Advanced Process Monitoring for Industry 4.0

This book reports recent advances on Process Monitoring (PM) to cope with the many challenges raised by the new production systems, sensors and “extreme data” conditions that emerged with Industry 4.0. Concepts such as digital-twins and deep learning are brought to the PM arena, pushing forward the capabilities of existing methodologies to handle more complex scenarios. The evolution of classical paradigms such as Latent Variable modeling, Six Sigma and FMEA are also covered. Applications span a wide range of domains such as microelectronics, semiconductors, chemicals, materials, agriculture, as well as the monitoring of rotating equipment, combustion systems and membrane separation processes

Spatial Distribution of Meso- and Microplastics in Floodplain Soilscapes: Novel Insights from Rural to Urban Floodplains in Central Germany

Plastics and especially microplastics have become an emerging threat to global ecosystems. Despite the manifold benefits and applications of the human-made material plastic, the uncontrolled release of plastics into the environment has led to a “global plastic crisis”. During the last decades it becomes apparent that this crisis leads to the presence of plastics within different environments including marine, aquatic and terrestrial systems under worldwide evidence. Furthermore, environmental plastic research was able to reveal that although plastic often ends up in oceans, the majority of plastics in the environment are transported as part of a “global plastic cycle” from the land to sea via river systems. Those river systems are not isolated in the landscape, but rather a part of an “aquatic-terrestrial interface” which also encompasses floodplains and their soilscapes. The present thesis focuses on the spatial distribution and spatio-temporal accumulation of meso- and microplastics in floodplain soilscapes following the overall objective to unravel the role of floodplain soilscapes as depositional areas of plastics within the global plastic cycle. In this context, a number of individual contributions have been published, reaching from conceptual spatial research approaches, over case studies conducted within two different floodplain soilscapes, to further opinions on the scientific benefit of plastic residues in floodplain soils. The individual contributions are linked by the major hypothesis that floodplain soilscapes act as temporal accumulation sites for plastics, driven by flood-related processes and land use over the last 70 years. To proof this major hypothesis and to overcome the lack of spatial reference in microplastics research, a geospatial sampling approach was conducted. Initial spatial data on meso- and microplastics in floodplain soils were obtained by a holistic analysis approach including the analysis of basic soil feature and metal analysis, the quantification of meso- and microplastics as well as sediment dating. Within both studied river floodplains geospatial sampling enables a detection of meso- and microplastics over the entire floodplain area and within the entire soil column reaching depths of two meters. Additionally, a frequent accumulation of plastics was found within the upper 50 cm of floodplain soils. In combination with dating of near-channel floodplain sites, it could be demonstrated that those plastic accumulations are related to recent sedimentary deposits since the 1960s. However, evidence of plastic from deeper soil layers suggests that vertical displacements in floodplain soils occur and that plastics become mobilized. Furthermore, the presence of plastics in upstream areas suggests that plastics are released to river systems and deposited via flood dynamics already in rural areas. Additionally it appears that anthropogenic impacts, such as tillage or floodplain restoration influence plastic distributions. The findings of this thesis clarify that floodplain soilscapes are part of the global plastic cycle as temporally depositional areas of plastics, but raising further questions on the mobility of plastics in soils and about the exact contribution of different environmental drivers towards plastic deposition. Finally, the present thesis indicates that the spatial reference of environmental plastic research should be rethought, in order to understand the spatial dynamics of plastics within the aquatic-terrestrial interface

An ultra-high throughput mutational spectrometer for human genetic diagnostics

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.Includes bibliographical references (p. 221-243).Discovering the genetic causes of common diseases may require scanning for mutations in all of the genes in a million people, a significant undertaking. Such discoveries would revolutionize biotechnology, potentially enabling simple genetic tests for risk and targeted preventative or therapeutic strategies. An increase in throughput of genetic analysis instrumentation by several orders of magnitude is essential to undertake such an ambitious task. In this thesis, progress will be presented towards the creation of such a "mutational spectrometer" instrument containing up to 10,000 capillary channels and enabled with subsystems for loading, separating, and detecting fluorescently-labeled DNA. Challenges include DNA manipulation, optical signal detection, macro/micro design integration, precision alignment and assembly, and thermal control. To manipulate DNA, we have utilized a bioMEMS design platform for interfacing to an array of separation channels that enables electrokinetic biomolecule loading, detection, and fraction collection in independent wells.(cont.) Signal detection is accomplished by a sensitive (107 molecule limit-of-detection), scalable (to 10,000 independent channels), end-of-column fluorescence detection technology that accommodates tightly packed capillary arrays as required for ultra-high throughput electrophoretic separation. Capillary array assembly and constraint technologies have been developed for 2-D arrays containing as many as 10,000 replaceable capillaries. Thermal control requirements of 0.3 °C over the entire 10,000 channel array are met with a cross-flow water heat exchanger. Additional subsystems for forcing a viscous polymer matrix into the capillaries, and interfacing the capillary array to a fluid reservoir for electrophoresis have also been developed, as required. This work lays the foundation for the realization of a mutational spectrometer instrument that will enable population-wide pangenomic analyses to uncover the genetic causes of common diseases.by Craig Richard Forest.Ph.D