UJI KETAHANAN ENAM VARIETAS PADI (Oryza sativa L.) TERHADAP KEKERINGAN SECARA MORFOLOGI DAN MOLEKULER

Rice (Oryza sativa L.) is a staple crop that is always needed throughout the world, including Asia. Rice is one of the staple foods in Indonesia. One form of abiotic stress that becomes the main obstacle in rice production is drought stress. It is necessary to test rice varieties that are resistant to drought stress. This study aims to determine drought-resistant rice varieties based on SSR markers and to determine the morphological characteristics of drought-resistant rice. This research was carried out from January to April 2021 at the Laboratory of Physiology and Biotechnology, Faculty of Agriculture, Sultan Ageng Tirtayasa University and the Screen House of the Agricultural Technology Research Center Banten. The DNA isolation process in the molecular resistance test used the Doyle & Doyle (1990) method which used three SSR markers to detect drought resistance genes. Analysis of DNA bands using Gel Analyzer software, grouping analysis using the UPGMA method in NTSYS software. The results of the morphological study of six rice varieties on drought showed significant to very significant effects on the parameters of plant height (cm), root length (cm), number of tillers, and number of panicles. Molecular test results from three SSR primers on six rice varieties showed a high level of polymorphism with an average polymorphism information content (PIC) of 0.72 which was included in the very informative category and could be researched on drought resistance. The genetic similarity identified Jawara Hawara rice variety have drought resistance genes

Growth Response Of Cavendish Banana Planlets cv. grand naine At The Acclimatization Phase With Different Doses Of Foliar Fertilizer And Vitamin B1

This research aimed to determine the effect of foliar fertilizer and vitamin B1 on the growth of cavendish banana plantlets at the acclimatization phase. This research was an experimental research conducted from July to October 2023 in the Greenhouse of the Agroecotechnology Department, Faculty of Agriculture, Sultan Ageng Tirtayasa University, Serang, Banten. This research used a Factorial Randomized Block Design (RCBD) which consisted of two factors. The first factor was the foliar fertilizer dosage consist of 5 levels, namely 0 g/l (N0), 1 g/l (N1), 1.5 g/l (N2), 2 g/l (N3) and 2.5 g/l (N3). l (N4). The second factor was the vitamin B1 dosage  which consists of  3 levels, namely 1 ml/l (M1), 1.5 ml/l (M2) and 2 ml/l (M3). Each treatment was repeated three times to obtain 45 experimental units. The results showed that the foliar fertilizer dosage treatment had an effect on the parameters of plant height (2.79 cm), stem diameter (3.03 mm), number of leaves (2.33 leaves), leaf length (1.50 cm), leaf width (0 .90 cm), chlorophyll content (28.08 units). Meanwhile, the vitamin B1 dose treatment affected the parameters of stem diameter (2.20 mm), number of leaves (2 leaves), leaf length (1.44 cm) and leaf width (0.90 cm). There is an interaction between giving a dose of 2 g/l foliar fertilizer and 2 ml/l vitamin B1 on stem diameter  5 WAP (1.57 mm) and number of leaves  6 and 7 WAP (2 and 3 leaves)

DAYA REGENERASI PLBs ANGGREK DENDROBIUM VAR. JACQUELINE THOMAS x WALTER OUMAE DAN KUMALA AGRIHORTI PADA JENIS MEDIA KULTUR IN VITRO DENGAN PENAMBAHAN AIR KELAPA

Dendrobium is the epiphytic orchid and high demand because of its long-lasting freshness, color variants, shape, and high productivity. The addition of organic complex Coconut Water (CW) to the in vitro media such as MS and VW because in general organic complex compounds are sources of sugars, vitamins, growth regulators, and amino acids. The purpose of this study was to obtain the best media for plantlet regeneration from PLBs of Dendrobium orchids var. JTWO and var. Kumala Agrihorti. This study used a Completely Randomized Design consisting of two factors with five replications. The combination of  planting media with the addition of coconut water was consist of 10 levels, ½ MS+0 ml/l CW, ½ MS+75 ml/l CW, ½ MS+100 ml/l CW, ½ MS+125 ml/l CW, ½ MS+150 ml/l CW, VW+0 ml/l CW, VW+75 ml/l CW, VW+100 ml/l CW, VW+125 ml/l CW and VW+150 ml/l CW. The results showed that the variety treatment gave a significant effect on the percentage of PLBs regeneration, the number of shoots, and the increase in plantlet height. The treatment of media gave a significant effect on the percentage of PLBs regeneration, percentage of PLBs life, the number of leaves, and the number of roots. The interaction between treatments significantly affects the percentage of PLBs regeneration

Callus Induction of Ciangir Passion Fruit (Passiflora sp.) Leaves at Several Concentration Levels of Growth Regulators Substance 2,4-D and BAP

Passion fruit plants are important economically due to the high nutrient value of the fruit, and they have an important role in the pharmaceutical world. People often exploit passion fruit plants for medicinal purposes without cultivating them. Passion fruit propagation can be done using seeds, grafting, or cuttings. However, such propagation can increase the value of high genetic variability so that to overcome this can be done by propagating passion fruit seedlings in tissue culture by callus induction. This research was carried out to determine the effect of 2,4-D and BAP on callus induction in passion fruit leaves. The method utilized was a factorial Randomized Group Design (RGD), which consisted of two factors, namely the 2,4-D concentration level (0, 1, 2, 3) ppm and the BAP concentration level (0, 1, 2) ppm, so there were 12 treatment combinations which were repeated three times. The observed parameters were callus area, percentage of browning explants, percentage of living explants, percentage of callus explants, color and texture of callus. The concentration level of 2,4-D 3 ppm and BAP 0 ppm best affected the callus area parameter of 1.96 . The results of this research showed that the percentage of the highest living explants achieved 100%, the percentage of contaminated explants was 0%, the percentage of explants that experienced browning was 66%, and the percentage of explants that experienced callus was 100%, yellowish green in color of callus, and callus texture is crumbly, compact, intermediate. The research should focus on callus induction with other growth regulators

MAKNA SIMBOLIK PROSESI RITUAL TARI TAYUB PADA HARI JADI KOTA TUBAN

Penelitian ini bertujuan untuk mendeskripsikan makna simbolik prosesi ritual tari tayub pada hari jadi kota Tuban dan mendeskripsikan prosesi dan isi ritual siraman penari tayub dalam acara hari jadi kota Tuban. Penelitian menggunakan pendekatan kualitatif. Objek penelitian ini adalah ritual siraman penari tayub. Penelitian difokuskan pada makna simbolik prosesi ritual siraman penari tayub yang menjadi agenda rutin setiap tahun dalam memperingati hari jadi kota Tuban. Subjek penelitian diperoleh dari nara sumber yaitu, Juru Kunci Sendang Bektiharjo sebagai pawang, Penari Tayub, dan Pramugari tayub. Metode pengumpulan data dilakukan melalui observasi, wawancara, dan dokumentasi. Data-data dianalisis melalui tahap-tahap: reduksi data, display data, penarikan kesimpulan dan verifikasi data. Pengabsahan data dilakukan dengan triangulasi. Hasil penelitian menunjukkan bahwa: (1) makna simbolik ritual siraman penari tayub pada hari jadi kota Tuban. (a) makna simbolik kirab yaitu untuk mengungkapkan rasa syukur kepada Tuhan Yang Maha Esa, (b) makna simbolik kenduri yaitu agar diberi keselamatan dan kelancaran , (c) makna simbolik ritual siraman yaitu sebagai pembersihan diri dari segala kotoran, (d) makna simbolik berjalan mengelilingi sendang dengan membawa kendil dan menabur bunga yaitu untuk memperbanyak sumber air dan menjadikan sumber air menjadi jernih, (e) makna simbolik meminum dan mencuci muka menggunakan air dari sumur tirto wening yaitu bisa menambah awet muda dan menambah aura kecantikan, (f) makna simbolik pendanyangan yaitu untuk mengingat Tuhan Yang Maha Esa dan untuk menghormati penunggu sendang, (g) makna simbolik pementasan tayub semalam suntuk yaitu sebagai rasa syukur atas kelancaran acara ritual siraman. (2) jalannya prosesi ritual siraman penari tayub. (a) kirab, (b) kenduri, (c) ritual siraman, (d) berjalan mengelilingi sendang dengan membawa kendil dan menabur bunga, (e) meminum dan mencuci muka menggunakan air dari sumur tirto wening, (f) pendanyangan, (g) pementasan tayub semalam suntuk

MULTIPLIKASI TUNAS PADA DUA VARIETAS PISANG (Musa acuminata L.) DENGAN PEMBERIAN BEBERAPA KONSENTRASI SITOKININ

This study aimed to investigate how the application of various cytokinin concentrations affects the shoot multiplication in two varieties of banana (Musa acuminata L.). The research was conducted at the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sultan Ageng Tirtayasa University. The study was designed as a randomized complete block design, consisting of two factors. The first factor included two types of banana varieties: bagja (v1) and cavendish (v2). The second factor included six types of application of various concentrations of cytokinin, which were 1 mg/l BAP (k1), 2 mg/l BAP (k2), 3 mg/l BAP (k3), 1 mg/l kinetin (k4), 2 mg/l kinetin (k5), and 3 mg/l kinetin (k6). The results showed a significant difference in the effect of cavendish banana varieties explant on the height of the plant, with an average value of 3.26 cm. The application of various concentrations of cytokinin didn’t significantly affect the parameters of the height of the plant, shoot emergence time, number of shoots, and time of root emergence.Keywords: Bagja, Cavendish, Cytokinin, In Vitro Cultur

EVALUASI KESESUAIAN LAHAN DAN POTENSI PRODUKSI TANAMAN MANGGIS (Garcinia mangostana L.) DI KAB.50 KOTA

This research discusses the land suitability of mangosteen field in Kab.50 Kota Kota Payakumbuh, predict production potential of mangosteen based on the climate suitability, soil and management its field.  Composite soil sample is taken through drilling 0 – 60 cm which is carried out on research site.  Quantitative model from FAO (1976) is used to evaluate the land suitability.  This model combines enviromental data, climate and soil condition (soil physic and chemistry characteristic), so that production potential of field based on climate (Climate Production Potential = CPP) can be maesured quantitatively.  By entering the real data of plant production, this model can predict the real plant production in a field (Land Production Potential = LPP).  Result of this research shows that climate and physical condition of land are very suitability to grow mangosteen, and its level appropriateness is S1 (very suitability).  Then, production potential 3 – 12 ton/ha/year can be increased to > 15 ton/ha/year if the management of land is improved.Key Words :  Land suitability evaluation, Climatic Production Potential, Land  Production Potentia

HOMOLOGI FUNGSI GEN KNAT1 ( Knotted 1– like Arabidopsis thaliana) PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Bl. DENGAN MEDIATOR Agrobacterium tumefaciens

ABSTRACTTo understand the function of KNAT1 gene Arabidopsis in the forming and developing of Indonesian origin orchid shoot Phalaenopsis amabilis through transform p35S::KNAT1 and pGreen to protocorm like bodies (plb) of the orchid mediated by Agrobacterium tumafaciens LBA4404. The plb transformants were grown on New Phalaenopsis selection medium containing 5 mg/l BAP, 0.15 mg/l NAA, 15 mg.l Kanamycin and addition of 300 mg/l Cefotaxim to eliminate the overgrowth of Agrobacterium. The analysis of positive transformant use Polimerase Chain Reaction (PCR) with the specific oligonucleotide primers for KNAT1 gene: KNAT1F1R1 and universal primers for pGreen: 35SO and Tnos. The result shows that 3 shoots of 1850 transformants positively carry out the 35S::KNAT1 construct (frequency of transformation was 0.16 %) while 5 shoot of 1850 transformants also positively carry out the pGreen vector, with the frequency of transformation was 0.27 %. The phenotype analysis of 35S::KNAT1 transformants show multiplication on forming of the leaf from a plb to + 10 shoots and forming of the leaf shape which has terompet like shapes and rectangular shape.Key words: Arabidopsis, KNAT1 gene, Phalaenopsis amabilis, shoo

PENGARUH FASILITAS KERJA, KEDISIPLINAN DAN PENGAWASAN TERHADAP KINERJA PEGAWAI DINAS PERIKANAN KOTA TANJUNGBALAI

The  formulation  of  the  problem  in  this  research  is:  How  do  work  facilities, discipline   and   supervision   influence   the   employees   performance   at   Dinas Perikanan  Kota Tanjungbalai.  This study aims to determine  the effect of work facilities,   discipline   and  supervision   on  employee   performance.   With   total sampling technique, the sample in this study was 41 people. The results showed; Work  facilities   partially   have  a  positive   effect  on  employee   performance; Discipline partially has a positive effect on employee performance; Partial supervision has a positive effect on employee performance; Work facilities, discipline and supervision have a positive and significant effect on employee performance

KAJIAN SIFAT FISIKA TANAH PADA PERTUMBUHAN TANAMAN MANGGIS (Garcinia mangostana L.) DI KABUPATEN LIMA PULUH KOTA

This research was aimed to evaluate physical properties of soils grown by mangosteen crops in Lima Puluh Kota Regency, especially in Kecamatan Payakumbuh and Kecamatan Guguk, in West Sumatra.   Soils were sampled by using sample rings for undisturbed and by using soil driller for disturbed samples on 0-20, 20-40, and 40-60 cm soil depth.  Soil samples were analyzed in Soil Laboratory Agriculture Faculty, Andalas University, Padang.  The results showed that soil texture in two locations was similar, that was silty loam for the 0-40 cm depth, and clay for the 40-60 depth.  However, generally soil organic matter content in Guguk was lower than that in Payakumbuh, that was medium to low, BV from low to high, total porosity from high to medium, and permeability from high to slightly slow.  Key  Words: Soil physical properties, mangostan