Optimal Experiment Design for the Identification of One Module in the Interconnection of Locally Controlled Systems

International audienceIn this paper, we consider the problem of designing the least costly experiment that leads to a sufficiently accurate estimate of one module in a network of locally controlled systems. A module in such a network can be identified by exciting the corresponding local closed loop system. Such an excitation signal will not only perturb the input/output of the to-be-identified module, but also other modules due to the interconnection. Consequently, the cost of the identification can be expressed as the sum of the influence of the excitation signal on the inputs and outputs of all locally controlled systems. We develop a methodology to design the spectrum of the excitation signal in such a way that this cost is minimized while guaranteeing a certain accuracy for the identified model. We also propose an alternative identification configuration which can further reduce the propagation of the excitation signal to other modules and we make steps to robustify this optimal experiment design problem with respect to the cost of the identification

Actuation attacks on constrained linear systems: A set-theoretic analysis

Cuenta con un 2º editor: Incluido en el Volumen 53, Issue 2 Article number 145388This paper considers a constrained discrete-time linear system subject to actuation attacks. The attacks are modelled as false data injections to the system, such that the total input (control input plus injection) satisfies hard input constraints. We establish a sufficient condition under which it is not possible to maintain the states of the system within a compact state constraint set for all possible realizations of the actuation attack. The developed condition is a simple function of the spectral radius of the system, the relative sizes of the input and state constraint sets, and the proportion of the input constraint set allowed to the attacker.Ministerio de Economía y Competitividad (España) DPI2017-86918-RJapan Society for the Promotion of Science PE1604

engineering methionine γ lyase from citrobacter freundii for anticancer activity

Abstract Methionine deprivation of cancer cells, which are deficient in methionine biosynthesis, has been envisioned as a therapeutic strategy to reduce cancer cell viability. Methionine γ-lyase (MGL), an enzyme that degrades methionine, has been exploited to selectively remove the amino acid from cancer cell environment. In order to increase MGL catalytic activity, we performed sequence and structure conservation analysis of MGLs from various microorganisms. Whereas most of the residues in the active site and at the dimer interface were found to be conserved, residues located in the C-terminal flexible loop, forming a wall of the active site entry channel, were found to be variable. Therefore, we carried out site-saturation mutagenesis at four independent positions of the C-terminal flexible loop, P357, V358, P360 and A366 of MGL from Citrobacter freundii, generating libraries that were screened for activity. Among the active variants, V358Y exhibits a 1.9-fold increase in the catalytic rate and a 3-fold increase in KM, resulting in a catalytic efficiency similar to wild type MGL. V358Y cytotoxic activity was assessed towards a panel of cancer and nonmalignant cell lines and found to exhibit IC50 lower than the wild type. The comparison of the 3D-structure of V358Y MGL with other MGL available structures indicates that the C-terminal loop is either in an open or closed conformation that does not depend on the amino acid at position 358. Nevertheless, mutations at this position allosterically affects catalysis

Aural stealth for night vision portable imagers

Modern tactics for carrying out military and antiterrorist operations calls for the development of a new generation of enhanced portable infrared imagers. The high performance of these imagers relies on the focal plane arrays, which are maintained at cryogenic temperatures using rotary Stirling cryogenic engines. These engines are known as powerful sources of wideband vibration export. For the sake of weight and compactness, the enclosure of the above imager is usually designed in the form of a light metal thin-walled shell, accommodating a directly mounted Infrared Detector Dewar Cooler Assembly. The operation of the device typically leads to an excitation of the inherently lightly damped structural resonances and therefore, to a radiation of the specific acoustic signature capable of compromising the aural stealth of the IR imager. Such a noisy IR imager may be detected from quite a long distance using enhanced sniper detection equipment or even aurally spotted when used in a close proximity to the target. Numerous efforts were taken towards achieving the desired inaudibility level, apparently becoming one of a crucial figure of merit characterizing the portable IR imager. However, even the best examples of modern should-be silent imagers are quite audible from as far as 50 meters. The presented research intends to improve the aural stealth of the portable IR imager by using three different approaches: First, by compliantly mounting the Infrared Detector Dewar Cooler Assembly where the stiffness and damping of the vibration protective pad are optimized for the best acoustical performance without developing excessive line of sight jitter. Secondly, by using the concept of the weak radiator to reshape the enclosure mode shapes, and finally developing a multi-modal distributed dynamic absorber (MMDA) to enhance the absorption of the vibrating structure. The multi-modal characteristic of such a dynamic absorber makes it highly dynamically reactive through a wide frequency range (20 kHz) of excitation. It will be shown that incorporating a MMDA into the vibrating structure will result in ultra range vibration attenuation, making the IR aurally silent