Antibodies for immunolabeling by light and electron microscopy : not for the faint hearted

Reliable antibodies represent crucial tools in the arsenal of the cell biologist and using them to localize antigens for immunocytochemistry is one of their most important applications. However, antibody-antigen interactions are much more complex and unpredictable than suggested by the old 'lock and key' analogy, and the goal of trying to prove that an antibody is specific is far more difficult than is generally appreciated. Here, we discuss the problems associated with the very complicated issue of trying to establish that an antibody (and the results obtained with it) is specific for the immunolabeling approaches used in light or electron microscopy. We discuss the increasing awareness that significant numbers of commercial antibodies are often not up to the quality required. We provide guidelines for choosing and testing antibodies in immuno-EM. Finally, we describe how quantitative EM methods can be used to identify reproducible patterns of antibody labeling and also extract specific labeling distributions.Peer reviewe

Emergence of Tuning to Natural Stimulus Statistics along the Central Auditory Pathway

We have previously shown that neurons in primary auditory cortex (A1) of anaesthetized (ketamine/medetomidine) ferrets respond more strongly and reliably to dynamic stimuli whose statistics follow "natural" 1/f dynamics than to stimuli exhibiting pitch and amplitude modulations that are faster (1/f(0.5)) or slower (1/f(2)) than 1/f. To investigate where along the central auditory pathway this 1/f-modulation tuning arises, we have now characterized responses of neurons in the central nucleus of the inferior colliculus (ICC) and the ventral division of the mediate geniculate nucleus of the thalamus (MGV) to 1/f(gamma) distributed stimuli with gamma varying between 0.5 and 2.8. We found that, while the great majority of neurons recorded from the ICC showed a strong preference for the most rapidly varying (1/f(0.5) distributed) stimuli, responses from MGV neurons did not exhibit marked or systematic preferences for any particular gamma exponent. Only in A1 did a majority of neurons respond with higher firing rates to stimuli in which gamma takes values near 1. These results indicate that 1/f tuning emerges at forebrain levels of the ascending auditory pathway

Test targets 5.0: A Collaborative effort exploring the use of scientific methods for color imaging and process control

Test Targets is about scholarship that intimately involves faculty and students in the process of writing and publishing. It is a collection if research papers that require collaborative effort over a time span of three academic quarters. Initially, students learned metrology, color management system, and the use of test targets for device optimization and process control. As time goes by, students are encouraged to identify research topics, formulate methodologies, and carry out experiments and data analyses in order to have specific findings. - p.

Applying market shaping approaches to increase access to assistive technology in low- and middle-income countries

Development outcomes are inextricably linked to the health of the marketplace that delivers products and services to people in low- and middle-income countries (LMIC). Shortcomings in the market for assistive technology (AT) contribute to low access in LMIC. Market shaping is aimed at improving a market's specific outcomes, such as access to high quality, affordable AT, by targeting the root causes of these shortcomings. The paper summarizes the findings of market analyses conducted under the UK aid funded AT2030 programme in support of ATscale and aims to discuss how market shaping can help more people gain access to the AT that they need and what are the best mechanisms to unlock markets and commercial opportunity in LMICs. The paper also explores how market shaping for AT markets could be part of a mission-oriented approach AT policy. A mission-oriented approach can help accelerate progress toward a common objective among stakeholders, at country or global level. While market-shaping activities direct the outcomes of the market toward a specific end goal, such as access to quality, affordable products and services, missions are more comprehensive and include other policy interventions and stakeholder collaborations in order to create a robust and sustainable structure

Network Performance in HTML5 Video Connections

[EN] Currently, most of remote education systems use video streaming as the main basis to support teaching. These emissions can be seen in devices with different hardware features such as personal computers, tablets or smartphones through networks with different capacities. The use of different web browsers and coding options can also influence the network performance. Therefore, the quality of the video displayed may be different. This work presents a practical study to establish the best combination of web browsers and containers to encode multimedia files for videos streaming in personal computers running Windows 7 and Windows 10 operating systems. For this, a video encoded with different codecs and compressed with different containers have been transmitted through a 1000BaseT network. Finally, the results are analyzed and compared to determine which would be the most efficient combination of parameters according to the resolution of the transmitted video.This work has been partially supported by the European Union through the ERANETMED (Euromediterranean Cooperation through ERANET joint activities and beyond) project ERANETMED3-227 SMARTWATIR and by the Ministerio de Educación, Cultura y Deporte , through the Convocatoria 2016 - Proyectos I+D+I - Programa Estatal De Investigación, Desarrollo e Innovación Orientada a los retos de la sociedad (Project TEC2016-76795-C6-4-R) and through the Convocatoria 2017 - Proyectos I+D+I - Programa Estatal de Investigación, Desarrollo e Innovación, convocatoria excelencia (Project TIN2017-84802-C2-1-P).Sendra, S.; Túnez-Murcia, AI.; Lloret, J.; Jimenez, JM. (2018). Network Performance in HTML5 Video Connections. Network Protocols and Algorithms. 10(3):43-62. https://doi.org/10.5296/npa.v10i3.13933S436210

Stress during transition from home to public childcare

Four saliva probes were collected per day from 104 children (10 to 35 months old) transitioning from home (T0) to childcare across a four-month period (until T3), resulting in over one thousand cortisol values. Latent Profile Analysis classified three profiles within a regular spectrum of children’s cortisol rhythms and described a fourth hypocortisol stress profile. Further Latent Transition Analysis revealed that profiles frequently changed across the transition but stabilized at T3. Most importantly, regular profiles across transition most likely occurred with high AQS scores of mother-child and care provider–child attachment. A machine learning procedure (XGBoost) featured predictors for stress profiles at T3 (when the child ought to be adjusted and stress profiles should be rare) referring to characteristics of the children (e.g., gender, number of siblings, peer contact before entry), the mothers (their worries), the care providers (their work experience, engagement, attachment) and the groups in the childcare centers (e.g., size, age differences, illness frequency). As a result, experience with siblings and peers before entry facilitated the transition. However, most conditions not linearly affecting children’s cortisol revealed even opposite effects when analyzed at different times. For example, smaller group size and large age-differences at T1 helped the child to stabilize a Regular profile, perhaps due to better control over the situation and greater support from the older children in the group. At T3, however, Regular profiles were associated with larger group size and smaller age-differences which might be helpful for establishing close peer relationships to buffer stress

2019 Abstract Booklet

Complete Schedule of Events for the 21st Annual Undergraduate Research Symposium at Minnesota State University, Mankato