中药治疗糖尿病视网膜病变疗效Meta分析

目的:评价中药干预糖尿病视网膜病变(DR)的疗效。方法:运用循证医学Cochrane系统评价方法,检索Pub Med、中国知网(CNKI)2010-2016年间发表的有关中药治疗DR的文献,由2位评价者独立筛选文献、数据提取和评价纳入研究的偏倚风险后,采用Rev Man5.3软件进行Meta分析。结果:共纳入17篇文献,共1756余例患者。Meta分析结果显示,中药组对临床疗效、视力、中医证候、眼底、视网膜循环时间效应、视网膜毛细血管渗漏范围、视网膜毛细血管无灌注区的效应均较对照组效果为佳。结论:中药干预糖尿病视网膜病变在改善患者视力、中医证候、眼底病变,降低视网膜循环时间,缩小视网膜毛细血管渗漏范围、无灌注区等方面具有一定的优势。国家自然科学基金资助项目(编号81673661

疏肝法对肝郁型多囊卵巢综合征疗效系统性分析

目的:运用Meta分析总结中医疏肝法对肝郁型多囊卵巢综合征(Polycysticovarysyndrome,PCOS)治疗的临床效果。方法:检索中文中国知网、维普、万方以及英文Pubmed、CochraneLibrary等数据库自建刊以来至今相关的文献,用Revman5.3软件进行分析。结果:共检索文献2030篇,最终纳入RCT文献14篇,共计1057例患者,其中治疗组529例、对照组528例。结果:与对照组西药相比,疏肝法治疗组在临床总有效率、排卵率、周期排卵率及妊娠率等方面差异具有统计学意义,且无明显偏倚。结论:运用疏肝法对肝郁型多囊卵巢综合征治疗后临床总有效率以及排卵率、周期排卵率、妊娠率均有显著提高,且效果优于西药对照组,说明运用中医辨证治疗PCOS患者尤其要重视肝郁症,对临床治疗多囊卵巢综合征具有指导意义。国家自然科学基金面上项目(No:81673661

参苓白术散对高脂血症小鼠甘油三脂水平的影响研究

目的:探讨参苓白术散对高脂血症小鼠甘油三酯以及基础指标(体重、血糖)等的影响。方法:将8～10周龄C57BL/6小鼠随机分为对照组(普通饲料+生理盐水灌胃)、模型组(高脂饲料+生理盐水灌胃)、参苓白术散组(高脂饲料+参苓白术散灌胃,灌胃剂量10g/kg)。高脂饲养小鼠12周,每周监测体重、血糖指标,于实验结束时收取血浆,测定血液中葡萄糖、甘油三脂、胰岛素水平。在收取组织前做胰岛素耐量(ITT)、葡萄糖耐量(GTT)实验以评估胰岛功能及胰岛素敏感性。结果:与对照组小鼠比较,模型组小鼠甘油三脂水平均显著增高(P<0.05),参苓白术散组能明显降低高脂造成的高甘油三脂血症,且差异有统计学意义(P<0.05)。模型组小鼠血糖及体重较对照组明显升高(P<0.05),葡萄糖耐量呈现受损趋势,这一改变并未因参苓白术散应用而改变。参苓白术散组小鼠血液中胰岛素水平高于模型组小鼠。结论:参苓白术散可改善高脂喂养小鼠的高甘油三脂血症,血糖及体重无改善,可能与药物剂量存在一定关系

Optimization of β-Cyclodextrin Inclusion Technology of Volatile Oil from Pueraria Compound by Orthogonal Design

目的优选葛根复方挥发油的包合工艺,提高制剂的稳定性。方法采用饱和水溶液法制备包合物,以挥发油中藁本内酯、桂皮醛、甲基丁香酚包合率及包合物得率为综合评价指标,采用正交试验法考察挥发油与β-环糊精的比例、包合时间、包合温度对包合工艺的影响,优化得到葛根复方中挥发油的最优包合工艺。结果葛根复方中挥发油的最佳包合工艺条件为挥发油与β-环糊精比例为1∶8,包合温度为30℃,包合时间为1h,验证试验中,藁本内酯、桂皮醛及甲基丁香酚的包合率为别为86.58%±0.009%,73.34%±0.001%,68.89%±0.020%,包合物得率为67.98%±0.047%。结论所确定的包合工艺挥发油的包合率高、工艺稳定可行。Objective To obtain the optimized technology of volatile oil from Pueraria Compound with β-Cyclodextrin（ β-CD）. Methods The inclusion compound was prepared by saturated peroxide solution method. In order to optimize inclusion technology,orthogonal design was performed,with the content of multi-target ingredient,such as ligustilide,cinnamic aldehyde,methyleugenol and the yield of inclusion as comprehensive evaluation indexes. Three factor was performed,the ratio of volatile oil（ m L） to β-CD（ g）,the inclusion temperature and the inclusion time. Results The optimum conditions for inclusion process were as follow： the ratio of volatile oil（ m L） to β-CD（ g） was 1： 8,the inclusion temperature was 30℃ and the inclusion time was 1h. With the optimum inclusion condition,the content of ligustilide,cinnamic aldehyde and methyleugenol and the yield of inclusion were 86. 58% ± 0. 009%,73. 34% ± 0. 001%,68. 89% ± 0. 020%,and 67. 98% ± 0. 047%,respectively.Conclusion The optimum inclusion condition was stable and reproducible with a high inclusion yield.国家自然科学基金（No.81270901）;上海市教委基金（No.15cxy21

Optimization of extraction for volatile oils and water-soluble constituents in Compound Gegen Decoction

目的优化葛根复方挥发油和水溶性成分提取工艺。方法以浸泡时间、提取时间、加水量为影响因素,综合评分(藁本内酯、桂皮醛、甲基丁香酚含有量,葛根素、阿; 魏酸转移率,干膏得率)为评价指标,正交试验优化提取工艺。结果最佳条件为浸泡时间30 min,提取2次,每次加入10倍量水,第1次提取5; h以收集挥发油,第2次提取1 h以收集水溶性成分,藁本内酯、桂皮醛、甲基丁香酚含有量分别为0.806、1.111、1.635 mg; /g,葛根素、阿魏酸转移率分别为61.62%、73.38%,干膏得率为34.42%。结论该方法稳定可行,可用于提取葛根复方挥发油和水溶性成分。国家自然科学基金; 上海市教委基

健脾化湿法治疗非酒精性脂肪肝的Meta分析

目的：系统评价健脾化湿法对非酒精性脂肪肝（NAFLD）患者肝功能、血脂、B超等指标改善的有效性。方法：在PubMed和知网、万方以及维普等数据库中检索健脾化湿法治疗NAFLD的临床随机对照试验的相关文献,研究人员采用Rev Man 5.3对其进行数据提取和整理。结果：14项研究,1110例非酒精性脂肪性肝病例被纳入研究,其中健脾化痰湿法605例和西药组505例。数据显示健脾化湿法组临床有效率（OR=1.20,95%CI：1.11～1.31,P〈0.001）、肝功能[ALT（MD=-4.51,95%CI：-6.79～-2.23,P〈0.001）、AST（MD=-2.57,95%CI：-4.07～-1.08,P〈0.001）、GGT（MD=-10.82,95%CI：-19.92～-1.73,P〈0.05）]、血脂[TC（MD=-0.59,95%CI：-0.84～-0.34,P〈0.001）、TG（MD=-0.35,95%CI：-0.52～-0.17,P〈0.001）、HDL-C（MD=0.2,95%CI：0.06～0.33,P〈0.05）、LDL-C（MD=-0.51,95%CI：-0.9～-0.13,P〈0.05）]、B超改变（OR=2.74,95%CI：1.36～5.55,P=0.005）均优于西药组（均P〈0.05）。结论：与单纯西药相比,健脾化湿法治疗NAFLD有效且安全,但由于提取的文献存在病例数少、随机方法不明确、未分配隐藏、未发现双盲试验等诸多问题,需要开展更为严格的随机对照试验提供临床证据。国家自然科学基金（81673661

Optimization of beta - Cyclodextrin Inclusion Technology of Volatile Oil from Pueraria Compound by Orthogonal Design

目的优选葛根复方挥发油的包合工艺,提高制剂的稳定性。方法采用饱和水溶液法制备包合物,以挥发油中藁本内酯、桂皮醛、甲基丁香酚包合率及包合物得率为综; 合评价指标,采用正交试验法考察挥发油与beta; -环糊精的比例、包合时间、包合温度对包合工艺的影响,优化得到葛根复方中挥发油的最优包合工艺。结果葛根复方中挥发油的最佳包合工艺条件为挥发油与be; ta -环糊精比例为1:8,包合温度为30℃,包合时间为1h,验证试验中,藁本内酯、桂皮醛及甲基丁香酚的包合率为别为86.58% ; 0.009%,73.34% 0.001%,68.89% 0.020%,包合物得率为67.98% ; 0.047%。结论所确定的包合工艺挥发油的包合率高、工艺稳定可行。Objective To obtain the optimized technology of volatile oil from; Pueraria Compound with beta - Cyclodextrin (beta - CD). Methods The; inclusion compound was prepared by saturated peroxide solution method.; In order to optimize inclusion technology,orthogonal design was; performed,with the content of multi - target ingredient,such as; ligustilide,cinnamic aldehyde, methyleugenol and the yield of inclusion; as comprehensive evaluation indexes. Three factor was performed,the; ratio of volatile oil (mL) to beta - CD (g),the inclusion temperature; and the inclusion time. Results The optimum conditions for inclusion; process were as follow:the ratio of volatile oil (mL) to beta - CD (g); was 1:8,the inclusion temperature was 30℃ and the inclusion time was 1h.; With the optimum inclusion condition,the content of ligustilide,cinnamic; aldehyde and methyleugenol and the yield of inclusion were 86.58% ; 0.009%,73.34% 0.001%,68.89% 0.020%,and 67.98% 0.047%,respectively.; Conclusion The optimum inclusion condition was stable and reproducible; with a high inclusion yield.国家自然科学基金; 上海市教委基

Puerarin Alleviates Neuropathic Pain by Inhibiting Neuroinflammation in Spinal Cord

National Natural Science Foundation [30973912]; Suhuan Liu [81270901]; Xuejun Li [81073113]; Key Project of Fujian Provincial Science and Technology Planning programs [2012D60]; Xiamen Innovation Programfor Outstanding Youth Scientist [2011S0446]; Xiamen Science and Technology Bureau (Xiamen Research Platform for Systems Biology of Metabolic Disease) [3502Z20100001]Neuropathic pain responds poorly to drug treatments, and partial relief is achieved in only about half of the patients. Puerarin, the main constituent of Puerariae Lobatae Radix, has been used extensively in China to treat hypertension and tumor. The current study examined the effects of puerarin on neuropathic pain using two most commonly used animal models: chronic constriction injury (CCI) and diabetic neuropathy. We found that consecutive intrathecal administration of puerarin (4-100 nM) for 7 days inhibited the mechanical and thermal nociceptive response induced by CCI and diabetes without interfering with the normal pain response. Meanwhile, in both models puerarin inhibited the activation of microglia and astroglia in the spinal dorsal horn. Puerarin also reduced the upregulated levels of nuclear factor-kappa B (NF-kappa B) and other proinflammatory cytokines, such as IL-6, IL-1 beta, and TNF-alpha, in the spinal cord. In summary, puerarin alleviated CCI- and diabetes-induced neuropathic pain, and its effectiveness might be due to the inhibition of neuroinflammation in the spinal cord. The anti-inflammation effect of puerarin might be related to the suppression of spinal NF-kappa B activation and/or cytokines upregulation. We conclude that puerarin has a significant effect on alleviating neuropathic pain and thus may serve as a therapeutic approach for neuropathic pain

Fructus Xanthii Attenuates Hepatic Steatosis in Rats Fed on High-Fat Diet

Xiamen Science and Technology Bureau (Xiamen Research Platform for Systems Biology of Metabolic Disease) [3502Z20100001]; National Natural Science Foundation [30973912, 81073113, 81270901]Fructus Xanthii (FX) has been widely used as a traditional herbal medicine for rhinitis, headache, cold, etc. Modern pharmacological studies revealed that FX possesses anti-inflammatory, anti-oxidative, and anti-hyperglycemic properties. The present study was designed to investigate the effects of FX on glucose and insulin tolerance, and hepatic lipid metabolism in rats fed on high-fat diet (HFD). Hepatic steatosis was induced by HFD feeding. Aqueous extraction fractions of FX or vehicle were orally administered by gavage for 6 weeks. Body weight and blood glucose were monitored. Glucose and insulin tolerance test were performed. Liver morphology was visualized by hematoxylin and eosin, and oil red O staining. Expression of liver lipogenic and lipolytic genes was measured by real-time PCR. We showed here that FX improved glucose tolerance and insulin sensitivity in HFD rats. FX significantly decreased the expression of lipogenic genes and increased the expression of lipolytic genes, ameliorated lipid accumulation and decreased the total liver triglyceride (TG) content, and thus attenuated HFD-induced hepatic steatosis. In conclusion, FX improves glucose tolerance and insulin sensitivity, decreases lipogenesis and increases lipid oxidation in the liver of HFD rats, implying a potential application in the treatment of non-alcoholic fatty liver disease

An Aqueous Extract of Radix Astragali, Angelica sinensis, and Panax notoginseng Is Effective in Preventing Diabetic Retinopathy

Xiamen Science and Technology Bureau [3502Z20100001]; National Natural Science Foundation [30973912, 81270901, 81073113]Diabetic retinopathy (DR), in which inflammation has been implicated playing important roles, is one of the most common diabetes complications. Dang Gui Bu Xue Tang (DBT), an aqueous extract of Radix Astragali and Radix Angelica sinensis, is a classical prescription in Traditional Chinese Medicine for treating inflammation and ischemic diseases. Here, we investigated the effects of a modified recipe of DBT, with addition of Panax notoginseng, in treating diabetic retinopathy. An aqueous extract of Radix Astragali, Radix Angelica sinensis, and Panax notoginseng (RRP) was given to Goto-Kakizaki (GK) rats and streptozotocin-induced Sprague-Dawley (SD) rats. Leukostasis, vascular leakage, and acellular capillaries in retinal vasculature of animals were determined. Expression of retinal inflammatory biomarkers was assessed. We found that RRP reduced leukostasis, acellular capillaries, and vascular leakage compared to diabetic control rats. We also found that RRP decreased the expression of inflammatory factors including IL-1 beta, IL-6, TNF-alpha, NF-kappa B, MCP-1, ICAM-1, or VCAM-1 in the retinas of GK rats and reversed high glucose-induced inhibition of endothelial cell migration and proliferation in vitro. We conclude that RRP has a potent effect in preventing the pathogenesis and/or progression of DR and thus may serve as a promising nontoxic therapeutic approach of DR