2,802 research outputs found

    Understanding/unravelling carotenoid excited singlet states.

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    Carotenoids are essential light-harvesting pigments in natural photosynthesis. They absorb in the blue–green region of the solar spectrum and transfer the absorbed energy to (bacterio-)chlorophylls, and thus expand the wavelength range of light that is able to drive photosynthesis. This process is an example of singlet–singlet excitation energy transfer, and carotenoids serve to enhance the overall efficiency of photosynthetic light reactions. The photochemistry and photophysics of carotenoids have often been interpreted by referring to those of simple polyene molecules that do not possess any functional groups. However, this may not always be wise because carotenoids usually have a number of functional groups that induce the variety of photochemical behaviours in them. These differences can also make the interpretation of the singlet excited states of carotenoids very complicated. In this article, we review the properties of the singlet excited states of carotenoids with the aim of producing as coherent a picture as possible of what is currently known and what needs to be learned

    Do Water Molecules Displaced by Hydrophobic Interactions Stabilize Antigen-Antibody Binding? Physico-chemical background of antigen-antibody reactions analyzed by fluorescent and Fourier-transform infrared spectroscopy on FITC – anti-FITC (IgG1) model

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    BACKGROUND: Antigen-antibody reactions are a special field of molecular interactions. The physico-chemical nature of antigen-antibody binding and ligand-induced changes in the fine molecular structures of antigens during immunocomplex formation are less studied. However, these changes in the molecular appearance are extremely important for further molecular recognition. The major aim of this study is to clarify the physico-chemical modification of the antigen/hapten during immunobinding using model experiments. METHODS: An appropriate model system was designed for our investigations: fluorescein-iso-thiocyanate (FITC, isomer I) was used as the antigen (hapten), and its interactions with a specific antibody (monoclonal anti-FITC IgG1) were analyzed using spectrophotometry, different spectrofluorimetric methods and fluorescence polarization, and Fourier-transform infrared spectroscopic methods. RESULTS: Fluorescent polarization and infrared spectroscopic measurements detected a local decrease in the hydration degree in the submolecular area of the specific ligand between the small antigen (hapten) molecule and the hypervariable region of the specific IgG1, causing “rigidization” of molecular movements. Changes in hydration modified the molecular microenvironment, allowing them to influence further functions of both immunoglobulins and the antigen. CONCLUSION: Hydrophobic interactions with exclusion of water molecules around the binding sites seem to be thermodynamically strong enough for stable molecular binding without a covalent chemical interaction between the antigen and the antibody. The results of this study, together with data obtained in previous research, help understand the molecular dynamics of the antigen-antibody reaction better

    Review on bibliography related to antimicrobials

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    In this report, a bibliographic research has been done in the field of antimicrobials.In this report, a bibliographic research has been done in the field of antimicrobials. Not all antimicrobials have been included, but those that are being subject of matter in the group GBMI in Terrassa, and others of interest. It includes chitosan and other biopolymers. The effect of nanoparticles is of great interest, and in this sense, the effect of Ag nanoparticles and antibiotic nanoparticles (nanobiotics) has been revised. The report focuses on new publications and the antimicrobial effect of peptides has been considered. In particular, the influence of antimicrobials on membranes has deserved much attention and its study using the Langmuir technique, which is of great utility on biomimetic studies. The building up of antimicrobials systems with new techniques (bottom-up approach), as the Layer-by-Layer technique, can also be found in between the bibliography. It has also been considered the antibiofilm effect, and the new ideas on quorem sensing and quorum quenching.Preprin

    Optical code-division multiple access system and optical signal processing

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    This thesis presents our recent researches on the development of coding devices, the investigation of security and the design of systems in the optical cod-division multiple access (OCDMA) systems. Besides, the techniques of nonlinear signal processing used in the OCDMA systems fire our imagination, thus some researches on all-optical signal processing are carried out and also summarized in this thesis. Two fiber Bragg grating (FBG) based coding devices are proposed. The first coding device is a superstructured FBG (SSFBG) using ±π/2-phase shifts instead of conventional 0/π-phase shifts. The ±π/2-phase-shifted SSFBG en/decoders can not only conceal optical codes well in the encoded signals but also realize the reutilization of available codes by hybrid use with conventional 0/π-phase-shifted SSFBG en/decoders. The second FBG based coding device is synthesized by layer-peeling method, which can be used for simultaneous optical code recognition and chromatic dispersion compensation. Then, two eavesdropping schemes, one-bit delay interference detection and differential detection, are demonstrated to reveal the security vulnerability of differential phase-shift keying (DPSK) and code-shift keying (CSK) OCDMA systems. To address the security issue as well as increase the transmission capacity, an orthogonal modulation format based on DPSK and CSK is introduced into the OCDMA systems. A 2 bit/symbol 10 Gsymbol/s transmission system using the orthogonal modulation format is achieved. The security of the system can be partially guaranteed. Furthermore, a fully-asynchronous gigabit-symmetric OCDMA passive optical network (PON) is proposed, in which a self-clocked time gate is employed for signal regeneration. A remodulation scheme is used in the PON, which let downstream and upstream share the same optical carrier, allowing optical network units source-free. An error-free 4-user 10 Gbit/s/user duplex transmission over 50 km distance is reazlied. A versatile waveform generation scheme is then studied. A theoretical model is established and a waveform prediction algorithm is summarized. In the demonstration, various waveforms are generated including short pulse, trapezoidal, triangular and sawtooth waveforms and doublet pulse. ii In addition, an all-optical simultaneous half-addition and half-subtraction scheme is achieved at an operating rate of 10 GHz by using only two semiconductor optical amplifiers (SOA) without any assist light. Lastly, two modulation format conversion schemes are demonstrated. The first conversion is from NRZ-OOK to PSK-Manchester coding format using a SOA based Mach-Zehnder interferometer. The second conversion is from RZ-DQPSK to RZ-OOK by employing a supercontinuum based optical thresholder

    Stress assessment based on EEG univariate features and functional connectivity measures

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    The biological response to stress originates in the brain but involves different biochemical and physiological effects. Many common clinical methods to assess stress are based on the presence of specific hormones and on features extracted from different signals, including electrocardiogram, blood pressure, skin temperature, or galvanic skin response. The aim of this paper was to assess stress using EEG-based variables obtained from univariate analysis and functional connectivity evaluation. Two different stressors, the Stroop test and sleep deprivation, were applied to 30 volunteers to find common EEG patterns related to stress effects. Results showed a decrease of the high alpha power (11 to 12 Hz), an increase in the high beta band (23 to 36 Hz, considered a busy brain indicator), and a decrease in the approximate entropy. Moreover, connectivity showed that the high beta coherence and the interhemispheric nonlinear couplings, measured by the cross mutual information function, increased significantly for both stressors, suggesting that useful stress indexes may be obtained from EEG-based features.Peer ReviewedPostprint (author’s final draft
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