Impact of Diet and Quality Grade on Shelf Life of Beef Steaks

Steers were fed a diet containing dry rolled corn, steam flaked corn, dry rolled corn with 30% dried distillers grains, or steam flaked corn with 30% dried distillers grains. Strip loins from upper 2/3 Choice and Select- grade carcasses were obtained to evaluate the effects of diet and quality grade on shelf life characteristics. Strip loins were aged for 2, 9, 16, or 23 days. Results suggest that steaks from cattle fed steam flaked corn (with or without dried distillers grains) and from cattle fed dried distillers grains (regardless of corn type) had higher levels of many unsaturated fatty acids, more discoloration, and greater lipid oxidation compared to the dry rolled corn treatments or the no dried distillers grains treatments, respectively. Feeding of dry rolled corn or diets without dried distillers grains maintained red color better during retail display. Choice- grade steaks had significantly higher levels of unsaturated fatty acids like 18:2 and total polyunsaturated fatty acids than Select- grade steaks but did not diff er in color stability or oxidation. These data indicate the longest shelf life will occur when cattle are fed diets containing dry rolled corn (versus steam flaked corn) or without dried distillers grains (versus with dried distillers grains) and that both steam flaked corn and distillers grains have a negative impact on shelf life. Quality grade did not affect color stability

Genetic contribution to radiographic severity in osteoarthritis of the knee

Objective Knee osteoarthritis (OA) has a significant genetic component. The authors have assessed the role of three variants reported to influence risk of knee OA with p<5×10–8 in determining patellofemoral and tibiofemoral Kellgren Lawrence (K/L) grade in knee OA cases. Methods 3474 knee OA cases with sky-line and weight-bearing antero-posterior x-rays of the knee were selected based on the presentation of K/L grade ≥2 at either the tibiofemoral or patellofemoral compartments for one or both knees. Patients belonging to three UK cohorts, were genotyped for rs143383, rs4730250 and rs11842874 mapping to the GDF5, COG5 and MCF2L genes, respectively. The association between tibiofemoral K/L grade and patellofemoral K/L grade was assessed after adjusting for age, gender and body mass index. Results No significant association was found between the rs4730250 and radiographic severity. The rs11842874 mapping to MCF2L was found to be nominally significantly associated with patellofemoral K/L grade as a quantitative trait (p=0.027) but not as a binary trait. The GDF5 single nucleotide polymorphism rs143383 was associated with tibiofemoral K/L grade (β=0.05 (95% CI 0.02 to 0.08) p=0.0011). Conclusions Our data indicate that within individuals affected by radiographic knee OA, OAGDF5 has a modest but significant effect on radiographic severity after adjustment for the major risk factors

To grade or not to grade the application of safety requirements for transvenous lead extraction: Experience with 2216 procedures

Background: Transvenous lead extraction (TLE) procedures are now increasingly safe, but there is still a risk of major complications (MC). Aims: Assessment of the impact of the organization of TLE on the safety of procedures. Methods: We analyzed 2216 TLE preformed in two centers in years 2006‒2021 and compared three organizational models of  procedure: (1) TLE in electrophysiology laboratory (EP-LAB) with intravenous analgesia/sedation;  (2) TLE with the grading of safety requirements (high-risk patients in the cardiac surgery operating theatre, the remained in EP-LAB); (3) TLE in the hybrid room in all patients under general anaesthesia with transoesophageal echocardiographic (TEE) monitoring. The safety of procedures and mortality after TLE in three-year follow-up were assessed. Results: The rate of MC in EP-lab was 1.55% and the rate of procedure-related deaths (PRD) was 0.33%. Using the graded approach to safety requirements, the percentage of MC was 2.61% and PRD 0.29%. When performing TLE procedures in the hybrid room, the MC percentage was 1.33% and PRD 0.00%. Long-term survival after TLE was comparable in all study groups. Conclusions: A key factor in preventing TLE-related deaths is an organization of procedure that enables emergency cardiac surgery. TLE performed in a hybrid room with cardiac surgeon in collaboration and vital signs monitoring appears to be the safest possible option for the patient.  A graded safety approach is associated with the risk of unexpected MC and PRD. Any newly established TLE center can achieve satisfactory results if optimal organizational model of the procedure is used

Hepatic resection for metastatic colorectal adenocarcinoma: A proposal of a prognostic scoring system

Background: Hepatic resection for metastatic colorectal cancer provides excellent longterm results in a substantial proportion of patients. Although various prognostic risk factors have been identified, there has been no dependable staging or prognostic scoring system for metastatic hepatic tumors. Study Design: Various clinical and pathologic risk factors were examined in 305 consecutive patients who underwent primary hepatic resections for metastatic colorectal cancer. Survival rates were estimated by the Cox proportional hazards model using the equation: S(t) = [S(o)(t)](exp(R - R(o))), where S(o)(t) is the survival rate of patients with none of the identified risk factors and R(o) = 0. Results: Preliminary multivariate analysis revealed that independently significant negative prognosticators were: (1) positive surgical margins, (2) extrahepatic tumor involvement including the lymph node(s), (3) tumor number of three or more, (4) bilobar tumors, and (5) time from treatment of the primary tumor to hepatic recurrence of 30 months or less. Because the survival rates of the 62 patients with positive margins or extrahepatic tumor were uniformly very poor, multivariate analysis was repeated in the remaining 243 patients who did not have these lethal risk factors. The reanalysis revealed that independently significant poor prognosticators were: (1) tumor number of three or more, (2) tumor size greater than 8 cm, (3) time to hepatic recurrence of 30 months or less, and (4) bilobar tumors. Risk scores (R) for tumor recurrence of the culled cohort (n = 243) were calculated by summation of coefficients from the multivariate analysis and were divided into five groups: grade 1, no risk factors (R = 0); grade 2, one risk factor (R = 0.3 to 0.7); grade 3, two risk factors (R = 0.7 to 1.1); grade 4, three risk factors (R = 1.2 to 1.6); and grade 5, four risk factors (R > 1.6). Grade 6 consisted of the 62 culled patients with positive margins or extrahepatic tumor. Kaplan-Meier and Cox proportional hazards estimated 5-year survival rates of grade 1 to 6 patients were 48.3% and 48.3%, 36.6% and 33.7%, 19.9% and 17.9%, 11.9% and 6.4%, 0% and 1.1%, and 0% and 0%, respectively (p < 0.0001). Conclusions: The proposed risk-score grading predicted the survival differences extremely well. Estimated survival as determined by the Cox proportional hazards model was similar to that determined by the Kaplan-Meier method. Verification and further improvements of the proposed system are awaited by other centers or international collaborative studies