.1°C) in comparison with the Wheatstone bridge based configuration (up to ±1.5°C)

Recurrent Syncope, a Clue in Amyloid Cardiomyopathy

Infiltrative cardiomyopathies include a variety of disorders that lead to myocardial thickening resulting in a constellation of clinical manifestations and eventually heart failure that could be the first clue to reach the diagnosis. Among the more described infiltrative diseases of the heart is amyloid cardiomyopathy. The disease usually presents with subtle, nonspecific symptoms. Herein, we illustrate a case of recurrent syncope as the initial presenting symptom for systemic amyloid with polyneuropathy and cardiomyopathy as a cause of syncope. The article illustrates the role of advanced cardiac imaging in the diagnosis of the disease with a focused literature review. We also highlight the role of early, shared decision-making between patient, family, and medical team in the management of cardiac amyloidosis

Relationship Between the Quorum Network (Sensing/Quenching) and Clinical Features of Pneumonia and Bacteraemia Caused by A. baumannii

Acinetobacter baumannii (Ab) is one of the most important pathogens associated with nosocomial infections, especially pneumonia. Interest in the Quorum network, i.e., Quorum Sensing (QS)/Quorum Quenching (QQ), in this pathogen has grown in recent years. The Quorum network plays an important role in regulating diverse virulence factors such as surface motility and bacterial competition through the type VI secretion system (T6SS), which is associated with bacterial invasiveness. In the present study, we investigated 30 clinical strains of A. baumannii isolated in the “II Spanish Study of A. baumannii GEIH-REIPI 2000-2010” (Genbank Umbrella Bioproject PRJNA422585), a multicentre study describing the relationship between the Quorum network in A. baumannii and the development of pneumonia and associated bacteraemia. Expression of the aidA gene (encoding the AidA protein, QQ enzyme) was lower (P < 0.001) in strains of A. baumannii isolated from patients with bacteraemic pneumonia than in strains isolated from patients with non-bacteraemic pneumonia. Moreover, aidA expression in the first type of strain was not regulated in the presence of environmental stress factors such as the 3-oxo-C12-HSL molecule (substrate of AidA protein, QQ activation) or H2O2 (inhibitor of AidA protein, QS activation). However, in the A. baumannii strains isolated from patients with non-bacteraemic pneumonia, aidA gene expression was regulated by stressors such as 3-oxo-C12-HSL and H2O2. In an in vivo Galleria mellonella model of A. baumannii infection, the A. baumannii ATCC 17978 strain was associated with higher mortality (100% at 24 h) than the mutant, abaI-deficient, strain (carrying a synthetase enzyme of Acyl homoserine lactone molecules) (70% at 24 h). These data suggest that the QS (abaR and abaI genes)/QQ (aidA gene) network affects the development of secondary bacteraemia in pneumonia patients and also the virulence of A. baumannii.National Plan for Scientific ResearchTechnological Development and Innovation PI16/01163ISCIII-Deputy General Directorate for Evaluation and Promotion of Research-European Regional Development Fund A way of Making EuropeInstituto de Salud Carlos IIIMiguel Servet Research Programme SERGAS and ISCIIIXunta de Galicia (GAIN, Axencia de Innovación

Practical and accurate calculations of Askaryan radiation

An in-depth characterization of coherent radio Cherenkov pulses from particle showers in dense dielectric media, referred to as the Askaryan effect, is presented. The time-domain calculation developed in this article is based on a form factor to account for the lateral dimensions of the shower. It is computationally efficient and able to reproduce the results of detailed particle shower simulations with high fidelity in most regions of practical interest including Fresnel effects due to the longitudinal development of the shower. In addition, an intuitive interpretation of the characteristics of the Askaryan pulse is provided. We expect our approach to benefit the analysis of radio pulses in experiments exploiting the radio technique.Comment: Replaced with version published Phys. Rev.

Improved measurement technique for the characterization of organic and inorganic phase change materials using the T-history method

In the past decade, the interest in phase change materials (PCMs) has grown significantly due to their ability to store large amounts of thermal energy in relatively small temperature intervals. Accurate knowledge of thermo-physical properties is a prerequisite for any reliable utilization of these materials. The T-history method is widely used for the investigation of PCM. This paper presents an improved measurement technique for the characterization of PCM using the T-history method. The suggested improvements include the arrangements made in three different prospects: the experimental setup, data processing and data representation. T-history measurements of organic RT21 and inorganic SP22 A17 (RUBITHERM® GmbH) PCM were performed. The applied arrangements resulted in the temperature accuracy of ±0.3 °C and the reduction of uncertainty associated with heat stored/released between the cooling and heating measurements. The obtained results showed some important aspects of the T-history PCM investigation and could provide more effective design and development process of the thermal energy storage systems based on the investigated materials

Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis

Liver fibrosis, a common hallmark of chronic liver disease (CLD), is characterized by the accumulation of extracellular matrix secreted by activated hepatic fibroblasts and stellate cells (HSC). Fibrogenesis involves multiple cellular and molecular processes and is intimately linked to chronic hepatic inflammation. Importantly, it has been shown to promote the loss of liver function and liver carcinogenesis. No effective therapies for liver fibrosis are currently available. We examined the anti-fibrogenic potential of a new drug (CM414) that simultaneously inhibits histone deacetylases (HDACs), more precisely HDAC1, 2, and 3 (Class I) and HDAC6 (Class II) and stimulates the cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) pathway activity through phosphodiesterase 5 (PDE5) inhibition, two mechanisms independently involved in liver fibrosis. To this end, we treated Mdr2-KO mice, a clinically relevant model of liver inflammation and fibrosis, with our dual HDAC/PDE5 inhibitor CM414. We observed a decrease in the expression of fibrogenic markers and collagen deposition, together with a marked reduction in inflammation. No signs of hepatic or systemic toxicity were recorded. Mechanistic studies in cultured human HSC and cholangiocytes (LX2 and H69 cell lines, respectively) demonstrated that CM414 inhibited pro-fibrogenic and inflammatory responses, including those triggered by transforming growth factor β (TGFβ). Our study supports the notion that simultaneous targeting of pro-inflammatory and fibrogenic mechanisms controlled by HDACs and PDE5 with a single molecule, such as CM414, can be a new disease-modifying strateg

Live Imaging at the Onset of Cortical Neurogenesis Reveals Differential Appearance of the Neuronal Phenotype in Apical versus Basal Progenitor Progeny

The neurons of the mammalian brain are generated by progenitors dividing either at the apical surface of the ventricular zone (neuroepithelial and radial glial cells, collectively referred to as apical progenitors) or at its basal side (basal progenitors, also called intermediate progenitors). For apical progenitors, the orientation of the cleavage plane relative to their apical-basal axis is thought to be of critical importance for the fate of the daughter cells. For basal progenitors, the relationship between cell polarity, cleavage plane orientation and the fate of daughter cells is unknown. Here, we have investigated these issues at the very onset of cortical neurogenesis. To directly observe the generation of neurons from apical and basal progenitors, we established a novel transgenic mouse line in which membrane GFP is expressed from the beta-III-tubulin promoter, an early pan-neuronal marker, and crossed this line with a previously described knock-in line in which nuclear GFP is expressed from the Tis21 promoter, a pan-neurogenic progenitor marker. Mitotic Tis21-positive basal progenitors nearly always divided symmetrically, generating two neurons, but, in contrast to symmetrically dividing apical progenitors, lacked apical-basal polarity and showed a nearly randomized cleavage plane orientation. Moreover, the appearance of beta-III-tubulin–driven GFP fluorescence in basal progenitor-derived neurons, in contrast to that in apical progenitor-derived neurons, was so rapid that it suggested the initiation of the neuronal phenotype already in the progenitor. Our observations imply that (i) the loss of apical-basal polarity restricts neuronal progenitors to the symmetric mode of cell division, and that (ii) basal progenitors initiate the expression of neuronal phenotype already before mitosis, in contrast to apical progenitors

Effective Phone Calls

“Effective Phone Calls” is a practical and accessible course specifically designed to develop students` essential communication and language skills. It is divided into six units, which contain face-to-face with teacher and self-study materials. The course aims to develop both competence and confi-dence in a variety of situations, so that by the end of the period of study students will have acquired the necessary skills to handle almost any kind of call in business setting