1,670 research outputs found

    Cell-scale hemolysis evaluation of intervenient ventricular assist device based on dissipative particle dynamics

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    Most of the existing hemolysis mechanism studies are carried out on the macro flow scale. They assume that the erythrocyte membranes with different loads will suffer the same damage, which obviously has limitations. Thus, exploring the hemolysis mechanism through the macroscopic flow field information is a tough challenge. In order to further understand the non-physiological shear hemolysis phenomenon at the cell scale, this study used the coarse-grained erythrocytes damage model at the mesoscopic scale based on the transport dissipative particle dynamics (tDPD) method. Combined with computational fluid dynamics the hemolysis of scalarized shear stress (τ) in the clearance of “Impella 5.0” was evaluated under the Lagrange perspective and Euler perspective. The results from the Lagrange perspective showed that the change rate of scaled shear stress (τ˙) was the most critical factor in damaging RBCs in the rotor region of “Impella 5.0”and other transvalvular micro-axial blood pumps. Then, we propose a dimensionless number Dk with time integration based on τ˙ to evaluate hemolysis. The Dissipative particle dynamics simulation results are consistent with the Dk evaluation results, so τ˙ may be an important factor in the hemolysis of VADs. Finally, we tested the hemolysis of 30% hematocrit whole blood in the “Impella 5.0” shroud clearance from the Euler perspective. Relevant results indicate that because of the wall effect, the RBCs near the impeller side are more prone to damage, and most of the cytoplasm is also gathered at the rotor side

    Multi-Scale Fluctuations in Non-Equilibrium Systems: Statistical Physics and Biological Application

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    Understanding how fluctuations continuously propagate across spatial scales is fundamental for our understanding of inanimate matter. This is exemplified by self-similar fluctuations in critical phenomena and the propagation of energy fluctuations described by the Kolmogorov-Law in turbulence. Our understanding is based on powerful theoretical frameworks that integrate fluctuations on intermediary scales, as in renormalisation group or coupled mode theory. In striking contrast to typical inanimate systems, living matter is typically organised into a hierarchy of processes on a discrete set of spatial scales: from biochemical processes embedded in dynamic subcellular compartments to cells giving rise to tissues. Therefore, the understanding of living matter requires novel theories that predict the interplay of fluctuations on multiple scales of biological organisation and the ensuing emergent degrees of freedom. In this thesis, we derive a general theory of the multi-scale propagation of fluctuations in non-equilibrium systems and show that such processes underlie the regulation of cellular behaviour. Specifically, we draw on paradigmatic systems comprising stochastic many-particle systems undergoing dynamic compartmentalisation. We first derive a theory for emergent degrees of freedom in open systems, where the total mass is not conserved. We show that the compartment dynamics give rise to the localisation of probability densities in phase space resembling quasi-particle behaviour. This emergent quasi-particle exhibits fundamentally different response kinetics and steady states compared to systems lacking compartment dynamics. In order to investigate a potential biological function of such quasi-particle dynamics, we then apply this theory to the regulation of cell death. We derive a model describing the subcellular processes that regulate cell death and show that the quasi-particle dynamics gives rise to a kinetic low-pass filter which suppresses the response of the cell to fast fluituations in cellular stress signals. We test our predictions experimentally by quantifying cell death in cell cultures subject to stress stimuli varying in strength and duration. In closed systems, where the total mass is conserved, the effect of dynamic compartmentalisation depends on details of the kinetics on the scale of the stochastic many-particle dynamics. Using a second quantisation approach, we derive a commutator relation between the kinetic operators and the change in total entropy. Drawing on this, we show that the compartment dynamics alters the total entropy if the kinetics of the stochastic many-particle dynamics violate detailed balance. We apply this mechanism to the activation of cellular immune responses to RNA-virus infections. We show that dynamic compartmentalisation in closed systems gives rise to giant density fluctuations. This facilitates the emergence of gelation under conditions that violate theoretical gelation criteria in the absence of compartment dynamics. We show that such multi-scale gelation of protein complexes on the membranes of dynamic mitochondria governs the innate immune response. Taken together, we provide a general theory describing the multi-scale propagation of fluctuations in biological systems. Our work pioneers the development of a statistical physics of such systems and highlights emergent degrees of freedom spanning different scales of biological organisation. By demonstrating that cells manipulate how fluctuations propagate across these scales, our work motivates a rethinking of how the behaviour of cells is regulated

    Microfluidic systems based on electroactive polymers technology

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    Dielectric elastomer actuators (DEAs) have been widely investigated for more than 30 years. Lately, several fabrication methods have successfully allowed the creation of very thin elastomer and electrode layers. The development of attractive applications, in which DEAs offer advantages over conventional technologies, is thus necessary for the advance of the technology. In this work, new biocompatible microfluidic devices based on DEAs are developed. In the first part of this thesis, several prototypes of peristaltic pumps of single layer dielectric elastomer actuators are designed, manufactured and characterized. Although these prototypes were not able to produce fluid flow, novel insights into the capabilities of Electroactive Polymer technology were gained. In the second part of this work, a pumping micromixer as a novel application of dielectric elastomer stacked actuators is manufactured. The pumping micromixer is based on peristaltic movements, which gently act as a mixer and a pump for microfluidics. Experimental data show a maximal flow rate of 21.5 µL/min at 10 Hz. Image analysis at the outlet proves a 50/50 mixing when all actuators are functioning at the same pace and voltage. The performance of the pumping micromixer is further studied with the Finite Element Method, using the COMSOL Multiphysics® software. Simulations demonstrate the versatility of the pumping characteristics of such a microdevice, from very few µL/min to mL/min, and from a very low pressure in the range of Pa to hundreds of kPa, by only changing the duty cycle, phase shift and actuation frequency

    Advanced Manufacturing of Multilayer Ceramic Composites for Application in Solid Oxide Fuel Cells

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    This thesis investigates advanced techniques to control multilayer ceramic composite (MCC) 3D geometry and layer architecture. MCCs have tremendous potential to significantly change a variety of fields due to their ability to withstand extreme environments. However, our limited ability to shape them into complex objects impedes these efforts. To address this issue, two techniques have been introduced: fill coating and bilayer shrinkage driven self-shaping. Central to both techniques is the control of residual stresses experienced by MCCs during sintering. In the case of fill coating and the control of layer architecture, these residual stresses needed to be reduced to prevent the fracture of the novel internal cathode tubular solid oxide fuel cell (IC-tSOFC). This was achieved with the adoption of extended sintering procedures which promoted plastic deformation processes like creep stress relaxation. The novel fill coating technique used to produce IC-tSOFCs was then investigated using scanning electron microscopy (SEM) to ensure that the deposited films were highly uniform and comparable to films deposited using the more mature dip coating technique. The electrochemical performance of the IC-tSOFC was then thoroughly evaluated on a variety of fuel streams including pure hydrogen, dilute hydrogen, simulated exhaust from a boiler, and simulated exhaust from a two-stroke internal combustion engine. The second focus of this thesis takes advantage of the residual stresses that complicated IC-tSOFC development rather than dissipating them. By using the mismatch in the thermal expansion coefficient between adjacent layers within planar MCCs, curvature may be introduced. Substrates were produced using tape casting and a thin film was then added to this substrate using aerosol spray deposition. By controlling the thickness of the substrate and film, as well as the 2D shape of the substrate and pattern of the applied film, the curvature and shape of the final self-formed part was controlled. Beyond demonstration of this novel manufacturing technique, investigation into curvature and shape prediction using analytical and finite element method (FEM) modeling enabled the development of a methodology to design parts using self-shaping. Initial investigations focused on predicting curvature. Though a disagreement between modeling and experiment was observed, an experimental TEC was introduced to replicate experimental results in FEM modeling. This understanding of the 2D curvature was then extended to three dimensions to analyze shape. Predictions regarding bifurcation between cap-like and tube-like deformation modes was applied to the ceramic system using FEM modeling and experiment. These predictions were shown to be consistent with theoretical understanding. Similarly, bending direction for tube-like deformation was shown to be generally consistent with theoretical understanding, but here FEM modeling struggled to reliably predict the final 3D geometry of shapes with high degrees of symmetry, and experimental samples experienced misorientation of bending, indicating that models may need to be expanded to include a greater variety of forces controlling deformation. Overall, this thesis shows successful development of novel manufacturing techniques to enable wider application of ceramic materials. While the IC-tSOFC introduces new combined heat and power-SOFC systems to be explored, self-shaping ceramics introduces a variety of fundamental questions regarding the underlying mechanism driving bilayer shrinkage within MCCs as well as full understanding of the interaction between 2D substrate shape and film pattern at any scale

    Dynamically Stable Radiation Pressure Propulsion of Flexible Lightsails for Interstellar Exploration

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    Lightsail spacecraft, propelled to relativistic velocities via photon pressure using high power density laser radiation, offer a potentially new route to space exploration within and beyond the solar system, extending to interstellar distances. Such missions will require meter-scale lightsails of submicron thickness, posing substantial challenges for materials science and engineering. We analyze the structural and photonic design of flexible lightsails, developing a mesh-based multiphysics simulator based on linear elastic theory, treating the lightsail as a flexible membrane rather than a rigid body. We find that flexible lightsail membranes can be spin stabilized to prevent shape collapse during acceleration, and that certain lightsail shapes and designs offer beam-riding stability despite the deformations caused by photon pressure and thermal expansion. Excitingly, nanophotonic lightsails based on planar silicon nitride membranes patterned with suitably designed optical metagratings exhibit both mechanically and dynamically stable propulsion along the pump laser axis. These advances suggest that laser-driven acceleration of membrane-like lightsails to the relativistic speeds needed to access interstellar distances is conceptually feasible, and that fabrication of such lightsails may be within the reach of modern microfabrication technology.Comment: 14 pages, 6 figures; plus 18-page SI with figures and linked video

    Advanced Materials and Technologies in Nanogenerators

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    This reprint discusses the various applications, new materials, and evolution in the field of nanogenerators. This lays the foundation for the popularization of their broad applications in energy science, environmental protection, wearable electronics, self-powered sensors, medical science, robotics, and artificial intelligence

    Emerging topics in nanophononics and elastic, acoustic, and mechanical metamaterials: an overview

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    This broad review summarizes recent advances and “hot” research topics in nanophononics and elastic, acoustic, and mechanical metamaterials based on results presented by the authors at the EUROMECH 610 Colloquium held on April 25–27, 2022 in Benicássim, Spain. The key goal of the colloquium was to highlight important developments in these areas, particularly new results that emerged during the last two years. This work thus presents a “snapshot” of the state-of-the-art of different nanophononics- and metamaterial-related topics rather than a historical view on these subjects, in contrast to a conventional review article. The introduction of basic definitions for each topic is followed by an outline of design strategies for the media under consideration, recently developed analysis and implementation techniques, and discussions of current challenges and promising applications. This review, while not comprehensive, will be helpful especially for early-career researchers, among others, as it offers a broad view of the current state-of-the-art and highlights some unique and flourishing research in the mentioned fields, providing insight into multiple exciting research directions

    Designing a New Tactile Display Technology and its Disability Interactions

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    People with visual impairments have a strong desire for a refreshable tactile interface that can provide immediate access to full page of Braille and tactile graphics. Regrettably, existing devices come at a considerable expense and remain out of reach for many. The exorbitant costs associated with current tactile displays stem from their intricate design and the multitude of components needed for their construction. This underscores the pressing need for technological innovation that can enhance tactile displays, making them more accessible and available to individuals with visual impairments. This research thesis delves into the development of a novel tactile display technology known as Tacilia. This technology's necessity and prerequisites are informed by in-depth qualitative engagements with students who have visual impairments, alongside a systematic analysis of the prevailing architectures underpinning existing tactile display technologies. The evolution of Tacilia unfolds through iterative processes encompassing conceptualisation, prototyping, and evaluation. With Tacilia, three distinct products and interactive experiences are explored, empowering individuals to manually draw tactile graphics, generate digitally designed media through printing, and display these creations on a dynamic pin array display. This innovation underscores Tacilia's capability to streamline the creation of refreshable tactile displays, rendering them more fitting, usable, and economically viable for people with visual impairments
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