44,963 research outputs found
Construction and abstraction: contrasting methods of supporting model building in learning science
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Ab Initio Modeling of Ecosystems with Artificial Life
Artificial Life provides the opportunity to study the emergence and evolution
of simple ecosystems in real time. We give an overview of the advantages and
limitations of such an approach, as well as its relation to individual-based
modeling techniques. The Digital Life system Avida is introduced and prospects
for experiments with ab initio evolution (evolution "from scratch"),
maintenance, as well as stability of ecosystems are discussed.Comment: 13 pages, 2 figure
Metagenomic sequencing unravels gene fragments with phylogenetic signatures of O2-tolerant NiFe membrane-bound hydrogenases in lacustrine sediment
Many promising hydrogen technologies utilising hydrogenase enzymes have been slowed by the fact that most hydrogenases are extremely sensitive to O2. Within the group 1 membrane-bound NiFe hydrogenase, naturally occurring tolerant enzymes do exist, and O2 tolerance has been largely attributed to changes in iron–sulphur clusters coordinated by different numbers of cysteine residues in the enzyme’s small subunit. Indeed, previous work has provided a robust phylogenetic signature of O2 tolerance [1], which when combined with new sequencing technologies makes bio prospecting in nature a far more viable endeavour. However, making sense of such a vast diversity is still challenging and could be simplified if known species with O2-tolerant enzymes were annotated with information on metabolism and natural environments. Here, we utilised a bioinformatics approach to compare O2-tolerant and sensitive membrane-bound NiFe hydrogenases from 177 bacterial species with fully sequenced genomes for differences in their taxonomy, O2 requirements, and natural environment. Following this, we interrogated a metagenome from lacustrine surface sediment for novel hydrogenases via high-throughput shotgun DNA sequencing using the Illumina™ MiSeq platform. We found 44 new NiFe group 1 membrane-bound hydrogenase sequence fragments, five of which segregated with the tolerant group on the phylogenetic tree of the enzyme’s small subunit, and four with the large subunit, indicating de novo O2-tolerant protein sequences that could help engineer more efficient hydrogenases
Speciesism, identity politics and ecocriticism : a conversation with humanists and posthumanists
An electronic conversation between 7 scholars from the fields of animal studies and early modern studies aimed at confronting "speciesism," and constructing what Cary Wolfe calls a "posthumanist theory of the subject.
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