Evaluation of the ingestive behaviour of the dairy cow under two systems of rotation with slope

The ingestive behaviour of grazing animals is modulated by the vegetation characteristics, topography and the type of stocking method. This research was carried out in 2019, at the Rumipamba CADER-UCE. It aimed to evaluate the impact of two contrasting stocking methods of dairy cows grazing a pasture with an average of slope >8.5%. Four dairy cows were set to graze a 0.4 ha paddock for 5 days for continuous stocking methods, while for the electric fence methods the dairy cows were restricted to 0.2 ha and the fence was moved uphill every 3 hours, repeating this process four times a day. Cow were equipped with activity sensors for 12 h per day. The whole procedure was repeated 2 times after realizing an equalization cuts and both paddocks, a rest time of 30 days and a random reassignment of paddocks to one of the treatments. The cows showed a difference in terms of the percentage of grazing P=0.0072, being higher with the electric fence (55% of the measurement time). From rising-plate-meter estimates of available biomass along the grazing periods, we calculated despite similar forage allowances (electric fence = 48.06 kg DM/cow/d and continuous = 48.21 DM/cow/d) a higher forage intake was obtained in the electric fence treatment (17.5 kg DM/cow/d) compared the continuous stocking (15.7 kg DM/cow/d) (P=0.006). In terms of milk production animals grazing under the differences electrical fence stocking method tended (P=0.0985) to produce more milk (17.39 kg/d) than those grazing in the continuous system (15.16 kg/d) due to the influence of the slope (P=0.05), while for milk quality the protein content was higher for the electric fence (33.7 g/l) than the continuous method (30.5 g/l) (P=0.039). None of the other milk properties differed between methods (P>0.05)

Modeling Eye Tracking Data with Application to Object Detection

This research focuses on enhancing computer vision algorithms using eye tracking and visual saliency. Recent advances in eye tracking device technology have enabled large scale collection of eye tracking data, without affecting viewer experience. As eye tracking data is biased towards high level image and video semantics, it provides a valuable prior for object detection in images and object extraction in videos. We specifically explore the following problems in the thesis: 1) eye tracking and saliency enhanced object detection, 2) eye tracking assisted object extraction in videos, and 3) role of object co-occurrence and camera focus in visual attention modeling.Since human attention is biased towards faces and text, in the first work we propose an approach to isolate face and text regions in images by analyzing eye tracking data from multiple subjects. Eye tracking data is clustered and region labels are predicted using a Markov random field model. In the second work, we study object extraction in videos using eye tracking prior. We propose an algorithm to extract dominant visual tracks in eye tracking data from multiple subjects by solving a linear assignment problem. Visual tracks localize object search and we propose a novel mixed graph association framework, inferred by binary integer linear programming. In the final work, we address the problem of predicting where people look in images. We specifically explore the importance of scene context in the form of object co-occurrence and camera focus. The proposed model extracts low-, mid- and high-level and scene context features and uses a regression framework to predict visual attention map. In all the above cases, extensive experimental results show that the proposed methods outperform current state-of-the-art

Actas de las XXXIV Jornadas de Automática

Postprint (published version

Fluorescent gene reporters in human pluripotent stem cells : as model for studying human heart development and cardiomyocyte differentiation

It is critical to gain knowledge in the underlying mechanisms that control human cardiovascular developm ent, which helps us to understand the onset of congenital cardiovascular diseases, and to develop optimal culture methods for efficient in vitro cardiomyocyte differentiation from hPSCs, which are of interest for final translational applications including screening and efficacy assays for disease modelling, drug discovery and development, personalized medicine, and perhaps the regeneration of cardiovascular tissues for therapeutic purposes. In this thesis, we show how genetic manipulation of human pluripotent stem cells (hPSCs), resulting in the genomic integration of a fluorescent protein encoding sequence at the locus of a key cardiac transcription factor, allows us to visualize and isolate early pre-cardiac progenitors subpopulations, and to study the molecular mechanisms involved in their further differentiation to cells of the cardiac lineage, including smooth muscle cells, endothelial cells, and cardiomyocyte subtypes.Rembrandt Institute for Cardiovascular Research Nederlandse Hartstichting Stichting ProefdiervrijLUMC / Geneeskund

Treatment of Later Humoral Rejection with Anti-CD20 Monoclonal Antibody Rituximab: A Single Centre Experience

Humoral or vascular rejection is a B cell-mediated production of immunoglobulin (Ig) G antibody against a transplanted organ that results in immune complex deposition on the vascular endothelium, activation of the complement cascade, production of endothelial dysfunction and regional ischaemic injury