7,382 research outputs found

    The effect of monitoring complexity on stakeholder acceptance of CO2 geological storage projects in the US gulf coast region

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    Environmental monitoring at geologic CO2 storage sites is required by regulations for the purposes of environmental protection and emissions accounting in the case of leakage to surface. However, another very important goal of environmental monitoring is to assure stakeholders that the project is monitored for safety and effectiveness. With current efforts to optimize monitoring for cost-effectiveness, the question remains: will optimization of monitoring approaches degrade stakeholder assurance, or do heavily-instrumented sites communicate higher risk to a stakeholder? We report the results of a stakeholder survey in Gulf Coast states of the US where carbon capture and storage (CCS) is developing quickly. We rely on a 2 by 2 factorial experiment in which we manipulate message complexity (complex v. simple) and social norm (support from scientists v. support from community members). Subjects were randomly assigned to one of four conditions: 1) complex message with scientist support; 2) complex message with community member support; 3) simple message with scientist support; or 4) simple message with community member support. In addition to the experimental stimuli, subjects were also asked about their need for cognition, attitudes toward science and scientists, attitudes about climate change and support for carbon capture and storage (CCS). Our sample is drawn from residents in states bordering the western Gulf of Mexico (Texas, Louisiana, Florida) where CO2 geologic storage is being planned both onshore and offshore. The results offer important implications for public outreach efforts to key stakeholders

    Single-cell time-series analysis of metabolic rhythms in yeast

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    The yeast metabolic cycle (YMC) is a biological rhythm in budding yeast (Saccharomyces cerevisiae). It entails oscillations in the concentrations and redox states of intracellular metabolites, oscillations in transcript levels, temporal partitioning of biosynthesis, and, in chemostats, oscillations in oxygen consumption. Most studies on the YMC have been based on chemostat experiments, and it is unclear whether YMCs arise from interactions between cells or are generated independently by each cell. This thesis aims at characterising the YMC in single cells and its response to nutrient and genetic perturbations. Specifically, I use microfluidics to trap and separate yeast cells, then record the time-dependent intensity of flavin autofluorescence, which is a component of the YMC. Single-cell microfluidics produces a large amount of time series data. Noisy and short time series produced from biological experiments restrict the computational tools that are useful for analysis. I developed a method to filter time series, a machine learning model to classify whether time series are oscillatory, and an autocorrelation method to examine the periodicity of time series data. My experimental results show that yeast cells show oscillations in the fluorescence of flavins. Specifically, I show that in high glucose conditions, cells generate flavin oscillations asynchronously within a population, and these flavin oscillations couple with the cell division cycle. I show that cells can individually reset the phase of their flavin oscillations in response to abrupt nutrient changes, independently of the cell division cycle. I also show that deletion strains generate flavin oscillations that exhibit different behaviour from dissolved oxygen oscillations from chemostat conditions. Finally, I use flux balance analysis to address whether proteomic constraints in cellular metabolism mean that temporal partitioning of biosynthesis is advantageous for the yeast cell, and whether such partitioning explains the timing of the metabolic cycle. My results show that under proteomic constraints, it is advantageous for the cell to sequentially synthesise biomass components because doing so shortens the timescale of biomass synthesis. However, the degree of advantage of sequential over parallel biosynthesis is lower when both carbon and nitrogen sources are limiting. This thesis thus confirms autonomous generation of flavin oscillations, and suggests a model in which the YMC responds to nutrient conditions and subsequently entrains the cell division cycle. It also emphasises the possibility that subpopulations in the culture explain chemostat-based observations of the YMC. Furthermore, this thesis paves the way for using computational methods to analyse large datasets of oscillatory time series, which is useful for various fields of study beyond the YMC

    Climate Change and Critical Agrarian Studies

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    Climate change is perhaps the greatest threat to humanity today and plays out as a cruel engine of myriad forms of injustice, violence and destruction. The effects of climate change from human-made emissions of greenhouse gases are devastating and accelerating; yet are uncertain and uneven both in terms of geography and socio-economic impacts. Emerging from the dynamics of capitalism since the industrial revolution — as well as industrialisation under state-led socialism — the consequences of climate change are especially profound for the countryside and its inhabitants. The book interrogates the narratives and strategies that frame climate change and examines the institutionalised responses in agrarian settings, highlighting what exclusions and inclusions result. It explores how different people — in relation to class and other co-constituted axes of social difference such as gender, race, ethnicity, age and occupation — are affected by climate change, as well as the climate adaptation and mitigation responses being implemented in rural areas. The book in turn explores how climate change – and the responses to it - affect processes of social differentiation, trajectories of accumulation and in turn agrarian politics. Finally, the book examines what strategies are required to confront climate change, and the underlying political-economic dynamics that cause it, reflecting on what this means for agrarian struggles across the world. The 26 chapters in this volume explore how the relationship between capitalism and climate change plays out in the rural world and, in particular, the way agrarian struggles connect with the huge challenge of climate change. Through a huge variety of case studies alongside more conceptual chapters, the book makes the often-missing connection between climate change and critical agrarian studies. The book argues that making the connection between climate and agrarian justice is crucial

    La traduzione specializzata all’opera per una piccola impresa in espansione: la mia esperienza di internazionalizzazione in cinese di Bioretics© S.r.l.

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    Global markets are currently immersed in two all-encompassing and unstoppable processes: internationalization and globalization. While the former pushes companies to look beyond the borders of their country of origin to forge relationships with foreign trading partners, the latter fosters the standardization in all countries, by reducing spatiotemporal distances and breaking down geographical, political, economic and socio-cultural barriers. In recent decades, another domain has appeared to propel these unifying drives: Artificial Intelligence, together with its high technologies aiming to implement human cognitive abilities in machinery. The “Language Toolkit – Le lingue straniere al servizio dell’internazionalizzazione dell’impresa” project, promoted by the Department of Interpreting and Translation (Forlì Campus) in collaboration with the Romagna Chamber of Commerce (Forlì-Cesena and Rimini), seeks to help Italian SMEs make their way into the global market. It is precisely within this project that this dissertation has been conceived. Indeed, its purpose is to present the translation and localization project from English into Chinese of a series of texts produced by Bioretics© S.r.l.: an investor deck, the company website and part of the installation and use manual of the Aliquis© framework software, its flagship product. This dissertation is structured as follows: Chapter 1 presents the project and the company in detail; Chapter 2 outlines the internationalization and globalization processes and the Artificial Intelligence market both in Italy and in China; Chapter 3 provides the theoretical foundations for every aspect related to Specialized Translation, including website localization; Chapter 4 describes the resources and tools used to perform the translations; Chapter 5 proposes an analysis of the source texts; Chapter 6 is a commentary on translation strategies and choices

    Deteção de intrusões de rede baseada em anomalias

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    Dissertação de mestrado integrado em Eletrónica Industrial e ComputadoresAo longo dos últimos anos, a segurança de hardware e software tornou-se uma grande preocupação. À medida que a complexidade dos sistemas aumenta, as suas vulnerabilidades a sofisticadas técnicas de ataque têm proporcionalmente escalado. Frequentemente o problema reside na heterogenidade de dispositivos conectados ao veículo, tornando difícil a convergência da monitorização de todos os protocolos num único produto de segurança. Por esse motivo, o mercado requer ferramentas mais avançadas para a monitorizar ambientes críticos à vida humana, tais como os nossos automóveis. Considerando que existem várias formas de interagir com os sistemas de entretenimento do automóvel como o Bluetooth, o Wi-fi ou CDs multimédia, a necessidade de auditar as suas interfaces tornou-se uma prioridade, uma vez que elas representam um sério meio de aceeso à rede interna do carro. Atualmente, os mecanismos de segurança de um carro focam-se na monitotização da rede CAN, deixando para trás as tecnologias referidas e não contemplando os sistemas não críticos. Como exemplo disso, o Bluetooth traz desafios diferentes da rede CAN, uma vez que interage diretamente com o utilizador e está exposto a ataques externos. Uma abordagem alternativa para tornar o automóvel num sistema mais robusto é manter sob supervisão as comunicações que com este são estabelecidas. Ao implementar uma detecção de intrusão baseada em anomalias, esta dissertação visa analisar o protocolo Bluetooth no sentido de identificar interações anormais que possam alertar para uma situação fora dos padrões de utilização. Em última análise, este produto de software embebido incorpora uma grande margem de auto-aprendizagem, que é vital para enfrentar quaisquer ameaças desconhecidas e aumentar os níveis de segurança globais. Ao longo deste documento, apresentamos o estudo do problema seguido de uma metodologia alternativa que implementa um algoritmo baseado numa LSTM para prever a sequência de comandos HCI correspondentes a tráfego Bluetooth normal. Os resultados mostram a forma como esta abordagem pode impactar a deteção de intrusões nestes ambientes ao demonstrar uma grande capacidade para identificar padrões anómalos no conjunto de dados considerado.In the last few years, hardware and software security have become a major concern. As the systems’ complexity increases, its vulnerabilities to several sophisticated attack techniques have escalated likewise. Quite often, the problem lies in the heterogeneity of the devices connected to the vehicle, making it difficult to converge the monitoring systems of all existing protocols into one security product. Thereby, the market requires more refined tools to monitor life-risky environments such as personal vehicles. Considering that there are several ways to interact with the car’s infotainment system, such as Wi-fi, Bluetooth, or CD player, the need to audit these interfaces has become a priority as they represent a serious channel to reach the internal car network. Nowadays, security in car networks focuses on CAN bus monitoring, leaving behind the aforementioned technologies and not contemplating other non-critical systems. As an example of these concerns, Bluetooth brings different challenges compared to CAN as it interacts directly with the user, being exposed to external attacks. An alternative approach to converting modern vehicles and their set of computers into more robust systems is to keep track of established communications with them. By enforcing anomaly-based intrusion detection this dissertation aims to analyze the Bluetooth protocol to identify abnormal user interactions that may alert for a non conforming pattern. Ultimately, such embedded software product incorporates a self-learning edge, which is vital to face newly developed threats and increasing global security levels. Throughout this document, we present the study case followed by an alternative methodology that implements an LSTM based algorithm to predict a sequence of HCI commands corresponding to normal Bluetooth traffic. The results show how this approach can impact intrusion detection in such environments by expressing a high capability of identifying abnormal patterns in the considered data

    Serverless Cloud Computing: A Comparative Analysis of Performance, Cost, and Developer Experiences in Container-Level Services

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    Serverless cloud computing is a subset of cloud computing considerably adopted to build modern web applications, while the underlying server and infrastructure management duties are abstracted from customers to the cloud vendors. In serverless computing, customers must pay for the runtime consumed by their services, but they are exempt from paying for the idle time. Prior to serverless containers, customers needed to provision, scale, and manage servers, which was a bottleneck for rapidly growing customer-facing applications where latency and scaling were a concern. The viability of adopting a serverless platform for a web application regarding performance, cost, and developer experiences is studied in this thesis. Three serverless container-level services are employed in this study from AWS and GCP. The services include GCP Cloud Run, GKE AutoPilot, and AWS EKS with AWS Fargate. Platform as a Service (PaaS) underpins the former, and Container as a Service (CaaS) the remainder. A single-page web application was created to perform incremental and spike load tests on those services to assess the performance differences. Furthermore, the cost differences are compared and analyzed. Lastly, the final element considered while evaluating the developer experiences is the complexity of using the services during the project implementation. Based on the results of this research, it was determined that PaaS-based solutions are a high-performing, affordable alternative for CaaS-based solutions in circumstances where high levels of traffic are periodically anticipated, but sporadic latency is never a concern. Given that this study has limitations, the author recommends additional research to strengthen it
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