Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural

Indexación: Web of Science; Scielo.RESUMEN. En Zonas Costeras rocosas, la desecación es gatillada porción Cambios Diarios en los Niveles de marea, y la Evidencia indica experimental de Me Distribución de las algas en la zona intermareal no está Relacionada estafa do palabra capacidad, párr tolerar la desecación. En Este Contexto, la Presencia de Pyropia columbina en la zona alta del intermareal sí Explica Por Su excepcional tolerancia fisiológica a la desecación. Este Estudio explora las Vías Metabólicas involucradas en la tolerancia a la desecación en P. columbina, un Través de la Caracterización de do transcriptoma Bajo Condiciones de hidratación contrastantes. Se obtuvó 1410 TER provenientes de dos Librerías de substracción de ADNc de frondas Naturalmente hidratadas y desecadas. Los transcriptomas de emba Librerías contienen transcritos de Diversas Rutas Metabólicas Relacionadas a la tolerancia. Entre el los transcritos expresados ​​15% estan involucrados en la Síntesis de Proteínas, do Procesamiento y degradacion, 14,4% Asociados un Fotosíntesis y cloroplasto, el 13,1% una mitocondrial Respiración and function, 10,6% al metabolism de la Pared Celular y 7,5% a la Actividad ANTIOXIDANTE, Proteínas chaperonas y factors de Defensa (catalasa, tiorredoxina, Proteínas de choque térmico, P450 citocromo). In Ambás Librerías sí DESTACA La Presencia De genes / Proteínas no descritos en algas. Proporciona Información This El Primer Trabajo molecular Que Estudia la tolerancia a desecación en P. columbina y Sus Resultados Ayudan a explicar los patrones clásicos de Distribución descritos párr algas en la zona intermareal. Palabras clave: Pyropia, desecación porción Estrés, EST, macroalgas, transcriptómica, Proteínas.ABSTRACT. In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.http://ref.scielo.org/jm5rc

Phylogenetics And Molecular Evolution Of Highly Eusocial Wasps

Societies where workers sacrifice their own reproduction and cooperatively nurture the offspring of a reproductive queen caste have originated repeatedly across the Tree of Life. The attainment of such reproductive division of labor enabled the evolution of remarkable diversity in development, behavior, and social organization in the Hymenoptera (ants, bees, and wasps). Wasps of the family Vespidae exhibit a gamut of social levels, ranging from solitary to highly social behavior. The highly social yellowjackets and hornets (Vespinae) have well developed differences in form and function between queens and workers, large colony sizes, and intricate nest architecture. Moreover, certain socially parasitic species in the Vespinae have secondarily lost the worker caste and rely entirely on the workers of a host species to ensure the survival of parasitic offspring. Understanding the evolution of behavioral traits in the Vespinae over long periods of time would be greatly enhanced by a robust hypothesis of historical relationships. In this study, I analyze targeted genes and transcriptomes to address three goals. First, infer phylogenetic relationships within yellowjackets (Vespula and Dolichovespula) and hornets (Vespa and Provespa). Second, test the hypothesis that social parasites are more closely related to their hosts than to any other species (Emery\u27s rule). Third, test the protein evolution hypothesis, which states that accelerated evolution of protein coding genes and positive selection operated in the transition to highly eusocial behavior. The findings of this study challenge the predominant understanding of evolutionary relationships in the Vespinae. I show that yellowjacket genera are not sister lineages, instead recovering Dolichovespula as more closely related to the hornets, and placing Vespula as sister to all other vespine genera. This implies that traits such as large colony size and high paternity are mostly restricted to a particular evolutionary trajectory (Vespula) from an early split in the Vespinae. I demonstrate that obligate and facultative social parasites do not share immediate common ancestry with their hosts, indicating that socially parasitic behavior likely evolved independently of host species. Moreover, obligate social parasites share a unique evolutionary history, suggesting that their parasitic behavior might have a genetic component. Lastly, I analyze transcriptomic data to infer a phylogeny of vespid wasps and use this phylogeny to discover lineage-specific signatures of positive selection. I identify more than two hundred genes showing signatures of positive selection on the branch leading to the highly eusocial yellowjackets and hornets. These positively selected genes involve functions related mainly to carbohydrate metabolism and mitochondrial activity, in agreement with insights from studies of bees and ants. Parallels of functional categories for genes under positive selection suggests that at the molecular level the evolution of highly eusocial behavior across the Hymenoptera might have followed similar and narrow paths

Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon

10.1186/1471-2164-8-120BMC Genomics8-BGME