947 research outputs found

    Undergraduate Catalog of Studies, 2023-2024

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    Undergraduate Catalog of Studies, 2023-2024

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    The development of liquid crystal lasers for application in fluorescence microscopy

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    Lasers can be found in many areas of optical medical imaging and their properties have enabled the rapid advancement of many imaging techniques and modalities. Their narrow linewidth, relative brightness and coherence are advantageous in obtaining high quality images of biological samples. This is particularly beneficial in fluorescence microscopy. However, commercial imaging systems depend on the combination of multiple independent laser sources or use tuneable sources, both of which are expensive and have large footprints. This thesis demonstrates the use of liquid crystal (LC) laser technology, a compact and portable alternative, as an exciting candidate to provide a tailorable light source for fluorescence microscopy. Firstly, to improve the laser performance parameters such that high power and high specification lasers could be realised; device fabrication improvements were presented. Studies exploring the effect of alignment layer rubbing depth and the device cell gap spacing on laser performance were conducted. The results were the first of their kind and produced advances in fabrication that were critical to repeatedly realising stable, single-mode LC laser outputs with sufficient power to conduct microscopy. These investigations also aided with the realisation of laser diode pumping of LC lasers. Secondly, the identification of optimum dye concentrations for single and multi-dye systems were used to optimise the LC laser mixtures for optimal performance. These investigations resulted in novel results relating to the gain media in LC laser systems. Collectively, these advancements yielded lasers of extremely low threshold, comparable to the lowest reported thresholds in the literature. A portable LC laser system was integrated into a microscope and used to perform fluorescence microscopy. Successful two-colour imaging and multi-wavelength switching ability of LC lasers were exhibited for the first time. The wavelength selectivity of LC lasers was shown to allow lower incident average powers to be used for comparable image quality. Lastly, wavelength selectivity enabled the LC laser fluorescence microscope to achieve high enough sensitivity to conduct quantitative fluorescence measurements. The development of LC lasers and their suitability to fluorescence microscopy demonstrated in this thesis is hoped to push towards the realisation of commercialisation and application for the technology

    Physical sketching tools and techniques for customized sensate surfaces

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    Sensate surfaces are a promising avenue for enhancing human interaction with digital systems due to their inherent intuitiveness and natural user interface. Recent technological advancements have enabled sensate surfaces to surpass the constraints of conventional touchscreens by integrating them into everyday objects, creating interactive interfaces that can detect various inputs such as touch, pressure, and gestures. This allows for more natural and intuitive control of digital systems. However, prototyping interactive surfaces that are customized to users' requirements using conventional techniques remains technically challenging due to limitations in accommodating complex geometric shapes and varying sizes. Furthermore, it is crucial to consider the context in which customized surfaces are utilized, as relocating them to fabrication labs may lead to the loss of their original design context. Additionally, prototyping high-resolution sensate surfaces presents challenges due to the complex signal processing requirements involved. This thesis investigates the design and fabrication of customized sensate surfaces that meet the diverse requirements of different users and contexts. The research aims to develop novel tools and techniques that overcome the technical limitations of current methods and enable the creation of sensate surfaces that enhance human interaction with digital systems.Sensorische Oberflächen sind aufgrund ihrer inhärenten Intuitivität und natürlichen Benutzeroberfläche ein vielversprechender Ansatz, um die menschliche Interaktionmit digitalen Systemen zu verbessern. Die jüngsten technologischen Fortschritte haben es ermöglicht, dass sensorische Oberflächen die Beschränkungen herkömmlicher Touchscreens überwinden, indem sie in Alltagsgegenstände integriert werden und interaktive Schnittstellen schaffen, die diverse Eingaben wie Berührung, Druck, oder Gesten erkennen können. Dies ermöglicht eine natürlichere und intuitivere Steuerung von digitalen Systemen. Das Prototyping interaktiver Oberflächen, die mit herkömmlichen Techniken an die Bedürfnisse der Nutzer angepasst werden, bleibt jedoch eine technische Herausforderung, da komplexe geometrische Formen und variierende Größen nur begrenzt berücksichtigt werden können. Darüber hinaus ist es von entscheidender Bedeutung, den Kontext, in dem diese individuell angepassten Oberflächen verwendet werden, zu berücksichtigen, da eine Verlagerung in Fabrikations-Laboratorien zum Verlust ihres ursprünglichen Designkontextes führen kann. Zudem stellt das Prototyping hochauflösender sensorischer Oberflächen aufgrund der komplexen Anforderungen an die Signalverarbeitung eine Herausforderung dar. Diese Arbeit erforscht dasDesign und die Fabrikation individuell angepasster sensorischer Oberflächen, die den diversen Anforderungen unterschiedlicher Nutzer und Kontexte gerecht werden. Die Forschung zielt darauf ab, neuartigeWerkzeuge und Techniken zu entwickeln, die die technischen Beschränkungen derzeitigerMethoden überwinden und die Erstellung von sensorischen Oberflächen ermöglichen, die die menschliche Interaktion mit digitalen Systemen verbessern

    Applying machine learning: a multi-role perspective

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    Machine (and deep) learning technologies are more and more present in several fields. It is undeniable that many aspects of our society are empowered by such technologies: web searches, content filtering on social networks, recommendations on e-commerce websites, mobile applications, etc., in addition to academic research. Moreover, mobile devices and internet sites, e.g., social networks, support the collection and sharing of information in real time. The pervasive deployment of the aforementioned technological instruments, both hardware and software, has led to the production of huge amounts of data. Such data has become more and more unmanageable, posing challenges to conventional computing platforms, and paving the way to the development and widespread use of the machine and deep learning. Nevertheless, machine learning is not only a technology. Given a task, machine learning is a way of proceeding (a way of thinking), and as such can be approached from different perspectives (points of view). This, in particular, will be the focus of this research. The entire work concentrates on machine learning, starting from different sources of data, e.g., signals and images, applied to different domains, e.g., Sport Science and Social History, and analyzed from different perspectives: from a non-data scientist point of view through tools and platforms; setting a problem stage from scratch; implementing an effective application for classification tasks; improving user interface experience through Data Visualization and eXtended Reality. In essence, not only in a quantitative task, not only in a scientific environment, and not only from a data-scientist perspective, machine (and deep) learning can do the difference

    A robotic platform for precision agriculture and applications

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    Agricultural techniques have been improved over the centuries to match with the growing demand of an increase in global population. Farming applications are facing new challenges to satisfy global needs and the recent technology advancements in terms of robotic platforms can be exploited. As the orchard management is one of the most challenging applications because of its tree structure and the required interaction with the environment, it was targeted also by the University of Bologna research group to provide a customized solution addressing new concept for agricultural vehicles. The result of this research has blossomed into a new lightweight tracked vehicle capable of performing autonomous navigation both in the open-filed scenario and while travelling inside orchards for what has been called in-row navigation. The mechanical design concept, together with customized software implementation has been detailed to highlight the strengths of the platform and some further improvements envisioned to improve the overall performances. Static stability testing has proved that the vehicle can withstand steep slopes scenarios. Some improvements have also been investigated to refine the estimation of the slippage that occurs during turning maneuvers and that is typical of skid-steering tracked vehicles. The software architecture has been implemented using the Robot Operating System (ROS) framework, so to exploit community available packages related to common and basic functions, such as sensor interfaces, while allowing dedicated custom implementation of the navigation algorithm developed. Real-world testing inside the university’s experimental orchards have proven the robustness and stability of the solution with more than 800 hours of fieldwork. The vehicle has also enabled a wide range of autonomous tasks such as spraying, mowing, and on-the-field data collection capabilities. The latter can be exploited to automatically estimate relevant orchard properties such as fruit counting and sizing, canopy properties estimation, and autonomous fruit harvesting with post-harvesting estimations.Le tecniche agricole sono state migliorate nel corso dei secoli per soddisfare la crescente domanda di aumento della popolazione mondiale. I recenti progressi tecnologici in termini di piattaforme robotiche possono essere sfruttati in questo contesto. Poiché la gestione del frutteto è una delle applicazioni più impegnative, a causa della sua struttura arborea e della necessaria interazione con l'ambiente, è stata oggetto di ricerca per fornire una soluzione personalizzata che sviluppi un nuovo concetto di veicolo agricolo. Il risultato si è concretizzato in un veicolo cingolato leggero, capace di effettuare una navigazione autonoma sia nello scenario di pieno campo che all'interno dei frutteti (navigazione interfilare). La progettazione meccanica, insieme all'implementazione del software, sono stati dettagliati per evidenziarne i punti di forza, accanto ad alcuni ulteriori miglioramenti previsti per incrementarne le prestazioni complessive. I test di stabilità statica hanno dimostrato che il veicolo può resistere a ripidi pendii. Sono stati inoltre studiati miglioramenti per affinare la stima dello slittamento che si verifica durante le manovre di svolta, tipico dei veicoli cingolati. L'architettura software è stata implementata utilizzando il framework Robot Operating System (ROS), in modo da sfruttare i pacchetti disponibili relativi a componenti base, come le interfacce dei sensori, e consentendo al contempo un'implementazione personalizzata degli algoritmi di navigazione sviluppati. I test in condizioni reali all'interno dei frutteti sperimentali dell'università hanno dimostrato la robustezza e la stabilità della soluzione con oltre 800 ore di lavoro sul campo. Il veicolo ha permesso di attivare e svolgere un'ampia gamma di attività agricole in maniera autonoma, come l'irrorazione, la falciatura e la raccolta di dati sul campo. Questi ultimi possono essere sfruttati per stimare automaticamente le proprietà più rilevanti del frutteto, come il conteggio e la calibratura dei frutti, la stima delle proprietà della chioma e la raccolta autonoma dei frutti con stime post-raccolta

    Mobility classification of cattle with micro-Doppler radar

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    Lameness in dairy cattle is a welfare concern that negatively impacts animal productivity and farmer profitability. Micro-Doppler radar sensing has been previously suggested as a potential system for automating lameness detection in ruminants. This thesis investigates the refinement of the proposed automated system by analysing and enhancing the repeatability and accuracy of the existing scoring method in cattle mobility scoring, used to provide labels in machine learning. The main aims of the thesis were (1) to quantify the performance of the micro-Doppler radar sensing method for the assessment of mobility, (2) to characterise and validate micro-Doppler radar signatures of dairy cattle with varying degrees of gait impairment, and (3) to develop machine learning algorithms that can infer the mobility status of the animals under test from their radar signatures and support automatic contactless classification. The first study investigated inter-assessor agreement using a 4-level system and modifications to it, as well as the impact of factors such as mobility scoring experience, confidence in scoring decisions, and video characteristics. The results revealed low levels of agreement between assessors' scores, with kappa values ranging from 0.16 to 0.53. However, after transforming and reducing the mobility scoring system levels, an improvement was observed, with kappa values ranging from 0.2 to 0.67. Subsequently, a longitudinal study was conducted using good-agreement scores as ground truth labels in supervised machine-learning models. However, the accuracy of the algorithmic models was found to be insufficient, ranging from 0.57 to 0.63. To address this issue, different labelling systems and data pre-processing techniques were explored in a cross-sectional study. Nonetheless, the inter-assessor agreement remained challenging, with an average kappa value of 0.37 (SD = 0.16), and high-accuracy algorithmic predictions remained elusive, with an average accuracy of 56.1 (SD =16.58). Finally, the algorithms' performance was tested with high-confidence labels, which consisted of only scores 0 and 3 of the AHDB system. This testing resulted in good classification accuracy (0.82), specificity (0.79), and sensitivity (0.85). This led to the proposal of a new approach to producing labels, testing vantage point changes, and improving the performance of machine learning models (average accuracy = 0.7 & SD = 0.17, average sensitivity = 0.68 & SD = 0.27, average specificity = 0.75 & SD = 0.17). The research identified a challenge in creating high-confidence diagnostic labels for supervised machine learning-based algorithms to automate the detection and classification of lameness in dairy cows. As a result, the original goals were partially overridden, with the focus shifted to creating reliable labels that would perform well with radar data and machine learning. This point was considered necessary for smooth system development and process automation. Nevertheless, we managed to quantify the performance of the micro-Doppler radar system, partially develop the supervised machine learning algorithms, compare levels of agreement among multiple assessors, evaluate the assessment tools, assess the mobility evaluation process and gather a valuable data set which can be used as a foundation for subsequent studies. Finally, the thesis suggests changes in the assessment process to improve the prediction accuracy of algorithms based on supervised machine learning with radar data

    Computational Approaches Based On Image Processing for Automated Disease Identification On Chili Leaf Images: A Review

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    Chili, an important crop whose fruit is used as a spice, is significantly hampered by the existence of chili diseases. While these diseases pose a significant concern to farmers since they impair the supply of spices to the market, they can be managed and monitored to lessen their impact. Therefore, identifying chili diseases using a pertinent approach is of enormous importance. Over the years, the growth of computational approaches based on image processing has found its application in automated disease identification, leading to the availability of a reliable monitoring tool that produces promising findings for the chili. Numerous research papers on identifying chili diseases using the approaches have been published. Still, to the best knowledge of the author, there has not been a proper attempt to analyze these papers to describe the many steps of diagnosis, including pre-processing, segmentation, extraction of features, as well as identification techniques. Thus, a total of 50 research paper publications on the identification of chili diseases, with publication dates spanning from 2013 to 2021, are reviewed in this paper. Through the findings in this paper, it becomes feasible to comprehend the development trend for the application of computational approaches based on image processing in the identification of chili diseases, as well as the challenges and future directions that require attention from the present research community.&nbsp

    Computational Imaging for Phase Retrieval and Biomedical Applications

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    In conventional imaging, optimizing hardware is prioritized to enhance image quality directly. Digital signal processing is viewed as supplementary. Computational imaging intentionally distorts images through modulation schemes in illumination or sensing. Then its reconstruction algorithms extract desired object information from raw data afterwards. Co-designing hardware and algorithms reduces demands on hardware and achieves the same or even better image quality. Algorithm design is at the heart of computational imaging, with model-based inverse problem or data-driven deep learning methods as approaches. This thesis presents research work from both perspectives, with a primary focus on the phase retrieval issue in computational microscopy and the application of deep learning techniques to address biomedical imaging challenges. The first half of the thesis begins with Fourier ptychography, which was employed to overcome chromatic aberration problems in multispectral imaging. Then, we proposed a novel computational coherent imaging modality based on Kramers-Kronig relations, aiming to replace Fourier ptychography as a non-iterative method. While this approach showed promise, it lacks certain essential characteristics of the original Fourier ptychography. To address this limitation, we introduced two additional algorithms to form a whole package scheme. Through comprehensive evaluation, we demonstrated that the combined scheme outperforms Fourier ptychography in achieving high-resolution, large field-of-view, aberration-free coherent imaging. The second half of the thesis shifts focus to deep-learning-based methods. In one project, we optimized the scanning strategy and image processing pipeline of an epifluorescence microscope to address focus issues. Additionally, we leveraged deep-learning-based object detection models to automate cell analysis tasks. In another project, we predicted the polarity status of mouse embryos from bright field images using adapted deep learning models. These findings highlight the capability of computational imaging to automate labor-intensive processes, and even outperform humans in challenging tasks.</p
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