19 research outputs found
Peer Feedback in Japanese EFL University Classes : Perspectives from Native English Instructors
departmental bulletin pape
Cancer Nursing Research on the learning needs of hospital nurses FY2010
当院が都道府県がん診療連携拠点病院に指定されてから、がん患者の受診が多くなり現在では約4割ががん患者である。がん医療に携わる看護職の人材育成のために勉強会や事例検討会を行ってきた。しかし年々参加人数が減少し、プログラム自体の見直しが必要であると思われ、看護師の学習ニーズを明らかにしたいと考えた。そこでアンケート調査を行ったところ、がん看護への関心が高いことが明らかになり、対象者を絞った学習計画を立てることが必要と示唆された。Article信州大学医学部附属病院看護研究集録 39(1): 5-18(2011)departmental bulletin pape
HMG Domain Containing SSRP1 Is Required for DNA Demethylation and Genomic Imprinting in Arabidopsis
In Arabidopsis, DEMETER (DME) DNA demethylase contributes to reprogramming of the epigenetic state of the genome in the central cell. However, other aspects of the active DNA demethylation processes remain elusive. Here we show that Arabidopsis SSRP1, known as an HMG domain-containing component of FACT histone chaperone, is required for DNA demethylation and for activation and repression of many parentally imprinted genes in the central cell. Although loss of DNA methylation releases silencing of the imprinted FWA-GFP, double ssrp1-3;met1-3 mutants surprisingly showed limited activation of maternal FWA-GFP in the central cell, and only became fully active after several nuclear divisions in the endosperm. This behavior was in contrast to the dme-1;met1 double mutant in which hypomethylation of FWA-GFP by met1 suppressed the DNA demethylation defect of dme-1. We propose that active DNA demethylation by DME requires SSRP1 function through a distinctly different process from direct DNA methylation control.journal articl
Analysis of osteoclastogenesis from CaP patients' PBMCs.
<p>TRAP positive multinucleated cells were identified as OCs and counted, in both patients and healthy controls cultures, (A). The OC number in bone metastatic patients was significantly higher than in non-bone metastatic patients, <i>p</i><0.004 and in healthy controls, <i>p</i><0.001 (B).</p
IL-7 expression by CaP.
<p>IL-7 serum levels in patients with/without bone metastases and in healthy controls were measured by ELISA. Bone metastatic (<i>p</i><0.01) and non-bone metastatic patients (<i>p</i><0.03) had significantly higher IL-7 serum levels compared to healthy controls (A). CaP and healthy tissues were analyzed by Real-Time PCR in order to quantify IL-7 gene expression. The IL-7 quantization was expressed as IL-7 on β-Actin (the control gene) plasmid copy number. The histogram showed comparable IL-7 expression levels in CaP and healthy tissues.</p
DKK-1 expression is higher in CaP patients.
<p>DKK-1 levels were dosed in serum patients with/without bone metastases and in healthy controls by ELISA. Bone metastatic (<i>p</i><0.004) and non-bone metastatic patients (<i>p</i><0.01) had significantly higher DKK-1 serum levels compared to healthy controls (A). CaP and healthy tissues were analyzed by Real-Time PCR in order to quantify DKK-1gene expression. The DKK-1 quantization was expressed as DKK-1 on β-Actin (the control gene) plasmid copy number. The histogram showed higher DKK-1 expression levels in CaP than in healthy tissues, <i>p</i><0.001 (B).</p
