Early redox activities modulate Xenopus tail regeneration.

Redox state sustained by reactive oxygen species (ROS) is crucial for regeneration; however, the interplay between oxygen (O2), ROS and hypoxia-inducible factors (HIF) remains elusive. Here we observe, using an optic-based probe (optrode), an elevated and steady O2 influx immediately upon amputation. The spatiotemporal O2 influx profile correlates with the regeneration of Xenopus laevis tadpole tails. Inhibition of ROS production but not ROS scavenging decreases O2 influx. Inhibition of HIF-1α impairs regeneration and stabilization of HIF-1α induces regeneration in the refractory period. In the regeneration bud, hypoxia correlates with O2 influx, ROS production, and HIF-1α stabilization that modulate regeneration. Further analyses reveal that heat shock protein 90 is a putative downstream target of HIF-1α while electric current reversal is a de facto downstream target of HIF-1α. Collectively, the results show a mechanism for regeneration via the orchestration of O2 influx, ROS production, and HIF-1α stabilization

LOCALIZED TEMPORAL PROFILE OF SURVEILLANCE VIDEO

Surveillance videos are recorded pervasively and their retrieval currently still relies on human operators. As an intermediate representation, this work develops a new temporal profile of video to convey accurate temporal information in the video while keeping certain spatial characteristics of targets of interest for recognition. The profile is obtained at critical positions where major target flow appears. We set a sampling line crossing the motion direction to profile passing targets in the temporal domain. In order to add spatial information to the temporal profile to certain extent, we integrate multiple profiles from a set of lines with blending method to reflect the target motion direction and position in the temporal profile. Different from mosaicing/montage methods for video synopsis in spatial domain, our temporal profile has no limit on the time length, and the created profile significantly reduces the data size for brief indexing and fast search of video

Overexpression of hedgehog signaling is associated with epidermal tumor formation in vitamin D receptor-null mice.

The vitamin D receptor (VDR) ligand, 1,25 dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), reduces proliferation and enhances differentiation, and thus has been investigated for a role in preventing or treating cancer. Mice deficient for the VDR display a hyperproliferative response in the hair follicle and epidermis and decreased epidermal differentiation. Unlike their wild-type littermates, when treated with 7,12 dimethylbenzanthracene (DMBA) or UVB, they develop skin tumors, including some characteristic of overexpression of the hedgehog (Hh) pathway. Both the epidermis and utricles of the VDR-null animals overexpress elements of the Hh pathway (sonic hedgehog (Shh) 2.02-fold, patched1 1.58-fold, smoothened 3.54-fold, glioma-associated oncogene homolog (Gli)1 1.17-fold, and Gli2 1.66-fold). This overexpression occurs at an age (11 weeks) at which epidermal hyperproliferation is most visible and is spatially controlled in the epidermis. DMBA- or UVB-induced tumors in the VDR-null mice also overexpress elements of this pathway. Moreover, 1,25(OH)(2)D(3) downregulates the expression of some members of the Hh pathway in an epidermal explants culture system, suggesting a direct regulation by 1,25(OH)(2)D(3). Our results suggest that increased expression of Shh in the keratinocytes of the VDR-null animal activates the Hh pathway, predisposing the skin to the development of both malignant and benign epidermal neoplasms

Development of a Cost-Efficient Multi-Target Classification System Based on FMCW Radar for Security Gate Monitoring

Radar systems have a long history. Like many other great inventions, the origin of radar systems lies in warfare. Only in the last decade, radar systems have found widespread civil use in industrial measurement scenarios and automotive safety applications. Due to their resilience against harsh environments, they are used instead of or in addition to optical or ultrasonic systems. Radar sensors hold excellent capabilities to estimate distance and motion accurately, penetrate non-metallic objects, and remain unaffected by weather conditions. These capabilities make these devices extremely flexible in their applications. Electromagnetic waves centered at frequencies around 24 GHz offer high precision target measurements, compact antenna, and circuitry design, and lower atmospheric absorption than higher frequency-based systems. This thesis studies non-cooperative automatic radar multi-target detection and classification. A prototype of a radar system with a new microwave-radar-based technique for short-range detection and classification of multiple human and vehicle targets passing through a road gate is presented. It allows identifying different types of targets, i.e., pedestrians, motorcycles, cars, and trucks. The developed system is based on a low-cost 24 GHz off-the-shelf FMCW radar, combined with an embedded Raspberry Pi PC for data acquisition and transmission to a remote processing PC, which takes care of detection and classification. This approach, which can find applications in both security and infrastructure surveillance, relies upon the processing of the scattered-field data acquired by the radar. The developed method is based on an ad-hoc processing chain to accomplish the automatic target recognition task, which consists of blocks performing clutter and leakage removal with a frame subtraction technique, clustering with a DBSCAN approach, tracking algorithm based on the \u3b1-\u3b2 filter to follow the targets during traversal, features extraction, and finally classification of targets with a classification scheme based on support vector machines. The approach is validated in real experimental scenarios, showing its capabilities incorrectly detecting multiple targets belonging to different classes (i.e., pedestrians, cars, motorcycles, and trucks). The approach has been validated with experimental data acquired in different scenarios, showing good identification capabilities

Spatial-Temporal Deep Embedding for Vehicle Trajectory Reconstruction from High-Angle Video

Spatial-temporal Map (STMap)-based methods have shown great potential to process high-angle videos for vehicle trajectory reconstruction, which can meet the needs of various data-driven modeling and imitation learning applications. In this paper, we developed Spatial-Temporal Deep Embedding (STDE) model that imposes parity constraints at both pixel and instance levels to generate instance-aware embeddings for vehicle stripe segmentation on STMap. At pixel level, each pixel was encoded with its 8-neighbor pixels at different ranges, and this encoding is subsequently used to guide a neural network to learn the embedding mechanism. At the instance level, a discriminative loss function is designed to pull pixels belonging to the same instance closer and separate the mean value of different instances far apart in the embedding space. The output of the spatial-temporal affinity is then optimized by the mutex-watershed algorithm to obtain final clustering results. Based on segmentation metrics, our model outperformed five other baselines that have been used for STMap processing and shows robustness under the influence of shadows, static noises, and overlapping. The designed model is applied to process all public NGSIM US-101 videos to generate complete vehicle trajectories, indicating a good scalability and adaptability. Last but not least, the strengths of the scanline method with STDE and future directions were discussed. Code, STMap dataset and video trajectory are made publicly available in the online repository. GitHub Link: shorturl.at/jklT0

Airborne photogrammetry and LIDAR for DSM extraction and 3D change detection over an urban area : a comparative study

A digital surface model (DSM) extracted from stereoscopic aerial images, acquired in March 2000, is compared with a DSM derived from airborne light detection and ranging (lidar) data collected in July 2009. Three densely built-up study areas in the city centre of Ghent, Belgium, are selected, each covering approximately 0.4 km(2). The surface models, generated from the two different 3D acquisition methods, are compared qualitatively and quantitatively as to what extent they are suitable in modelling an urban environment, in particular for the 3D reconstruction of buildings. Then the data sets, which are acquired at two different epochs t(1) and t(2), are investigated as to what extent 3D (building) changes can be detected and modelled over the time interval. A difference model, generated by pixel-wise subtracting of both DSMs, indicates changes in elevation. Filters are proposed to differentiate 'real' building changes from false alarms provoked by model noise, outliers, vegetation, etc. A final 3D building change model maps all destructed and newly constructed buildings within the time interval t(2) - t(1). Based on the change model, the surface and volume of the building changes can be quantified

Automatic Classification of Human Epithelial Type 2 Cell Indirect Immunofluorescence Images using Cell Pyramid Matching

This paper describes a novel system for automatic classification of images obtained from Anti-Nuclear Antibody (ANA) pathology tests on Human Epithelial type 2 (HEp-2) cells using the Indirect Immunofluorescence (IIF) protocol. The IIF protocol on HEp-2 cells has been the hallmark method to identify the presence of ANAs, due to its high sensitivity and the large range of antigens that can be detected. However, it suffers from numerous shortcomings, such as being subjective as well as time and labour intensive. Computer Aided Diagnostic (CAD) systems have been developed to address these problems, which automatically classify a HEp-2 cell image into one of its known patterns (eg. speckled, homogeneous). Most of the existing CAD systems use handpicked features to represent a HEp-2 cell image, which may only work in limited scenarios. We propose a novel automatic cell image classification method termed Cell Pyramid Matching (CPM), which is comprised of regional histograms of visual words coupled with the Multiple Kernel Learning framework. We present a study of several variations of generating histograms and show the efficacy of the system on two publicly available datasets: the ICPR HEp-2 cell classification contest dataset and the SNPHEp-2 dataset.Comment: arXiv admin note: substantial text overlap with arXiv:1304.126

Vehicle Detection by Image Processing Using MATLAB:A Colour Based Approach

Due to increasing traffic in the modern times it is imperative to design a system effective in maintaining a record of vehicles passing through a lane or a road. This will help to decrease human interference with the system and result in avoidance of faulty data. Our method would try to focus on detecting cars based on color , so that a proper information about transiting vehicles can be maintained.with the development of color based tracking mechanism tracking of car will become more easier and will lead to further control over the vehicular accident. it has observed that drivers tend to get lethargic and lose focus in such scenarios lead to occurrences of accidents in modern times. hence our mechanism will help to predict the course of vehicle movement and lead to further help so that driver mishaps can be kept in check for road traffic safety and pedestrian protection in the lanes

Investigation of Computer Vision Concepts and Methods for Structural Health Monitoring and Identification Applications

This study presents a comprehensive investigation of methods and technologies for developing a computer vision-based framework for Structural Health Monitoring (SHM) and Structural Identification (St-Id) for civil infrastructure systems, with particular emphasis on various types of bridges. SHM is implemented on various structures over the last two decades, yet, there are some issues such as considerable cost, field implementation time and excessive labor needs for the instrumentation of sensors, cable wiring work and possible interruptions during implementation. These issues make it only viable when major investments for SHM are warranted for decision making. For other cases, there needs to be a practical and effective solution, which computer-vision based framework can be a viable alternative. Computer vision based SHM has been explored over the last decade. Unlike most of the vision-based structural identification studies and practices, which focus either on structural input (vehicle location) estimation or on structural output (structural displacement and strain responses) estimation, the proposed framework combines the vision-based structural input and the structural output from non-contact sensors to overcome the limitations given above. First, this study develops a series of computer vision-based displacement measurement methods for structural response (structural output) monitoring which can be applied to different infrastructures such as grandstands, stadiums, towers, footbridges, small/medium span concrete bridges, railway bridges, and long span bridges, and under different loading cases such as human crowd, pedestrians, wind, vehicle, etc. Structural behavior, modal properties, load carrying capacities, structural serviceability and performance are investigated using vision-based methods and validated by comparing with conventional SHM approaches. In this study, some of the most famous landmark structures such as long span bridges are utilized as case studies. This study also investigated the serviceability status of structures by using computer vision-based methods. Subsequently, issues and considerations for computer vision-based measurement in field application are discussed and recommendations are provided for better results. This study also proposes a robust vision-based method for displacement measurement using spatio-temporal context learning and Taylor approximation to overcome the difficulties of vision-based monitoring under adverse environmental factors such as fog and illumination change. In addition, it is shown that the external load distribution on structures (structural input) can be estimated by using visual tracking, and afterward load rating of a bridge can be determined by using the load distribution factors extracted from computer vision-based methods. By combining the structural input and output results, the unit influence line (UIL) of structures are extracted during daily traffic just using cameras from which the external loads can be estimated by using just cameras and extracted UIL. Finally, the condition assessment at global structural level can be achieved using the structural input and output, both obtained from computer vision approaches, would give a normalized response irrespective of the type and/or load configurations of the vehicles or human loads