The Band Excitation Method in Scanning Probe Microscopy for Rapid Mapping of Energy Dissipation on the Nanoscale

Mapping energy transformation pathways and dissipation on the nanoscale and understanding the role of local structure on dissipative behavior is a challenge for imaging in areas ranging from electronics and information technologies to efficient energy production. Here we develop a novel Scanning Probe Microscopy (SPM) technique in which the cantilever is excited and the response is recorded over a band of frequencies simultaneously rather than at a single frequency as in conventional SPMs. This band excitation (BE) SPM allows very rapid acquisition of the full frequency response at each point (i.e. transfer function) in an image and in particular enables the direct measurement of energy dissipation through the determination of the Q-factor of the cantilever-sample system. The BE method is demonstrated for force-distance and voltage spectroscopies and for magnetic dissipation imaging with sensitivity close to the thermomechanical limit. The applicability of BE for various SPMs is analyzed, and the method is expected to be universally applicable to all ambient and liquid SPMs.Comment: 32 pages, 9 figures, accepted for publication in Nanotechnolog

Remote Cell Growth Sensing Using Self-Sustained Bio-Oscillations

A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable “biological” oscillator. The system enables the remote acquisition and management of the “biological” oscillation signals through a secure web interface. The indirectly observed biological properties are cell growth and cell number, which are straightforwardly related to the measured bio-oscillation signal parameters, i.e., frequency and amplitude. The sensor extracts the information without complex circuitry for acquisition and measurement, taking advantage of the microcontroller features. A discrete prototype for sensing and remote monitoring is presented along with the experimental results obtained from the performed measurements, achieving the expected performance and outcomes

Frequency-splitting Dynamic MRI Reconstruction using Multi-scale 3D Convolutional Sparse Coding and Automatic Parameter Selection

Department of Computer Science and EngineeringIn this thesis, we propose a novel image reconstruction algorithm using multi-scale 3D con- volutional sparse coding and a spectral decomposition technique for highly undersampled dy- namic Magnetic Resonance Imaging (MRI) data. The proposed method recovers high-frequency information using a shared 3D convolution-based dictionary built progressively during the re- construction process in an unsupervised manner, while low-frequency information is recovered using a total variation-based energy minimization method that leverages temporal coherence in dynamic MRI. Additionally, the proposed 3D dictionary is built across three different scales to more efficiently adapt to various feature sizes, and elastic net regularization is employed to promote a better approximation to the sparse input data. Furthermore, the computational com- plexity of each component in our iterative method is analyzed. We also propose an automatic parameter selection technique based on a genetic algorithm to find optimal parameters for our numerical solver which is a variant of the alternating direction method of multipliers (ADMM). We demonstrate the performance of our method by comparing it with state-of-the-art methods on 15 single-coil cardiac, 7 single-coil DCE, and a multi-coil brain MRI datasets at different sampling rates (12.5%, 25% and 50%). The results show that our method significantly outper- forms the other state-of-the-art methods in reconstruction quality with a comparable running time and is resilient to noise.ope

Measuring frequency fluctuations in nonlinear nanomechanical resonators

Advances in nanomechanics within recent years have demonstrated an always expanding range of devices, from top-down structures to appealing bottom-up MoS$_2$ and graphene membranes, used for both sensing and component-oriented applications. One of the main concerns in all of these devices is frequency noise, which ultimately limits their applicability. This issue has attracted a lot of attention recently, and the origin of this noise remains elusive up to date. In this Letter we present a very simple technique to measure frequency noise in nonlinear mechanical devices, based on the presence of bistability. It is illustrated on silicon-nitride high-stress doubly-clamped beams, in a cryogenic environment. We report on the same $T/f$ dependence of the frequency noise power spectra as reported in the literature. But we also find unexpected {\it damping fluctuations}, amplified in the vicinity of the bifurcation points; this effect is clearly distinct from already reported nonlinear dephasing, and poses a fundamental limit on the measurement of bifurcation frequencies. The technique is further applied to the measurement of frequency noise as a function of mode number, within the same device. The relative frequency noise for the fundamental flexure $\delta f/f_0$ lies in the range $0.5 - 0.01~$ppm (consistent with literature for cryogenic MHz devices), and decreases with mode number in the range studied. The technique can be applied to {\it any types} of nano-mechanical structures, enabling progresses towards the understanding of intrinsic sources of noise in these devices.Comment: Published 7 may 201

Noise Measurement Setup for Quartz Crystal Microbalance

Quartz crystal microbalance (QCM) is a high sensitive chemical sensor which has found widespread spectrum of applications. There are several mechanisms that are related to fluctuation phenomena. Since the aim of our research is oriented to study the sensitivity and influence of different kind of noises on sensor resolution, we modified an existing method to measure the small frequency fluctuation of QCM. The paper describes our measurement setup, in which a quartz crystal oscillator with coated active layers and a reference quartz oscillator are driven by two oscillator circuits. Each one regulates a frequency of a crystal at the minimum impedance which corresponds to the series resonance. A data-acquisition card triggers on the rise-edges of the output signal and stores these corresponding times on which the instantaneous frequency is estimated by own-written software. In comparison to other measurement setups, our approach can acquire immediate change of QCM frequency, thus, chemical processes can be even described on the basis of high-order statistics. The experiments were provided on quartz crystals with the sorption layer of polypyrrole, which is suitable for the construction of QCM humidity sensors

Generation of one-million-mode continuous-variable cluster state by unlimited time-domain multiplexing

In recent quantum optical continuous-variable experiments, the number of fully inseparable light modes has drastically increased by introducing a multiplexing scheme either in the time domain or in the frequency domain. Here, modifying the time-domain multiplexing experiment reported in Nature Photonics 7, 982 (2013), we demonstrate successive generation of fully inseparable light modes for more than one million modes. The resulting multi-mode state is useful as a dual-rail CV cluster state. We circumvent the previous problem of optical phase drifts, which has limited the number of fully inseparable light modes to around ten thousands, by continuous feedback control of the optical system.Comment: 12 pages, 8 figure

Prototyping and Experimentation of a Closed-Loop Wireless Power Transmission with Channel Acquisition and Waveform Optimization

A systematic design of adaptive waveform for Wireless Power Transfer (WPT) has recently been proposed and shown through simulations to lead to significant performance benefits compared to traditional non-adaptive and heuristic waveforms. In this study, we design the first prototype of a closed-loop wireless power transfer system with adaptive waveform optimization based on Channel State Information acquisition. The prototype consists of three important blocks, namely the channel estimator, the waveform optimizer, and the energy harvester. Software Defined Radio (SDR) prototyping tools are used to implement a wireless power transmitter and a channel estimator, and a voltage doubler rectenna is designed to work as an energy harvester. A channel adaptive waveform with 8 sinewaves is shown through experiments to improve the average harvested DC power at the rectenna output by 9.8% to 36.8% over a non-adaptive design with the same number of sinewaves.Comment: accepted for publication in IEEE WPTC 201

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Förster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data.

The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and through continuous dynamic rearrangements, regulates genome function [1]. However, given that nucleosome packaging occurs on a spatial scale well below the diffraction limit, real time observation of chromatin structure in live cells by optical microscopy has proved technically difficult, despite recent advances in live cell super resolution imaging [2]. One alternative solution to quantify chromatin structure in a living cell at the level of nucleosome proximity is to measure and spatially map Förster resonance energy transfer (FRET) between fluorescently labelled histones - the core protein of a nucleosome [3]. In recent work we established that the phasor approach to fluorescence lifetime imaging microscopy (FLIM) is a robust method for the detection of histone FRET which can quantify nuclear wide chromatin compaction in the presence of cellular autofluorescence [4]. Here we share FLIM data recording histone FRET in live cells co-expressing H2B-eGFP and H2B-mCherry. The data was acquired in the frequency domain [5] and processed by the phasor approach to lifetime analysis [6]. The data can be valuable to researchers interested in using the histone FRET assay since it highlights the impact of cellular autofluorescence and acceptor-donor ratio on quantifying chromatin compaction. The data is related to the research article "Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response" [4]

Compressive Sensing for Spectroscopy and Polarimetry

We demonstrate through numerical simulations with real data the feasibility of using compressive sensing techniques for the acquisition of spectro-polarimetric data. This allows us to combine the measurement and the compression process into one consistent framework. Signals are recovered thanks to a sparse reconstruction scheme from projections of the signal of interest onto appropriately chosen vectors, typically noise-like vectors. The compressibility properties of spectral lines are analyzed in detail. The results shown in this paper demonstrate that, thanks to the compressibility properties of spectral lines, it is feasible to reconstruct the signals using only a small fraction of the information that is measured nowadays. We investigate in depth the quality of the reconstruction as a function of the amount of data measured and the influence of noise. This change of paradigm also allows us to define new instrumental strategies and to propose modifications to existing instruments in order to take advantage of compressive sensing techniques.Comment: 11 pages, 9 figures, accepted for publication in A&

Contribution to Efficient Use of Narrowband Radio Channel

Předkládaná práce se soustředí na problematiku využívání úzkopásmového rádiového kanálu rádiovými modemy, které jsou určené pro průmyslové aplikace pozemní pohyblivé rádiové služby, specifikované v dominantní míře Evropským standardem ETSI EN 300 113. Tato rádiová zařízení se používají v kmitočtových pásmech od 30 MHz do 1 GHz s nejčastěji přidělovanou šířkou pásma 25 kHz a ve většině svých instalací jsou využívána ve fixních nebo mobilních bezdrátových sítích. Mezi typické oblasti použití patří zejména datová telemetrie, aplikace typu SCADA, nebo monitorování transportu strategických surovin. Za hlavní znaky popisovaného systému lze označit komunikační pokrytí značných vzdáleností, dané především vysokou výkonovou účinnosti datového přenosu a využívaní efektivních přístupových technik na rádiový kanál se semiduplexním komunikačním režimem. Striktní požadavky na elektromagnetickou kompatibilitu umožňují těmto zařízením využívat spektrum i v oblastech kmitočtově blízkým jiným komunikačním systémům bez nutnosti vkládání dodatečných ochranných frekvenčních pásem. Úzkopásmové rádiové komunikační systémy, v současnosti používají převážně exponenciální digitální modulace s konstantní modulační obálkou zejména z důvodů velice striktních omezení pro velikost výkonu vyzářeného do sousedního kanálu. Dosahují tak pouze kompromisních hodnot komunikační účinnosti. Úpravy limitů příslušných rádiových parametrů a rychlý rozvoj prostředků číslicového zpracování signálu v nedávné době, dnes umožňují ekonomicky přijatelné využití spektrálně efektivnějších modulačních technik i v těch oblastech, kde je prioritní využívání úzkých rádiových kanálů. Cílem předkládané disertační práce je proto výzkum postupů směřující ke sjednocení výhodných vlastností lineárních a nelineárních modulací v moderní konstrukci úzkopásmového rádiového modemu. Účelem tohoto výzkumu je efektivní a „ekologické“ využívání přidělené části frekvenčního spektra. Mezi hlavní dílčí problémy, jimiž se předkládaná práce zabývá, lze zařadit zejména tyto: Nyquistova modulační filtrace, navrhovaná s ohledem na minimalizaci nežádoucích elektromagnetických interferencí, efektivní číslicové algoritmy frekvenční demodulace a rychlé rámcové a symbolové synchronizace. Součástí práce je dále analýza navrhovaného řešení z pohledu celkové konstrukce programově definovaného rádiového modemu v rovině simulací při vyšetřování robustnosti datového přenosu rádiovým kanálem s bílým Gaussovským šumem nebo kanálem s únikem v důsledku mnohacestného šíření signálu. Závěr práce je pak zaměřen na prezentování výsledků praktické části projektu, v níž byly testovány, měřeny a analyzovány dvě prototypové konstrukce rádiového zařízení. Tato finální část práce obsahuje i praktická doporučení, vedoucí k vyššímu stupni využitelnosti spektrálně efektivnějších komunikačních režimů v oblasti budoucí generace úzkopásmových zařízení pozemní pohyblivé rádiové služby.he industrial narrowband land mobile radio (LMR) devices, as considered in this dissertation project, has been subject to European standard ETSI EN 300 113. The system operates on frequencies between 30 MHz and 1 GHz, with channel separations of up to 25 kHz, and is intended for private, fixed, or mobile, radio packet switching networks. Data telemetry, SCADA, maritime and police radio services; traffic monitoring; gas, water, and electricity producing factories are the typical system applications. Long distance coverage, high power efficiency, and efficient channel access techniques in half duplex operation are the primary advantages the system relays on. Very low level of adjacent channel power emissions and robust radio receiver architectures, with high dynamic range, enable for a system’s coexistence with various communication standards, without the additional guard band frequency intervals. On the other hand, the strict limitations of the referenced standard as well as the state of the technology, has hindered the increase in communication efficiency, with which the system has used its occupied bandwidth. New modifications and improvements are needed to the standard itself and to the up-to-date architectures of narrowband LMR devices, to make the utilization of more efficient modes of system operation practically realizable. The main objective of this dissertation thesis is therefore to find a practical way how to combine the favorable properties of the advanced nonlinear and linear digital modulation techniques in a single digital modem solution, in order to increase the efficiency of the narrowband radio channel usage allocated to the new generation of the industrial LMR devices. The main attention is given to the particular areas of digital modem design such as proposal of the new family of the Nyquist filters minimizing the adjacent channel interference, design and analysis of the efficient algorithms for frequency discrimination, fast frame and symbol