Understanding Mn-nodule distribution and evaluation of related deep-sea mining impacts using AUV-based hydroacoustic and optical data

In this study ship- and AUV-based multibeam data from the German Mn-nodule license area in the Clarion-Clipperton Zone (CCZ; eastern Pacific) are linked to ground truth data from optical imaging. Photographs obtained by an AUV enable semi-quantitative assessments of nodule coverage at a spatial resolution in the range of meters. Together with high resolution AUV bathymetry this revealed a correlation of small-scale terrain variations ( 1.8° and concave terrain. On a more regional scale, factors such as the geological setting (existence of horst and graben structures, sediment thickness, outcropping basement) and influence of bottom currents seem to play an essential role for the spatial variation of nodule abundance and the related hard substrate habitat. AUV imagery was also successfully employed to map the distribution of re-settled sediment following a disturbance and sediment cloud generation during a sampling deployment of an Epibenthic Sledge. Data from before and after the "disturbance" allows a direct assessment of the impact. Automated image processing analyzed the nodule coverage at the seafloor, revealing nodule blanketing by resettling of suspended sediment within 16 hours after the disturbance. The visually detectable impact was spatially limited to a maximum of 100m distance from the disturbance track, downstream of the bottom water current. A correlation with high resolution AUV bathymetry reveals that the blanketing pattern varies in extent by tens of meters, strictly following the bathymetry, even in areas of only slightly undulating seafloor (< 1 m vertical change). These results highlight the importance of detailed terrain knowledge when engaging in resource assessment studies for nodule abundance estimates and defining minable areas. At the same time, it shows the importance of high resolution mapping for detailed benthic habitat studies that show a heterogeneity at scales of 10 m to 100 m. Terrain knowledge is also needed to determine the scale of the impact by seafloor sediment blanketing during mining-operations

Mineralogical investigation and preliminary beneficiation tests for upgrading the Hudenisht nickeliferous lateritic ore

The nickeliferous iron ore of Hudenisht is located in the Pogradec area, Albania. It is developed in layers above altered ophiolite and is covered by limestone of the Upper Cretaceous. The ore was characterized through XRD, ore microscopy and SEM analysis and found that the main mineral phases are hematite, goethite and chromite, with chlorite (clinochlore, nimite), lizardite, calcite and spinel as minor phases. The main Ni-bearing phase is nimite, which appears mainly in the groundmass of the ore. The chemical analysis through XRF showed that it is a lowgrade nickel ore (about 0.8% NiO) with Fe2O3 (74%) as the most abundant constituent. The ore was upgraded through physical beneficiation processes, namely gravimetric and magnetic separations. The received samples were homogenized, crushed to -8 mm and the product was sieved following the wet sieving procedure to obtain four size fractions, i.e. −8+4 mm, −4+1 mm, −1+0.250 mm and −0.250+0.063 mm. Each fraction was subjected to upgrading and the chemical analysis through XRF showed that the best results among the two processes were obtained through the gravimetric separation. It is observed that the float products are richer in nickel mainly in finer size fractions

The Majority of MicroRNAs Detectable in Serum and Saliva Is Concentrated in Exosomes

There is an increasing interest in using microRNAs (miRNA) as biomarkers in autoimmune diseases. They are easily accessible in many body fluids but it is controversial if they are circulating freely or are encapsulated in microvesicles, particularly exosomes. We investigated if the majority of miRNas in serum and saliva are free-circulating or concentrated in exosomes. Exosomes were isolated by ultracentrifugation from fresh and frozen human serum and saliva. The amount of selected miRNAs extracted from the exosomal pellet and the exosome-depleted serum and saliva was compared by quantitative RT-PCR. Some miRNAs tested are ubiquitously expressed, others were previously reported as biomarkers. We included miRNAs previously reported to be free circulating and some thought to be exosome specific. The purity of exosome fraction was confirmed by electronmicroscopy and western blot. The concentration of miRNAs was consistently higher in the exosome pellet compared to the exosome-depleted supernatant. We obtained the same results using an equal volume or equal amount of total RNA as input of the RT-qPCR. The concentration of miRNA in whole, unfractionated serum, was between the exosomal pellet and the exosome-depleted supernatant. Selected miRNAs, which were detectable in exosomes, were undetectable in whole serum and the exosome-depleted supernantant. Exosome isolation improves the sensitivity of miRNA amplification from human biologic fluids. Exosomal miRNA should be the starting point for early biomarker studies to reduce the probability of false negative results involving low abundance miRNAs that may be missed by using unfractionated serum or saliva

Increasing maritime situation awareness via trajectory detection, enrichment and recognition of events

The research presented in this paper aims to show the deployment and use of advanced technologies towards processing surveillance data for the detection of events, contributing to maritime situation awareness via trajectories’ detection, synopses generation and semantic enrichment of trajectories. We first introduce the context of the maritime domain and then the main principles of the big data architecture developed so far within the European funded H2020 datAcron project. From the integration of large maritime trajectory datasets, to the generation of synopses and the detection of events, the main functions of the datAcron architecture are developed and discussed. The potential for detection and forecasting of complex events at sea is illustrated by preliminary experimental results