충청남도 남북교류 활성화 방안 연구

1. 연구 배경 및 목적 국민의 정부와 참여 정부의 대북 정책에 따라 남북한 교류가 활성화되고 있다. 실용정부에 들어서는 북한 핵문제로 인하여 남북 교류가 위축되고 있지만, 장기적으로 북한의 개방이 불가피하기 때문에 남북 교류도 다시 활성화될 것이다. 본 연구의 목적은 충청남도가 남북 교류 협력을 활성화하기 위한 제도와 기반의 구축, 교류 협력 사업 등을 발굴하는 데에 있다. 이를 위해서 충남과, 다른 지방자치단체의 남북 교류 추진실태 및 현황을 조사하고 그 성공 요인과 실패 요인을 분석하였다. 이를 바탕으로 충남이 추후 구축해야 할 법적, 물리적, 제도적, 기반을 제시하였다. - 이후 생략제 1 장 서론 제 2 장 남북교류에 관한 이론적 고찰 제 3 장 지방자치단체 남북협력 사업의 과정 제 4 장 지방자치단체의 남북교류 실태와 문제점 제5장 충청남도 남북교류협력 활성화 과제 제6장 결론 및 정책제언 참고문

충전 액적의 전기영동을 이용한 전기천공 랩온어칩 시스템 개발

DoctorMany microfluidic devices especially droplet-based microfluidic system, have adopted electric force as an actuation method, because the actuation method using electric force is easily integrated with various microfluidic systems. The direct charging and subsequent electrophoretic control of a droplet in a dielectric liquid have been studied by a number of researchers as a novel droplet manipulation method. The electrophoresis of a charged droplet (ECD), which is one of the droplet control methods with electric force, has some advantages as a droplet actuation method; a simple and straightforward principle, minimum contact with solid surface, easy controllable coalescence, and fast moving velocity. ECD method can be used for the miniaturized chemical and biological reactor and assay due to the precise control of an individual droplet In this thesis, we developed microfluidic devices based on the ECD and demonstrated various chemical and biological experiments to prove their potential as novel droplet-based microfluidic platforms. We have made a digital microfluidic system based on a direct electric charging and subsequent electrophoretic manipulation of droplets by simple fabrication at low cost. Digitally controlled two-dimensional droplet motions are demonstrated by digital polarity control of an array of electrode. By independent control of droplets and colorimetric detection, the coalescence and mixing of droplets can be analyzed quantitatively. The gelation of sodium alginate and the crystallization of calcium carbonate by multiple droplet translations and coalescence and the actuation of glassy carbon beads are demonstrated to show the versatile manipulation capability of the ECD chip system. The implications and potentials of the ECD based microfluidic platform are also discussed. A unique digital microfluidic electroporation (EP) system has been demonstrated successfully and shows higher transgene expression than that of conventional techniques, in addition to reliable productivity and feasible integrated processes. By systematic investigations into the effects of the droplet EP conditions for a wild-type microalgae, 1 order of magnitude higher transgene expression is accomplished without cell wall removal than the conventional bulk EP system. In addition, the newly proposed droplet EP method by a droplet contact charging phenomena shows a great potential for the integration of EP processes and on-chip cell culture providing easy controllability of each process. The implications of the accomplishments and future directions for development of the proposed technology are discussed. Furthermore, the continuous droplet electroporation system has been investigated to accomplish higher productivity of the electroporation by modifying the droplet electroporation system based on ECD method with nozzle electrodes, which are used for continuous inlet and outlet of the cell solution. Optimal conditions for continuous EP including applied electric potential and flow rate are investigated by numerical simulations. In numerical studies, the electric field distribution and electric field strength exerted on cells in the continuous EP system are compared with those of the static EP system. Continuous droplet EP experiments are performed and intracellular DNA delivery is confirmed by the confocal microscopy. The parallel continuous droplet EP system is also developed by introducing additional electrode pairs into the EP system for the better productivity

The development of a microfluidic device by the electrophoresis of a charged droplet

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Digital Microfluidic Electroporation System for Microalgae Transformation

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NON-CONVENTIONAL ELECTROPORATION METHOD BASED ON DROPLET CONTACT CHARGING PHENOMENON

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면역학적방법을 아용한 꽃게 헤모시아닌의 생화학적 특성에 관한 연구

학위논문(박사) - 한국과학기술원 : 생물공학과, 1988.8, [ viii, 103 p. ]Hemocyanin of Portunus trituberculatus was purified. SDS polyacrylamide gel electrophoresis (PAGE) were performed to investigate size homogeneity of hemocyanin. The electrophoretic pattern of purified hemocyanin with detergent, SDS in 7.5\% gel revealed a single band with a molecular weight of about 75,000 indicating a size homogeneity of the Portunus trituberculatus hemocyanin subunit. The purified hemocyanin was then subjected to alkaline electrophoresis without detergent. DAVIS-PAGE of hemocyanin in pH 7.5 showed polymorphism of monomers, hexamers, and higher polymers. In order to determine the charge heterogeneity of monomeric subunits, DAVIS-PAGE were performed. While SDS-PAGE showed no size heterogeneity of the monomeric subunit of Portunus trituberculatus, DAVIS-PAGE in high acrylamide concentration showed at least four monomeric subunits. In order to confirm the charge heterogeneity of Portunus trituberculatus hemocyanin, continuous polyacrylamide gel electrophoresis was performed. While nondissociating buffer (Tris/HCl-$CaCl_2$ buffer pH 7.5) demonstrated only polymeric forms without monomeric fractions, continuousPAGE in a dissociating buffer (Tris/HCl_EDTA buffer pH 8.9) showed that monomeric subunits of Portunus trituberculatus hemocyanin were resolved into four distinct bands, designated as I, II, III, and IV. This agreed with the results of DAVIS-PAGE in a nondissociating buffer to observe and isolate the polymeric forms of hemocyanin, and in a dissociating buffer to obtain the monomeric subunits of hemocyanin. To investigate the antigenic heterogeneity of hemocyanin subunits, the experiments of immunoelectrophoresis using anti-whole Portunus trituberculatus hemocyanin antiserum were carried out. The crossed immunoelectrophoresis (CIE) of whole hemocyanin revealed four distinct peaks, as four monomeric bands were observed in continuous-PAGE. These four peaks did not show any partial identity with each other, which suggested the occurrence of at le...한국과학기술원 : 생물공학과

A high throughput droplet based electroporation

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Digitally controlled droplet microfluidic system based on electrophoretic actuation.

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Numerical study on the injection method of reducing gases for minimizing greenhouse gas production in iron making process

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