6,515 research outputs found

    These are not the k-mers you are looking for: efficient online k-mer counting using a probabilistic data structure

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    K-mer abundance analysis is widely used for many purposes in nucleotide sequence analysis, including data preprocessing for de novo assembly, repeat detection, and sequencing coverage estimation. We present the khmer software package for fast and memory efficient online counting of k-mers in sequencing data sets. Unlike previous methods based on data structures such as hash tables, suffix arrays, and trie structures, khmer relies entirely on a simple probabilistic data structure, a Count-Min Sketch. The Count-Min Sketch permits online updating and retrieval of k-mer counts in memory which is necessary to support online k-mer analysis algorithms. On sparse data sets this data structure is considerably more memory efficient than any exact data structure. In exchange, the use of a Count-Min Sketch introduces a systematic overcount for k-mers; moreover, only the counts, and not the k-mers, are stored. Here we analyze the speed, the memory usage, and the miscount rate of khmer for generating k-mer frequency distributions and retrieving k-mer counts for individual k-mers. We also compare the performance of khmer to several other k-mer counting packages, including Tallymer, Jellyfish, BFCounter, DSK, KMC, Turtle and KAnalyze. Finally, we examine the effectiveness of profiling sequencing error, k-mer abundance trimming, and digital normalization of reads in the context of high khmer false positive rates. khmer is implemented in C++ wrapped in a Python interface, offers a tested and robust API, and is freely available under the BSD license at github.com/ged-lab/khmer

    Faster tuple lattice sieving using spherical locality-sensitive filters

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    To overcome the large memory requirement of classical lattice sieving algorithms for solving hard lattice problems, Bai-Laarhoven-Stehl\'{e} [ANTS 2016] studied tuple lattice sieving, where tuples instead of pairs of lattice vectors are combined to form shorter vectors. Herold-Kirshanova [PKC 2017] recently improved upon their results for arbitrary tuple sizes, for example showing that a triple sieve can solve the shortest vector problem (SVP) in dimension dd in time 20.3717d+o(d)2^{0.3717d + o(d)}, using a technique similar to locality-sensitive hashing for finding nearest neighbors. In this work, we generalize the spherical locality-sensitive filters of Becker-Ducas-Gama-Laarhoven [SODA 2016] to obtain space-time tradeoffs for near neighbor searching on dense data sets, and we apply these techniques to tuple lattice sieving to obtain even better time complexities. For instance, our triple sieve heuristically solves SVP in time 20.3588d+o(d)2^{0.3588d + o(d)}. For practical sieves based on Micciancio-Voulgaris' GaussSieve [SODA 2010], this shows that a triple sieve uses less space and less time than the current best near-linear space double sieve.Comment: 12 pages + references, 2 figures. Subsumed/merged into Cryptology ePrint Archive 2017/228, available at https://ia.cr/2017/122

    Asymptotic Analysis of Plausible Tree Hash Modes for SHA-3

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    Discussions about the choice of a tree hash mode of operation for a standardization have recently been undertaken. It appears that a single tree mode cannot address adequately all possible uses and specifications of a system. In this paper, we review the tree modes which have been proposed, we discuss their problems and propose remedies. We make the reasonable assumption that communicating systems have different specifications and that software applications are of different types (securing stored content or live-streamed content). Finally, we propose new modes of operation that address the resource usage problem for the three most representative categories of devices and we analyse their asymptotic behavior

    Practical Evaluation of Lempel-Ziv-78 and Lempel-Ziv-Welch Tries

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    We present the first thorough practical study of the Lempel-Ziv-78 and the Lempel-Ziv-Welch computation based on trie data structures. With a careful selection of trie representations we can beat well-tuned popular trie data structures like Judy, m-Bonsai or Cedar

    Lessons learned from the design of a mobile multimedia system in the Moby Dick project

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    Recent advances in wireless networking technology and the exponential development of semiconductor technology have engendered a new paradigm of computing, called personal mobile computing or ubiquitous computing. This offers a vision of the future with a much richer and more exciting set of architecture research challenges than extrapolations of the current desktop architectures. In particular, these devices will have limited battery resources, will handle diverse data types, and will operate in environments that are insecure, dynamic and which vary significantly in time and location. The research performed in the MOBY DICK project is about designing such a mobile multimedia system. This paper discusses the approach made in the MOBY DICK project to solve some of these problems, discusses its contributions, and accesses what was learned from the project

    Evaluation of network coding techniques for a sniper detection application

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    This paper experimentally studies the reliability and delay of flooding based multicast protocols for a sniper detection application. In particular using an emulator it studies under which conditions protocols based on network coding deliver performance improvements compared to classic flooding. It then presents an implementation of such protocols on mobile phones
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