Petri nets for systems and synthetic biology

We give a description of a Petri net-based framework for modelling and analysing biochemical pathways, which uni¯es the qualita- tive, stochastic and continuous paradigms. Each perspective adds its con- tribution to the understanding of the system, thus the three approaches do not compete, but complement each other. We illustrate our approach by applying it to an extended model of the three stage cascade, which forms the core of the ERK signal transduction pathway. Consequently our focus is on transient behaviour analysis. We demonstrate how quali- tative descriptions are abstractions over stochastic or continuous descrip- tions, and show that the stochastic and continuous models approximate each other. Although our framework is based on Petri nets, it can be applied more widely to other formalisms which are used to model and analyse biochemical networks

A Polynomial Translation of pi-calculus FCPs to Safe Petri Nets

We develop a polynomial translation from finite control pi-calculus processes to safe low-level Petri nets. To our knowledge, this is the first such translation. It is natural in that there is a close correspondence between the control flows, enjoys a bisimulation result, and is suitable for practical model checking.Comment: To appear in special issue on best papers of CONCUR'12 of Logical Methods in Computer Scienc

A Forward Reachability Algorithm for Bounded Timed-Arc Petri Nets

Timed-arc Petri nets (TAPN) are a well-known time extension of the Petri net model and several translations to networks of timed automata have been proposed for this model. We present a direct, DBM-based algorithm for forward reachability analysis of bounded TAPNs extended with transport arcs, inhibitor arcs and age invariants. We also give a complete proof of its correctness, including reduction techniques based on symmetries and extrapolation. Finally, we augment the algorithm with a novel state-space reduction technique introducing a monotonic ordering on markings and prove its soundness even in the presence of monotonicity-breaking features like age invariants and inhibitor arcs. We implement the algorithm within the model-checker TAPAAL and the experimental results document an encouraging performance compared to verification approaches that translate TAPN models to UPPAAL timed automata.Comment: In Proceedings SSV 2012, arXiv:1211.587

Computer-aided analysis of concurrent systems

The introduction of concurrency into programs has added to the complexity of the software design process. This is most evident in the design of communications protocols where concurrency is inherent to the behavior of the system. The complexity exhibited by such software systems makes more evident the needs for computer-aided tools for automatically analyzing behavior.The Distributed Systems project at UCI has been developing a suite of tools, based on Petri nets, which support the design and evaluation of concurrent software systems. This paper focuses attention on one of the tools: the reachability graph analyzer (RGA). This tool provides mechanisms for proving general system properties (e.g., deadlock-freeness) as well as system-specific properties. The tool is sufficiently general to allow a user to apply complex user-defined analysis algorithms to reachability graphs. The alternating-bit protocol with a bounded channel is used to demonstrate the power of the tool and to point to future extensions

Generic Pipelined Processor Modeling and High Performance Cycle-Accurate Simulator Generation

Detailed modeling of processors and high performance cycle-accurate simulators are essential for today's hardware and software design. These problems are challenging enough by themselves and have seen many previous research efforts. Addressing both simultaneously is even more challenging, with many existing approaches focusing on one over another. In this paper, we propose the Reduced Colored Petri Net (RCPN) model that has two advantages: first, it offers a very simple and intuitive way of modeling pipelined processors; second, it can generate high performance cycle-accurate simulators. RCPN benefits from all the useful features of Colored Petri Nets without suffering from their exponential growth in complexity. RCPN processor models are very intuitive since they are a mirror image of the processor pipeline block diagram. Furthermore, in our experiments on the generated cycle-accurate simulators for XScale and StrongArm processor models, we achieved an order of magnitude (~15 times) speedup over the popular SimpleScalar ARM simulator.Comment: Submitted on behalf of EDAA (http://www.edaa.com/

RGA users manual : version 2.3

RGA is an interpreter for a special language designed for the analysis of reachability graphs, or control flow graphs, generated from Petri nets. Although in some cases the reachability graph can become too large to be tractable, or can even be infinite, many interesting problems exist whose reachability graphs are of reasonable size. In RGA, the user has access to the names of the places in the net, and to the states of the reachability graph. The structure of the graph is also available through functions which return the sets of successor or predecessor states of a state and the transition-firings connecting the states. The RGA language allows dynamic typing of identifiers, recursion, and function and operator overloading. Rather than providing a number of predefined analysis functions, RGA provides primitive functions which allow the user to conduct complex analyses with little programming effort. RGA is part of a suite of tools, called P-NUT, intended to facilitate the analysis of concurrent systems described by Petri nets

Read Operators and their Expressiveness in Process Algebras

We study two different ways to enhance PAFAS, a process algebra for modelling asynchronous timed concurrent systems, with non-blocking reading actions. We first add reading in the form of a read-action prefix operator. This operator is very flexible, but its somewhat complex semantics requires two types of transition relations. We also present a read-set prefix operator with a simpler semantics, but with syntactic restrictions. We discuss the expressiveness of read prefixes; in particular, we compare them to read-arcs in Petri nets and justify the simple semantics of the second variant by showing that its processes can be translated into processes of the first with timed-bisimilar behaviour. It is still an open problem whether the first algebra is more expressive than the second; we give a number of laws that are interesting in their own right, and can help to find a backward translation.Comment: In Proceedings EXPRESS 2011, arXiv:1108.407

Encoding Higher Level Extensions of Petri Nets in Answer Set Programming

Answering realistic questions about biological systems and pathways similar to the ones used by text books to test understanding of students about biological systems is one of our long term research goals. Often these questions require simulation based reasoning. To answer such questions, we need formalisms to build pathway models, add extensions, simulate, and reason with them. We chose Petri Nets and Answer Set Programming (ASP) as suitable formalisms, since Petri Net models are similar to biological pathway diagrams; and ASP provides easy extension and strong reasoning abilities. We found that certain aspects of biological pathways, such as locations and substance types, cannot be represented succinctly using regular Petri Nets. As a result, we need higher level constructs like colored tokens. In this paper, we show how Petri Nets with colored tokens can be encoded in ASP in an intuitive manner, how additional Petri Net extensions can be added by making small code changes, and how this work furthers our long term research goals. Our approach can be adapted to other domains with similar modeling needs