Compressed sensing in fluorescence microscopy.

Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse problems, from under-sampled data with respect to the Nyquist criterium. CS exploits sparsity constraints based on the knowledge of prior information, relative to the structure of the object in the spatial or other domains. It is commonly used in image and video compression as well as in scientific and medical applications, including computed tomography and magnetic resonance imaging. In the field of fluorescence microscopy, it has been demonstrated to be valuable for fast and high-resolution imaging, from single-molecule localization, super-resolution to light-sheet microscopy. Furthermore, CS has found remarkable applications in the field of mesoscopic imaging, facilitating the study of small animals' organs and entire organisms. This review article illustrates the working principles of CS, its implementations in optical imaging and discusses several relevant uses of CS in the field of fluorescence imaging from super-resolution microscopy to mesoscopy

Seven Defining Features of Terahertz (THz) Wireless Systems: A Fellowship of Communication and Sensing

Wireless communication at the terahertz (THz) frequency bands (0.1-10THz) is viewed as one of the cornerstones of tomorrow's 6G wireless systems. Owing to the large amount of available bandwidth, THz frequencies can potentially provide wireless capacity performance gains and enable high-resolution sensing. However, operating a wireless system at the THz-band is limited by a highly uncertain channel. Effectively, these channel limitations lead to unreliable intermittent links as a result of a short communication range, and a high susceptibility to blockage and molecular absorption. Consequently, such impediments could disrupt the THz band's promise of high-rate communications and high-resolution sensing capabilities. In this context, this paper panoramically examines the steps needed to efficiently deploy and operate next-generation THz wireless systems that will synergistically support a fellowship of communication and sensing services. For this purpose, we first set the stage by describing the fundamentals of the THz frequency band. Based on these fundamentals, we characterize seven unique defining features of THz wireless systems: 1) Quasi-opticality of the band, 2) THz-tailored wireless architectures, 3) Synergy with lower frequency bands, 4) Joint sensing and communication systems, 5) PHY-layer procedures, 6) Spectrum access techniques, and 7) Real-time network optimization. These seven defining features allow us to shed light on how to re-engineer wireless systems as we know them today so as to make them ready to support THz bands. Furthermore, these features highlight how THz systems turn every communication challenge into a sensing opportunity. Ultimately, the goal of this article is to chart a forward-looking roadmap that exposes the necessary solutions and milestones for enabling THz frequencies to realize their potential as a game changer for next-generation wireless systems.Comment: 26 pages, 6 figure

Compressed Sensing for Open-ended Waveguide Non-Destructive Testing and Evaluation

Ph. D. ThesisNon-destructive testing and evaluation (NDT&E) systems using open-ended waveguide (OEW) suffer from critical challenges. In the sensing stage, data acquisition is time-consuming by raster scan, which is difficult for on-line detection. Sensing stage also disregards demand for the latter feature extraction process, leading to an excessive amount of data and processing overhead for feature extraction. In the feature extraction stage, efficient and robust defect region segmentation in the obtained image is challenging for a complex image background. Compressed sensing (CS) demonstrates impressive data compression ability in various applications using sparse models. How to develop CS models in OEW NDT&E that jointly consider sensing & processing for fast data acquisition, data compression, efficient and robust feature extraction is remaining challenges. This thesis develops integrated sensing-processing CS models to address the drawbacks in OEW NDT systems and carries out their case studies in low-energy impact damage detection for carbon fibre reinforced plastics (CFRP) materials. The major contributions are: (1) For the challenge of fast data acquisition, an online CS model is developed to offer faster data acquisition and reduce data amount without any hardware modification. The images obtained with OEW are usually smooth which can be sparsely represented with discrete cosine transform (DCT) basis. Based on this information, a customised 0/1 Bernoulli matrix for CS measurement is designed for downsampling. The full data is reconstructed with orthogonal matching pursuit algorithm using the downsampling data, DCT basis, and the customised 0/1 Bernoulli matrix. It is hard to determine the sampling pixel numbers for sparse reconstruction when lacking training data, to address this issue, an accumulated sampling and recovery process is developed in this CS model. The defect region can be extracted with the proposed histogram threshold edge detection (HTED) algorithm after each recovery, which forms an online process. A case study in impact damage detection on CFRP materials is carried out for validation. The results show that the data acquisition time is reduced by one order of magnitude while maintaining equivalent image quality and defect region as raster scan. (2) For the challenge of efficient data compression that considers the later feature extraction, a feature-supervised CS data acquisition method is proposed and evaluated. It reserves interested features while reducing the data amount. The frequencies which reveal the feature only occupy a small part of the frequency band, this method finds these sparse frequency range firstly to supervise the later sampling process. Subsequently, based on joint sparsity of neighbour frame and the extracted frequency band, an aligned spatial-spectrum sampling scheme is proposed. The scheme only samples interested frequency range for required features by using a customised 0/1 Bernoulli measurement matrix. The interested spectral-spatial data are reconstructed jointly, which has much faster speed than frame-by-frame methods. The proposed feature-supervised CS data acquisition is implemented and compared with raster scan and the traditional CS reconstruction in impact damage detection on CFRP materials. The results show that the data amount is reduced greatly without compromising feature quality, and the gain in reconstruction speed is improved linearly with the number of measurements. (3) Based on the above CS-based data acquisition methods, CS models are developed to directly detect defect from CS data rather than using the reconstructed full spatial data. This method is robust to texture background and more time-efficient that HTED algorithm. Firstly, based on the histogram is invariant to down-sampling using the customised 0/1 Bernoulli measurement matrix, a qualitative method which only gives binary judgement of defect is developed. High probability of detection and accuracy is achieved compared to other methods. Secondly, a new greedy algorithm of sparse orthogonal matching pursuit (spOMP)-based defect region segmentation method is developed to quantitatively extract the defect region, because the conventional sparse reconstruction algorithms cannot properly use the sparse character of correlation between the measurement matrix and CS data. The proposed algorithms are faster and more robust to interference than other algorithms.China Scholarship Counci

Novel Hybrid-Learning Algorithms for Improved Millimeter-Wave Imaging Systems

Increasing attention is being paid to millimeter-wave (mmWave), 30 GHz to 300 GHz, and terahertz (THz), 300 GHz to 10 THz, sensing applications including security sensing, industrial packaging, medical imaging, and non-destructive testing. Traditional methods for perception and imaging are challenged by novel data-driven algorithms that offer improved resolution, localization, and detection rates. Over the past decade, deep learning technology has garnered substantial popularity, particularly in perception and computer vision applications. Whereas conventional signal processing techniques are more easily generalized to various applications, hybrid approaches where signal processing and learning-based algorithms are interleaved pose a promising compromise between performance and generalizability. Furthermore, such hybrid algorithms improve model training by leveraging the known characteristics of radio frequency (RF) waveforms, thus yielding more efficiently trained deep learning algorithms and offering higher performance than conventional methods. This dissertation introduces novel hybrid-learning algorithms for improved mmWave imaging systems applicable to a host of problems in perception and sensing. Various problem spaces are explored, including static and dynamic gesture classification; precise hand localization for human computer interaction; high-resolution near-field mmWave imaging using forward synthetic aperture radar (SAR); SAR under irregular scanning geometries; mmWave image super-resolution using deep neural network (DNN) and Vision Transformer (ViT) architectures; and data-level multiband radar fusion using a novel hybrid-learning architecture. Furthermore, we introduce several novel approaches for deep learning model training and dataset synthesis.Comment: PhD Dissertation Submitted to UTD ECE Departmen

Novel Approaches for Nondestructive Testing and Evaluation

Nondestructive testing and evaluation (NDT&E) is one of the most important techniques for determining the quality and safety of materials, components, devices, and structures. NDT&E technologies include ultrasonic testing (UT), magnetic particle testing (MT), magnetic flux leakage testing (MFLT), eddy current testing (ECT), radiation testing (RT), penetrant testing (PT), and visual testing (VT), and these are widely used throughout the modern industry. However, some NDT processes, such as those for cleaning specimens and removing paint, cause environmental pollution and must only be considered in limited environments (time, space, and sensor selection). Thus, NDT&E is classified as a typical 3D (dirty, dangerous, and difficult) job. In addition, NDT operators judge the presence of damage based on experience and subjective judgment, so in some cases, a flaw may not be detected during the test. Therefore, to obtain clearer test results, a means for the operator to determine flaws more easily should be provided. In addition, the test results should be organized systemically in order to identify the cause of the abnormality in the test specimen and to identify the progress of the damage quantitatively

Pattern identification of biomedical images with time series: contrasting THz pulse imaging with DCE-MRIs

Objective We provide a survey of recent advances in biomedical image analysis and classification from emergent imaging modalities such as terahertz (THz) pulse imaging (TPI) and dynamic contrast-enhanced magnetic resonance images (DCE-MRIs) and identification of their underlining commonalities. Methods Both time and frequency domain signal pre-processing techniques are considered: noise removal, spectral analysis, principal component analysis (PCA) and wavelet transforms. Feature extraction and classification methods based on feature vectors using the above processing techniques are reviewed. A tensorial signal processing de-noising framework suitable for spatiotemporal association between features in MRI is also discussed. Validation Examples where the proposed methodologies have been successful in classifying TPIs and DCE-MRIs are discussed. Results Identifying commonalities in the structure of such heterogeneous datasets potentially leads to a unified multi-channel signal processing framework for biomedical image analysis. Conclusion The proposed complex valued classification methodology enables fusion of entire datasets from a sequence of spatial images taken at different time stamps; this is of interest from the viewpoint of inferring disease proliferation. The approach is also of interest for other emergent multi-channel biomedical imaging modalities and of relevance across the biomedical signal processing community

Microwave Sensing and Imaging

In recent years, microwave sensing and imaging have acquired an ever-growing importance in several applicative fields, such as non-destructive evaluations in industry and civil engineering, subsurface prospection, security, and biomedical imaging. Indeed, microwave techniques allow, in principle, for information to be obtained directly regarding the physical parameters of the inspected targets (dielectric properties, shape, etc.) by using safe electromagnetic radiations and cost-effective systems. Consequently, a great deal of research activity has recently been devoted to the development of efficient/reliable measurement systems, which are effective data processing algorithms that can be used to solve the underlying electromagnetic inverse scattering problem, and efficient forward solvers to model electromagnetic interactions. Within this framework, this Special Issue aims to provide some insights into recent microwave sensing and imaging systems and techniques