Influence of trace erythromycin and eryhthromycin-H2O on carbon and nutrients removal and on resistance selection in sequencing batch reactors (SBRs).

Three sequencing batch reactors (SBRs) were operated in parallel to study the effects of trace erythromycin (ERY) and ERY-H2O on the treatment of a synthetic wastewater. Through monitoring (1) daily effluents and (2) concentrations of nitrogen (N) and phosphorous (P) in certain batch cycles of the three reactors operated from transient to steady states, the removal of carbon, N, and P was affected negligibly by ERY (100 microg/L) or ERY-H2O (50 microg/L) when compared with the control reactor. However, through analyzing microbial communities of the three steady state SBRs on high-density microarrays (Phylo-Chip), ERY, and ERY-H2O had pronounced effects on the community composition of bacteria related to N and P removal, leading to diversity loss and abundance change. The above observations indicated that resistant bacteria were selected upon exposure to ERY or ERY-H2O. Shortterm batch experiments further proved the resistance and demonstrated that ammonium oxidation (56-95%) was inhibited more significantly than nitrite oxidation (18-61%) in the presence of ERY (100, 400, or 800 microg/L). Therefore, the presence of ERY or ERY-H2O (at microg/L levels) shifted the microbial community and selected resistant bacteria, which may account for the negligible influence of the antibiotic ERY or its derivative ERY-H2O (at microg/L levels) on carbon, N, and P removal in the SBRs

Innovative Surveillance and Process Control in Water Resource Recovery Facilities

Water Resource Recovery Facilities (WRRF), previously known as Wastewater Treatment Plants (WWTP), are getting increasingly complex, with the incorporation of sludge processing and resource recovery technologies. Along with maintaining a stringent effluent water quality standard, the focus is gradually shifting towards energy-efficient operations and recovery of resources. The new objectives of the WRRF demand an economically optimal operation of processes that are subjected to extreme variations in flowrate and composition at the influent. The application of online monitoring, process control, and automation in WRRF has already shown a steady increase in the past decade. However, the advanced model-based optimal control strategies, implemented in most process industries, are less common in WRRF. The complex nature of biological processes, the unavailability of simplified process models, and a lack of cost-effective surveillance infrastructure have often hindered the implementation of advanced control strategies in WRRF. The ambition of this research is to implement and validate cost-efficient monitoring alternatives and advanced control strategies for WRRF by fully utilizing the powerful Internet of Things (IoT) and data science tools. The first step towards implementing an advanced control strategy is to ensure the availability of surveillance infrastructure for monitoring nutrient compositions in WRRF processes. In Paper A, a soft sensor, based on Extended Kalman Filter, is developed for estimating water-quality parameters in a Sequential Batch MBBR process using reliable and inexpensive online sensors. The model used in the soft sensor combines the mechanistic understanding of the nutrient removal process with a statistical correlation between nutrient composition and easy-to-measure parameters. Paper B demonstrates the universality of the soft sensor through validation tests conducted in a Continuous Multistage MBBR pilot plant. The drift in soft-sensor estimation caused by a mismatch between the mathematical model and process behavior is studied in Paper B. The robustness of the soft sensor is assessed by observing estimated nutrient composition values for a period of three months. A systematic method to calibrate the measurement model and update model parameters using data from periodic lab measurements are discussed in Paper B. The term SCADA has been ubiquitous while mentioning online monitoring and control strategy deployment in WRRFs. The present digital world of affordable communication hardware, compact single board processors, and high computational power presents several options for remote monitoring and deployment of soft sensors. In Paper C, a cost-effective IoT strategy is developed by using an open-source programming language and inexpensive hardware. The functionalities of the IoT infrastructure are demonstrated by using it to deploy a soft sensor script in the ContinuousMultistage MBBR pilot plant. A cost-comparison between the commercially available alternatives presented in Paper A and the open-source IoT strategy in Paper B and Paper C highlights the benefits of the new monitoring infrastructure. Lack of reliable control models have often been the cause for the poor performance of advanced control strategies, such as Model Predictive Controls (MPC) when implemented to complex biological nutrient removal processes. Paper D attempts to overcome the inadequacies of the linear prediction model by combining a recursive model parameter estimator with the linear MPC. The new MPC variant, called the adaptive MPC (AMPC), reduces the dependency of MPC on the accuracy of its prediction model. The performance of the AMPC is compared with that of a linear MPC, nonlinear MPC, and the traditional proportional-integral cascade control through simulator-based evaluations conducted on the Benchmark Simulator platform(BSM2). The advantages of AMPC compared to its counterparts, in terms of reducing the aeration energy, curtailing the number of effluent ammonia violations, and the use of computational resources, are highlighted in Paper D. The complex interdependencies between different processes in a WRRF pose a significant challenge in defining constant reference points for WRRFs operations. A strategy that decides control outputs based on economic parameters rather than maintaining a fixed reference set-point is introduced in Paper E. The model-based control strategy presented in Paper D is further improved by including economic parameters in the MPC’s objective function. The control strategy known as Economic MPC (EMPC) is implemented for optimal dosage of magnesium hydroxide in a struvite recovery unit installed in a WRRF. A comparative study performed on the BSM2 platform demonstrates a significant improvement in overall profitability for the EMPC when compared to a constant or a feed-forward flow proportional control strategy. The resilience of the EMPC strategy to variations in the market price of struvite is also presented in Paper E. A combination of cost-effective monitoring infrastructure and advanced control strategies using advanced IoTs and data science tools have been documented to overcome some of the critical problems encountered in WRRFs. The overall improvement in process efficiency, reduction in operating costs, an increase in resource recovery, and a substantial reduction in the price of online monitoring infrastructure contribute to the overall aim of transitioning WRRFs to a self-sustaining facility capable of generating value-added products.Water Resource Recovery Facilities (WRRF), tidligere kjent som avløpsrenseanlegg (WWTP), blir stadig mer komplekse ettersom flere prosess steg tillegges anleggene i form av slambehandling og ressursgjenvinningsteknologi. Foruten hovedmålet om å imøtekomme strenge avløpsvannskvalitetskrav, har anleggenes fokus gradvis skiftet mot energieffektiv drift og gjenvinning av ressurser. Slike nye mål krever økonomisk optimal drift av prosesser som er utsatt for ekstreme variasjoner i volum og sammensetning av tilløp. Bruk av online overvåking, prosesskontroll og automatisering i WRRF har jevnt økt det siste tiåret. Likevel er avanserte modellbaserte kontrollstrategier for optimalisering ikke vanlig i WRRF, i motsetning til de fleste prosessindustrier. Komplekse forhold i biologiske prosesser, mangel på tilgang til pålitelige prosessmodeller og mangel på kostnadseffektiv overvåkingsinfrastruktur har ofte hindret implementeringen av avanserte kontrollstrategier i WRRF. Ambisjonen med denne avhandlingen er å implementere og validere kostnadseffektive overvåkingsalternativer og avanserte kontrollstrategier somutnytter kraftige Internet of Things (IoT) og datavitenskapelige verktøy i WRRF sammenheng. Det første steget mot implementering av en avansert kontrollstrategi er å sørge for tilgjengelighet av overvåkingsinfrastruktur for måling av næringsstoffer i WRRF-prosesser. Paper A demonstrerer en virtuell sensor basert på et utvidet Kalman filter, utviklet for å estimere vannkvalitetsparametere i en sekvensiell batch MBBR prosess ved hjelp av pålitelige og rimelige online sensorer. Modellen som brukes i den virtuelle sensoren kombinerer en mekanistisk forståelse av prosessen for fjerning av næringsstoffer fra avløpsvann med et statistisk sammenheng mellom næringsstoffsammensetning i avløpsvann og parametere som er enkle å måle. Paper B demonstrerer det universale bruksaspektet til den virtuelle sensoren gjennom valideringstester utført i et kontinuerlig flertrinns MBBR pilotanlegg. Feilene i sensorens estimering forårsaket av uoverensstemmelse mellom den matematiske modellen og prosesseatferden er undersøkt i Paper B. Robustheten til den virtuelle sensoren ble vurdert ved å observere estimerte næringssammensetningsverdier i en periode på tre måneder. En systematisk metode for å kalibrere målemodellen og oppdatere modellparametere ved hjelp av data fra periodiske laboratoriemålinger er diskutert i Paper B. Begrepet SCADA har alltid vært til stede når online overvåking og kontrollstrategi innen WRRF er nevnt. Den nåværende digitale verdenen med god tilgjengelighet av rimelig kommunikasjonsmaskinvare, kompakte enkeltkortprosessorer og høy beregningskraft presenterer flere muligheter for fjernovervåking og implementering av virtuelle sensorer. Paper C viser til utvikling av en kostnadseffektiv IoT-strategi ved hjelp av et programmeringsspråk med åpen kildekode og rimelig maskinvare. Funksjonalitetene i IoT-infrastruktur demonstreres gjennom implementering av et virtuelt sensorprogram i et kontinuerlig flertrinns MBBR pilotanlegg. En kostnadssammenligning mellom de kommersielt tilgjengelige alternativene som presenteres i Paper A og åpen kildekode-IoT-strategi i Paper B og Paper C fremhever fordelene med den nye overvåkingsinfrastrukturen. Mangel på pålitelige kontrollmodeller har ofte vært årsaken til svake resultater i avanserte kontrollstrategier, som for eksempel Model Predictive Control (MPC) når de implementeres i komplekse biologiske prosesser for fjerning av næringsstoffer. Paper D prøver å løse manglene i MPC ved å kombinere en rekursiv modellparameterestimator med lineær MPC. Den nye MPC-varianten, kalt Adaptiv MPC (AMPC), reduserer MPCs avhengighet av nøyaktigheten i prediksjonsmodellen. Ytelsen til AMPC sammenlignes med ytelsen til en lineær MPC, ikke-lineær MPC og tradisjonell proportionalintegral kaskadekontroll gjennom simulatorbaserte evalueringer utført på Benchmark Simulator plattformen (BSM2). Fordelene med AMPC sammenlignet med de andre kontrollstrategiene er fremhevet i Paper D og demonstreres i sammenheng redusering av energibruk ved lufting i luftebasseng, samt redusering i antall brudd på utslippskrav for ammoniakk og bruk av beregningsressurser. De komplekse avhengighetene mellom forskjellige prosesser i en WRRF utgjør en betydelig utfordring når man skal definere konstante referansepunkter for WRRF under drift. En strategi som bestemmer kontrollsignaler basert på økonomiske parametere i stedet for å opprettholde et fast referansesettpunkt introduseres i Paper E. Den modellbaserte kontrollstrategien fra PaperDforbedres ytterligere ved å inkludere økonomiske parametere iMPCs objektiv funksjon. Denne kontrollstrategien kalles Economic MPC (EMPC) og er implementert for optimal dosering av magnesiumhydroksid i en struvit utvinningsenhet installert i en WRRF. En sammenligningsstudie utført på BSM2 plattformen viste en betydelig forbedring i den totale lønnsomheten ved bruk av EMPC sammenlignet med en konstant eller en flow proportional kontrollstrategi. Robustheten til EMPC-strategien for variasjoner i markedsprisen på struvit er også demonstrert i Paper E. En kombinasjon av kostnadseffektiv overvåkingsinfrastruktur og avanserte kontrollstrategier ved hjelp av avansert IoT og datavitenskapelige verktøy er brukt for å løse flere kritiske utfordringer i WRRF. Den samlede forbedringen i prosesseffektivitet, reduksjon i operasjonskostnader, økt ressursgjenvinning og en betydelig reduksjon i pris for online overvåkningsinfrastruktur bidrar til det overordnede målet om å gå over til bærekraftige WRRF som er i stand til å generere verdiskapende produkter.DOSCON A

Control and identification in activated sludge processes = Regeling en identifikatie in aktief-slib processen

This thesis is about control and identification in activated sludge processes (ASP's). The chapters in this thesis are divided in two parts. Part I deals with the development of the best feasible, close-to-optimal adaptive receding horizon optimal controller (RHOC) for N-removal in a continuously mixed alternating activated sludge process reactor. Subsequently this controller and the most common existing controllers are mutually compared by means of simulations. In addition the application of the close-to-optimal RHOC controller to a system of two hydraulically connected alternating reactors is simulated for a range of plant designs within this class. In this way the combination of design and operation is optimized. Part II concerns identification on the basis of DO-measurements and respirometry. First the DO-dynamics in a continuously mixed ASP reactor are identified, including the non-linear relation between k L a and q air . Subsequenly the dynamics of a (DO-sensor based) continuous flow respirometer are identified by exciting its dynamics.In chapter 1 the principles of the N-removing ASP are shortly explained. The new problem of total-N removal is discussed. The general features of the ASP control problem are listed: disturbance attenuation, storm events, process uncertainty and variation, multiple time-scales. Special attention is paid to the potential of RHOC. The literature with respect to operational aspects of N-removal as well as the use of DO-sensors and respirometers in ASP operation is coarsely reviewed. It is argued that the anoxic periods approach for N-removal offers two principle advantages over the anoxic zones approach: excitation of dynamics and no need for internal recirculation. Some problems in the field are indicated. With respect to DO-sensors it is illustrated that the challenges today are in the field of extracting not only DO but also additional information from its readings. All experiments in this thesis have been carried out at a pilot scale ASP. A description of this pilot plant is given in chapter 1. The chapter ends with the formulation of research objectives and the thesis outline.Chapters 2 till 5 present the design procedure for the adaptive RHOC for control of NH 4 and NO x , though not exactly chronologically. The first step is presented in chapter 4, it concerns application of optimal control to the N-removal part of the generally accepted Activated Sludge Model no. 1. From this optimal control study it occurs that alternating nitrification/denitrification, as opposed to simultaneous nitrification/denitrification, may be optimal indeed. This, together with the risk of sludge bulking at limiting DO-values, justifies the limitation to alternating process operation. To implement an optimal control strategy on-line the receding horizon principle is needed, leading to RHOC. RHOC uses an internal process model for short term predictions. Hence a computationally efficient process model is required. Such a model is developed in chapter 2 by capturing the slower process dynamics in time-varying model parameters. It is taken into account that the model structure must be suited for recursive identification of the time-varying model parameters from the measurements.RHOC, like any model predictive controller, computes the current controls on the basis of model predictions upto horizon H . Hence the sum of squared 1, 2, .., H -step ahead prediction errors is a natural identification criterion. In chapter 2 this idea is postulated and applied to NH 4 /NO x measurements collected from the pilot scale ASP described in chapter 1. H appears to affect the parameter estimates significantly, supporting the idea that use of this new identification criterion will improve MPC performance in general.In chapter 4 RHOC with this simple model is applied to the pilot plant's alternating reactor. The controller successfully passed several tests, but it also appeared that the performance of this controller is suboptimal due to inaccurate model predictions. This was to be expected, as the simplicity of the N-removal model in chapter 2 has been achieved by capturing the slower process dynamics in the model parameters, while in this stage they are not recursively estimated.The results of chapter 4 illustrate that recursive identification of (some of the) model parameters is required to keep the model uptodate. Chapter 3 presents the algorithm for recursive identification of those model parameters. The Kalman filter is used, because it has the attractive feature that the filter gain accompanying non-identifiable parameters ( e.g. the nitrification rate during anoxic periods) increases linearly in time. It is proven that this increase of the filter gain will not cause instability during normal process operation. The method performs excellently on real data.Chapter 5 concerns adaptive RHOC of N-removal in alternating ASP reactors, being the combination of the recursively identified model in chapter 3 and the RHOC controller in chapter 4. Although stability of the nonlinear RHOC feedback controller has not been proven, not to mention its combination with recursive identification, only one source of instability was encountered in many experiments. This is the scenario in which NH 4 dominates the objective functional, its setpoint is zero and the estimated rate of nitrification has become negative for whatever reason. In that case the controller will keep aeration off to prevent the predicted production of NH 4 , as a consequence no new information to update the estimated nitrification rate is obtained and the deadlock is there. Obviously this scenario is easy to prevent and does not occur under normal operating conditions.In chapter 4 the unusual observation is done that the RHOC performance is nearly invariant to its prediction horizon. This triggered a study on the cause of this phenomena and an effort to generalize the results as far as possible, the results are presented in chapter 6. It has led to the derivation of an l 1 -norm optimal state feedback controller for 2-dimensional linear time invariant systems with decoupled dynamics and a single control input.In chapter 7 the close-to-optimal adaptive RHOC of chapter 5 and three existing control strategies (timers, NH 4 -bounds based and ORP, Oxidation Reduction Potential, based) for N-removal in continuously mixed alternating reactors are compared by means of simulation. The simulations are carried out in SIMBA TM, a commercially available application within the MATLAB/SIMULINK TMenvironment, based on the Activated Sludge Model no. 1. Drawback of simulations is that the dynamics of both the sensors and the process need to be modelled. And even the best model of the ASP is nothing but a poor resemblance of the real process. However, a fair experimental comparison of multiple controllers is impossible, not only for financial reasons. Simultaneous experimental testing would require the availability of multiple identical plants in parallel. Sequential testing on one plant would disrupt the results by changes in process conditions and influent, disabling a mutual comparison. Hence simulation is the best way to compare different control-schemes. It appears that three totally different controllers (timers, NH 4 -bounds based and adaptive RHOC) can achieve a more or less equal performance, if tuned optimally. Adaptive RHOC turns out to be superior in terms of sensitivity to suboptimal tunings. The timers approach is attractive for its simplicity, but very sensitive to suboptimal tuning.Chapter 8 describes a simulation study with the scope to optimise the plant design and operation strategy of alternating activated sludge processes for N-removal with two hydraulically connected reactors. The methodology is to simulate the application of RHOC to a range of different plant designs within this class of systems. The RHOC algorithm is obtained by reformulating the controller of chapter 4 for a 2-reactors system. It appears that in the optimal process design the two reactors are placed in series, the first reactor is about four times as large as the second one. A conceptually simple feedback controller straightforwardly implements the improved operation strategy. The results of this chapter strongly advocate the simulation of optimal control applied to complex process models. The results are unexpected and indicate a significant outperformance of the current operation strategy. This kind of simulation studies at least serves as an ideas generator.Chapter 9 presents a grey-box modelling approach for the identification of the nonlinear DO dynamics. Herein, singular value decomposition of the locally available Jacobian matrix, or equivalently eigenvalue decomposition of the parameter covariance matrix, as well as parameter transformation are essential techniques. The use of respiration rate measurements greatly simplifies the modelling procedure. The approach is amongst others capable of identifying the non-linear function k L a ( q air ), i.e. the relationship between k L a and the aeration input signal q air . This is especially valuable in experimental identification of the relationship between k L a ( q air ) and the design of (newly developed) aeration equipment, the use of specific carrier materials in aerated reactors, or the presence of certain detergents. After all a higher k L a at a given q air results in a higher efficiency of energy usage for aeration, and hence identification of k L a ( q air ) for newly developed equipment can yield important sales arguments.Chapters 10 and 11 both deal with excitation of the respiration chamber dynamics in a continuous flow respirometer with the objective to extract additional information from its dissolved oxygen (DO) sensor readings. Chapter 10 is an effort to improve the accuracy of the BOD st -estimation technique developed by Spanjers et al . (1994). Contrary to expectation, the estimates still suffer from unacceptable inaccuracy due to large parameter correlation. However, a slight modification in the measurement strategy is proposed which is expected to enable more accurate estimation. The results of experiments with this modified measurement strategy are reported in chapter 11. The estimation results convincingly discourage further efforts to identify sludge kinetics and BOD st from this type of experiments.The two other objectives of chapter 11 are the identification of the DO-sensor dynamics and the dilution rate in a continuous flow respirometer by excitation of the respiration chamber dynamics. Two separate simple procedures are presented. Both procedures consist of on-purpose in-sensor experiments succeeded by an ordinary least squares estimation step. The feasibility of both procedures is verified in experiments with activated sludge, fed with municipal wastewater. Large experimental data sets are presented, which strongly advocate the on-line incorporation of both procedures in the everyday operation of the respirometer.In chapter 12 those conclusions drawn in the individual chapters which are of direct relevance to practitioners are summarized. Moreover some remaining ideas, which I believe are novel and likely to be succesfull, are shortly expounded in chapter 12 as well. The ideas concern: 1) Meeting N-total effluent standards by permitting elevated effluent NH 4 ; 2) Control explicitly aiming at meeting yearly averaged effluent standards; 3) The use of pH-measurements for continuous on-line tuning of timers in a timer-based operation strategy for alternating N-removal in a continuously mixed ASP reactor.</p

Instrumentation and control of anaerobic digestion processes: a review and some research challenges

The final publication is available at Springer via http://dx.doi.org/10.1007/s11157-015-9382-6[EN] To enhance energy production from methane or resource recovery from digestate, anaerobic digestion processes require advanced instrumentation and control tools. Over the years, research on these topics has evolved and followed the main fields of application of anaerobic digestion processes: from municipal sewage sludge to liquid mainly industrial then municipal organic fraction of solid waste and agricultural residues. Time constants of the processes have also changed with respect to the treated waste from minutes or hours to weeks or months. Since fast closed loop control is needed for short time constant processes, human operator is now included in the loop when taking decisions to optimize anaerobic digestion plants dealing with complex solid waste over a long retention time. Control objectives have also moved from the regulation of key variables measured online to the prediction of overall process perfor- mance based on global off-line measurements to optimize the feeding of the processes. Additionally, the need for more accurate prediction of methane production and organic matter biodegradation has impacted the complexity of instrumentation and should include a more detailed characterization of the waste (e.g., biochemical fractions like proteins, lipids and carbohydrates)andtheirbioaccessibility andbiodegradability characteristics. However, even if in the literature several methodologies have been developed to determine biodegradability based on organic matter characterization, only a few papers deal with bioaccessibility assessment. In this review, we emphasize the high potential of some promising techniques, such as spectral analysis, and we discuss issues that could appear in the near future concerning control of AD processes.The authors acknowledge the financial support of INRA (the French National Institute for Agricultural Research), the French National Research Agency (ANR) for the "Phycover" project (project ANR-14-CE04-0011) and ADEME for Inter-laboratory assay financial support.Jimenez, J.; Latrille, E.; Harmand, J.; Robles Martínez, Á.; Ferrer Polo, J.; Gaida, D.; Wolf, C.... (2015). Instrumentation and control of anaerobic digestion processes: a review and some research challenges. Reviews in Environmental Science and Biotechnology. 14(4):615-648. doi:10.1007/s11157-015-9382-6S615648144Aceves-Lara CA, Latrille E, Steyer JP (2010) Optimal control of hydrogen production in a continuous anaerobic fermentation bioreactor. 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Realising full-scale control in wastewater treatment systems using in situ nutrient sensors

Abstract A major change in paradigm is taking place in the operation of wastewater treatment plants as automatic process control is becoming feasible. This change is due to a number of different reasons, not least the development of online nutrient sensors, which measure the key parameters in the biological nutrient removal processes, i.e. ammonium, nitrate and phosphate. The thesis is about realising full-scale control in wastewater treatment systems using in situ nutrient sensors. The main conclusion of the work is that it is possible to significantly improve the operational performance in full-scale plants by means of relatively simple control structures and controllers based on in situ nutrient sensors. The in situ location should be emphasised as this results in short dead time, hence making simple feedback loops based on proportional and integral actions effective means to control the processes. This conclusion has been reached based on full-scale experiments, where various controllers and control structures for the biological removal of nitrogen and the chemical removal of phosphorous have been tested. The full-scale experiments have shown that it is possible to provide significant savings in energy consumption and precipitation chemicals consumption, reduction in sludge production and improvement of the effluent water quality. The conclusions are supported by model simulations using the COST benchmark simulation platform. The simulations are used for investigating issues regarding the interactions between the main control handles working in the medium time frame (relative gain array analysis). The simulations have also been used for testing various control structures and controllers. Controllers for the following types of control are suggested and tested: „h Control of aeration to obtain a certain effluent ammonium concentration; „h Control of internal recirculation flow rate to obtain maximum inorganic nitrogen removal; „h Control of external carbon dosage together with internal recirculation flow rate to obtain a certain effluent total inorganic nitrogen concentration; „h Optimisation of the choice of sludge age. Additionally, a procedure for implementing new control structures based on nutrient sensor has been proposed. The procedure involves an initial analysis phase, a monitoring phase, an experimenting phase and an automatic process control phase. An international survey with the aim to investigate the correspondence between ICA (instrumentation, control and automation) utilisation and plant performance has been carried out. The survey also gives insight into the current state of ICA applications at wastewater treatment plants