社会主義システムの中での中国の金融改革 : 資金調達問題を中心に

埼玉学園大学2015年

台湾地区法院对通过两岸司法互助取得证言陈述之证据能力的认定及其发展趋势

调查取证是两岸司法互助的重要内容,而证人证言在通过司法互助途径取得的证据中占有重要的地位。如何认定通过两岸司法互助途径取得的证人证言的证据能力,直接决定着两岸司法互助的成效...2017年教育部人文社科重点研究基地重大项目“新形势下维护两岸关系和平发展的法治路径研究”(项目编号:17JJDGAT001)阶段性研究成

Advances in bacterial iron homeostasis and antibiotics killing mechanism

传统研究认为不同种类的抗生素有其各自不同的杀菌途径,然而近年人们发现了一条抗生素杀菌的共用通路,那就是当细菌细胞受到抗生素致死性刺激时,呼吸链代谢异常产生大量活性氧簇(ROS),而铁离子对ROS介导的杀菌至关重要。本文参阅近年来国内外研究结果,介绍了铁吸收、储存和利用有关的细菌铁代谢系统及其调控机制,重点讨论铁离子的调控及其与抗生素介导的ROS形成的相互作用关系。对于细菌耐药机制的研究与寻找新型药物作用靶点,探索有效控制手段和开发新药有重要意义。Previous research suggested that different antibiotics kill bacteria via various targets or mechanisms. However, recent study indicated that a common mechanism might be shared by most bactericidal antibiotics. When bacteria suffered lethal stress by antibiotics, reactive oxygen species(ROS) was induced by abnormal respiratory chain reaction in which iron played a key role. By reviewing current research progress regarding antibiotic resistance and iron related proteins in bacteria, including the iron absorbing, storage and consumption system in bacteria, this paper will mainly discuss the possible linkage/interaction between the regulation of ferrous ion and ROS induced by antibiotics. This common bacterial killing mechanism will have a significant impact on the future research for drugresistance, drug target screening, infection controlling and novel drug design.国家自然基金(No.81473251/81301474/31370166);; 福建省自然科学基金(No.2014J01139/2015J01345);; 国家重点基础研究发展计划(973计划)前期研究专项(No.2014CB560710

碳稳定同位素技术在植物水分胁迫研究中的应用

植物体的碳稳定同位素组成主要由植物本身的生物学特性决定, 但环境胁迫对其影响也十分明显。综述了碳稳定同位素 技术在研究植物水分利用效率、生物量高低及判断历史气候依据等研究领域的进展, 阐明了植物体的D13C 值对干旱、盐分及其 它环境因素的变化所引起的水分胁迫的响应, 并对碳稳定同位素对水分胁迫的响应机理进行了归纳和推断

Effects of La~(3+) on ATPase Activities of Plasma Membrane Vesicles Isolated from Casuarina Equisetifolia Seedlings under Acid Rain Stress

The effects of La3 + on the growth and the ATPases activities of plasma membrane (PM) vesicles isolated from Casuarina equisetifolia seedlings under artificial acid rain (pH 4. 5) stress were studied. The results show that the height , length of roots , fresh weight and PM H+2ATPase activites of Casuarina equisetifolia seedlings increase by the treatments of soaking seeds in LaCl3 solutions with lower concentrations , and those can reach their peak values by treating with 200 mg ·L - 1 La3 + . However , in comparison with the CK, those are inhibited by the higher La3 + concentrations ; PM Ca2 +2AT2 Pase activity is inhibited with the treatments of La3 + . The results also reveal that the H+2ATPase activity and the growth of cell enlarge have a remarkable positive correlation , and La3 + activating H+2ATPase can facilitate plant growth. La3 + al2 so can alleviate cytosolic acidification of plant under acid rain stress and indirectly maintain the stability of intracellular environment . In order to resistant to acid rain and accelerate the growth of Casuarina equisetifolia , the suitable range of La3 + concentrations to soak seeds for 8 h is 50～200 mg·L - 1

盐胁迫下红海榄脯氨酸与活性氧代谢特征研究

系统地研究了盐胁迫下红海榄脯氨酸和活性氧的代谢特征. 结果表明:1) 脯氨酸含量随着盐度的增加出现先 降后升的趋势,当盐度达到10 时,其含量达最低. 在无盐和高盐环境下脯氨酸的大量积累是植物细胞的适应性反应, 其含量的高低不宜作为红海榄的抗盐性指标. 2) 超量脯氨酸积累会影响CO2 的固定,降低叶片细胞内有机物的合成 量,导致高盐胁迫下叶片的肉质化程度降低. 3) 红海榄SOD 活性随盐度呈先降低后升高的趋势,其超氧负离子释放 速率与SOD 活性呈负相关. 在中高盐度下,其POD、CAT 活性迅速增加,可有效地清除由SOD 与O H2 产生的H2O2 ,避免了由于盐胁迫导致活性氧增加而对质膜造成的伤害

木麻黄质膜离子泵在酸雨条件下对镧的响应

研究镧对酸雨(pH 为4. 5) 胁迫下木麻黄幼苗早期生长和质膜离子泵ATP 酶活性的影响。结果表明: 较低浓度La3 + 浸种能使幼苗株高、 根长、鲜重以及质膜质子泵活性明显增强, 在200 mg·L - 1 La3 + 处理时达到最高增强值, 而高浓度的La3 + 浸种使之表现出抑制效应; 幼苗质 膜钙泵的活性受La3 + 抑制。质子泵活性与细胞的伸长生长呈显著正相关关系, 镧对质子泵的活化作用有助于促进植物的生长, 且适量的镧具有缓解酸雨胁迫下生长的植物细胞质酸化, 维持细胞内环境稳定的间接作用。为抗酸雨和促生长, 木麻黄经镧浸种8 h 的最适宜La3 + 浓度为50～200 mg·L - 1

CDK5-dependent BAG3 degradation modulates synaptic protein turnover

阿尔茨海默病（AD）是严重威胁人类健康的重大神经系统疾病，AD的发生发展与衰老密切相关，目前临床治疗方法十分有限。因此迫切需要从AD致病早期入手，发现和鉴定导致AD神经功能紊乱的机制和靶点，为AD的早期防治提供基础。张杰教授及其团队从高通量磷酸化蛋白质组学入手，系统研究了CDK5在神经细胞中的磷酸化底物，鉴定出了在蛋白质量控制中发挥重要功能的BAG3蛋白是CDK5的全新底物。课题组从磷酸化蛋白质组学入手，发现和阐明了细胞周期蛋白激酶5（CDK5）通过调控BAG3在维持突触蛋白水平调控中的作用机制，及其在阿尔茨海默病（AD）发生发展中的机理。 该研究是多个团队历时8年合作完成的，香港中文大学的周熙文教授、美国匹兹堡大学的Karl Herrup教授、美国Sanford-Burnham研究所的许华曦教授、美国梅奥医学中心的卜国军教授，厦门大学医学院的文磊教授、张云武教授、赵颖俊教授、薛茂强教授，军事医学科学院的袁增强教授等都参与了该工作。 厦门大学医学院2012级博士生周杰超等为文章的第一作者，张杰教授为通讯作者。Background Synaptic protein dyshomeostasis and functional loss is an early invariant feature of Alzheimer’s disease (AD), yet the unifying etiological pathway remains largely unknown. Knowing that cyclin-dependent kinase 5 (CDK5) plays critical roles in synaptic formation and degeneration, its phosphorylation targets were re-examined in search for candidates with direct global impacts on synaptic protein dynamics, and the associated regulatory network was also analyzed. Methods Quantitative phospho-proteomics and bioinformatics analyses were performed to identify top-ranked candidates. A series of biochemical assays were used to investigate the associated regulatory signaling networks. Histological, electrochemical and behavioral assays were performed in conditional knockout, shRNA-mediated knockdown and AD-related mice models to evaluate its relevance to synaptic homeostasis and functions. Results Among candidates with known implications in synaptic modulations, BCL2-associated athanogene-3 (BAG3) ranked the highest. CDK5-mediated phosphorylation on Ser297/Ser291 (Mouse/Human) destabilized BAG3. Loss of BAG3 unleashed the selective protein degradative function of the HSP70 machinery. In neurons, this resulted in enhanced degradation of a number of glutamatergic synaptic proteins. Conditional neuronal knockout of Bag3 in vivo led to impairment of learning and memory functions. In human AD and related-mouse models, aberrant CDK5-mediated loss of BAG3 yielded similar effects on synaptic homeostasis. Detrimental effects of BAG3 loss on learning and memory functions were confirmed in these mice, and such were reversed by ectopic BAG3 re-expression. Conclusions Our results highlight that neuronal CDK5-BAG3-HSP70 signaling axis plays a critical role in modulating synaptic homeostasis. Dysregulation of the signaling pathway directly contributes to synaptic dysfunction and AD pathogenesis.This work was supported by the National Science Foundation in China (Grant: 31571055, 81522016, 81271421 to J.Z.; 81801337 to L.L; 81774377 and 81373999 to L.W.); Fundamental Research Funds for the Central Universities of China-Xiamen University (Grant: 20720150062, 20720180049 and 20720160075 to J.Z.); Fundamental Research Funds for Fujian Province University Leading Talents (Grant JAT170003 to L.L); Hong Kong Research Grants Council (HKUST12/CRF/13G, GRF660813, GRF16101315, AoE/M-05/12 to K.H.; GRF16103317, GRF16100718 and GRF16100219 to H.-M,C.); Offices of Provost, VPRG and Dean of Science, HKUST (VPRGO12SC02 to K.H.); Chinese University of Hong Kong (CUHK) Improvement on Competitiveness in Hiring New Faculty Funding Scheme (Ref. 133), CUHK Faculty Startup Fund and Alzheimer’s Association Research Fellowship (AARF-17-531566) to H.-M, C. 该研究受到了国家自然科学基金、厦门大学校长基金、福建省卫生教育联合攻关基金等的资助

身延山久遠寺藏本朝文粹訓点データ集稿（一）

本データ集は、平成26年度前後期に開講した広島大学・大学院文学研究科の博士課程前期学生対象の授業において取り上げた身延山久遠寺蔵本朝文粋の訓読作業を基にしたものである

水文水资源管理对防洪减灾的意义

水文水资源不仅对水利发展及农业进步有着直接影响,对防洪减灾能力的提升也有着重要作用。文章从水文水资源管理的意义开始谈起,并结合水文水资源管理现状,提出合理的管理及防洪减灾措施,以促进水利工程的正常运转,推动水利行业的进一步发展。</jats:p