Diurnal and Seasonal Solar Induced Chlorophyll Fluorescence and Photosynthesis in a Boreal Scots Pine Canopy

Solar induced chlorophyll fluorescence has been shown to be increasingly an useful proxy for the estimation of gross primary productivity (GPP), at a range of spatial scales. Here, we explore the seasonality in a continuous time series of canopy solar induced fluorescence (hereafter SiF) and its relation to canopy gross primary production (GPP), canopy light use efficiency (LUE), and direct estimates of leaf level photochemical efficiency in an evergreen canopy. SiF was calculated using infilling in two bands from the incoming and reflected radiance using a pair of Ocean Optics USB2000+ spectrometers operated in a dual field of view mode, sampling at a 30 min time step using custom written automated software, from early spring through until autumn in 2011. The optical system was mounted on a tower of 18 m height adjacent to an eddy covariance system, to observe a boreal forest ecosystem dominated by Scots pine. (Pinus sylvestris) A Walz MONITORING-PAM, multi fluorimeter system, was simultaneously mounted within the canopy adjacent to the footprint sampled by the optical system. Following correction of the SiF data for O2 and structural effects, SiF, SiF yield, LUE, the photochemicsl reflectance index (PRI), and the normalized difference vegetation index (NDVI) exhibited a seasonal pattern that followed GPP sampled by the eddy covariance system. Due to the complexities of solar azimuth and zenith angle (SZA) over the season on the SiF signal, correlations between SiF, SiF yield, GPP, and LUE were assessed on SZ

두 개의 기하학적 관찰 구성을 통합하는 자동화된 지상 기반 초 분광 시스템 개발

학위논문(석사) -- 서울대학교대학원 : 농업생명과학대학 협동과정 농림기상학, 2022. 8. 류영렬.Hyperspectral remote sensing is becoming a powerful tool for monitoring vegetation structure and functions. Especially, Sun-Induced chlorophyll fluorescence (SIF) and canopy reflectance monitoring have been widely used to understand physiological and structural changes in plants, and field spectroscopy has become established as an important technique for providing high spectral-, temporal resolution in-situ data as well as providing a means of scaling-up measurements from small areas to large areas. Recently, several tower-based remote sensing systems have been developed. However, in-situ studies have only monitored either BRF or BHR and there is still a lack of understanding of the geometric and optical differences in remote sensing observations, particularly between hemispheric-conical and bi-hemispheric configurations. Here, we developed an automated ground-based field spectroscopy system measuring far-red SIF and canopy hyperspectral reflectance (400–900 nm) with hemispherical-conical as well as bi-hemispherical configuration. To measure both bi-hemispherical and hemispherical-conical reflectance, we adopted a rotating prism by using a servo motor to face three types of ports that measure incoming-, outgoing irradiance and outgoing radiance. A white diffuse glass and collimating lens were used to measure the irradiance, and a collimating lens was used to measure the radiance with a field of view of 20 degrees. Additionally, we developed data management protocol that includes radiometric-, and wavelength calibrations. Finally, we report how BRF and BHR data differ in this system and investigated SIF and vegetation index from both hemispherical-conical and bi-hemispherical observation configurations for their ability to track GPP in the growing seasons of a deciduous broad-leaved forests.초 분광 원격 감지는 식생 구조와 기능을 모니터링하는 강력한 도구가 되고 있다. 특히, 식물의 생리적, 구조적 변화를 이해하기 위해 태양광 유도 엽록소 형광 (SIF)과 캐노피 반사율 모니터링이 널리 이용되고 있다. 현장 분광법은 높은 스펙트럼, 시간 분해능 현장 데이터를 제공하고 작은 영역에서 큰 영역으로 측정을 확장하는 수단을 제공하기 위한 중요한 기술로 확립되었다. 그러나, 수많은 연구가 현장 분광 시스템을 개발했지만, 반구-원추형 및 양 반구 구성 간의 원격 감지 관찰의 기하학적 및 광학적 차이에 대한 이해가 부족할 뿐만 아니라 초 분광 데이터를 지속적으로 수집하는 것은 여전히 어렵다. 우리는 반구형-원추형 및 이중 반구형 구성으로 원적외선 태양광 유도 엽록소 형광 및 캐노피 초 분광 반사율(400–900nm)을 측정하는 자동화된 지상 기반 필드 분광 시스템을 개발했다. 양방향 반사율과 반구형 원추형 반사율을 모두 측정하기 위해 서보 모터를 사용하여 프리즘을 회전하여 세가지 타입의 포트를 측정한다. 각 포트는 들어오는 복사 조도, 나가는 복사 조도 및 나가는 복사를 측정하는 세 가지 유형의 포트다. 조사조도는 백색확산유리와 굴절 렌즈를 사용하였고, 굴절 렌즈를 이용하여 조도를 측정하였다. 또한, 우리는 방사 측정 및 파장 교정을 포함하는 데이터 관리 프로토콜을 개발했다. 마지막으로, 우리는 낙엽 활엽수림의 성장기에 이 시스템에서 측정된 BRF와 BHR 데이터가 어떻게 다른지 보고하였다.Chapter 1. Introduction １ 1.1. Study Background １ 1.2. Purpose of Research ４ Chapter 2. Developing and Testing of Hyperspectral System ５ 2.1 Development of Hyperspectral System and Data Collecting ５ 2.1.1 The Central Control Unit and Spectrometer ５ 2.1.2 RotaPrism ７ 2.1.3 Data Collection ９ 2.3 Data Managing and Processing １１ 2.3.1 Preprocessing of Spectra １１ 2.3.2 Radiometric Calibration １３ 2.3.3 Retrieval of SIF and Vegetation Indices １５ 2.4 Ancillary Measurements to Monitoring Ecosystem. １７ Chapter 3. Application of Hyperspectral System １９ 3.1 Study Site １９ 3.2 Diurnal and Variation of Spectral Reflectance and SIF ２０ 3.3 Seasonal Variation of Vegetation Index and SIF ２２ 3.4 Broader Implications ２４ Chapter 4. Summary and Conclusions ２６ Bibliography ２８석

Scientific and technical challenges in remote sensing of plant canopy reflectance and fluorescence

State-of-the-art optical remote sensing of vegetation canopies is reviewed here to stimulate support from laboratory and field plant research. This overview of recent satellite spectral sensors and the methods used to retrieve remotely quantitative biophysical and biochemical characteristics of vegetation canopies shows that there have been substantial advances in optical remote sensing over the past few decades. Nevertheless, adaptation and transfer of currently available fluorometric methods aboard air- and space-borne platforms can help to eliminate errors and uncertainties in recent remote sensing data interpretation. With this perspective, red and blue-green fluorescence emission as measured in the laboratory and field is reviewed. Remotely sensed plant fluorescence signals have the potential to facilitate a better understanding of vegetation photosynthetic dynamics and primary production on a large scale. The review summarizes several scientific challenges that still need to be resolved to achieve operational fluorescence based remote sensing approache

Chlorophyll a fluorescence illuminates a path connecting plant molecular biology to Earth-system science

Remote sensing methods enable detection of solar-induced chlorophyll a fluorescence. However, to unleash the full potential of this signal, intensive cross-disciplinary work is required to harmonize biophysical and ecophysiological studies. For decades, the dynamic nature of chlorophyll a fluorescence (ChlaF) has provided insight into the biophysics and ecophysiology of the light reactions of photosynthesis from the subcellular to leaf scales. Recent advances in remote sensing methods enable detection of ChlaF induced by sunlight across a range of larger scales, from using instruments mounted on towers above plant canopies to Earth-orbiting satellites. This signal is referred to as solar-induced fluorescence (SIF) and its application promises to overcome spatial constraints on studies of photosynthesis, opening new research directions and opportunities in ecology, ecophysiology, biogeochemistry, agriculture and forestry. However, to unleash the full potential of SIF, intensive cross-disciplinary work is required to harmonize these new advances with the rich history of biophysical and ecophysiological studies of ChlaF, fostering the development of next-generation plant physiological and Earth-system models. Here, we introduce the scale-dependent link between SIF and photosynthesis, with an emphasis on seven remaining scientific challenges, and present a roadmap to facilitate future collaborative research towards new applications of SIF.Peer reviewe

Earth Observing System. Volume 1, Part 2: Science and Mission Requirements. Working Group Report Appendix

Areas of global hydrologic cycles, global biogeochemical cycles geophysical processes are addressed including biological oceanography, inland aquatic resources, land biology, tropospheric chemistry, oceanic transport, polar glaciology, sea ice and atmospheric chemistry

MODIS: Moderate-resolution imaging spectrometer. Earth observing system, volume 2B

The Moderate-Resolution Imaging Spectrometer (MODIS), as presently conceived, is a system of two imaging spectroradiometer components designed for the widest possible applicability to research tasks that require long-term (5 to 10 years), low-resolution (52 channels between 0.4 and 12.0 micrometers) data sets. The system described is preliminary and subject to scientific and technological review and modification, and it is anticipated that both will occur prior to selection of a final system configuration; however, the basic concept outlined is likely to remain unchanged