Wing and body motion during flight initiation in Drosophila revealed by automated visual tracking

The fruit fly Drosophila melanogaster is a widely used model organism in studies of genetics, developmental biology and biomechanics. One limitation for exploiting Drosophila as a model system for behavioral neurobiology is that measuring body kinematics during behavior is labor intensive and subjective. In order to quantify flight kinematics during different types of maneuvers, we have developed a visual tracking system that estimates the posture of the fly from multiple calibrated cameras. An accurate geometric fly model is designed using unit quaternions to capture complex body and wing rotations, which are automatically fitted to the images in each time frame. Our approach works across a range of flight behaviors, while also being robust to common environmental clutter. The tracking system is used in this paper to compare wing and body motion during both voluntary and escape take-offs. Using our automated algorithms, we are able to measure stroke amplitude, geometric angle of attack and other parameters important to a mechanistic understanding of flapping flight. When compared with manual tracking methods, the algorithm estimates body position within 4.4±1.3% of the body length, while body orientation is measured within 6.5±1.9 deg. (roll), 3.2±1.3 deg. (pitch) and 3.4±1.6 deg. (yaw) on average across six videos. Similarly, stroke amplitude and deviation are estimated within 3.3 deg. and 2.1 deg., while angle of attack is typically measured within 8.8 deg. comparing against a human digitizer. Using our automated tracker, we analyzed a total of eight voluntary and two escape take-offs. These sequences show that Drosophila melanogaster do not utilize clap and fling during take-off and are able to modify their wing kinematics from one wingstroke to the next. Our approach should enable biomechanists and ethologists to process much larger datasets than possible at present and, therefore, accelerate insight into the mechanisms of free-flight maneuvers of flying insects

Combination of Annealing Particle Filter and Belief Propagation for 3D Upper Body Tracking

3D upper body pose estimation is a topic greatly studied by the computer vision society because it is useful in a great number of applications, mainly for human robots interactions including communications with companion robots. However there is a challenging problem: the complexity of classical algorithms that increases exponentially with the dimension of the vectors’ state becomes too difficult to handle. To tackle this problem, we propose a new approach that combines several annealing particle filters defined independently for each limb and belief propagation method to add geometrical constraints between individual filters. Experimental results on a real human gestures sequence will show that this combined approach leads to reliable results

Functional Rotation Axis Based Approach for Estimating Hip Joint Angles Using Wearable Inertial Sensors: Comparison to Existing Methods

Wearable sensors are at the heart of the digital health revolution. Integral to the use of these sensors for monitoring conditions impacting balance and mobility are accurate estimates of joint angles. To this end a simple and novel method of estimating hip joint angles from small wearable magnetic and inertial sensors is proposed and its performance is established relative to optical motion capture in a sample of human subjects. Improving upon previous work, this approach does not require precise sensor placement or specific calibration motions, thereby easing deployment outside of the research laboratory. Specific innovations include the determination of sensor to segment rotations based on functionally determined joint centers, and the development of a novel filtering algorithm for estimating the relative orientation of adjacent body segments. Hip joint angles and range of motion determined from the proposed approach and an existing method are compared to those from an optical motion capture system during walking at a variety of speeds and tasks designed to exercise the hip through its full range of motion. Results show that the proposed approach estimates flexion/extension angle more accurately (RMSE from 7.08 to 7.29 deg) than the existing method (RMSE from 11.64 deg to 14.33 deg), with similar performance for the other anatomical axes. Agreement of each method with optical motion capture is further characterized by considering correlation and regression analyses. Mean ranges of motion for the proposed method are not largely different from those reported by motion capture, and showed similar values to the existing method. Results indicate that this algorithm provides a promising approach for estimating hip joint angles using wearable inertial sensors, and would allow for use outside of constrained research laboratories

International Master Program on Mechatronic Systems for Rehabilitation

This paper describes a second level of university course called Master in France, Laurea Magistrale in Italy, jointly organized by UPMC Université Pierre et Marie Curie – Paris (France) and UniBS Università degli Studi di Brescia (Italy). During this "double-degree" course, students coming from these universities share two semesters while studying subjects related to mechatronics and biomechanics. At the end of the program the students obtain a double degree from both universities

Constructing a reference standard for sports science and clinical movement sets using IMU-based motion capture technology

Motion analysis has improved greatly over the years through the development of low-cost inertia sensors. Such sensors have shown promising accuracy for both sport and medical applications, facilitating the possibility of a new reference standard to be constructed. Current gold standards within motion capture, such as high-speed camera-based systems and image processing, are not suitable for many movement-sets within both sports science and clinical movement analysis due to restrictions introduced by the movement sets. These restrictions include cost, portability, local environment constraints (such as light level) and poor line of sight accessibility. This thesis focusses on developing a magnetometer-less IMU-based motion capturing system to detect and classify two challenging movement sets: Basic stances during a Shaolin Kung Fu dynamic form, and severity levels from the modified UPDRS (Unified Parkinson’s Disease Rating Scale) analysis tapping exercise. This project has contributed three datasets. The Shaolin Kung Fu dataset is comprised of 5 dynamic movements repeated over 350 times by 8 experienced practitioners. The dataset was labelled by a professional Shaolin Kung Fu master. Two modified UPDRS datasets were constructed, one for each of the two locations measured. The modified UPDRS datasets comprised of 5 severity levels each with 100 self-emulated movement samples. The modified UPDRS dataset was labelled by a researcher in neuropsychological assessment. The errors associated with IMU systems has been reduced significantly through a combination of a Complementary filter and applying the constraints imposed by the range of movements available in human joints. Novel features have been extracted from each dataset. A piecewise feature set based on a moving window approach has been applied to the Shaolin Kung Fu dataset. While a combination of standard statistical features and a Durbin Watson analysis has been extracted from the modified UPDRS measurements. The project has also contributed a comparison of 24 models has been done on all 3 datasets and the optimal model for each dataset has been determined. The resulting models were commensurate with current gold standards. The Shaolin Kung Fu dataset was classified with the computational costly fine decision tree algorithm using 400 splits, resulting in: an accuracy of 98.9%, a precision of 96.9%, a recall value of 99.1%, and a F1-score of 98.0%. A novel approach of using sequential forward feature analysis was used to determine the minimum number of IMU devices required as well as the optimal number of IMU devices. The modified UPDRS datasets were then classified using a support vector machine algorithm requiring various kernels to achieve their highest accuracies. The measurements were repeated with a sensor located on the wrist and finger, with the wrist requiring a linear kernel and the finger a quadratic kernel. Both locations achieved an accuracy, precision, recall, and F1-score of 99.2%. Additionally, the project contributed an evaluation to the effect sensor location has on the proposed models. It was concluded that the IMU-based system has the potential to construct a reference standard both in sports science and clinical movement analysis. Data protection security and communication speeds were limitations in the system constructed due to the measured data being transferred from the devices via Bluetooth Low Energy communication. These limitations were considered and evaluated in the future works of this project