TERS Investigation of Surface Species on Silicon and the Electromagnetic Coupling Effect among Metal Nanostructures

界面现象广泛存在于自然界中，许多重要的物理化学过程发生在界面或表面上，从分子水平上深入表征各种界面（表面）的结构和过程对界面研究具有重要的意义。作为一种界面表征技术，针尖增强拉曼光谱（Tip-enhancedRamanspectroscopy，TERS）结合扫描微探针技术与拉曼技术，可提供高达单分子检测的灵敏度与15nm的空间分辨率。其原理是以激光激发金针尖或银针尖末端产生的高度局域化的电磁场，使尖端正下方附近样品的拉曼信号得以增强。本论文工作着重利用TERS这两个优点，开展了以下工作： 1 作为一种最重要的半导体材料，硅在微电子领域发挥着极其重要的作用。通过共价键将有机分子修饰到硅表面将赋...Interfaces widely exist in nature and wherein a lot of physical chemical processes occur. The in-depth characterization about the structures and the processes of the interfaces (surfaces) at the molecule level is of great significance to the interfacial researches. Tip-enhanced Raman spectroscopy, as a technique for characterizing the interface, combines the scanning probe microscopy and the Raman...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：2052006115201

Determination of Microamouts of Ammonia in Meat by Nessler's Reagent Spectrophotometry

本文提出了一种测定肉中粗氨的方法,可用于肉品新鲜度的检验。在常温下,纳氏试剂与肉中粗氨反应生成淡红棕色胶态化合物,最大吸收波长为400nm,表观摩尔吸光系数为1.404×104L/mol·cm,线性方程为y=0.52x+0.0001,r=0.9997,线性范围为0～1.0μg/ml,回收率在97.8%～104.8%之间。A method for the determination of the crude ammonia in meat was presented in this paper. The method based on the color reaction of ammonia-Nessler's Reagent in room temperature. The results showed that the apparent molar absorptivity is 1.404 ×104 L/mol·cm at the wavelength of 400nm. Beer's law is obeyed for the ammonia in the range of 0～1.0 μg/ml. The correlation coefficient is 0.9995. The recovery rate is 97.8%～104.8%. The method has been applied satisfactorily to crude ammonia in meat.福建省科技计划重点项目(2004Y012

Studies on activity of pork peroxidase using TMB

以3,5,3,′5′-四甲基联苯胺(TMB)为底物对猪肉过氧化物酶(POD)的酶学特性以及猪肉不同部位过氧化物酶活性水平进行了较为系统的研究,并以辣根过氧化物酶(HRP)为标准酶获得了酶活性标准曲线。猪肉过氧化物酶在温度38℃,pH 4.80时活性最高。酶浓度与酶反应初始阶段的酶活性有较好的线性关系,氢供体底物TMB的最佳浓度为2.80×10-4mol/L,浓度过大则会抑制酶的活性,氢受体底物过氧化氢的浓度与酶活性有较好的线性关系和较宽的线性范围(0~4.20×10-3mol/L)。猪肉不同部位如腰条(指外腰条)、里脊(指内腰条)、后腿、前腿、三层、腱子中POD活性大小不同,前三者的POD活性相近且活性较小,而后三者活性较为相近且活性较大。通过辣根过氧化物酶标准曲线可以求估待测样品中POD的活性值。The pork peroxidase(POD) and its activity in different parts of pork using 3,5,3′,5′-tetramethylbenzidine(TMB) were detected and studied.The POD activity was evaluated using horseradish peroxidase(HRP) as a standard enzyme.The pork POD kept its best activity at the temperature of 38 ℃ and pH of 4.80,respectively.The concentration of POD was linear with the POD activity at the initial stages of enzyme reaction.The optimal concentration of TMB for POD activity measurement was 2.80×10-4 mol/L,and the activity would be inhibited if the concentration of TMB was over the value.The concentration of H2O2 was also linear with the POD activity and the linear range was 0～4.20×10-3 mol/L.The POD activities of the pork in the part of outer-tenderloin,inner-tenderloin and hind leg nearly kept the same and lower than those of the part of foreleg,streaky pork and tendon.The activity value of sample could be estimated according to the standard curve of HRP.福建省科技计划重点(2004Y012)项目资