Micro/Nano Manufacturing

Micro manufacturing involves dealing with the fabrication of structures in the size range of 0.1 to 1000 µm. The scope of nano manufacturing extends the size range of manufactured features to even smaller length scales—below 100 nm. A strict borderline between micro and nano manufacturing can hardly be drawn, such that both domains are treated as complementary and mutually beneficial within a closely interconnected scientific community. Both micro and nano manufacturing can be considered as important enablers for high-end products. This Special Issue of Applied Sciences is dedicated to recent advances in research and development within the field of micro and nano manufacturing. The included papers report recent findings and advances in manufacturing technologies for producing products with micro and nano scale features and structures as well as applications underpinned by the advances in these technologies

Polymer Processing and Surfaces

This book focuses on fundamental and applied research on polymer processing and its effect on the final surface as the optimization of polymer surface properties results in the unique applicability of these over other materials. The development and testing of the next generation of polymeric and composite materials is of particular interest. Special attention is given to polymer surface modification, external stimuli-responsive surfaces, coatings, adhesion, polymer and composites fatigue analysis, evaluation of the surface quality and microhardness, processing parameter optimization, characterization techniques, among others

BioMEMS

As technological advancements widen the scope of applications for biomicroelectromechanical systems (BioMEMS or biomicrosystems), the field continues to have an impact on many aspects of life science operations and functionalities. Because BioMEMS research and development require the input of experts who use different technical languages and come from varying disciplines and backgrounds, scientists and students can avoid potential difficulties in communication and understanding only if they possess a skill set and understanding that enables them to work at the interface of engineering and biosciences. Keeping this duality in mind throughout, BioMEMS: Science and Engineering Perspectives supports and expedites the multidisciplinary learning involved in the development of biomicrosystems. Divided into nine chapters, it starts with a balanced introduction of biological, engineering, application, and commercialization aspects of the field. With a focus on molecules of biological interest, the book explores the building blocks of cells and viruses, as well as molecules that form the self-assembled monolayers (SAMs), linkers, and hydrogels used for making different surfaces biocompatible through functionalization. The book also discusses: Different materials and platforms used to develop biomicrosystems Various biological entities and pathogens (in ascending order of complexity) The multidisciplinary aspects of engineering bioactive surfaces Engineering perspectives, including methods of manufacturing bioactive surfaces and devices Microfluidics modeling and experimentation Device level implementation of BioMEMS concepts for different applications. Because BioMEMS is an application-driven field, the book also highlights the concepts of lab-on-a-chip (LOC) and micro total analysis system (μTAS), along with their pertinence to the emerging point-of-care (POC) and point-of-need (PON) applications

Integrating Micro-Scale Separations to Matrix Assisted Laser Desorption and Ioniation Time of Flight Mass Spectrometry (MALDI-TOF-MS) for Protein Analysis

This dissertation describes the integration of micro-scale separations to matrix assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for protein analysis. MALDI MS provides unsurpassed accurate mass measurements of intact bio-molecules, for example peptides and proteins, which in turn generate high molecular specificity enabling the identity, function and structure of these molecules to be characterized. However, in order to realize the full potential of MS in proteomic studies, integrated sample processing on automated and high throughput platforms is required to address the complexity, diversity and the dynamic range of proteomic analysis. The work described here contributes towards the development of automated and high throughput micro-total analysis systems (µ-TAS) for proteomics. An overview of mass spectrometry instrumentation and techniques used in protein analysis is presented to highlight the significance of the work described. Microfluidics devices can serve as automated and high throughput platforms for integrating proteomics sample processing steps such as whole cell lyses, enrichment, solubilization, denaturation, protein separations, proteolytic digestion and chromatographic separations of peptides prior to MALDI TOF MS analysis. Therefore, coupling microfluidics devices to biological mass spectrometry is the first logical step towards developing fully integrated and automated systems for protein analysis. On-line and off-line approaches for analysis from microfluidic devices are discussed. The development of a specially tailored rotating ball inlet for automated on-line MALDI MS sample introduction from an electrophoresis-based separation platform is described. Electrophoresis-based micro-scale separations of peptides on fused silica capillary and polymer-based microfluidic devices were coupled to on-line MALDI TOF MS using a rotating ball inlet. The rotating ball inlet allowed for individual technique optimization and automation thereby eliminating the need for fractionation and routine MALDI sample preparation. High throughput solid phase micro-reactors for efficient enzymatic cleavages and improved protein identification with MALDI MS in a microfluidic device were also developed for incorporation in an integrated protein analysis microfluidic system. Future work that outlines the framework and focus geared towards integrating the modules discussed in this dissertation into a functional micro-total analysis system for protein sample processing is discussed

BioMEMS

As technological advancements widen the scope of applications for biomicroelectromechanical systems (BioMEMS or biomicrosystems), the field continues to have an impact on many aspects of life science operations and functionalities. Because BioMEMS research and development require the input of experts who use different technical languages and come from varying disciplines and backgrounds, scientists and students can avoid potential difficulties in communication and understanding only if they possess a skill set and understanding that enables them to work at the interface of engineering and biosciences. Keeping this duality in mind throughout, BioMEMS: Science and Engineering Perspectives supports and expedites the multidisciplinary learning involved in the development of biomicrosystems. Divided into nine chapters, it starts with a balanced introduction of biological, engineering, application, and commercialization aspects of the field. With a focus on molecules of biological interest, the book explores the building blocks of cells and viruses, as well as molecules that form the self-assembled monolayers (SAMs), linkers, and hydrogels used for making different surfaces biocompatible through functionalization. The book also discusses: Different materials and platforms used to develop biomicrosystems Various biological entities and pathogens (in ascending order of complexity) The multidisciplinary aspects of engineering bioactive surfaces Engineering perspectives, including methods of manufacturing bioactive surfaces and devices Microfluidics modeling and experimentation Device level implementation of BioMEMS concepts for different applications. Because BioMEMS is an application-driven field, the book also highlights the concepts of lab-on-a-chip (LOC) and micro total analysis system (μTAS), along with their pertinence to the emerging point-of-care (POC) and point-of-need (PON) applications

Microfluidics and Nanofluidics Handbook

The Microfluidics and Nanofluidics Handbook: Two-Volume Set comprehensively captures the cross-disciplinary breadth of the fields of micro- and nanofluidics, which encompass the biological sciences, chemistry, physics and engineering applications. To fill the knowledge gap between engineering and the basic sciences, the editors pulled together key individuals, well known in their respective areas, to author chapters that help graduate students, scientists, and practicing engineers understand the overall area of microfluidics and nanofluidics. Topics covered include Finite Volume Method for Numerical Simulation Lattice Boltzmann Method and Its Applications in Microfluidics Microparticle and Nanoparticle Manipulation Methane Solubility Enhancement in Water Confined to Nanoscale Pores Volume Two: Fabrication, Implementation, and Applications focuses on topics related to experimental and numerical methods. It also covers fabrication and applications in a variety of areas, from aerospace to biological systems. Reflecting the inherent nature of microfluidics and nanofluidics, the book includes as much interdisciplinary knowledge as possible. It provides the fundamental science background for newcomers and advanced techniques and concepts for experienced researchers and professionals

Future Trends in Advanced Materials and Processes

The Special Issue “Future Trends in Advanced Materials and Processes” contains original high-quality research papers and comprehensive reviews addressing the relevant state-of-the-art topics in the area of materials focusing on relevant or innovative applications such as radiological hazard evaluations of non-metallic materials, composite materials' characterization, geopolymers, metallic biomaterials, etc

Titanium and Its Alloys for Biomedical Applications

In recent decades, metals have been considered promising materials in the fields of regenerative medicine and tissue engineering. Metallic bio-materials with excellent mechanical strength can effectively support and replace damaged tissue. Hence, metals have been widely used in load-bearing applications for dentistry and orthopedics. Cobalt-, iron-, and titanium (Ti)-based alloys are representative bio-metals, which are used in various forms, such as vascular stents, hip joints, dental, and orthopedic implants. However, the alloying elements of Co- and Fe-based alloys, Co, Ni, and Cr, induce severe toxicity when ionized in the body, which limits their clinical use. However, Ti and its alloys have been widely used as medical devices and implants, with dental and orthopedic applications due to their excellent bone-regeneration ability, mechanical properties, and corrosion resistance. Even though Ti and its alloys have generally been used for biomedical applications, there are still challenges that must be met to satisfy their clinical application. For example, osseointegration with the surrounding bone tissue at the initial stage of implantation has been pointed to as a major issue. This Special Issue, “Titanium and Its Alloys for Biomedical Applications”, has been proposed to present recent developments in biomedical applications. The nine research articles included in this Special Issue cover broad aspects of Ti-based alloys and composites with respect to their composition, mechanical, and biological properties, as highlighted in this editorial

Diffuse Reflectance Spectroscopy to Quantify In Vivo Tissue Optical Properties: Applications in Human Epithelium and Subcutaneous Murine Colon Cancer

Colorectal cancer is the 4th most common and 2nd deadliest cancer. Problems exist with predicting which patients will respond best to certain therapy regimens. Diffuse reflectance spectroscopy has been suggested as a candidate to optically monitor a patient’s early response to therapy and has been received favorably in experimentally managing other cancers such as breast and skin. In this dissertation, two diffuse reflectance spectroscopy probes were designed: one with a combined high-resolution microendoscopy modality, and one that was optimized for acquiring data from subcutaneous murine tumors. For both probes, percent errors for estimating tissue optical properties (reduced scattering coefficient and absorption coefficient) were less than 5% and 10%, respectively. Then, studies on tissue-simulating phantoms were performed to test probe sensitivity and to serve as testing platforms for investigators in biomedical optics. Next, the diffuse reflectance spectroscopy probe was applied to subcutaneous murine colon tumors (n=61) undergoing either antibody immunotherapy or standard 5-fluorouracil chemotherapy. Mice treated with a combination of these therapies showed reduced tumor growth compared to saline control, isotype control, immunotherapy, and chemotherapy groups (p\u3c0.001, \u3c0.001, \u3c0.001, and 0.046, respectively) 7 days post-treatment. Additionally, at 7 days post-treatment, oxyhemoglobin, a marker currently being explored as a functional prognostic cancer marker, trended to increase in immunotherapy, chemotherapy, and combination therapy groups compared to controls (p=0.315, 0.149, and 0.190). Also of interest, an oxyhemoglobin flare (averageincrease of 1.44x from baseline, p=0.03 compared to controls) was shown in tumors treated with chemotherapy, indicating that diffuse reflectance spectroscopy may be useful as a complimentary tool to monitor early tumor therapeutic response in colon cancer. However, subject-to-subject variability was high and studies correlating survival to early oxyhemoglobin flares are suggested