蒿芩清胆汤及其拆方对湿热型流感病毒性肺炎及NF-κB水平的作用研究

目的:通过观察蒿芩清胆汤及其拆方对湿热型流感病毒性肺炎及NF-κB的干预作用,以期探讨其治疗机制。方法:通过气候仓、肥甘饮食加流感病毒滴鼻感染造模成功后,蒿芩清胆汤及其拆方组以相应中药干预,比较各组肺指数、光镜下病变程度半定量、肺组织NF-κB mRNA表达水平。结果:蒿芩清胆汤及其拆方均能不同程度降低肺指数、肺部病理改变及NF-κB mRNA表达水平,其中全方效果最佳。结论:蒿芩清胆汤具有抗湿热型流感病毒性肺炎的作用,其机制可能与抑制NF-κB活化从而减少其下游炎性细胞因子分泌,截断"炎症瀑布"效应有关

低龄DDH患儿初次开放复位术中股骨截骨必要性的前瞻性随机对照试验研究方案

回顾性研究发现,股骨截骨术并非低龄DDH患儿手术治疗所必须的,而应根据患儿具体情况合理选择;本研究通过多中心前瞻性研究进一步探讨对于18个月至3岁DDH患儿是否要行股骨截骨术。FSODDH项目为一项多中心前瞻性随机对照临床试验研究。研究设计拟招募200名单侧低龄DDH患儿,随机分成股骨截骨组(n=100)和股骨不截骨组(n=100)。股骨截骨组患儿全部采取髋关节开放复位术、骨盆截骨术及股骨截骨术治疗;股骨不截骨组患儿全部采取髋关节开放复位术及骨盆截骨术治疗;术后定期随访至少2年。收集术前、术后1周、术后1个月、术后3个月、术后6个月、术后1年及术后2年的髋关节正位X线影像资料,比较两组患儿的髋臼指数、股骨头坏死率、再脱位率、术中出血量、手术时间和住院天数等临床指标。这一多中心前瞻性研究将能为低龄DDH患儿是否需行股骨截骨术提供循证医学证据

Calibration and Preliminary Application of Linear Quantitation Standard for Anti-HEV IgG Antibody

目的建立抗戊型肝炎病毒(Anti-HEV)IgG抗体的定量线性标准品,并进行初步应用。方法利用抗-HEV IgG和抗-HEV IgM ELISA检测试剂筛选出1份抗-HEV IgG阳性血清L9,经基因1型和4型的HEV ORF2C-端抗原及239抗原进行Western blot确认后,用WHO定量标准品,由3个实验室协作标定,利用量反应平行线法计算其抗-HEV IgG的含量。考察已标定的L9血清的稳定性,并用所标定的1.5倍系列稀释的血清对国内外6家抗-HEV IgG试剂的灵敏度进行检测。选择一灵敏度较高的试剂,在其线性范围内取L9的5个稀释度作为抗-HEV IgG抗体定量线性标准,对高、中、低浓度的3份临床血清重复检测5次,考察其重复性;对实验感染猴的系列血清中抗-HEV IgG含量进行定量检测,考核该定量线性标准品的应用效果;并对每次定量试验中的线性方程进行分析,确定相关系数r值和斜率k值的范围。结果经国内外试剂检测筛选出的阳性血清L9与基因1型和4型的HEV ORF2 C-端抗原及239抗原均有阳性反应。经协作标定,L9血清抗-HEV IgG含量为16.9U/ml。L9血清在-20℃下保存6、12、18个月,2～8℃保存24、48、96h后,定量结果均在95%置信区间内,且抗-HEV IgG含量均未明显下降。6家抗-HEVIgG检测试剂灵敏度差异较大,范围为0.03～5.00U/ml。确定L9血清从0.42U/ml开始的5个1.5倍系列稀释度,作为某一试剂抗-HEVIgG抗体定量线性标准品。利用该线性定量标准检测高、中、低浓度的3份临床血清,定量结果重复性较好;对实验感染猴系列血清进行定量检测,结果可有效地反映抗体水平变化趋势;94%的定量检测试验,r≥0.98,1.15≥k≥0.95。结论已建立了抗-HEVIgG抗体定量线性标准品,可用于疫苗免疫原性评价和流行病学调查。Objective To develop a linear quantitation standard for anti-hepatitis E virus (HEV) IgG antibody. Methods An anti-HEV IgG positive serum sample L9 was screened by using Anti-HEV IgM ELISA kit and Anti-HEV IgG ELISA kit and confirmed by Western blotting with HEV ORF2 C-terminal antigen of genotypes 1 and 4 and 239 antigen, then calibrated by 3 laboratories using WHO quantitation standard. Calculate the anti-HEV IgG content by dose-response parallel line assay, and evaluate the stability of calibrated serum sample. The sensitivities of 6 domestic and imported anti-HEV IgG ELISA kits were evaluated with the calibrated serum sample diluted 1. 5-fold serially. A linear quantitation standard for anti-HEV IgG antibody consisted of 5 dilutions of L9 within the linear determination range of a highly sensitive kit and evaluated for reproducibility by repeat test for 3 clinical serum samples, at high, moderate and low anti-HEV IgG contents respectively, for 5 times. The anti-HEV IgG contents in serum samples of HEV-infected monkeys were determined by the standard, and the determination curves were analyzed to define correlation coefficient r and slope k. Results Serum sample L9 showed positive reaction with HEV ORF2 C-terminal antigen of genotypes 1 and 4 and 239 antigen, and its anti-HEV IgG content was calibrated as 16. 9 U / ml. After storage at -20℃ for 6, 12 and 18 months or at 2 ～ 8℃ for 24, 48 and 96 h, all the quantitative determination results were within the 95% CI, and anti-HEV IgG content showed no significant decrease. The sensitivities of 6 kits evaluated with the L9 ranged from 0. 03 to 5. 00 U / ml. The linear quantitation standard for anti-HEV IgG antibody consisted of 5 dilutions of L9, starting from a concentration of 0. 42 U / ml. The determination results of 3 clinical serum samples showed good reproducibility of the standard. The determination results of sera of HEV-infected monkeys reflected the change of antibody level effectively. The r values of 94% of quantitative determination curves were not less than 0. 98, and the k values ranged from 1. 15 to 0. 95. Conclusion A linear quantitation standard for anti-HEV IgG antibody was established, which was suitable for the evaluation of immunogenicity and epidemical investigation of vaccine

HYDROLOGICAL OBSERVATION IN AN EVERGREEN BROAD LEAVED FOREST IN THE WUYI MOUNTAINS

[中文文摘]对武夷山甜槠林成熟林水文学效应的研究结果表明：观测期间，年大气降水量2678.78ｍｍ，林内雨量2182.04ｍｍ，林冠截留量为496.74ｍｍ；林内雨中，穿透雨量2082.08ｍｍ，树干茎流99.96ｍｍ；到达甜槠林地作用面的林内雨量中，被地表枯枝落叶层截留的雨量为159.84ｍｍ，地表径流量11.6ｍｍ，地下渗流量109.32ｍｍ，其余林内雨量形成土壤含水量增量并由地表物理蒸发、根系吸收以及植物蒸腾所消耗；甜槠林地o~20０ｃｍ土层水分初渗率78.6ｍｍ·ｍｉｎ－１，稳渗率１５．５ｍｍ·ｍｉｎ－１，达到稳渗历时４５ｍｉｎ，地表枯枝落叶最大持水量5.2ｍｍ，土壤蓄水量154.0ｍｍ。与国内其它地区不同类型的森林相比较，武夷山甜槠林具有较强的水文学效能。[英文文摘]Hydrological observations were made in a 76 year old Castanopsis eyrei forest of the Wuyi Mountains.The results are as follows:(1)In the study period during 1993～1994,annual gross precipitation,net precipitation (throughfall was 208204mm and stemflow was 9996mm)and interception loss were 267878mm,218204mm and 49674mm respectively.Interception rate was 1845%.(2)Net precipitation,the part of gross precipitation appearing on the forest floor,was redistributed through different routes.The part absorbed by litter layer was an annual amount of 15984mm,and accounting for 733%.The parts as surface runoff and below ground drainage were annual amounts of 116mm and 10932mm respectively,and accounting for0.53% and 5.01%,respectively.The rest was kept in soil and then lost to the atmosphere by means of ground physical evaporation or plant transpiration as absorbed by the roots.Its amount was 190128mm,and accounting for 87.13%.(3) The initial infiltration rate and stable infiltration rate for the soil layer of 0～20cm were 786mm·min-1 and 155mm·min-1 respectively.The time needed to arrive at the stable state was 45min.Water storage capacity of soil was 15041t·hm-2.The maximum water holding capacity of litter layer was 52mm.福建省自然科学基金,武夷山自然保护区管理局资

螺旋型多间隙高频谐振装置

<span style="color: rgb(69, 69, 69); font-family: Arial, Helvetica, sans-serif; line-height: 21px; text-indent: 24px;">&nbsp; &nbsp; &nbsp; &nbsp; 本实用新型涉及高频谐振装置，尤其涉及重离子加速器使用的高频谐振装置。一种螺旋型多间隙高频谐振装置，包括腔体，在腔体上设有耦合器支架，所述空腔内设有同轴设置的第一漂移管、第二漂移管、第三漂移管、第四漂移管、第五漂移管和第一螺旋臂和第二螺旋臂所述的耦合器支架将耦合器支设在所述的第一螺旋臂和第二螺旋臂之间。本结构采用低电压设计，提高电压冗余度，对将来进一步提高电压具有很大的潜力；在结构和尺寸上极大的节省了建造成本；随着二级量的改善，可以提高整体聚束和加速的稳定性。</span

Multi-channel Localization Technology Based on Sensor Networks

针对无线传感器网络中的信号强度随信道和位置的变化特征进行研究,通过理论分析和实验验证的方式刻画了信号强度与信道、位置之间的关系.在此基础上,提出了使用多信道信号强度分布中心值进行定位的方法.实验结果表明,本文使用的方法可以提高测距和定位的精度,同时避免了复杂的运算过程,适合在现有的无线传感器网络上应用.</p

Review on the Study of Forest Precipitation Chemistry

森林降水化学是研究大气降水的化学物质输入及其对森林生态系统生物物质循环影响规律的一门分支学科。该文综述了有关森林降水化学研究的几个方面。其中包括森林降水化学的范畴，有关的水文学与生态学概念及其发展；世界范围内森林降水化学物质输入规律；森林降水化学的动态变化规律及其影响因子。最后提出了降水化学研究中应注意的几个问题。Forest precipitation chemistry is a newly-developed branch subject of ecology and Foresthydrology concerned with the research of input of chemical substances by atmospheric precipitationand its eFFects on the biological cycling of minerals in Forest ecosystem.In this paper,some aspectsabout Forest precipitation chemistry study are reviewed,including research scope,related Concepts andevolution;changes of inputs of chemical substances by precipitation in diFFerent Forests in the world;spatial and temporal dynamics of precipitation chemistry and Factors aFFecting it.Some questions areraised For attention and Further research in the Future study

一种基于跳频技术的RSSI定位方法

An RSSI positioning method based on frequency-hopping spread spectrum technology, comprising: calibration stage: measuring the RSSI values of a plurality of channels at fixed points, and recording and calculating the ranging parameters in an RSSI ranging model; system preparation: deploying a positioning anchor node, and realizing synchronization between a target node and the anchor node; conducting communication on the target node by respectively utilizing a plurality of channels to obtain the RSSI values; signal processing stage: processing the RSSI into signal strength amplitude and performing optimization; and positioning stage: calculating a distance and the target node position on a positioning server according to each of the signal strength. The present invention solves the problem that low RSSI positioning precision cannot satisfy the actual requirements because a traditional RSSI positioning method is limited to factors such as multipath signal transmission, co-channel interference, obstacle interference and low coordinate calculation precision of a trilateration method