Statistical optimization of culture medium for yellow pigment production by Thermomyces sp.

In present study, Thermomyces sp. were able to produce high yield of yellow pigments screened. Pigment production by Thermomyces sp was optimized by employing factorial design and response surface techniques in submerged fermentation. The variables evaluated were the concentrations of, sucrose, yeast extract, ammonium sulphate, magnesium sulphate and dipotassium hydrogen phosphate having as response pigment production. One factor at-a-time method was employed for the optimization of media components. Response surface methodology (RSM) optimized these nutrient parameters for maximum yellow pigment production (1387 OD units), which resulted at 35.5 g/L sucrose 5.5 g/L yeast extract, 2.5 g/L NH4SO4, 0.3 g/L MgSO4 and 1.0 g/L K2HPO4 in the medium. Response surface methodology (RSM) was further used to determine the optimum values of process variables for maximum yellow pigment production. The fit of the quadratic model was found to be significant. A significant increase in yellow pigment production was achieved using RSM

Kemijski sastav sojeva plijesni Monascus ruber

Chemical profile of Monascus ruber strains has been studied using gas chromatography-mass spectrometry (GC/MS) analysis. The colour intensity of the red pigment and secondary metabolic products of two M. ruber strains (AUMC 4066 and AUMC 5705) cultivated on ten different media were also studied. Metabolic products can be classified into four categories: anticholesterol, anticancer, food colouring, and essential fatty acids necessary for human health. Using GC/MS, the following 88 metabolic products were detected: butyric acid and its derivatives (25 products), other fatty acids and their derivatives (19 products), pyran and its derivatives (22 products) and other metabolites (22 products). Among these, 32 metabolites were specific for AUMC 4066 strain and 34 for AUMC 5705 strain, whereas 22 metabolites were produced by both strains on different tested substrates. Production of some metabolites depended on the substrate used. High number of metabolites was recorded in the red pigment extract obtained by both strains grown on malt broth and malt agar. Also, 42 aroma compounds were recorded (4 alcohols, 2 benzaldehydes, 27 esters, 3 lactones, 1 phenol, 1 terpenoid, 3 thiol compounds and acetate-3-mercapto butyric acid). Thin layer chromatography and GC/MS analyses revealed no mycotoxin citrinin in any media used for the growth of the two M. ruber strains.Svrha je rada bila proučiti kemijski sastav sojeva plijesni Monascus ruber pomoću plinske kromatografije i masene spektrometrije. Ispitan je i intenzitet crvenog pigmenta te sekundarni produkti metabolizma dvaju sojeva plijesni M. ruber (AUMC 4066 i AUMC 5705) uzgojenih na deset različitih podloga. Produkti metabolizma mogu se razvrstati u 4 skupine: spojevi koji snižavaju razinu kolesterola, antikancerogeni spojevi, prehrambene boje i esencijalne masne kiseline neophodne za ljudsko zdravlje. Plinskom je kromatografijom i masenom spektrometrijom pronađeno 88 produkata metabolizma, a među njima maslačna kiselina i njezini derivati (25), ostale masne kiseline i njihovi derivati (19), piran i njegovi derivati (22), te ostali metaboliti (22). Od ukupnog broja produkata, njih 33 je specifično za soj AUMC 4066, a 34 za soj AUMC 5705, dok su oba soja proizvela 22 produkta na različitim podlogama. Proizvodnja je produkata ovisila o upotrijebljenoj podlozi. Velik je broj metabolita pronađen u ekstraktima crvenog pigmenta iz oba soja uzgojena na podlogama od slada. Pronađena su 42 aromatska spoja, i to 4 alkohola, 27 estera, 3 laktona, 1 fenol, 1 terpenoid, 3 tiola i 1 merkapto spoj. Tankoslojnom i plinskom kromatografijom te masenom spektrometrijom nije pronađen mikotoksin citrinin ni u jednoj podlozi na kojoj su rasla ova dva soja plijesni M. ruber

Development of a Novel Restrictive Medium for Monascus Enrichment From Hongqu Based on the Synergistic Stress of Lactic Acid and Ethanol

Hongqu is a famous fermented food produced by Monascus and has been used as food coloring, wine starters and food additives for thousands of years in China. Excellent Monascus strain is an important prerequisite for producing high-quality Hongqu. However, the isolation of Monascus pure culture from Hongqu samples is time-consuming and laborious because it is easily interfered by other microorganisms (especially filamentous fungi). Therefore, the development of restrictive medium for Monascus enrichment from Hongqu is of great significance for the preparation and screening of excellent Monascus strains. Results of this study showed that Monascus has good tolerance to lactic acid and ethanol. Under the conditions of tolerance limits [7.5% lactic acid (v/v) and 12.0% ethanol (v/v)], Monascus could not grow but it still retained the vitality of spore germination, and the spore activity gradually decreased with the increasing concentrations of lactic acid and ethanol. More interestingly, the addition of lactic acid and ethanol significantly changed the microbial community structure in rice milk inoculated with Hongqu. After response surface optimization, Monascus could be successfully enriched without the interference of other microorganisms when 3.98% (v/v) lactic acid and 6.24% (v/v) ethanol were added to rice milk simultaneously. The optimal enrichment duration of Monascus by the restrictive medium based on the synergistic stress of lactic acid and ethanol is 8∼24 h. The synergistic stress of lactic acid and ethanol had no obvious effects on the accumulation of major metabolites in the progeny of Monascus, and was suitable for the enrichment of Monascus from different types of Hongqu. Finally, the possible mechanisms on the tolerance of Monascus to the synergistic stress of lactic acid and ethanol were preliminarily studied. Under the synergistic stress of lactic acid and ethanol, the cell membrane of Monascus defends against lactic acid and ethanol into cells to some extent, and the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities of Monascus were higher than those of other fungi, which significantly reduced the degree of lipid peroxidation of cell membrane, while secreting more amylase to make reducing sugars to provide the cells with enough energy to resist environmental stress. This work has great application value for the construction of Monascus strain library and the better development of its germplasm resources

Fungi and Fungal Metabolites for the Improvement of Human and Animal Nutrition and Health

The purpose of this book was not to provide a comprehensive overview of the vast arena of how fungi and fungal metabolites are able to improve human and animal nutrition and health; rather, we, as Guest Editors, wished to encourage authors working in this field to publish their most recent work in this rapidly growing journal in order for the large readership to appreciate the full potential of wonderful and beneficial fungi. Thus, this Special Issue welcomed scientific contributions on applications of fungi and fungal metabolites, such as bioactive fatty acids, pigments, polysaccharides, alkaloids, terpenoids, etc., with great potential in human and animal nutrition and health

Microbial Secondary Metabolites and Biotechnology

Many research teams are working to demonstrate that microorganisms can be our daily partners, due to the great diversity of biochemical transformations and molecules they are able to produce. This Special Issue highlights several facets of the production of microbial metabolites of interest. From the discovery of new strains or new bioactive molecules issued from novel environments, to the increase in their synthesis by traditional or innovative methods, different levels of biotechnological processes are addressed. Combining the new dimensions of "Omics" sciences, such as genomics, transcriptomics or metabolomics, microbial biotechnologies are opening up incredible opportunities for discovering and improving microorganisms and their production

Última generación de sistemas luminiscentes multiconmutados en flujo con aplicación en los campos agroalimentario y farmacológico

En esta Memoria se proponen una serie de mejoras científico-técnicas relativas al desarrollo y aplicación de sistemas automatizados en flujo para el análisis de moléculas de diferente índole en los campos farmacológico y agroalimentario, plasmadas en las nueve publicaciones científicas que se presentan, mostrando su versatilidad y aplicabilidad analítica. Se ha puesto especial hincapié en incrementar el nivel de automatización de los sensores en flujo descritos hasta la fecha, perfeccionando el uso de los sistemas multiconmutados en flujo en este tipo de sistemas analíticos. Se han empleado técnicas de detección luminiscentes, incidiendo especialmente en aquellas que han permitido un incremento en la selectividad y cuyo desarrollo hasta la fecha ha sido reducido, como es el caso de la Fluorescencia Inducida Fotoquímicamente. Igualmente, se han utilizado nanopartículas fluorescentes para poder llevar a cabo la determinación luminiscente de analitos que no presentan fluorescencia nativa.In this Report, several scientific/technical improvements concerning the development and the application of automated flow systems are proposed. The systems developed were applied to the analysis of molecules of different nature in the pharmacological and agri-food fields, showing its versatility and its analytical applicability. The research here described gave origin to nine scientific publications in scientific international journals. Special emphasis was placed on increasing the level of automation of the flow sensors described to date, particularly improving the potential of multicommutated flow injection analysis methods. The selection of luminescent detection techniques was performed by paying special attention to two aspects: a) increasing the selectivity of the analytical methods; b) the use of detection techniques that have been underused in flow systems, such as Photochemically Induced Fluorescence. Likewise, fluorescent nanoparticles have been used to carry out the luminescent determination of analytes that do not exhibit native fluorescence.Tesis Univ. Jaén. Departamento Química Física y Analítica. Leída el 26 de noviembre de 2018

XII CIBIA Iberoamerican congress of food engineering: challenging food engineering as a driver towards sustainable food processing - Book of abstracts

The XII edition of CIBIA, Iberoamerican Congress of Food Engineering will take place, for the first time, in Portugal next July 1 to 4, having as theme “Challenging Food Engineering as a Driver Towards Sustainable Food Processing“. The host institution is the University of Algarve, Faro, in the Algarve region. The importance of the Food Engineering field was enhanced by the number of Iberoamerican researchers who participated in this XIIth edition of CIBIA. We received 530 abstracts submitted by researchers from the Iberian Peninsula (Portugal and Spain), and from all the South and Central American Continent: Brazil, Chile, Peru, Mexico, Colombia and Ecuador, among others. About 350 participants will be present with around 100 accepted abstracts for oral presentations and more than 300 poster presentations. These numbers reveal that Food Engineering is very alive and strong in the Iberoamerican world as many young researchers are pursuing their graduate studies in various disciplines of this field and senior researchers keep facing and studying new challenges such as the main Congress Theme “Challenging Food Engineering as a Driver towards Sustainable Food Processing”.info:eu-repo/semantics/publishedVersio

Selected Papers from the 5th International Symposium on Mycotoxins and Toxigenic Moulds: Challenges and Perspectives

The MYTOX association research platform, MYTOX "Mycotoxins and Toxigenic Moulds" was established in 2007 and consists of more than 50 researchers from 12 research laboratories in the Ghent University Association. MYTOX deals with mycotoxin research in a multi-disciplinary way, based on four main themes: (1) mycotoxins; (2) toxigenic fungi; (3) mycotoxins and animal health; and (4) mycotoxins and human health. In this way, MYTOX tackles the mycotoxin issue along the production chain from the field to the end consumer, within the ‘One Health’ concept. We welcomed over 100 scientists, researchers and representatives from industry, government as well as academia at the 5th edition of the MYTOX International Symposium, held in Ghent on May 11, 2016. The program included oral and poster presentations related to fungal-related disease monitoring and mycotoxin production; mycotoxin analysis in food and feed, as well as in animal and human biological samples; prevention on the field; management strategies during food and feed storage and processing

Research on Characterization and Processing of Table Olives

Written by experts in the field of table olives, this book is a source of recent research advances on the characterization and processing of table olives. Research papers are provided relating to the characterization of their composition of volatiles and the sensory profile; mineral composition and bioavailability; changes in bioactive components (chlorophylls) by processing; and new strategies to reduce sodium and additives for stabilizing the organoleptic properties and avoiding defects in table olives. Other research papers are included in relation to microbiological and chemical changes in table olives during spontaneous or controlled fermentation employing different cultivars, and the optimized use of starter cultures for the improvement of the different fermentative processes. In addition, this book includes an overview of the main technologies used for olive fermentation, including the role of lactic acid bacteria and yeasts characterizing this process, and of the processing and storage effects on the nutritional and sensory properties of table olives