Microtubule-dependent ribosome localization in C. elegans neurons.

Subcellular localization of ribosomes defines the location and capacity for protein synthesis. Methods for in vivo visualizing ribosomes in multicellular organisms are desirable in mechanistic investigations of the cell biology of ribosome dynamics. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in Caenorhabditis elegans. Labeled ribosomes are detected as fluorescent puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. By examining mutants affecting axonal trafficking and performing a forward genetic screen, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons

The construction and operation of a water tunnel in application to flow visualization studies of an oscillating airfoil

The water tunnel which was constructed at the NASA Ames Research Center is described along with the flow field adjacent to an oscillating airfoil. The design and operational procedures of the tunnel are described in detail. Hydrogen bubble and thymol blue techniques are used to visualize the flow field. Results of the flow visualizations are presented in a series of still pictures and a high speed movie. These results show that time stall is more complicated than simple shedding from the leading edge or the trailing edge, particularly at relatively low frequency oscillations comparable to those of a helicopter blade. Therefore, any successful theory for predicting the stall loads on the helicopter blades must treat an irregular separated region rather than a discrete vortex passing over each blade surface

Effect of shadowing and diffraction on the received GNSS signal

Abstract. The characteristics of the GNSS received signal depend on the propagation medium environment. Typically, the transmitted signal interacts with several numbers of obstacles which leads to the multipath propagation to receiver end. In such cases, diffraction over the edges or wedges of the obstacles in the propagation path should be considered. The effects of diffraction and shadowing are the important elements in radio wave propagation because of their strong influence on the received signal quality. Especially, received signal strength and characteristics are very significant for GNSS applications. This master’s thesis investigates the effect of knife-edge diffraction model and shadowing environment on the received GNSS signal. Characterization of the received signal is investigated both theoretically and experimentally. In the measurement, a dual circular polarized antenna has been used to receive the multipath GPS signal in the shadow region in which antenna was placed in the zenith direction. The measurement was performed for two scenarios 1) the receiver was in the static position, and 2) in motion for the second measurement. Measurement results show that, the received signals following the Knife-edge diffraction pattern for both measurements case. In the first measurement case, knife-edge diffraction has been identified in received signal attenuation whereas the interference pattern in the LoS propagation can be observed because of multipath effect. In the deep shadow region, a linearly polarized signal has been received in both antennas because the incident RHCP signal turned into a linearly polarized signal after diffraction. For the second measurement case, knife-edge attenuation pattern is also visible. A clear agreement between the measured and theoretical aspects has been achieved in the case of knife-edge diffraction

Statistical channel modeling approach for professional wireless microphone systems

This thesis has been developed in order to extend a previous research [Vos08], in which a specific professional wireless microphone system (PWMS) application had been studied and analyzed. As a reminder, this PWMS application is based on the in ear monitoring IEM system which is composed by a fixed transmitting antenna and a body pack acting as a receiver. In [Vos08], real measurements of that application were taken inside the Hannover Congress Center hall. Data obtained were analyzed and modeled using the Saleh-Valenzuela channel model. This thesis extends that report, by using the WINNER channel model to simulate the same PWMS application.Escuela Técnica Superior de Ingeniería de Telecomunicació

Modeling and characterization of urban radio channels for mobile communications

Results of this thesis contribute in modeling and characterization of radio channels for future mobile communications. The results are presented mainly in three parts: a) modeling of propagation mechanisms, b) methodology of developing a propagation model, c) characterization of urban radio channel. One of the main propagation physical phenomena that have an important role in diverting signals to non line of sight scenarios is the diffraction process. This thesis proposes diffraction coefficients that have better agreement with finite difference time domain solution and rigorous diffraction theory than the coefficient commonly used in propagation predictions for mobile communications. The importance of diffuse scattering has also been investigated and showed that this physical process may have a key role in urban propagation, with a particular impact on the delay spread and angular spread of the signal at the receiver. This thesis proposes wideband propagation models for main and perpendicular streets of urban street grids. The propagation models are ray-based and are given in explicit mathematical expressions. Each ray is characterized in terms of its amplitude, delay, and angle of arrival, angle of departure for vertical and horizontal polarizations. Each of these characteristics is given in a closed mathematical form. Having wideband propagation model in explicit expression makes its implementation easy and computation fast. Secondary source modeling approach for perpendicular streets has also been introduced in this thesis. The last part of the thesis deals with characterization of urban radio channels for extracting parameters that help in successful design of mobile communication systems. Knowledge of channel characteristics enables reaching optimum trade off between system performance and complexity. This thesis analyzes measurement results at 2 GHz to extract channel parameters in terms of Rake finger characteristics in order to get information that helps to optimize Rake receiver design for enhanced-IMT2000 systems. Finger life distance has also been investigated for both micro- and small cell scenarios. This part of the thesis also presents orthogonality factor of radio channel for W-CDMA downlink at different bandwidths. Characterization of dispersion metrics in delay and angular domains for microcellular channels is also presented at different base station antenna heights. A measure of (dis-) similarity between multipath components in terms of separation distance in delay and angular domains is introduced by the concept of distance function, which is a step toward in development of algorithm extraction and analysis multipath clustering. In summary, the significant contributions of the thesis are in three parts. 1) Development of new diffraction coefficients and corrections of limitations of existing one for accurate propagation predictions for mobile communications. 2) Development of wideband propagation models for urban street grid. The novelty of the model is the development in explicit mathematical expressions. The developed models can be used to study propagation problem in microcellular urban street grids. 3) Presenting channel parameters that will help in the design of future mobile communication systems (enhanced-IMT2000), like number of active fingers, finger life distance, and orthogonality factors for different bandwidths. In addition, a technique based on multipath separation distance is proposed as a step toward in development of algorithms for extraction and analysis of multipath clusters.reviewe