Spatial transcriptomics reveals discrete tumour microenvironments and autocrine loops within ovarian cancer subclones

High-grade serous ovarian carcinoma (HGSOC) is genetically unstable and characterised by the presence of subclones with distinct genotypes. Intratumoural heterogeneity is linked to recurrence, chemotherapy resistance, and poor prognosis. Here, we use spatial transcriptomics to identify HGSOC subclones and study their association with infiltrating cell populations. Visium spatial transcriptomics reveals multiple tumour subclones with different copy number alterations present within individual tumour sections. These subclones differentially express various ligands and receptors and are predicted to differentially associate with different stromal and immune cell populations. In one sample, CosMx single molecule imaging reveals subclones differentially associating with immune cell populations, fibroblasts, and endothelial cells. Cell-to-cell communication analysis identifies subclone-specific signalling to stromal and immune cells and multiple subclone-specific autocrine loops. Our study highlights the high degree of subclonal heterogeneity in HGSOC and suggests that subclone-specific ligand and receptor expression patterns likely modulate how HGSOC cells interact with their local microenvironment

Rabhadh Gan Ghníomh / Alarmed and Inactive

ALARMED AND INACTIVE is a multi-media exploration of the theme of climate emergency, through the responses of individual artists. This bi-lingual performance will fuse experimental and improvised music – played by Matthew Jacobson (percussion); Una Monaghan (harp, electronics); and Cuan Ó Seireadáin (French horn) – with visuals by Vivienne Griffin, sculpture by Nóirín Nic Alastair, and poetry and prose by Nandi Jola, Nithy Kasa, Ailbhe Ní Ghearbhuigh, Daithí Ó Muirí, and Róisín Sheehy.Tá alltacht an domhain orainn go léir, mar chine, ar fheiceáil dúinn straicí móra d’Amazonia trí thine. Taifeadadh teocht scanrúil i ngach tír nach mór. Tá na farraigí ag ardú, gaineamhlaigh ag leathnú amach, barra ag teip agus laghdú catastrófach ar bhithéagsúlacht na cruinne. D’iarr IMRAM ar Úna Monaghan, cláirseoir, cumadóir agus ealaíontóir fuaime, leiriú ilmheánach a chruthú agus a bhainistiú chun an téama seo a fhiosrú, géarchéim na haeráide. Sa saothar is deanaí uaithi, tá meascán den cheol traidisiúnta, dealbhoireacht chré-umha, ealaín fuaime agus braiteoirí gluaiseachta. Beidh ceol turgnamhach agus tobchumtha le cloisteáil sa seó