Field-Effect Sensors

This Special Issue focuses on fundamental and applied research on different types of field-effect chemical sensors and biosensors. The topics include device concepts for field-effect sensors, their modeling, and theory as well as fabrication strategies. Field-effect sensors for biomedical analysis, food control, environmental monitoring, and the recording of neuronal and cell-based signals are discussed, among other factors

Carbon nanotube field-effect sensors for single-molecule detection

This thesis describes a detection system for single molecules based on individual single-walled carbon nanotube field-effect sensors. The sensitivity, spatial confinement and transducer gain of the sensor is derived from a conductance controlled electrochemically created defect, which is also chemically reactive. An automated microfluidic system is designed to enable long and stable measurements of the carbon nanotube device in aqueous environment with temperature control of ±0.1°C. A probe DNA can be covalently attached to the defect through an amide bond and the conductance is modulated when a target DNA binds to the probe. As a result, the conductance shows a traditional random telegraph signal and fluctuates between a hybridized and melted state. By monitoring the conductance as a function of temperature, the kinetics and thermodynamics can be extracted, which are comparable to previous fluorescent correlation spectroscopy studies using optical fluorescent resonant energy transfer. By studying the fluctuation amplitude as a function of charge proximity, buffer concentration and solution potential, it is shown that the sensor is based on a field-effect. The sensor has a temporal resolution of 200 μs and a signal to noise ratio of 3-8 when continuously measuring for 30 seconds. By further reducing the parasitics, the sensor has the capabilities to detect biomolecule kinetics down to microsecond resolution, which could make it an attractive tool for single-molecule experiments with fast kinetics

Fundamentals of SARS-CoV-2 Biosensors

COVID-19 diagnostic strategies based on advanced techniques are currently essential topics of interest, with crucial roles in scientific research. This book integrates fundamental concepts and critical analyses that explore the progress of modern methods for the detection of SARS-CoV-2

Novel Analytical Methods in Food Analysis

This reprint provides information on the novel analytical methods used to address challenges occurring at academic, regulatory, and commercial level. All topics covered include information on the basic principles, procedures, advantages, limitations, and applications. Integration of biological reagents, (nano)materials, technologies, and physical principles (spectroscopy and spectrometry) are discussed. This reprint is ideal for professionals of the food industry, regulatory bodies, as well as researchers

Designing Scalable Biological Interfaces

This thesis presents the analysis and design of biological interfacing technologies in light of a need for radical improvements in scalability. It focuses primarily on structural and functional neural data acquisition, but also extends to other problems including genomic editing and nanoscale spatial control. Its main contributions include analysis of the physical limits of large-scale neural recording, experimental development of a screening platform for ion-dependent molecular recording devices, characterization of the design space for molecularly-annotated neural connectomics, and new designs for high-speed genome engineering and bio-nano-fabrication. Articulating governing principles and roadmaps for these domains has contributed to the initiation of multi-institutional projects that are strategically targeted towards scalability

Laboratory Directed Research and Development FY2010 Annual Report

A premier applied-science laboratory, Lawrence Livermore National Laboratory (LLNL) has at its core a primary national security mission - to ensure the safety, security, and reliability of the nation's nuclear weapons stockpile without nuclear testing, and to prevent and counter the spread and use of weapons of mass destruction: nuclear, chemical, and biological. The Laboratory uses the scientific and engineering expertise and facilities developed for its primary mission to pursue advanced technologies to meet other important national security needs - homeland defense, military operations, and missile defense, for example - that evolve in response to emerging threats. For broader national needs, LLNL executes programs in energy security, climate change and long-term energy needs, environmental assessment and management, bioscience and technology to improve human health, and for breakthroughs in fundamental science and technology. With this multidisciplinary expertise, the Laboratory serves as a science and technology resource to the U.S. government and as a partner with industry and academia. This annual report discusses the following topics: (1) Advanced Sensors and Instrumentation; (2) Biological Sciences; (3) Chemistry; (4) Earth and Space Sciences; (5) Energy Supply and Use; (6) Engineering and Manufacturing Processes; (7) Materials Science and Technology; Mathematics and Computing Science; (8) Nuclear Science and Engineering; and (9) Physics