Complexity of Verification in Self-Assembly with Prebuilt Assemblies

We analyze the complexity of two fundamental verification problems within a generalization of the two-handed tile self-assembly model (2HAM) where initial system assemblies are not restricted to be singleton tiles, but may be larger pre-built assemblies. Within this model we consider the producibility problem, which asks if a given tile system builds, or produces, a given assembly, and the unique assembly verification (UAV) problem, which asks if a given system uniquely produces a given assembly. We show that producibility is NP-complete and UAV is coNP^{NP}-complete even when the initial assembly size and temperature threshold are both bounded by a constant. This is in stark contrast to results in the standard model with singleton input tiles where producibility is in P and UAV is in coNP for ?(1) bounded temperature and coNP-complete when temperature is part of the input. We further provide preliminary results for producibility and UAV in the case of 1-dimensional linear assemblies with pre-built assemblies, and provide polynomial time solutions

Optimal staged self-assembly of linear assemblies

We analyze the complexity of building linear assemblies, sets of linear assemblies, and O(1)-scale general shapes in the staged tile assembly model. For systems with at most b bins and t tile types, we prove that the minimum number of stages to uniquely assemble a 1 n line is (logt n + logb n t + 1). Generalizing to O(1) n lines, we prove the minimum number of stages is O( log n tb t log t b2 + log log b log t ) and ( log n tb t log t b2 ). Next, we consider assembling sets of lines and general shapes using t = O(1) tile types. We prove that the minimum number of stages needed to assemble a set of k lines of size at most O(1) n is O( k log n b2 + k p log n b + log log n) and ( k log n b2 ). In the case that b = O( p k), the minimum number of stages is (log n). The upper bound in this special case is then used to assemble \hefty shapes of at least logarithmic edge-length-to- edge-count ratio at O(1)-scale using O( p k) bins and optimal O(log n) stages

Exponential replication of patterns in the Signal Tile Assembly Model and experimental non-deterministic assembly of lines in the Probabilistic Tile Assembly Model

We introduce the problem of self-replication of rectangular two-dimensional patterns in the practically motivated Signal Tile Assembly Model (STAM), which is an extension of the aTAM. In the first part of this thesis, we construct an exponential pattern replicator that replicates a two-dimensional input pattern over some fixed alphabet of size ϕ with O(ϕ) tile types, O(ϕ) unique glues, and a signal complexity of O(1). In the second part of this thesis, we use a non-deterministic model of tile assembly to significantly reduce the tile complexity of specified-length linear assemblies, which are a particularly important substructure for building more complicated nanostructures

Two computational primitives for algorithmic self-assembly: Copying and counting

Copying and counting are useful primitive operations for computation and construction. We have made DNA crystals that copy and crystals that count as they grow. For counting, 16 oligonucleotides assemble into four DNA Wang tiles that subsequently crystallize on a polymeric nucleating scaffold strand, arranging themselves in a binary counting pattern that could serve as a template for a molecular electronic demultiplexing circuit. Although the yield of counting crystals is low, and per-tile error rates in such crystals is roughly 10%, this work demonstrates the potential of algorithmic self-assembly to create complex nanoscale patterns of technological interest. A subset of the tiles for counting form information-bearing DNA tubes that copy bit strings from layer to layer along their length

Fuel Efficient Computation in Passive Self-Assembly

In this paper we show that passive self-assembly in the context of the tile self-assembly model is capable of performing fuel efficient, universal computation. The tile self-assembly model is a premiere model of self-assembly in which particles are modeled by four-sided squares with glue types assigned to each tile edge. The assembly process is driven by positive and negative force interactions between glue types, allowing for tile assemblies floating in the plane to combine and break apart over time. We refer to this type of assembly model as passive in that the constituent parts remain unchanged throughout the assembly process regardless of their interactions. A computationally universal system is said to be fuel efficient if the number of tiles used up per computation step is bounded by a constant. Work within this model has shown how fuel guzzling tile systems can perform universal computation with only positive strength glue interactions. Recent work has introduced space-efficient, fuel-guzzling universal computation with the addition of negative glue interactions and the use of a powerful non-diagonal class of glue interactions. Other recent work has shown how to achieve fuel efficient computation within active tile self-assembly. In this paper we utilize negative interactions in the tile self-assembly model to achieve the first computationally universal passive tile self-assembly system that is both space and fuel-efficient. In addition, we achieve this result using a limited diagonal class of glue interactions

Self-Assembly of Arbitrary Shapes Using RNAse Enzymes: Meeting the Kolmogorov Bound with Small Scale Factor (extended abstract)

We consider a model of algorithmic self-assembly of geometric shapes out of square Wang tiles studied in SODA 2010, in which there are two types of tiles (e.g., constructed out of DNA and RNA material) and one operation that destroys all tiles of a particular type (e.g., an RNAse enzyme destroys all RNA tiles). We show that a single use of this destruction operation enables much more efficient construction of arbitrary shapes. In particular, an arbitrary shape can be constructed using an asymptotically optimal number of distinct tile types (related to the shape's Kolmogorov complexity), after scaling the shape by only a logarithmic factor. By contrast, without the destruction operation, the best such result has a scale factor at least linear in the size of the shape, and is connected only by a spanning tree of the scaled tiles. We also characterize a large collection of shapes that can be constructed efficiently without any scaling