2,229 research outputs found
Inferring a Third Spatial Dimension from 2D Histological Images
Histological images are obtained by transmitting light through a tissue
specimen that has been stained in order to produce contrast. This process
results in 2D images of the specimen that has a three-dimensional structure. In
this paper, we propose a method to infer how the stains are distributed in the
direction perpendicular to the surface of the slide for a given 2D image in
order to obtain a 3D representation of the tissue. This inference is achieved
by decomposition of the staining concentration maps under constraints that
ensure realistic decomposition and reconstruction of the original 2D images.
Our study shows that it is possible to generate realistic 3D images making this
method a potential tool for data augmentation when training deep learning
models.Comment: IEEE International Symposium on Biomedical Imaging (ISBI), 201
GAN-based Virtual Re-Staining: A Promising Solution for Whole Slide Image Analysis
Histopathological cancer diagnosis is based on visual examination of stained
tissue slides. Hematoxylin and eosin (H\&E) is a standard stain routinely
employed worldwide. It is easy to acquire and cost effective, but cells and
tissue components show low-contrast with varying tones of dark blue and pink,
which makes difficult visual assessments, digital image analysis, and
quantifications. These limitations can be overcome by IHC staining of target
proteins of the tissue slide. IHC provides a selective, high-contrast imaging
of cells and tissue components, but their use is largely limited by a
significantly more complex laboratory processing and high cost. We proposed a
conditional CycleGAN (cCGAN) network to transform the H\&E stained images into
IHC stained images, facilitating virtual IHC staining on the same slide. This
data-driven method requires only a limited amount of labelled data but will
generate pixel level segmentation results. The proposed cCGAN model improves
the original network \cite{zhu_unpaired_2017} by adding category conditions and
introducing two structural loss functions, which realize a multi-subdomain
translation and improve the translation accuracy as well. % need to give
reasons here. Experiments demonstrate that the proposed model outperforms the
original method in unpaired image translation with multi-subdomains. We also
explore the potential of unpaired images to image translation method applied on
other histology images related tasks with different staining techniques
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