Hyperspectral colon tissue cell classification

A novel algorithm to discriminate between normal and malignant tissue cells of the human colon is presented. The microscopic level images of human colon tissue cells were acquired using hyperspectral imaging technology at contiguous wavelength intervals of visible light. While hyperspectral imagery data provides a wealth of information, its large size normally means high computational processing complexity. Several methods exist to avoid the so-called curse of dimensionality and hence reduce the computational complexity. In this study, we experimented with Principal Component Analysis (PCA) and two modifications of Independent Component Analysis (ICA). In the first stage of the algorithm, the extracted components are used to separate four constituent parts of the colon tissue: nuclei, cytoplasm, lamina propria, and lumen. The segmentation is performed in an unsupervised fashion using the nearest centroid clustering algorithm. The segmented image is further used, in the second stage of the classification algorithm, to exploit the spatial relationship between the labeled constituent parts. Experimental results using supervised Support Vector Machines (SVM) classification based on multiscale morphological features reveal the discrimination between normal and malignant tissue cells with a reasonable degree of accuracy

Application of spectral and spatial indices for specific class identification in Airborne Prism EXperiment (APEX) imaging spectrometer data for improved land cover classification

Hyperspectral remote sensing's ability to capture spectral information of targets in very narrow bandwidths gives rise to many intrinsic applications. However, the major limiting disadvantage to its applicability is its dimensionality, known as the Hughes Phenomenon. Traditional classification and image processing approaches fail to process data along many contiguous bands due to inadequate training samples. Another challenge of successful classification is to deal with the real world scenario of mixed pixels i.e. presence of more than one class within a single pixel. An attempt has been made to deal with the problems of dimensionality and mixed pixels, with an objective to improve the accuracy of class identification. In this paper, we discuss the application of indices to cope with the disadvantage of the dimensionality of the Airborne Prism EXperiment (APEX) hyperspectral Open Science Dataset (OSD) and to improve the classification accuracy using the Possibilistic c–Means (PCM) algorithm. This was used for the formulation of spectral and spatial indices to describe the information in the dataset in a lesser dimensionality. This reduced dimensionality is used for classification, attempting to improve the accuracy of determination of specific classes. Spectral indices are compiled from the spectral signatures of the target and spatial indices have been defined using texture analysis over defined neighbourhoods. The classification of 20 classes of varying spatial distributions was considered in order to evaluate the applicability of spectral and spatial indices in the extraction of specific class information. The classification of the dataset was performed in two stages; spectral and a combination of spectral and spatial indices individually as input for the PCM classifier. In addition to the reduction of entropy, while considering a spectral-spatial indices approach, an overall classification accuracy of 80.50% was achieved, against 65% (spectral indices only) and 59.50% (optimally determined principal component

Hyperspectral Unmixing Overview: Geometrical, Statistical, and Sparse Regression-Based Approaches

Imaging spectrometers measure electromagnetic energy scattered in their instantaneous field view in hundreds or thousands of spectral channels with higher spectral resolution than multispectral cameras. Imaging spectrometers are therefore often referred to as hyperspectral cameras (HSCs). Higher spectral resolution enables material identification via spectroscopic analysis, which facilitates countless applications that require identifying materials in scenarios unsuitable for classical spectroscopic analysis. Due to low spatial resolution of HSCs, microscopic material mixing, and multiple scattering, spectra measured by HSCs are mixtures of spectra of materials in a scene. Thus, accurate estimation requires unmixing. Pixels are assumed to be mixtures of a few materials, called endmembers. Unmixing involves estimating all or some of: the number of endmembers, their spectral signatures, and their abundances at each pixel. Unmixing is a challenging, ill-posed inverse problem because of model inaccuracies, observation noise, environmental conditions, endmember variability, and data set size. Researchers have devised and investigated many models searching for robust, stable, tractable, and accurate unmixing algorithms. This paper presents an overview of unmixing methods from the time of Keshava and Mustard's unmixing tutorial [1] to the present. Mixing models are first discussed. Signal-subspace, geometrical, statistical, sparsity-based, and spatial-contextual unmixing algorithms are described. Mathematical problems and potential solutions are described. Algorithm characteristics are illustrated experimentally.Comment: This work has been accepted for publication in IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensin