Association of interest, attitude and learning habit in mathematics learning towards enhancing students’ achievement

Mathematics is fundamentally important for Science and Technology, as well as in engineering. Mathematics is compulsory for students since all engineering subjects were Mathematically oriented. However, the preliminary study found that students’ achievement in Mathematics courses have been associated with three main factors, namely interest, attitude and learning habit, as in the KASH Model (Knowledge, Attitude, Skills and Habits). This Model stipulated that poor performance is not just lacking in knowledge and skills but also including poor attitude and habits. Therefore, this study aims to investigate the students’ level and relationship between interest, attitude and learning habit based on KASH Model. A total of 58 students were selected as a sample of the study, who enrolled in the Thermodynamics, Fluid Mechanics and Solid Mechanics subjects. A set of questionnaires with 21 items was used to collect data; a descriptively analysis was used to find the mean and percentage, as well as correlation index using Pearson. The results; high level of factor of interest, attitude and learning habit, and high correlation between interest, attitude and habit. The implication is that teaching and learning process must equally fostering all these variables to achieve a high level of students’ achievement, especially in Mathematics subjects

Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system

Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction. TG2 has been shown to be involved in molecular mechanisms of cancers and several neurodegenerative diseases such as Alzheimer's disease. The present study aimed at cloning and expression of full length human TG2 in Bac-to-Bac baculovirus expression system and evaluation of its activity. Methods: pFastBac HTA donor vector containing coding sequence of human TG2 was constructed. The construct was transformed to DH10Bac for generating recombinant bacmid. The verified bacmid was transfected to insect cell line (Sf9). Expression of recombinant TG2 was examined by RT-PCR, SDS-PAGE and western blot analysis. Functional analysis was evaluated by fluorometric assay and gel electrophoresis. Results: Recombinant bacmid was verified by amplification of a band near to 4500 bp. Expression analysis showed that the enzyme was expressed as a protein with a molecular weight near 80 kDa. Western blot confirmed the presence of TG2 and the activity assays including flurometric assay indicated that the recombinant TG2 was functional. The electrophoresis assay conformed that the expressed TG2 was the indeed capable of crosslinking in the presence of physiological concentration calcium ions. Conclusion: Human TG2 was expressed efficiently in the active biological form in the Bacto- Bac baculovirus expression system. The expressed enzyme could be used for medical diagnostic, or studies which aim at finding novel inhibitors of the enzymes . To best of our knowledge, this is probably the first report of expression of full length human tissue transglutaminase (TG2) using the Bac-to-Bac expression system. © 2016 The Authors

Exosome membrane bound TNF-receptor for the treatment of rheumatoid arthritis

Rheumatoid arthritis (RA) is an autoimmune disease that causes painful inflammation of the synovium of the patient’s joints. However, current treatments for RA have a variety of drawbacks. They often are ineffective, expensive, invasive, risky, cause an immune response, and/ or only provide short term relief. Thus, we developed a new treatment for preventing inflammation: TNF-receptors anchored onto exosome surfaces. Exosomes are nanovesicles that are naturally secreted by most of the cells in our bodies. The many benefits of using exosomes include non-immunogenicity, natural stability in the body, and non-invasiveness. We have demonstrated that exosome membrane bound TNF-receptors have the ability to prevent inflammation in mammalian cells. The success of this project could lead to a clinically effective treatment of rheumatoid arthritis as well as other inflammatory diseases by opening the doors to further research and development of exosomal therapies

Open data and the academy: an evaluation of CKAN for research data management

This paper offers a full and critical evaluation of the open source CKAN software (http://ckan.org) for use as a Research Data Management (RDM) tool within a university environment. It presents a case study of CKAN's implementation and use at the University of Lincoln, UK, and highlights its strengths and current weaknesses as an institutional Research Data Management tool. The author draws on his prior experience of implementing a mixed media Digital Asset Management system (DAM), Institutional Repository (IR) and institutional Web Content Management System (CMS), to offer an outline proposal for how CKAN can be used effectively for data analysis, storage and publishing in academia. This will be of interest to researchers, data librarians, and developers, who are responsible for the implementation of institutional RDM infrastructure. This paper is presented as part of the dissemination activities of the Jisc-funded Orbital project (http://orbital.blogs.lincoln.ac.uk