Development of a chicken 5 K microarray targeted towards immune function

<p>Abstract</p> <p>Background</p> <p>The development of microarray resources for the chicken is an important step in being able to profile gene expression changes occurring in birds in response to different challenges and stimuli. The creation of an immune-related array is highly valuable in determining the host immune response in relation to infection with a wide variety of bacterial and viral diseases.</p> <p>Results</p> <p>Here we report the development of chicken immune-related cDNA libraries and the subsequent construction of a microarray containing 5190 elements (in duplicate). Clones on the array originate from tissues known to contain high levels of cells related to the immune system, namely Bursa, Peyers patch, thymus and spleen. Represented on the array are genes that are known to cluster with existing chicken ESTs as well as genes that are unique to our libraries. Some of these genes have no known homologies and represent novel genes in the chicken collection. A series of reference genes (ie. genes of known immune function) are also present on the array. Functional annotation data is also provided for as many of the genes on the array as is possible.</p> <p>Conclusion</p> <p>Six new chicken immune cDNA libraries have been created and nearly 10,000 sequences submitted to GenBank [GenBank: <ext-link ext-link-type="gen" ext-link-id="AM063043">AM063043</ext-link>-<ext-link ext-link-type="gen" ext-link-id="AM071350">AM071350</ext-link>; <ext-link ext-link-type="gen" ext-link-id="AM071520">AM071520</ext-link>-<ext-link ext-link-type="gen" ext-link-id="AM072286">AM072286</ext-link>; <ext-link ext-link-type="gen" ext-link-id="AM075249">AM075249</ext-link>-<ext-link ext-link-type="gen" ext-link-id="AM075607">AM075607</ext-link>]. A 5 K immune-related array has been developed from these libraries. Individual clones and arrays are available from the ARK-Genomics resource centre.</p

The prevalence of headache may be related with the latitude: a possible role of Vitamin D insufficiency?

According to recent observations, there is worldwide vitamin D insufficiency (VDI) in various populations. A number of observations suggest a link between low serum levels of vitamin D and higher incidence of chronic pain. A few case reports have shown a beneficial effect of vitamin D therapy in patients with headache disorders. Serum vitamin D level shows a strong correlation with the latitude. Here, we review the literature to delineate a relation of prevalence rate of headaches with the latitude. We noted a significant relation between the prevalence of both tension-type headache and migraine with the latitude. There was a tendency for headache prevalence to increase with increasing latitude. The relation was more obvious for the lifetime prevalence for both migraine and tension-type headache. One year prevalence for migraine was also higher at higher latitude. There were limited studies on the seasonal variation of headache disorders. However, available data indicate increased frequency of headache attacks in autumn–winter and least attacks in summer. This profile of headache matches with the seasonal variations of serum vitamin D levels. The presence of vitamin D receptor, 1α-hydroxylase and vitamin D-binding protein in the hypothalamus further suggest a role of vitamin D deficiency in the generation of head pain

The role of dark matter in the galaxy mass-size relationship

The observed relationship between stellar mass and effective radius for early type galaxies, pointed out by many authors, is interpreted in the context of Clausius' virial maximum theory. In this view, it is strongly underlined that the key of the above mentioned correlation is owing to the presence of a deep link between cosmology and the existence of the galaxy Fundamental Plane. Then the ultimate meaning is: understanding visible mass - size correlation and/or Fundamental Plane means understanding how galaxies form. The mass - size relationship involves baryon (mainly stellar) mass and its typical dimension related to the light, but it gets memory of the cosmological mass variance at the equivalence epoch. The reason is that the baryonic component virializes by sharing virial energy in about equal amount between baryons and dark matter, this sharing depending, in turn, on the steepness of the dark matter distribution. The general strategy consists in using the two-component tensor virial theorem for determining the virialized baryonic configurations. A King and a Zhao density profile are assumed for the inner baryonic and the outer dark matter component, respectively, at the end of the relaxation phase. All the considerations are restricted to spherical symmetry for simplicity. The effect of changing the dark-to-baryon mass ratio, m, is investigated inside a LambdaCDM scenario. A theoretical mass - size relation is expressed for the baryonic component, which fits fairly well to the data from a recently studied galaxy sample. Finally, the play of intrinsic dispersion on the mass ratio, m, is discussed in the light of the cusp/core problem and some consequences are speculated about the existence of a limit, m_l, expected by the theory.Comment: 36 pages, 8 figures (Accepted for publication in New Astronomy

Using the general link transmission model in a dynamic traffic assignment to simulate congestion on urban networks

This article presents two new models of Dynamic User Equilibrium that are particularly suited for ITS applications, where the evolution of vehicle flows and travel times must be simulated on large road networks, possibly in real-time. The key feature of the proposed models is the detail representation of the main congestion phenomena occurring at nodes of urban networks, such as vehicle queues and their spillback, as well as flow conflicts in mergins and diversions. Compared to the simple word of static assignment, where only the congestion along the arc is typically reproduced through a separable relation between vehicle flow and travel time, this type of DTA models are much more complex, as the above relation becomes non-separable, both in time and space. Traffic simulation is here attained through a macroscopic flow model, that extends the theory of kinematic waves to urban networks and non-linear fundamental diagrams: the General Link Transmission Model. The sub-models of the GLTM, namely the Node Intersection Model, the Forward Propagation Model of vehicles and the Backward Propagation Model of spaces, can be combined in two different ways to produce arc travel times starting from turn flows. The first approach is to consider short time intervals of a few seconds and process all nodes for each temporal layer in chronological order. The second approach allows to consider long time intervals of a few minutes and for each sub-model requires to process the whole temporal profile of involved variables. The two resulting DTA models are here analyzed and compared with the aim of identifying their possible use cases. A rigorous mathematical formulation is out of the scope of this paper, as well as a detailed explanation of the solution algorithm. The dynamic equilibrium is anyhow sought through a new method based on Gradient Projection, which is capable to solve both proposed models with any desired precision in a reasonable number of iterations. Its fast convergence is essential to show that the two proposed models for network congestion actually converge at equilibrium to nearly identical solutions in terms of arc flows and travel times, despite their two diametrical approaches wrt the dynamic nature of the problem, as shown in the numerical tests presented here

TURTLE-P: a UML profile for the formal validation of critical and distributed systems

The timed UML and RT-LOTOS environment, or TURTLE for short, extends UML class and activity diagrams with composition and temporal operators. TURTLE is a real-time UML profile with a formal semantics expressed in RT-LOTOS. Further, it is supported by a formal validation toolkit. This paper introduces TURTLE-P, an extended profile no longer restricted to the abstract modeling of distributed systems. Indeed, TURTLE-P addresses the concrete descriptions of communication architectures, including quality of service parameters (delay, jitter, etc.). This new profile enables co-design of hardware and software components with extended UML component and deployment diagrams. Properties of these diagrams can be evaluated and/or validated thanks to the formal semantics given in RT-LOTOS. The application of TURTLE-P is illustrated with a telecommunication satellite system

Model independent approach to studies of the confining dual Abrikosov vortex in SU(2) lattice gauge theory

We address the problem of determining the type I, type II or borderline dual superconductor behavior in maximal Abelian gauge SU(2) through the study of the dual Abrikosov vortex. We find that significant electric currents in the simulation data call into question the use of the dual Ginzburg Landau Higgs model in interpreting the data. Further, two definitions of the penetration depth parameter take two different values. The splitting of this parameter into two is intricately connected to the existence of electric currents. It is important in our approach that we employ definitions of flux and electric and magnetic currents that respect Maxwell equations exactly for lattice averages independent of lattice spacings. Applied to specific Wilson loop sizes, our conclusions differ from those that use the dual GLH model.Comment: 18 pages, 14 figures, change title, new anaylysis with more figure