Nanodiamond-Gutta Percha Composite Biomaterials for Root Canal Therapy.

Root canal therapy (RCT) represents a standard of treatment that addresses infected pulp tissue in teeth and protects against future infection. RCT involves removing dental pulp comprising blood vessels and nerve tissue, decontaminating residually infected tissue through biomechanical instrumentation, and root canal obturation using a filler material to replace the space that was previously composed of dental pulp. Gutta percha (GP) is typically used as the filler material, as it is malleable, inert, and biocompatible. While filling the root canal space with GP is the standard of care for endodontic therapies, it has exhibited limitations including leakage, root canal reinfection, and poor mechanical properties. To address these challenges, clinicians have explored the use of alternative root filling materials other than GP. Among the classes of materials that are being explored as novel endodontic therapy platforms, nanodiamonds (NDs) may offer unique advantages due to their favorable properties, particularly for dental applications. These include versatile faceted surface chemistry, biocompatibility, and their role in improving mechanical properties, among others. This study developed a ND-embedded GP (NDGP) that was functionalized with amoxicillin, a broad-spectrum antibiotic commonly used for endodontic infection. Comprehensive materials characterization confirmed improved mechanical properties of NDGP over unmodified GP. In addition, digital radiography and microcomputed tomography imaging demonstrated that obturation of root canals with NDGP could be achieved using clinically relevant techniques. Furthermore, bacterial growth inhibition assays confirmed drug functionality of NDGP functionalized with amoxicillin. This study demonstrates a promising path toward NDGP implementation in future endodontic therapy for improved treatment outcomes

Optically Active Rare-Earth Doped Films Synthesized by Pulsed Laser Deposition for Biomedical Applications

Optically active materials are used in many biomedical applications ranging from medical imaging to light therapies. Investigating the effects of differing nanostructure configurations on the optical performance of these materials can improve tunability, efficiency, and practicality for their respective applications. This work utilizes pulsed laser deposition (PLD) to develop nanostructured thin films and determines their optical performance for applications in computed radiography for medical imaging and in LEDs which can be used in biomedical applications such as photobiomodulation. In computed radiography, scattering of the stimulation light by the storage phosphor crystal grain boundaries in imaging plates negatively impacts spatial resolution. Storage phosphor plates with thinner phosphor layers have been developed to reduce scattering distance and increase spatial resolution, although at the expense of reduced x-ray absorption. A transparent or translucent nanostructured film, containing a much higher percentage of storage phosphor crystals achievable in bulk glass-ceramic materials made by conventional methods, may have acceptable photostimulated luminescence efficiency and imaging performance characteristics greater than commercial imaging plates. In an attempt to achieve a nanostructured film with superior performance in x-ray imaging, a glass-ceramic imaging plate for computed radiography was synthesized via PLD for the first time. The imaging plate was comprised of Eu-doped BaCl2 crystallites and an amorphous matrix. Nanolayered films comprising of BaF2, Eu2O3, and Al2O3 were synthesized via PLD with differing layered configurations to manipulate the coordinate surrounds of the europium dopant and determine its effects on optical properties. TEM cross-section analysis was conducted to verify the desired nano-layering. Different post-deposition heat treatments were investigated, and the films were evaluated for applications as a phosphor layer for UV-pumped white light LEDs which can be used for solid-state lighting and biomedical light therapies. A Mn dopant was added to europium to discover the threshold for the amount of manganese necessary to optically influence the nanolayered films. Although Mn/Eu co-doping did not prove advantageous for white light LEDs, all nanostructures of Eu-doped films have the potential for the desired application. Nanoscale control of optically-active thin films was demonstrated using pulsed laser deposition. Determining the effects of differing nanostructures on optical properties can lead to improvements in certain biomedical applications

Deep learning in medical imaging and radiation therapy

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146980/1/mp13264_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146980/2/mp13264.pd

Interdisciplinary crossover for rapid advancements: Collaboration between medical and engineering scientists with the focus on Serbia

Napretku medicine su poslednjih decenija veoma mnogo doprineli pronalasci iz različitih oblasti inženjerstva. Polovinom dvadesetog veka uspostavlja se nova naučna oblast, biomedicinsko inženjerstvo (BI), koje se do sada razvilo u veoma složenu naučnu disciplinu koja je zahtevala i poseban obrazovni profil. Na univerzitetima širom sveta, kao i na nekoliko univerziteta u Srbiji ustanovljeni su različiti programi iz oblasti biomedicinskog inženjerstva. Takođe, u nekoliko naučnih institucija u Srbiji sprovode se intenzivna istraživanja u ovoj oblasti. U ovom radu prikazani su rezultati istraživanja nekoliko grupa naučnika iz oblasti inženjerstva i medicine sa ciljem da se ilustruje koliko je široko polje istraživanja u oblasti biomedicinskog inženjerstva i kakve su mogućnosti njihove primene u dijagnostici i lečenju različitih bolesti.Over the past decades, development of engineering sciences has vastly contributed to advancements in medicine by production of numerous devices for diagnostics and treatment. In the middle of the 20th century, a new scientific field, biomedical engineering (BE), was established, which has developed into an extremely complex scientific discipline requiring a distinctive educational profile. Various study programs in BE have been established at universities around the world but also at several universities in Serbia. Also, intensive research in this field is performed at several scientific institutions in Serbia. In the present paper, short summaries of the research results of several groups of engineers and medical doctors are presented as an illustration of the wide field of BE research and possibilities of its application in diagnosis and therapy of various diseases

Inorganic, Organic, and Perovskite Halides with Nanotechnology for High-Light Yield X- and γ-Ray Scintillators

Trends in scintillators that are used in many applications, such as medical imaging, security, oil-logging, high energy physics and non-destructive inspections are reviewed. First, we address traditional inorganic and organic scintillators with respect of limitation in the scintillation light yields and lifetimes. The combination of high–light yield and fast response can be found in Ce 3+ , Pr 3+ and Nd 3+ lanthanide-doped scintillators while the maximum light yield conversion of 100,000 photons/MeV can be found in Eu 3+ doped SrI 2 . However, the fabrication of those lanthanide-doped scintillators is inefficient and expensive as it requires high-temperature furnaces. A self-grown single crystal using solution processes is already introduced in perovskite photovoltaic technology and it can be the key for low-cost scintillators. A novel class of materials in scintillation includes lead halide perovskites. These materials were explored decades ago due to the large X-ray absorption cross section. However, lately lead halide perovskites have become a focus of interest due to recently reported very high photoluminescence quantum yield and light yield conversion at low temperatures. In principle, 150,000–300,000 photons/MeV light yields can be proportional to the small energy bandgap of these materials, which is below 2 eV. Finally, we discuss the extraction efficiency improvements through the fabrication of the nanostructure in scintillators, which can be implemented in perovskite materials. The recent technology involving quantum dots and nanocrystals may also improve light conversion in perovskite scintillators

Interdisciplinary crossover for rapid advancements - collaboration between medical and engineering scientists with the focus on Serbia

Over the past decades, development of engineering sciences has vastly contributed to advancements in medicine by production of numerous devices for diagnostics and treatment. In the middle of the 20th century, a new scientific field, biomedical engineering (BE), was established, which has developed into an extremely complex scientific discipline requiring a distinctive educational profile. Various study programs in BE have been established at universities around the world but also at several universities in Serbia. Also, intensive research in this field is performed at several scientific institutions in Serbia. In the present paper, short summaries of the research results of several groups of engineers and medical doctors are presented as an illustration of the wide field of BE research and possibilities of its application in diagnosis and therapy of various diseases

Size and shape specific particles toward biomedical imaging: design, fabrication, and characterization

Thesis (Ph.D.)--Boston UniversityThe power of a biomedical imaging modality can be augmented and is, in large part, determined by the capabilities of the available contrast agents. For example, quantum dots represent a colorful palette of powerful contrast agents for optical fluorescence imaging and Raman spectroscopy, given their tunable multiplexing capability and long-term stability compared to traditional organic molecule-based fluorescent labels. On the contrary, as the workhorses in both clinical and research imaging, the full potentials of magnetic resonance imaging and computed tomography have yet to be actualized due to several existing fundamental limitations in the currently available contrast agents, including but not limited to, the lack of multiplexing capability, low sensitivity, as well as the lack of functional imaging capacity. Leveraging both traditional top-down micro- and nanoelectromechanical systems fabrication techniques and bottom-up self-assembly approaches, this dissertation explores the possibility of mitigating these limitations by engineering precisely controllable, size and shape (as well as a host of other materials properties) specific micro- and nanoparticles, for use as the next generation contrast agents for magnetic resonance imaging and computed tomography. Herein, the ways by which engineering approaches can impact the design, fabrication and characterization of contrast agents is investigated. Specifically, different configmations of magnetic micro- and nanoparticles, including double-disk and hollow-cylinder structmes, fabricated using a top-down approach were employed as magnetic resonance imaging contrast agents enabled with a multiplexing capability and improved sensitivity. Subsequently, a scalable nanomanufactming platform, utilizing nanoporous anodized aluminum oxide membranes as templates for pattern transfer as well as thermal/ultraviolet nanoimprinting techniques, was developed for the high throughput fabrication of size and shape specific polymeric nanorods. When ladened with X-ray attenuating tantalum oxide nanoparticle payloads, these polymeric nanorods can be used as contrast agents for computed tomography, yielding prolonged vascular circulation times, improved sensitivity, as well as targeted imaging capabilities. Furthermore, by applying various payload materials, this nanomanufacturing platform also has the flexibility to produce contrast agents for other imaging modalities, as well as the potential to realize dual-purpose agents for both diagnostic and therapeutic applications

Recent developments in X-ray diffraction/scattering computed tomography for materials science

X-ray diffraction/scattering computed tomography (XDS-CT) methods are a non-destructive class of chemical imaging techniques that have the capacity to provide reconstructions of sample cross-sections with spatially resolved chemical information. While X-ray diffraction CT (XRD-CT) is the most well-established method, recent advances in instrumentation and data reconstruction have seen greater use of related techniques like small angle X-ray scattering CT and pair distribution function CT. Additionally, the adoption of machine learning techniques for tomographic reconstruction and data analysis are fundamentally disrupting how XDS-CT data is processed. The following narrative review highlights recent developments and applications of XDS-CT with a focus on studies in the last five years. This article is part of the theme issue 'Exploring the length scales, timescales and chemistry of challenging materials (Part 2)'

Recent developments in X-ray diffraction/scattering computed tomography for materials science

X-ray diffraction/scattering computed tomography (XDS-CT) methods are a non-destructive class of chemical imaging techniques that have the capacity to provide reconstructions of sample cross-sections with spatially resolved chemical information. While X-ray diffraction CT (XRD-CT) is the most well-established method, recent advances in instrumentation and data reconstruction have seen greater use of related techniques like small angle X-ray scattering CT and pair distribution function CT. Additionally, the adoption of machine learning techniques for tomographic reconstruction and data analysis are fundamentally disrupting how XDS-CT data is processed. The following narrative review highlights recent developments and applications of XDS-CT with a focus on studies in the last five years. This article is part of the theme issue 'Exploring the length scales, timescales and chemistry of challenging materials (Part 2)'

Knee cartilage segmentation using multi purpose interactive approach

Interactive model incorporates expert interpretation and automated segmentation. However, cartilage has complicated structure, indistinctive tissue contrast in magnetic resonance image of knee hardens image review and existing interactive methods are sensitive to various technical problems such as bi-label segmentation problem, shortcut problem and sensitive to image noise. Moreover, redundancy issue caused by non-cartilage labelling has never been tackled. Therefore, Bi-Bezier Curve Contrast Enhancement is developed to improve visual quality of magnetic resonance image by considering brightness preservation and contrast enhancement control. Then, Multipurpose Interactive Tool is developed to handle users’ interaction through Label Insertion Point approach. Approximate NonCartilage Labelling system is developed to generate computerized non-cartilage label, while preserves cartilage for expert labelling. Both computerized and interactive labels initialize Random Walks based segmentation model. To evaluate contrast enhancement techniques, Measure of Enhancement (EME), Absolute Mean Brightness Error (AMBE) and Feature Similarity Index (FSIM) are used. The results suggest that Bi-Bezier Curve Contrast Enhancement outperforms existing methods in terms of contrast enhancement control (EME = 41.44±1.06), brightness distortion (AMBE = 14.02±1.29) and image quality (FSIM = 0.92±0.02). Besides, implementation of Approximate Non-Cartilage Labelling model has demonstrated significant efficiency improvement in segmenting normal cartilage (61s±8s, P = 3.52 x 10-5) and diseased cartilage (56s±16s, P = 1.4 x 10-4). Finally, the proposed labelling model has high Dice values (Normal: 0.94±0.022, P = 1.03 x 10-9; Abnormal: 0.92±0.051, P = 4.94 x 10-6) and is found to be beneficial to interactive model (+0.12)