49,522 research outputs found
Laser Based Mid-Infrared Spectroscopic Imaging – Exploring a Novel Method for Application in Cancer Diagnosis
A number of biomedical studies have shown that mid-infrared spectroscopic images can provide
both morphological and biochemical information that can be used for the diagnosis of cancer. Whilst
this technique has shown great potential it has yet to be employed by the medical profession. By
replacing the conventional broadband thermal source employed in modern FTIR spectrometers with
high-brightness, broadly tuneable laser based sources (QCLs and OPGs) we aim to solve one of the
main obstacles to the transfer of this technology to the medical arena; namely poor signal to noise
ratios at high spatial resolutions and short image acquisition times. In this thesis we take the first
steps towards developing the optimum experimental configuration, the data processing algorithms
and the spectroscopic image contrast and enhancement methods needed to utilise these high
intensity laser based sources. We show that a QCL system is better suited to providing numerical
absorbance values (biochemical information) than an OPG system primarily due to the QCL pulse
stability. We also discuss practical protocols for the application of spectroscopic imaging to cancer
diagnosis and present our spectroscopic imaging results from our laser based spectroscopic imaging
experiments of oesophageal cancer tissue
A multi-object spectral imaging instrument
We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye
Detection of leaf structures in close-range hyperspectral images using morphological fusion
Close-range hyperspectral images are a promising source of information in plant biology, in particular, for in vivo study of physiological changes. In this study, we investigate how data fusion can improve the detection of leaf elements by combining pixel reflectance and morphological information. The detection of image regions associated to the leaf structures is the first step toward quantitative analysis on the physical effects that genetic manipulation, disease infections, and environmental conditions have in plants. We tested our fusion approach on Musa acuminata (banana) leaf images and compared its discriminant capability to similar techniques used in remote sensing. Experimental results demonstrate the efficiency of our fusion approach, with significant improvements over some conventional methods
Computationally Efficient Target Classification in Multispectral Image Data with Deep Neural Networks
Detecting and classifying targets in video streams from surveillance cameras
is a cumbersome, error-prone and expensive task. Often, the incurred costs are
prohibitive for real-time monitoring. This leads to data being stored locally
or transmitted to a central storage site for post-incident examination. The
required communication links and archiving of the video data are still
expensive and this setup excludes preemptive actions to respond to imminent
threats. An effective way to overcome these limitations is to build a smart
camera that transmits alerts when relevant video sequences are detected. Deep
neural networks (DNNs) have come to outperform humans in visual classifications
tasks. The concept of DNNs and Convolutional Networks (ConvNets) can easily be
extended to make use of higher-dimensional input data such as multispectral
data. We explore this opportunity in terms of achievable accuracy and required
computational effort. To analyze the precision of DNNs for scene labeling in an
urban surveillance scenario we have created a dataset with 8 classes obtained
in a field experiment. We combine an RGB camera with a 25-channel VIS-NIR
snapshot sensor to assess the potential of multispectral image data for target
classification. We evaluate several new DNNs, showing that the spectral
information fused together with the RGB frames can be used to improve the
accuracy of the system or to achieve similar accuracy with a 3x smaller
computation effort. We achieve a very high per-pixel accuracy of 99.1%. Even
for scarcely occurring, but particularly interesting classes, such as cars, 75%
of the pixels are labeled correctly with errors occurring only around the
border of the objects. This high accuracy was obtained with a training set of
only 30 labeled images, paving the way for fast adaptation to various
application scenarios.Comment: Presented at SPIE Security + Defence 2016 Proc. SPIE 9997, Target and
Background Signatures I
Uncertainty-Aware Organ Classification for Surgical Data Science Applications in Laparoscopy
Objective: Surgical data science is evolving into a research field that aims
to observe everything occurring within and around the treatment process to
provide situation-aware data-driven assistance. In the context of endoscopic
video analysis, the accurate classification of organs in the field of view of
the camera proffers a technical challenge. Herein, we propose a new approach to
anatomical structure classification and image tagging that features an
intrinsic measure of confidence to estimate its own performance with high
reliability and which can be applied to both RGB and multispectral imaging (MI)
data. Methods: Organ recognition is performed using a superpixel classification
strategy based on textural and reflectance information. Classification
confidence is estimated by analyzing the dispersion of class probabilities.
Assessment of the proposed technology is performed through a comprehensive in
vivo study with seven pigs. Results: When applied to image tagging, mean
accuracy in our experiments increased from 65% (RGB) and 80% (MI) to 90% (RGB)
and 96% (MI) with the confidence measure. Conclusion: Results showed that the
confidence measure had a significant influence on the classification accuracy,
and MI data are better suited for anatomical structure labeling than RGB data.
Significance: This work significantly enhances the state of art in automatic
labeling of endoscopic videos by introducing the use of the confidence metric,
and by being the first study to use MI data for in vivo laparoscopic tissue
classification. The data of our experiments will be released as the first in
vivo MI dataset upon publication of this paper.Comment: 7 pages, 6 images, 2 table
Learning Wavefront Coding for Extended Depth of Field Imaging
Depth of field is an important factor of imaging systems that highly affects
the quality of the acquired spatial information. Extended depth of field (EDoF)
imaging is a challenging ill-posed problem and has been extensively addressed
in the literature. We propose a computational imaging approach for EDoF, where
we employ wavefront coding via a diffractive optical element (DOE) and we
achieve deblurring through a convolutional neural network. Thanks to the
end-to-end differentiable modeling of optical image formation and computational
post-processing, we jointly optimize the optical design, i.e., DOE, and the
deblurring through standard gradient descent methods. Based on the properties
of the underlying refractive lens and the desired EDoF range, we provide an
analytical expression for the search space of the DOE, which is instrumental in
the convergence of the end-to-end network. We achieve superior EDoF imaging
performance compared to the state of the art, where we demonstrate results with
minimal artifacts in various scenarios, including deep 3D scenes and broadband
imaging
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