Quantitative 3d reconstruction from scanning electron microscope images based on affine camera models

Scanning electron microscopes (SEMs) are versatile imaging devices for the micro-and nanoscale that find application in various disciplines such as the characterization of biological, mineral or mechanical specimen. Even though the specimen’s two-dimensional (2D) properties are provided by the acquired images, detailed morphological characterizations require knowledge about the three-dimensional (3D) surface structure. To overcome this limitation, a reconstruction routine is presented that allows the quantitative depth reconstruction from SEM image sequences. Based on the SEM’s imaging properties that can be well described by an affine camera, the proposed algorithms rely on the use of affine epipolar geometry, self-calibration via factorization and triangulation from dense correspondences. To yield the highest robustness and accuracy, different sub-models of the affine camera are applied to the SEM images and the obtained results are directly compared to confocal laser scanning microscope (CLSM) measurements to identify the ideal parametrization and underlying algorithms. To solve the rectification problem for stereo-pair images of an affine camera so that dense matching algorithms can be applied, existing approaches are adapted and extended to further enhance the yielded results. The evaluations of this study allow to specify the applicability of the affine camera models to SEM images and what accuracies can be expected for reconstruction routines based on self-calibration and dense matching algorithms. © MDPI AG. All rights reserved

Calibration routine for a telecentric stereo vision system considering affine mirror ambiguity

A robust calibration approach for a telecentric stereo camera system for three-dimensional (3-D) surface measurements is presented, considering the effect of affine mirror ambiguity. By optimizing the parameters of a rigid body transformation between two marker planes and transforming the two-dimensional (2-D) data into one coordinate frame, a 3-D calibration object is obtained, avoiding high manufacturing costs. Based on the recent contributions in the literature, the calibration routine consists of an initial parameter estimation by affine reconstruction to provide good start values for a subsequent nonlinear stereo refinement based on a Levenberg–Marquardt optimization. To this end, the coordinates of the calibration target are reconstructed in 3-D using the Tomasi–Kanade factorization algorithm for affine cameras with Euclidean upgrade. The reconstructed result is not properly scaled and not unique due to affine ambiguity. In order to correct the erroneous scaling, the similarity transformation between one of the 2-D calibration plane points and the corresponding 3-D points is estimated. The resulting scaling factor is used to rescale the 3-D point data, which then allows in combination with the 2-D calibration plane data for a determination of the start values for the subsequent nonlinear stereo refinement. As the rigid body transformation between the 2-D calibration planes is also obtained, a possible affine mirror ambiguity in the affine reconstruction result can be robustly corrected. The calibration routine is validated by an experimental calibration and various plausibility tests. Due to the usage of a calibration object with metric information, the determined camera projection matrices allow for a triangulation of correctly scaled metric 3-D points without the need for an individual camera magnification determination

A decomposition method for non-rigid structure from motion with orthographic cameras

Session: Video Processing, Analysis and Applications + AnimationIn this paper, we propose a new approach to non-rigid structure from motion based on the trajectory basis method by decomposing the problem into two sub-problems. The existing trajectory basis method requires the number of trajectory basis vectors to be specified beforehand, and then camera motion and the non-rigid structure are recovered simultaneously. However, we observe that the camera motion can be derived from a mean shape without recovering the non-rigid structure. Hence, the camera motion can be recovered as a sub-problem to optimize an error indicator without a full recovery of the non-rigid structure or the need to pre-define the number of basis required for describing the non-rigid structure. With the camera motion recovered, the non-rigid structure can then be solved in a second sub-problem together with the determination of the basis number by minimizing another error indicator. The solutions to these two sub-problems can be combined to solve the non-rigid structure from motion problem in an automatic manner, without any need to pre-define the number of basis vectors. Experiments show that the proposed method improves the reconstruction quality of both the non-rigid structure and camera motion.postprin

Virtual View Networks for Object Reconstruction

All that structure from motion algorithms "see" are sets of 2D points. We show that these impoverished views of the world can be faked for the purpose of reconstructing objects in challenging settings, such as from a single image, or from a few ones far apart, by recognizing the object and getting help from a collection of images of other objects from the same class. We synthesize virtual views by computing geodesics on novel networks connecting objects with similar viewpoints, and introduce techniques to increase the specificity and robustness of factorization-based object reconstruction in this setting. We report accurate object shape reconstruction from a single image on challenging PASCAL VOC data, which suggests that the current domain of applications of rigid structure-from-motion techniques may be significantly extended

Occlusion-Aware Multi-View Reconstruction of Articulated Objects for Manipulation

The goal of this research is to develop algorithms using multiple views to automatically recover complete 3D models of articulated objects in unstructured environments and thereby enable a robotic system to facilitate further manipulation of those objects. First, an algorithm called Procrustes-Lo-RANSAC (PLR) is presented. Structure-from-motion techniques are used to capture 3D point cloud models of an articulated object in two different configurations. Procrustes analysis, combined with a locally optimized RANSAC sampling strategy, facilitates a straightforward geometric approach to recovering the joint axes, as well as classifying them automatically as either revolute or prismatic. The algorithm does not require prior knowledge of the object, nor does it make any assumptions about the planarity of the object or scene. Second, with such a resulting articulated model, a robotic system is then able to manipulate the object either along its joint axes at a specified grasp point in order to exercise its degrees of freedom or move its end effector to a particular position even if the point is not visible in the current view. This is one of the main advantages of the occlusion-aware approach, because the models capture all sides of the object meaning that the robot has knowledge of parts of the object that are not visible in the current view. Experiments with a PUMA 500 robotic arm demonstrate the effectiveness of the approach on a variety of real-world objects containing both revolute and prismatic joints. Third, we improve the proposed approach by using a RGBD sensor (Microsoft Kinect) that yield a depth value for each pixel immediately by the sensor itself rather than requiring correspondence to establish depth. KinectFusion algorithm is applied to produce a single high-quality, geometrically accurate 3D model from which rigid links of the object are segmented and aligned, allowing the joint axes to be estimated using the geometric approach. The improved algorithm does not require artificial markers attached to objects, yields much denser 3D models and reduces the computation time

Scalable Dense Monocular Surface Reconstruction

This paper reports on a novel template-free monocular non-rigid surface reconstruction approach. Existing techniques using motion and deformation cues rely on multiple prior assumptions, are often computationally expensive and do not perform equally well across the variety of data sets. In contrast, the proposed Scalable Monocular Surface Reconstruction (SMSR) combines strengths of several algorithms, i.e., it is scalable with the number of points, can handle sparse and dense settings as well as different types of motions and deformations. We estimate camera pose by singular value thresholding and proximal gradient. Our formulation adopts alternating direction method of multipliers which converges in linear time for large point track matrices. In the proposed SMSR, trajectory space constraints are integrated by smoothing of the measurement matrix. In the extensive experiments, SMSR is demonstrated to consistently achieve state-of-the-art accuracy on a wide variety of data sets.Comment: International Conference on 3D Vision (3DV), Qingdao, China, October 201

Deformable 3-D Modelling from Uncalibrated Video Sequences

Submitted for the degree of Doctor of Philosophy, Queen Mary, University of Londo

Relative Affine Structure: Canonical Model for 3D from 2D Geometry and Applications

We propose an affine framework for perspective views, captured by a single extremely simple equation based on a viewer-centered invariant we call "relative affine structure". Via a number of corollaries of our main results we show that our framework unifies previous work --- including Euclidean, projective and affine --- in a natural and simple way, and introduces new, extremely simple, algorithms for the tasks of reconstruction from multiple views, recognition by alignment, and certain image coding applications

Self-calibration and motion recovery from silhouettes with two mirrors

LNCS v. 7724-7727 (pts. 1-4) entitled: Computer vision - ACCV 2012: 11th Asian Conference on Computer Vision ... 2012: revised selected papersThis paper addresses the problem of self-calibration and motion recovery from a single snapshot obtained under a setting of two mirrors. The mirrors are able to show five views of an object in one image. In this paper, the epipoles of the real and virtual cameras are firstly estimated from the intersection of the bitangent lines between corresponding images, from which we can easily derive the horizon of the camera plane. The imaged circular points and the angle between the mirrors can then be obtained from equal angles between the bitangent lines, by planar rectification. The silhouettes produced by reflections can be treated as a special circular motion sequence. With this observation, technique developed for calibrating a circular motion sequence can be exploited to simplify the calibration of a single-view two-mirror system. Different from the state-of-the-art approaches, only one snapshot is required in this work for self-calibrating a natural camera and recovering the poses of the two mirrors. This is more flexible than previous approaches which require at least two images. When more than a single image is available, each image can be calibrated independently and the problem of varying focal length does not complicate the calibration problem. After the calibration, the visual hull of the objects can be obtained from the silhouettes. Experimental results show the feasibility and the preciseness of the proposed approach. © 2013 Springer-Verlag.postprin