2,436 research outputs found

    1. Helgoland Power and Energy Conference - 24. Dresdener Kreis 2023

    Get PDF
    Der Sammelband "1. Helgoland Power and Energy Conference" beinhaltet neben einem kurzen Bericht zum 24. Treffen des Dresdener Kreises 2023 wissenschaftliche BeitrĂ€ge von Doktoranden der beteiligten Hochschulinstitute zum Thema Elektroenergieversorgung. Der Dresdener Kreis setzt sich aus der Professur fĂŒr Elektroenergieversorgung der Technischen UniversitĂ€t Dresden, dem Fachgebiet Elektrische Anlagen und Netze der UniversitĂ€t Duisburg-Essen, dem Fachgebiet Elektrische Energieversorgung der Leibniz UniversitĂ€t Hannover und dem Lehrstuhl Elektrische Netze und Erneuerbare Energie der Otto-von-Guericke UniversitĂ€t Magdeburg zusammen und trifft sich einmal im Jahr zum fachlichen Austausch an einer der beteiligten UniversitĂ€ten

    Single-cell time-series analysis of metabolic rhythms in yeast

    Get PDF
    The yeast metabolic cycle (YMC) is a biological rhythm in budding yeast (Saccharomyces cerevisiae). It entails oscillations in the concentrations and redox states of intracellular metabolites, oscillations in transcript levels, temporal partitioning of biosynthesis, and, in chemostats, oscillations in oxygen consumption. Most studies on the YMC have been based on chemostat experiments, and it is unclear whether YMCs arise from interactions between cells or are generated independently by each cell. This thesis aims at characterising the YMC in single cells and its response to nutrient and genetic perturbations. Specifically, I use microfluidics to trap and separate yeast cells, then record the time-dependent intensity of flavin autofluorescence, which is a component of the YMC. Single-cell microfluidics produces a large amount of time series data. Noisy and short time series produced from biological experiments restrict the computational tools that are useful for analysis. I developed a method to filter time series, a machine learning model to classify whether time series are oscillatory, and an autocorrelation method to examine the periodicity of time series data. My experimental results show that yeast cells show oscillations in the fluorescence of flavins. Specifically, I show that in high glucose conditions, cells generate flavin oscillations asynchronously within a population, and these flavin oscillations couple with the cell division cycle. I show that cells can individually reset the phase of their flavin oscillations in response to abrupt nutrient changes, independently of the cell division cycle. I also show that deletion strains generate flavin oscillations that exhibit different behaviour from dissolved oxygen oscillations from chemostat conditions. Finally, I use flux balance analysis to address whether proteomic constraints in cellular metabolism mean that temporal partitioning of biosynthesis is advantageous for the yeast cell, and whether such partitioning explains the timing of the metabolic cycle. My results show that under proteomic constraints, it is advantageous for the cell to sequentially synthesise biomass components because doing so shortens the timescale of biomass synthesis. However, the degree of advantage of sequential over parallel biosynthesis is lower when both carbon and nitrogen sources are limiting. This thesis thus confirms autonomous generation of flavin oscillations, and suggests a model in which the YMC responds to nutrient conditions and subsequently entrains the cell division cycle. It also emphasises the possibility that subpopulations in the culture explain chemostat-based observations of the YMC. Furthermore, this thesis paves the way for using computational methods to analyse large datasets of oscillatory time series, which is useful for various fields of study beyond the YMC

    Beam scanning by liquid-crystal biasing in a modified SIW structure

    Get PDF
    A fixed-frequency beam-scanning 1D antenna based on Liquid Crystals (LCs) is designed for application in 2D scanning with lateral alignment. The 2D array environment imposes full decoupling of adjacent 1D antennas, which often conflicts with the LC requirement of DC biasing: the proposed design accommodates both. The LC medium is placed inside a Substrate Integrated Waveguide (SIW) modified to work as a Groove Gap Waveguide, with radiating slots etched on the upper broad wall, that radiates as a Leaky-Wave Antenna (LWA). This allows effective application of the DC bias voltage needed for tuning the LCs. At the same time, the RF field remains laterally confined, enabling the possibility to lay several antennas in parallel and achieve 2D beam scanning. The design is validated by simulation employing the actual properties of a commercial LC medium

    Markov field models of molecular kinetics

    Get PDF
    Computer simulations such as molecular dynamics (MD) provide a possible means to understand protein dynamics and mechanisms on an atomistic scale. The resulting simulation data can be analyzed with Markov state models (MSMs), yielding a quantitative kinetic model that, e.g., encodes state populations and transition rates. However, the larger an investigated system, the more data is required to estimate a valid kinetic model. In this work, we show that this scaling problem can be escaped when decomposing a system into smaller ones, leveraging weak couplings between local domains. Our approach, termed independent Markov decomposition (IMD), is a first-order approximation neglecting couplings, i.e., it represents a decomposition of the underlying global dynamics into a set of independent local ones. We demonstrate that for truly independent systems, IMD can reduce the sampling by three orders of magnitude. IMD is applied to two biomolecular systems. First, synaptotagmin-1 is analyzed, a rapid calcium switch from the neurotransmitter release machinery. Within its C2A domain, local conformational switches are identified and modeled with independent MSMs, shedding light on the mechanism of its calcium-mediated activation. Second, the catalytic site of the serine protease TMPRSS2 is analyzed with a local drug-binding model. Equilibrium populations of different drug-binding modes are derived for three inhibitors, mirroring experimentally determined drug efficiencies. IMD is subsequently extended to an end-to-end deep learning framework called iVAMPnets, which learns a domain decomposition from simulation data and simultaneously models the kinetics in the local domains. We finally classify IMD and iVAMPnets as Markov field models (MFM), which we define as a class of models that describe dynamics by decomposing systems into local domains. Overall, this thesis introduces a local approach to Markov modeling that enables to quantitatively assess the kinetics of large macromolecular complexes, opening up possibilities to tackle current and future computational molecular biology questions

    Towards trustworthy computing on untrustworthy hardware

    Get PDF
    Historically, hardware was thought to be inherently secure and trusted due to its obscurity and the isolated nature of its design and manufacturing. In the last two decades, however, hardware trust and security have emerged as pressing issues. Modern day hardware is surrounded by threats manifested mainly in undesired modifications by untrusted parties in its supply chain, unauthorized and pirated selling, injected faults, and system and microarchitectural level attacks. These threats, if realized, are expected to push hardware to abnormal and unexpected behaviour causing real-life damage and significantly undermining our trust in the electronic and computing systems we use in our daily lives and in safety critical applications. A large number of detective and preventive countermeasures have been proposed in literature. It is a fact, however, that our knowledge of potential consequences to real-life threats to hardware trust is lacking given the limited number of real-life reports and the plethora of ways in which hardware trust could be undermined. With this in mind, run-time monitoring of hardware combined with active mitigation of attacks, referred to as trustworthy computing on untrustworthy hardware, is proposed as the last line of defence. This last line of defence allows us to face the issue of live hardware mistrust rather than turning a blind eye to it or being helpless once it occurs. This thesis proposes three different frameworks towards trustworthy computing on untrustworthy hardware. The presented frameworks are adaptable to different applications, independent of the design of the monitored elements, based on autonomous security elements, and are computationally lightweight. The first framework is concerned with explicit violations and breaches of trust at run-time, with an untrustworthy on-chip communication interconnect presented as a potential offender. The framework is based on the guiding principles of component guarding, data tagging, and event verification. The second framework targets hardware elements with inherently variable and unpredictable operational latency and proposes a machine-learning based characterization of these latencies to infer undesired latency extensions or denial of service attacks. The framework is implemented on a DDR3 DRAM after showing its vulnerability to obscured latency extension attacks. The third framework studies the possibility of the deployment of untrustworthy hardware elements in the analog front end, and the consequent integrity issues that might arise at the analog-digital boundary of system on chips. The framework uses machine learning methods and the unique temporal and arithmetic features of signals at this boundary to monitor their integrity and assess their trust level

    Communication‐less Synchronous Rectification for In Motion Wireless Charging

    Get PDF
    This thesis puts forward a control scheme to allow for synchronous rectification for dynamic wireless power transfer. The automotive industry is transitioning away from internal combustion engines (ICEs) and towards electric vehicles (EVs). This transition is spurred by the environmental and economic benefits EVs offer over ICEs. However, further improvements can still be made to how electric vehicles operate. One of these improvements is the technology of in motion wireless charging or dynamic wireless power transfer. In motion wireless charging offers the ability to remove existing range anxiety concerns for EVs. It also offers the potential for a reduction in battery sizes for EVs, which are the primary cost of EVs, this in turn decreases the total costs of mass EV adoption. Traditional implementations of in motion wireless charging utilize passive rectification to simplify controls between embedded primary pads and the vehicle. However, this solution while effective, limits the potential benefits of wireless charging. The use of synchronous or active rectification techniques, offer improved performance, control techniques, and bidirectional capabilities. However, the reason synchronous rectification is not already used in in motion charging is the complexity of synchronization over wireless communication. To move past this challenge, this thesis investigates a synchronization scheme that can be achieved without communication by taking advantage of induced free resonant currents in the vehicle’s tuning network to synchronize the switching transitions to receive power. In this thesis a traditional in motion wireless charging system utilizing passive rectification is designed and built as a benchmark for dynamic charging. Simulations of this control scheme are presented. Practical considerations are addressed for hardware realization. Finally, the control approach is validated through hardware in static and dynamic applications

    (b2023 to 2014) The UNBELIEVABLE similarities between the ideas of some people (2006-2016) and my ideas (2002-2008) in physics (quantum mechanics, cosmology), cognitive neuroscience, philosophy of mind, and philosophy (this manuscript would require a REVOLUTION in international academy environment!)

    Get PDF
    (b2023 to 2014) The UNBELIEVABLE similarities between the ideas of some people (2006-2016) and my ideas (2002-2008) in physics (quantum mechanics, cosmology), cognitive neuroscience, philosophy of mind, and philosophy (this manuscript would require a REVOLUTION in international academy environment!
    • 

    corecore