The Arabidopsis NADPH oxidases RbohD and RbohF display differential expression patterns and contributions during plant immunity

Plant NADPH oxidases, also known as respiratory burst oxidase homologues (RBOHs), produce reactive oxygen species (ROS) that perform a wide range of functions. RbohD and RbohF, two of the 10 Rboh genes present in Arabidopsis, are pleiotropic and mediate diverse physiological processes including the response to pathogens. We hypothesized that the spatio-temporal control of RbohD and RbohF gene expression might be critical in determining their multiplicity of functions. Transgenic Arabidopsis plants with RbohD and RbohF promoter fusions to β-glucuronidase and Luciferase reporter genes were generated. Analysis of these plants revealed a differential expression pattern for RbohD and RbohF throughout plant development and during immune responses. RbohD and RbohF gene expression was differentially modulated by pathogen-associated molecular patterns. Histochemical stains and in vivo expression analysis showed a correlation between the level of RbohD and RbohF promoter activity, H2O2 accumulation and the amount of cell death in response to the pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 and the necrotrophic fungus Plectosphaerella cucumerina. A promoter-swap strategy revealed that the promoter region of RbohD was required to drive production of ROS by this gene in response to pathogens. Moreover, RbohD promoter was activated during Arabidopsis interaction with a non-virulent P. cucumerina isolate, and susceptibility tests with the double mutant rbohD rbohF uncovered a new function for these oxidases in basal resistance. Altogether, our results suggest that differential spatio-temporal expression of the Rboh genes contributes to fine-tune RBOH/NADPH oxidase-dependent ROS production and signaling in Arabidopsis immunity

A coordination protocol for user-customisable cloud policy monitoring

Cloud computing will see a increasing demand for end-user customisation and personalisation of multi-tenant cloud service offerings. Combined with an identified need to address QoS and governance aspects in cloud computing, a need to provide user-customised QoS and governance policy management and monitoring as part of an SLA management infrastructure for clouds arises. We propose a user-customisable policy definition solution that can be enforced in multi-tenant cloud offerings through an automated instrumentation and monitoring technique. We in particular allow service processes that are run by cloud and SaaS providers to be made policy-aware in a transparent way

A situational approach for the definition and tailoring of a data-driven software evolution method

Successful software evolution heavily depends on the selection of the right features to be included in the next release. Such selection is difficult, and companies often report bad experiences about user acceptance. To overcome this challenge, there is an increasing number of approaches that propose intensive use of data to drive evolution. This trend has motivated the SUPERSEDE method, which proposes the collection and analysis of user feedback and monitoring data as the baseline to elicit and prioritize requirements, which are then used to plan the next release. However, every company may be interested in tailoring this method depending on factors like project size, scope, etc. In order to provide a systematic approach, we propose the use of Situational Method Engineering to describe SUPERSEDE and guide its tailoring to a particular context.Peer ReviewedPostprint (author's final draft

Formal certification and compliance for run-time service environments

With the increased awareness of security and safety of services in on-demand distributed service provisioning (such as the recent adoption of Cloud infrastructures), certification and compliance checking of services is becoming a key element for service engineering. Existing certification techniques tend to support mainly design-time checking of service properties and tend not to support the run-time monitoring and progressive certification in the service execution environment. In this paper we discuss an approach which provides both design-time and runtime behavioural compliance checking for a services architecture, through enabling a progressive event-driven model-checking technique. Providing an integrated approach to certification and compliance is a challenge however using analysis and monitoring techniques we present such an approach for on-going compliance checking

Evolving IT management frameworks towards a sustainable future

Information Technology (IT) Management Frameworks are a fundamental tool used by IT professionals to efficiently manage IT resources and are globally applied to IT service delivery and management. Sustainability is a recent notion that describes the need for economic, environmental and social development with- out compromising the ability of future generations to meet their own needs; this applies to businesses as well as society in general. Unfortunately, IT Management Frameworks do not take sustainability into account. To the practitioner this paper demonstrates sustainability integration thereby allowing CIOs and IT managers to improve the sustainability of their organisation. To the researcher this paper argues that sustainability concerns need to be provided to IT Management through its integration into the mainstream of IT Management Frameworks. This is demonstrated through the high-level integration of sustainability in Six Sigma, C OBI T, ITIL and PRINCE2